KMT-2017-BLG-0165Lb: A Super-Neptune-mass Planet Orbiting a Sun-like Host Star

Kil Jung, Youn,Gould, Andrew,Zang, Weicheng,Hwang, Kyu-Ha,Ryu, Yoon-Hyun,Han, Cheongho,Yee, Jennifer C.,Albrow, Michael D.,Chung, Sun-Ju,Shin, In-Gu,Shvartzvald, Yossi,Zhu, Wei,Cha, Sang-Mok,Kim, Dong American Astronomical Society 2019 The Astronomical journal Vol.157 No.2

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Sun, Chuanxi,Zhu, Tianyi,Zhu, Yuwei,Li, Bing,Zhang, Jiaming,Liu, Yixin,Juan, Changning,Yang, Shifa,Zhao, Zengcheng,Wan, Renzhong,Lin, Shuqian,Yin, Bin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.3

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

Application Research on Mechanical Strength and Durability of Porous Basalt Concrete

Zhu, Yuelei,Li, Jingchun,Zhu, He,Jin, Long,Ren, Qifang,Ding, Yi,Li, Jinpeng,Sun, Qiqi,Wu, Zilong,Ma, Rui,Oh, Won-Chun Materials Research Society of Korea 2022 한국재료학회지 Vol.32 No.3

Porous basalt aggregate is commonly used in roadbed engineering, but its application in concrete has rarely been studied. This paper studies the application of porous basalt in concrete. Porous basalt aggregate is assessed for its effects on mechanical strength and durability of prepared C50 concrete; because it has a hole structure, porous basalt aggregate is known for its porosity, and porous basalt aggregates can be made full of water through changing the content of saturated basalt; after full-water condition is achieved in porous basalt aggregate mixture of C50 concrete, we discuss its mechanical properties and durability. The effects of C50 concrete prepared with basalt aggregate on the compressive strength, water absorption, and electric flux of concrete specimens of different ages were studied through experiments, and the effects of different replacement rates of saturated porous basalt aggregate on the properties of concrete were also studied. The results show that porous basalt aggregate can be prepared as C50 concrete. For early saturated porous basalt aggregate concrete, its compressive strength decreases with the increase of the replacement rate of saturated aggregate; this occurs up to concrete curing at 28 d, when the replacement rate of saturated basalt aggregate is greater than or equal to 40 %. The compressive strength of concrete increases with the increase of the replacement rate of saturated aggregate. The 28 d electric flux decreases with the increase of the replacement rate of saturated aggregate, indicating that saturated porous basalt aggregate can improve the chloride ion permeability resistance of concrete in later stages.

White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

Zhu Qinchao,Zhou Xuhao,Zhang Yanan,Ye Di,Yu Kang,Cao Wangbei,Zhang Liwen,Zheng Houwei,Sun Ziyang,Guo Chengchen,Hong Xiaoqian,Zhu Yang,Zhang Yajun,Xiao Ying,Valencak Teresa G.,Ren Tanchen,Ren Daxi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background : Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods : A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result : Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion : Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

Structural and component characterization of the B4C neutron conversion layer deposited by magnetron sputtering

Zhu Jingtao,Liu Yang,Zhou Jianrong,Yang Zehua,Zhu Hangyu,Zhou Xiaojuan,Tan Jinhao,Cui Mingqi,Sun Zhijia 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.9

Neutron conversion detectors that use 10B-enriched boron carbide are feasible alternatives to 3 He-based detectors. We prepared boron carbide films at micron-scale thickness using direct-current magnetron sputtering. The structural characteristics of natural B4C films, including density, roughness, crystallization, and purity, were analyzed using grazing incidence X-ray reflectivity, X-ray diffraction, X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectrometry, and scanning electron microscopy. A beam profile test was conducted to verify the practicality of the 10B-enriched B4C neutron conversion layer. A clear profile indicated the high quality of the neutron conversion of the boron carbide layer.

