Novel CHR-2 SINE subfamilies and t-SINEs identified in cetaceans using nonradioactive Southern blotting

Zhuo Chen,Guang Yang 한국유전학회 2010 Genes & Genomics Vol.32 No.4

The SINEs are short interspersed repetitive elements that propagate in eukaryotic genomes via transcription followed by reverse transcription. Recent genomic surveys have demonstrated that SINEs are active in shaping the architecture of genomes and therefore are powerful markers for studies of phylogeny and population biology. In the present study, a new protocol was developed to apply nonradioactive digoxigeninlabeling system into isolation of SINEs rapidly, safely, and efficiently. The application of this approach is illustrated with discovery of thirteen informative SINEs and a novel member of the t‐SINE family in the Omura’s whale (Balaenoptera omurai) and sperm whale (Physeter macrocephalus), which is the first record of t‐SINE in cetacean. Analysis of subfamilyspecific genetic variability and insertions/deletions strongly suggested the existence of some novel CHR‐2 SINE subfamilies in the cetacean genomes.

A lightweight and flexible CNT/Fe3O4 composite with high electromagnetic interference shielding performance

Zhuo Chao,Yingying Yu,Fang Lei,Dongmei Hu 한국탄소학회 2021 Carbon Letters Vol.31 No.1

Lightweight and flexible electromagnetic interference (EMI) shielding materials are in great demand for wearable EMI device. In the present work, lightweight and flexible carbon nanotube (CNT)/ferroferric oxide ( Fe3O4) composite film was made through a feasible chemical vapor deposition process for CNT film synthesis, followed by a hydrothermal reduction process for Fe3O4 coating. In the as-prepared composite, CNT film and Fe3O4 particles work as conductive skeleton and strong magnetic particle, respectively. The as-prepared composite film shows a novel EMI shielding effectiveness (SE) of 91 dB in the X-band, a small thickness of 0.09 mm and a low density of 0.86 g/cm3, which is superior to most of the carbonbased EMI materials.

Size-dependent fracture properties of cracked silicon nanofilms

Zhuo, X.R.,Beom, H.G. Elsevier 2015 Materials science & engineering. properties, micro Vol.636 No.-

<P><B>Abstract</B></P> <P>We evaluate the applicability of the critical energy release rate as a crack propagation criterion with a focus on the effect of the material size. Cracked silicon nanofilms of various thicknesses under uniaxial tensile loading are simulated to examine the influence of film thickness on the critical energy release rate. Tensile stress–strain curves in addition to the atomic configurations corresponding to different strain levels are presented to elucidate the deformation mechanism. The cracked silicon nanofilms subjected to mode I loading deform nonlinearly to some extent. The critical energy release rates are calculated by three approaches: a molecular statics simulation, the Griffith criterion, and linear elastic fracture mechanics. Results obtained from these three methods are compared with each other and the differences between them are discussed. The critical energy release rate may be capable of predicting crack propagation when the silicon nanofilms are thicker than approximately 18nm.</P>

Further-inspection for Doubly-Fed Converter: Theory, Method and Experimental Platform

Zhuo Chen,Zhengping Zhang,Zhenghang Hao,Shaohua Li,Ailing Zhang 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.6

Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells

( Zhuo Wang ),( Dan Wang ),( Yan Li ),( Xiuli Zhang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

Exploring Transferred Information for Daur Traditional Craftwork

Zhuo Bian,Liangliang Wang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.11

This paper focuses on the disappearing Chinese Daur traditional craftwork on the graphical scale to reveal the great value of Daur culture from the culture conservation perspective. Specifically, typical Daur craftwork samples are surveyed and selected as the objective for analysis, based on which, color features and interest points feature are respectively extracted employing our color card quantization method and Scale-Invariant Feature Transform technique. Moreover, in order to apply the two extracted features into practice, principle component analysis scheme is utilized to lower the complexity and improve the accuracy of the feature information. Experimental results are accomplilshed on the collected Daur craftwork dataset, which also proves the applicability and efficiency of our approach.

