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Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells
( Zhuo Wang ),( Dan Wang ),( Yan Li ),( Xiuli Zhang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6
Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.
Zhuo Wang,Xiaoxv Gao,Wenfeng Li,Si Tan,Qiaoran Zheng 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.5
The aim of this study was to compare the phenoliccompound profiles and antioxidant capacities of eightvarieties of longan (Dimocarpus longan Lour.) planted inthe middle and upper Yangtze River area. The totalpolyphenols content (TPC) and total flavonoids content(TFC) of dried longan pulp ranged from 1.07 ± 0.05 to1.22 ± 0.05 mg gallic acid equivalents/g and 0.25 ± 0.07to 0.87 ± 0.14 mg rutin equivalents/g. UHPLC-QqQ-MS/MS analysis revealed 12 individual polyphenol compoundsin longan. The Fuwan8, Dongliang and FD97 varietiesshowed the strongest DPPH scavenging activity (IC50 of1.03 g/mL). The highest ABTS? scavenging activity wasfound in the Dongliang. In the enzyme assays, the Fuwan8and Dongliang varieties demonstrated maximum a-amylaseand a-glucosidase inhibition activities, with values of97.56 and 88.58%, respectively. The principal componentanalysis indicated that the Rongyu8 and Songfengbencultivars have nearly analogous polyphenol compounds.
Wang Wei,Liang Xuanyu,Niu Qinghe,Wang Qizhi,Zhuo Jinyi,Su Xuebin,Zhou Genmao,Zhao Lixin,Yuan Wei,Chang Jiangfang,Zheng Yongxiang,Pan Jienan,Wang Zhenzhi,Ji Zhongmin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8
It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.
Xia Wang,Changcun Wu,Jun Sun,Chuanxiang Qin,Jianjun Wang,Qiqi Zhuo,Lixing Dai 한국섬유공학회 2020 Fibers and polymers Vol.21 No.5
This study introduces a facile method to prepare syndiotactic poly(vinyl alcohol) (s-PVA) fibers containing singlewalledcarbon nanotubes (SWNTs) from the corresponding composite dispersions with tea polyphenols (TP) as dispersantunder high-speed shear flow. The formation of the composite fibrous precipitates at high shear rate is largely facilitated by theSWNTs in the dispersions and slight flow resistance. Interestingly, the obtained s-PVA/SWNTs composite precipitatespossess a three-level hierarchical structure, that is, a single fiber is assembled by fibrils which are composed of microfibrils ofs-PVA-coated SWNTs. And the s-PVA fibrous precipitates containing high amounts of SWNTs could be obtained by shearingthe dispersion with relatively low SWNTs loadings, as a result, the composite fiber containing 20.7 wt% SWNTs wasprepared from the dispersion with 10.0 wt% SWNTs. In addition, with the increase of SWNTs loadings, the amount of theprecipitates increases, but crystallinity of the precipitates decreases instead.
Huaxin Wang,Xuan Peng,Yayi Huang,Yeda Xiao,Zhuo Wang,Liying Zhan 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.12
Purpose: The aim of this study was to investigate whether propofol could attenuate hypoxia/reoxygenation-induced apoptosisand autophagy in human renal proximal tubular cells (HK-2) by inhibiting JNK activation. Materials and Methods: HK-2 cells were treated with or without propofol or JNK inhibitor SP600125 for 1 hour and then subjectedto 15 hours of hypoxia and 2 hours of reoxygenation (H/R). Cell viability and LDH release were measured with commercial kits. Cell apoptosis was evaluated by flow cytometry. The expressions of p-JNK, cleaved-caspase-3, Bcl-2, and autophagy markers LC3and p62 were measured by Western blot or immunofluorescence. Results: HK-2 cells exposed to H/R insult showed higher cell injury (detected by increased LDH release and decreased cell viability),increased cell apoptosis index and expression of cleaved-caspase-3, a decrease in the expression of Bcl-2 accompanied byincreased expression of p-JNK and LC3II, and a decrease in expression of p62. All of these alterations were attenuated by propofoltreatment. Similar effects were provoked upon treatment with the JNK inhibitor SP600125. Moreover, the protective effects weremore obvious with the combination of propofol and SP600125. Conclusion: These results suggest that propofol could attenuate hypoxia/reoxygenation induced apoptosis and autophagy inHK-2 cells, probably through inhibiting JNK activation.
Cyclooxygenase-2 Polymorphisms and Susceptibility to Colorectal Cancer: A Meta-Analysis
Jun Wang,Xufeng Guo,Jixiang Zhang,Jia Song,Mengyao Ji,Shijie Yu,Jing Wang,Zhuo Cao,Weiguo Dong 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.6
Purpose: Four polymorphisms, -765G>C, -1195G>A, 8473T>C, and Val511Ala, in the cyclooxygenase-2 (COX-2) gene were identified to be associated with colorectal cancer (CRC) risk. However, the results are inconsistent. The objective of this meta-analysis was to evaluate the association between these four polymorphisms and the risk of CRC. Materials and Methods: All eligible case-control studies published up to December 2012 on the association between the four polymorphisms of COX-2 and CRC risk were identified by searching PubMed and Web of Science. The CRC risk associated with the four polymorphisms of the COX-2 gene was estimated for each study by odds ratio (OR) together with its 95% confidence interval (CI), respectively. Results: A total of 15 case-control studies were included. Overall, no evidence has indicated that the -1195A allele, -765C allele, 8473C allele, and 511Ala allele are associated with susceptibility to CRC (-1195G>A: OR=1.11, 95% CI: 0.82-1.51, p=0.78; -765G>C: OR=1.08, 95% CI: 0.96-1.21, p=0.07; 8473T>C: OR=1.03, 95% CI: 0.89-1.18, p=0.91; Val511Ala:OR=0.71, 95% CI: 0.46-1.09, p=0.94). However, stratified analysis with ethnicity indicated that individuals with -765GC or GC/CC genotypes had an increased risk of CRC among Asian populations (GC vs. GG: OR=1.05, 95% CI: 0.87-1.28, p=0.03; GC+CC vs. GG: OR=1.08, 95% CI: 0.96-1.21, p=0.07). Conclusion: This meta-analysis indicated that -765G>C polymorphism was significantly associated with susceptibility to CRC in Asian populations.