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RISS 인기검색어
- 검색키워드
- 저자 : Zihao Pan (검색결과 2 건)
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Circ_0001686 Promotes Prostate Cancer Progression by Up-Regulating SMAD3/TGFBR2 via miR-411-5p
Pan Jiancheng,Liu Zihao,Yang Zhizhao,Liang Enli,Fang Cheng,Zhang Dingrong,Zhou Xiaodong,Niu Yuanjie,Xin Zhongcheng,Chen Yegang,Cai Qiliang 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.1
Purpose: As the mechanism of interaction between circular RNAs (circRNAs) and microRNAs (miRNAs) in regulating the development of prostate cancer (PCa) is not clear, this study focuses on investigating these effects. Materials and Methods: Sample tissues were collected from the PCa of patients, and microarray analysis of human circRNAs was conducted. The expression of circ_0001686, hsa_miR-411-5p (miR-411-5p) were also detected by qRT-PCR. Circ_0001686 and miR-411-5p mimics were transfected into the PCa cell lines (CWR22RV1and LNCaP) and MTT, colony formation, Transwell, and scratch wound assays were used to analyze the biological behaviors of PCa cells. Si-circ_0001686 and ASO-miR-411-5p were used as negative controls, and dual-luciferase reporter assays were performed to verify the interactions among circ_0001686, miR-411-5p, and SMAD3/TGFBR2. The levels of SMAD3 and TGFBR2 in different treated PCa cells were measured by western blot, and in vivo experiments in a nude mouse model were carried out to strengthen the in vitro findings of miR-411-5p. Results: The expression of circ_0001686 was up-regulated, while the expression of miR-411-5p was down-regulated in PCa cells. Moreover, circ_0001686 promoted cell proliferation, migration, and invasion. Molecular mechanism exploration revealed that circ_0001686 could reduce miR-411-5p, affecting the downstream target genes of SMAD3 and TGFBR2. In vitro and in vivo studies verified that miR-411-5p inhibits PCa progression. Conclusions: Circ_0001686 can reduce miR-411-5p to increase the expression of SMAD3/TGFBR2, which consequently promotes the proliferation, invasion, and migration of PCa cells.
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Jiaxing Shen,Zihao Pan,Ping Xu,Yinghua Yu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7
The thermal performance of a steel-basalt fiber polymer concrete machine tool joint surface has a key influence on a machine tool. Based on virtual material theory, the equivalent thermal model was established and the thermal performance parameters of virtual materials were deduced by theoretical calculation. The calculation method of actual contact area ratio of steel - basalt fiber polymer concrete joint surface was established based on discrete principle and its accuracy was verified. To determine the influence of preload pressure on the actual contact area ratio, a prediction model was established. The thermal conductivity of the material was measured by the quasi-steady state method. A new systematic simulation analysis method that synthesizes virtual material theory and finite element simulation analysis was proposed, and its accuracy was verified. The results show that the relative error is only 5.01 %, which proves the method’s effectiveness.
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