Atomistic investigation of crack growth resistance in a single-crystal Al-nanoplate

Zhuo, Xiao Ru,Kim, Jang Hyun,Beom, Hyeon Gyu Cambridge University Press (Materials Research Soc 2016 Journal of materials research Vol.31 No.9

<▼1><B>Abstract</B><P/></▼1><▼2><P>The fracture behavior of a single-crystal Al-nanoplate with an edge crack under tensile loading was simulated using a molecular statics technique to evaluate crack growth resistance in Al. The crack length was determined using a stiffness method. A parabolic function fitted from simulation results was used to predict the crack length from the stiffness value extracted from unloading curves. Based on energy considerations, crack growth resistance was calculated. Crack growth resistance rose sharply in the initial stages of crack growth, and with an additional crack extension, it increased gradually to converge to a constant far exceeding the fracture toughness predicted by the Griffith criterion. This trend in the crack growth resistance curve was closely related to the amorphous zone formed at the crack tip after the onset of crack propagation.</P></▼2>

Water temperature and salinity tolerance of embryos and spat of the mussel, Musculista senhousia

Zhuo Liang Liang,김영훈,임상민,강경호,Zhi-Fang Zhang 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.3

The effects of water temperature and salinity on embryonic development and spat survival of mussel Musculista senhousia were investigated. Embryos were incubated in water ranging from 0 to 35℃ and with salinity from 5‰ to 40‰. Mussel spat were tested in water from 0 to 40℃ and with salinity from 0‰ to 100‰. The optimal conditions for mussel embryos were 20-25℃ and salinity from 25‰ to 35‰, based on Within this temperature range, higher temperatures correponded to a shorter duration of the embryonic period. Optimisation of mussel spat survival was at 25-35℃ and salinity from 30‰ to 40‰; both values are higher than those for embryo, which hinted M. senhousia embryos are more vulnerable than spat. Temperatures below 15℃ were lethal for embryos, making temperature a feasible method with which to control the large population of M. senhousia in ark-shell farm during its spawning period.

Phenolic content, antioxidant capacity, and α-amylase and α-glucosidase inhibitory activities of Dimocarpus longan Lour.

Zhuo Wang,Xiaoxv Gao,Wenfeng Li,Si Tan,Qiaoran Zheng 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.5

The aim of this study was to compare the phenoliccompound profiles and antioxidant capacities of eightvarieties of longan (Dimocarpus longan Lour.) planted inthe middle and upper Yangtze River area. The totalpolyphenols content (TPC) and total flavonoids content(TFC) of dried longan pulp ranged from 1.07 ± 0.05 to1.22 ± 0.05 mg gallic acid equivalents/g and 0.25 ± 0.07to 0.87 ± 0.14 mg rutin equivalents/g. UHPLC-QqQ-MS/MS analysis revealed 12 individual polyphenol compoundsin longan. The Fuwan8, Dongliang and FD97 varietiesshowed the strongest DPPH scavenging activity (IC50 of1.03 g/mL). The highest ABTS? scavenging activity wasfound in the Dongliang. In the enzyme assays, the Fuwan8and Dongliang varieties demonstrated maximum a-amylaseand a-glucosidase inhibition activities, with values of97.56 and 88.58%, respectively. The principal componentanalysis indicated that the Rongyu8 and Songfengbencultivars have nearly analogous polyphenol compounds.

Analysis on Coexistence of Ultra Wideband with OFDM-Based Communication Systems

Zhuo Li,Weixia Zou,Bin Li,Zheng Zhou,Xiaojun Huang IEEE 2011 IEEE transactions on electromagnetic compatibility Vol.53 No.3

<P>Considering the potential interference of ultrawideband (UWB) on OFDM-based wireless communication systems, this paper studies coexistence issue between UWB with both impulse radio (IR) and multiband (MB) modulation and IEEE802.11n, WiMAX, and LTE downlink systems, using physical layer modeling strategy. According to the physical characteristics of UWB and the victim systems, entire physical layer models are set up. Based on the requirements of bit error rate (BER) and receiver sensitivity of the three victim systems, the emission limits of UWB are respectively obtained. Compared with a method based on interference to noise ratio (I/N) criteria, this method reflects the effect of the actual systems, so the conclusion can provide the reference to formulating the frequency spectrum of UWB within the operating frequency bands of the victim systems. Besides, we also reveal that IR-UWB should be more strictly regulated compared with MB-OFDM because of its time domain characteristic.</P>