EphB1 and Ephrin-B, New Potential Biomarkers for Squamous Cell/adenosquamous Carcinomas and Adenocarcinomas of the Gallbladder

Yuan, Yuan,Yang, Zhu-Lin,Miao, Xiong-Ying,Liu, Zi-Ru,Li, Dai-Qiang,Zou, Qiong,Li, Jing-He,Liang, Lu-Feng,Zeng, Gui-Xiang,Chen, Sen-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

Squamous cell/adenosquamous carcinoma (SC/ASC) of the gallbladder are rare tumors and there are few clinical reports in the literature. Herein we report our clinical experience with 46 patients with SC/ASC and 80 with adenocarcinoma (AC). Expression of EphB1 and Ephrin-B in each tumor was determined using immunohistochemical methods for determination of correlations with prognosis. There was no difference in EphB1 and Ephrin-B expression between SC/ASC and AC tumors (P>0.05), but greater expression in those less than 3 cm in diameter, stage I or II (TNM stage), with no lymph node metastases, with no local invasion and treated with radical resection was apparent. Expression of EphB1 (P<0.05) and Ephrin-B (P<0.01) was higher in well differentiated than in poorly differentiated AC tumors. Kaplan-Meier survival analysis indicated that degree of differentiation, tumor diameter, lymph node metastases, local invasion, surgical approach and expression rate of EphB1 and Ephrin-B were closely related to the survival of SC/ASC (P<0.05) and AC patients (P<0.01). Patients with tumors that positive expressed EphB1 and Ephrin-B, whether it is SC/ASC ($P_{SC/ASC}$ =0.000) or AC ($P_{AC}$ =0.000 or $P_{AC}$ =0.002) had longer survival than those negative expression. Cox multivariate analysis indicated a negative correlation between expression of EphB1 or Ephrin-B and overall survival. Hence, EphB1 and Ephrin-B could be regarded as independent good prognostic factorsand important biological markers for SC/ASC and AC of gallbladder.

Novel and Effective Almagate Enema for Hemorrhagic Chronic Radiation Proctitis and Risk Factors for Fistula Development

Yuan, Zi-Xu,Ma, Teng-Hui,Zhong, Qing-Hua,Wang, Huai-Ming,Yu, Xi-Hu,Qin, Qi-Yuan,Chu, Li-Li,Wang, Lei,Wang, Jian-Ping Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.2

Radiation proctitis is a common complication after radiotherapy for pelvic malignant tumors. This study was conducted to assess the efficacy of novel almagate enemas in hemorrhagic chronic radiation proctitis (CRP) and evaluate risk factors related to rectal deep ulcer or fistula secondary to CRP. All patients underwent a colonoscopy to confirm the diagnosis of CRP and symptoms were graded. Typical endoscopic and pathological images, risk factors, and quality of life were also recorded. A total of 59 patients were enrolled. Gynecological cancers composed 93.1% of the primary malignancies. Complete or obvious reduction of bleeding was observed in 90% (53/59) patients after almagate enema. The mean score of bleeding improved from 2.17 to 0.83 (P<0.001) after the enemas. The mean response time was 12 days. No adverse effects were found. Moreover, long-term successful rate in controlling bleeding was 69% and the quality of life was dramatically improved (P=0.001). The efficacy was equivalent to rectal sucralfate, but the almagate with its antacid properties acted more rapidly than sucralfate. Furthermore, we firstly found that moderate to severe anemia was the risk factor of CRP patients who developed rectal deep ulcer or fistulas (P= 0.015). We also found abnormal hyaline-like thick wall vessels, which revealed endarteritis obliterans and the fibrosis underlying this disease. These findings indicate that almagate enema is a novel effective, rapid and well-tolerated method for hemorrhagic CRP. Moderate to severe anemia is a risk factor for deep ulceration or fistula.

브랜드 아이덴티티에서 서비스아이덴티티 적용성 연구 -중국 CCTV 방송국을 중심으로-

이자원 ( Li Zi-yuan ),반영환 ( Pan Young-hwan ) 커뮤니케이션디자인학회(구 시각디자인학회) 2021 커뮤니케이션 디자인학연구 Vol.75 No.-

기업가치가 제품경제 주도에서 서비스 경제로 변화하면서 기업의 브랜드 아이덴티티는 더 이상 비주얼 제품 아이덴티티를 기초로 하지 않고 제품 서비스의 가치를 통해 소비자들에게 일종의 아이덴티티 신호를 전달하고 있다. 서비스 전략은 기존의 기업브랜드 아이덴티티 중에 가장 중요한 전략이 된다. 그러므로 본 논문은 서비스 차원에서 출발해 서비스 요소를 통한 서비스 아이덴티티를 구축하고 기업의 브랜드 아이덴티티 프레임(CBIM)에 통합시킨 후 새로운 기업브랜드 아이덴티티 프레임(CBISIM)를 구축하고 있다. 중국 TV 방송국은 소비자에게 서비스 가치를 제공하는 특수한 유형의 기업이기때문에 중국에서 제일 대표적인 중국CCTV 방송국을 예로 들어 본석을 통해, 브랜드 아이덴티티 관리에 서비스의 적합성을 설명함으로써 TV미디어 브랜드 아이덴티티 전략을 일정한 참고로 제공하고자 한다. With the leading value transforming from product economy to service economy, corporate brand identity is no longer based on visual product identity, instead, it delivers identification signal to consumers through the service value of the product. Service identity strategy has become the most important strategy in corporate brand identity today, thus, from the perspective of service, this article tries to build up elements of service identity and integrates it into the corporate brand identity framework (CBIM) to establish a new framework of corporate brand identity(CBISIM).TV Chinese TV station is a special type of enterprise that provides service value to consumers. Therefore, the most representative television station in China-CCTV is taken as a case analysis. It furtherly explains the applicability of service identity in CCTV channel brand identity management, providing reference for TV media brand identity strategy.

Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

Morphological Factors and Cardiac Doses in Whole Breast Radiation for Left-sided Breast Cancer

Guan, Hui,Dong, Yuan-Li,Ding, Li-Jie,Zhang, Zi-Cheng,Huang, Wei,Liu, Cheng-Xin,Fu, Cheng-Rui,Zhu, Jian,Li, Hong-Sheng,Li, Miao-Miao,Li, Bao-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Background: To investigate the impact of the breast size, shape, maximum heart depth (MDH), and chest wall hypotenuse (the distance connecting middle point of the sternum and the length of lung draw on the selected transverse CT slice) on the volumetric dose to heart with whole breast irradiation (WBI) of left-sided breast cancer patients. Materials and Methods: Fifty-three patients with left-sided breast cancer undergoing adjuvant intensity-modulated radiotherapy (IMRT) were enrolled in the study. The primary breast size and shape, MHD and DCWH (chest wall hypotenuse) were contoured on radiotherapy (RT) planning CT slices. The dose data of hearts were obtained from the dose-volume histograms (DVHs). Data were analyzed by one-way analysis of variance (ANOVA), Student's t-test and linear regression analysis. Results: Breast size was independent of heart dose, whereas breast shape, MHD and DCWH were correlated with heart dose. The shapes of breasts were divided into four types, as the flap type, hemisphere type, cone type and pendulous type with heart mean dose being $491.8{\pm}234.6cGy$, $752.7{\pm}219.0cGy$, $620.2{\pm}275.7cGy$, and $666.1{\pm}238.0cGy$, respectively. The flap type of breasts shows a strong statistically reduction in heart dose, compared to others (p=0.008 for V30 of heart). DCWH and MHD were found to be the most important parameters correlating with heart dose in WBI. Conclusions: More attention should be paid to the heart dose of non-flap type patients. The MHD was found to be the most important parameter to correlate with heart dose in tangential WBI, closely followed by the DCWH, which could help radiation oncologists and physicsts evaluate heart dose and design RT plan in advance.

Rapid and Visual Detection of Vibrio parahaemolyticus in Aquatic Foods Using blaC_ARB-17 Gene-Based Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick (LAMP-LFD)

( Yuan-qing Hu ),( Xian-hui Huang ),( Li-qing Guo ),( Zi-chen Shen ),( Lin-xue Lv ),( Feng-xia Li ),( Zan-hu Zhou ),( Dan-feng Zhang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.12

Vibrio parahaemolyticus is recognized as one of the most important foodborne pathogens responsible for gastroenteritis in humans. The bla_CARB-17 gene is an intrinsic β-lactamase gene and a novel species-specific genetic marker of V. parahaemolyticus. In this study, a loop-mediated isothermal amplification (LAMP) assay combined with a lateral flow dipstick (LFD) was developed targeting this bla_CARB-17 gene. The specificity of LAMP-LFD was ascertained by detecting V. parahaemolyticus ATCC 17802 and seven other non-V. parahaemolyticus strains. Finally, the practicability of LAMP-LFD was confirmed by detection with V. parahaemolyticus-contaminated samples and natural food samples. The results showed that the optimized reaction parameters of LAMP are as follows: 2.4 mmol/l Mg²⁺, 0.96 mmol/l dNTPs, 4.8 U Bst DNA polymerase, and an 8:1 ratio of inner primer to outer primer, at 63℃ for 40 min. The optimized reaction time of the LFD assay is 60 min. Cross-reactivity analysis with the seven non-V. parahaemolyticus strains showed that LAMP-LFD was exclusively specific for V. parahaemolyticus. The detection limit of LAMP-LFD for V. parahaemolyticus genomic DNA was 2.1 × 10^-4 ng/μl, corresponding to 630 fg/reaction and displaying a sensitivity that is 100-fold higher than that of conventional PCR. LAMP-LFD in a spiking study revealed a detection limit of approximately 6 CFU/ml, which was similar with conventional PCR. The developed LAMP-LFD specifically identified the 10 V. parahaemolyticus isolates from 30 seafood samples, suggesting that this LAMP-LFD may be a suitable diagnostic method for detecting V. parahaemolyticus in aquatic foods.

Hydrodynamic characteristics of a fixed semi-submersible platform interacting with incident waves by fully nonlinear method

Zhang Zi-Lin,Yuan Hong-Tao,Sun Shi-Li,Ren Hui-Long 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

Based on the potential flow theory, a fully nonlinear numerical procedure is developed with boundary element method to analyze the interaction between a fixed semi-submersible platform and incident waves in open water. The incident wave is separated from the scattered wave under fully nonlinear boundary conditions. The mixed Euler-Lagrangian method is used to capture the position of the disturbed wave surface in local coordinate systems. The wave forces exerted on an inverted conical frustum are used to ensure the accuracy of the present method and good agreements with published results are obtained. The hydrodynamic characteristics of the semi-submersible platform interacting with regular waves are analyzed. Pressure distribution with time and space, tension and compression of the platform under wave action are investigated. 3D behaviors of wave run-ups are predicted. Strong nonlinear phenomena such as wave upwelling and wave interference are observed and analyzed.

Impact of Global and Gene-Specific DNA Methylation in de Novo or Relapsed Acute Myeloid Leukemia Patients Treated with Decitabine

Zhang, Li-Ying,Yuan, You-Qing,Zhou, Dong-Ming,Wang, Zi-Yan,Ju, Song-Guang,Sun, Yu,Li, Jun,Fu, Jin-Xiang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.1

In this investigation, global DNA methylation patterns and the specific methylation status of 5 genes were studied in DNA from peripheral blood (PB) and impact on progression free survival (PFS) and overall-survival (OS) in patients with de novo or relapsed acute myeloid leukemia (AML) treated with decitabine-based regimens waas assessed. DNA was isolated from PB samples at the time of -1, 1, and 7 days of chemotherapy. Global methylation was determined by ELISA, and the CpG island DNA methylation profile of 5 genes using a DNA methylation PCR system. Our data demonstrated that patients with a high level of 5-mC had a poor prognosis after demethylation therapy and those who have low levels of 5-mC in PB achieved higher CR and better SO, but there was no significant correlation found between the 5-mC levels and other clinical features before treatment except the disease status. Higher methylation status of Sox2 and Oct4 genes was associated with differential response to demethylation therapy. A relatively low methylation percentage in one or both of these two genes was also associated with longer OS after decitabine based chemotherapy. We also suggest that global DNA and Oct-4/Sox2 methylation might impact on the pathogenesis of leukemia and play an important role in the initiation and progression. Moreover, dynamic analysis of 5-mC and Oct-4/Sox2 in peripheral blood nucleated cells of leukemia patients may provide clues to important molecular diagnostic and prognostic targets.

Selected microRNA-192 mutant indicates association with several function genes in bovine cells

Chen Zi,Dexin Zeng,Jiyong Zhou,Jianjun Dai,Luyan Jiang,Feng Xue,Yuan Jiang,Baoguang Li 한국유전학회 2018 Genes & Genomics Vol.40 No.4

MicroRNAs are implicated in many cellular processes such as cell differentiation and development, tumorigenesis, and immune regulation. In this study, miR192 was detected using quantitative real-time polymerase chain reaction (qRT-PCR) when MDBK cells were exposed to Escherichia coli. Cells with malfunction of bta-miR-192 were established using transcription activator-like effector nuclease (TALEN) technology. Finally, bta-miR-192 mutant cells were screened for differentially expressed genes using RNA-sequencing (RNA-seq). The results showed that miR192 significantly decreased in cells exposed to E. coli F18ac and E. coli K88ac. The RNA-seq results showed that 1673 differentially expressed transcripts were identified; 890 genes were upregulated and 775 genes were downregulated. With the gene ontology enrichment analysis, 431 differentially expressed genes (DEGs) were classified into 937 gene ontology terms. The pathway enrichment analysis showed that 535 genes were involved in 254 pathway terms. Interestingly, most of these DEGs were associated with the pathways in cancers or infectious diseases. When the selected DEGs (n = 162) in these pathways were intersected with 120 differential transcripts, 11 DEGs were identified. Subsequently, several genes associated with regulation, cancers, or viral infections, such as LEF1, AXIN2, MX1, and FCGR2B, were identified among the DEGs using functional analysis. Furthermore, associations between bta-miR-192 and DEGs were detected by intersecting the bta-miR-192’s target genes with the DEGs, indicating that three genes including CBL, DICER1 and TRERF1 were involved in this relationship. These findings provided useful guidance for investigating the role played by bta-miR-192 in cellular functionality in bovine cells.

A 4th-Order Wideband Sigma Delta Modulator Using Linear System Theory Technique

Ming-yuan Ren,Yong-sheng Zhang,Wei-kun Wu,Zhen-xi Bao,Zi-wei Zhao,Chao Li,Hong-guo Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.1

A 4th-Order Wideband Sigma Delta modulator structure is proposed in this paper, which uses linear system theory technique. There are many implementation techniques to improve the performance of Sigma Delta ADCs, such as noise coupling, differential sampling and dynamic elements matching. An extra loop delay is needed to be added in the system. This paper explains how to make the modulator stable with the addition of feed-forward and feed-back branches which are used to compensate the extra loop delay. Next, we restore the signal and noise transfer functions. A 4th-Order Wideband delta-sigma modulator have been designed and simulated to verify the method.