IL-12-STAT4-IFN-γ axis is a key downstream pathway in the development of IL-13-mediated asthma phenotypes in a Th2 type asthma model

You-Sun Kim,Seng-Jin Choi,Jun-Pyo Choi,전성규,이병재,고용송,이춘근,Jack A. Elias,Yoon-Keun Kim,Sun-Young Oh,Zhou Zhu 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.8

IL-4 and IL-13 are closely related cytokines that are produced by Th2 cells. However, IL-4 and IL-13 have different effects on the development of asthma phenotypes. Here, we evaluated downstream molecular mechanisms involved in the development of Th2 type asthma phenotypes. A murine model of Th2 asthma was used that involved intraperitoneal sensitization with an allergen (ovalbumin) plus alum and then challenge with ovalbumin alone. Asthma phenotypes, including airway-hyperresponsiveness (AHR), lung inflammation,and immunologic parameters were evaluated after allergen challenge in mice deficient in candidate genes. The present study showed that methacholine AHR and lung inflammation developed in allergen-challenged IL-4-deficient mice but not in allergen-challenged IL-13-deficient mice. In addition, the production of OVA-specific IgG2a and IFN-γ-inducible protein (IP)-10was also impaired in the absence of IL-13, but not of IL-4. Lung-targeted IFN-γ over-expression in the airways enhanced methacholine AHR and non-eosinophilic inflammation; in addition, these asthma phenotypes were impaired in allergen-challenged IFN-γ-deficient mice. Moreover, AHR, non-eosinophilic inflammation,and IFN-γ expression were impaired in allergen-challenged IL-12Rβ2- and STAT4-deficient mice; however, AHR and non-eosinophilic inflammation were not impaired in allergen-challenged IL-4Rα-deficient mice, and these phenomena were accompanied by the enhanced expression of IL-12 and IFN-γ. The present data suggest that IL-13-mediated asthma phenotypes, such as AHR and non-eosinophilic inflammation, in the Th2 type asthma are dependent on the IL-12-STAT4-IFN-γ axis, and that these asthma phenotypes are independent of IL-4Ralpha-mediated signaling.

Improved Mechanical Properties of Friction Stir Welded 1500 MPa Martensitic Steel Joints with Additional Water Cooling

Shuhao Zhu,Pengcheng Zhu,Binbin Wang,Liang Zhang,Yufeng Sun,Shaokang Guan 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1

This study proposes a method to improve the mechanical strength of martensitic welded joints by minimizing the effect ofmartensitic tempering. The high-strength martensitic steel plate exhibiting a tensile strength of 1500 MPa was successfullyjoined in this study utilizing additional water-cooling by friction stir welding (FSW) technique. Concurrently, conventionalair-cooled welded joints were produced utilizing identical welding parameters for comparison. Notwithstanding the macrostructureof both FSW joints was similar, the air-cooled joint contained a larger stir zone (SZ) and wider heat-affectedzone (HAZ). The air-cooled joint SZ exhibited a microstructure featuring coarse martensite, while the water-cooled jointSZ displayed a microstructure consisting of fine martensite. The degree of self-tempering of the martensite in the HAZ wassignificantly reduced, together with fewer and finer carbide particles than in the HAZ of air-cooled joints, by applying watercooling. The tensile test results indicated that the water-cooled FSW joint showed a significant improvement (23.6%) inultimate tensile strength compared to its air-cooled counterparts.

Parameter Sensitivity Study on Static and Dynamic Mechanical Properties of the Spatial Y-shaped Tied Arch Bridge

Jianpeng Sun,Zihan Tan,Jiaju Zhang,Wenwu Sun,Li Zhu 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.2

The spatial Y-shaped tied arch bridge is a rare form of innovative bridge on arch bridges around the world. It has important reference significance for the design and construction of bridge engineering worldwide. This arch bridge is novel in design and adopts single and double arch ribs combined structure. However, as a novel bridge type, its force situation is indetermination, so it is very important to study its mechanical properties and parameter sensitivity. In order to study the mechanical properties of the spatial Y-shaped tied arch bridge and the influence of structural parameters, this paper takes a spatial Y-shaped tied arch bridge under construction in China as the research object. The finite element software MIDAS Civil is used to establish the bridge model. The finite element model is used to analyze the static and dynamic performance of the spatial Y-shaped tied arch bridge under the use stage and the mechanical change trend under the influence of different rise-span ratios and double arch bifurcation angles. The results of single arch rib and double arch rib under constant load and live load are compared under different structural parameters in this paper, and the parameter sensitivity analysis of statics and dynamics is carried out. These analyses provide the adjustment basis and design reference for the design of the special-shaped arch bridge in the future.

Screening nephroprotective compounds from cortex Moutan by mesangial cell extraction and UPLC

Min Sun,Limei Huang,Jianliang Zhu,Wenjie Bu,Jing Sun,Zhaohui Fang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

A method for screening nephroprotective compoundsin cortex Moutan, a common traditional Chinesemedicine (TCM) in treating diabetic nephropathy with renalmesangial cell extraction and ultra performance liquidchromatography technique was described in this paper. Wehypothesize that the compounds which bind to cell membranesunder pathological conditions may be the bioactivecompounds in TCMs. Mesangial cells were cultured inmedium containing 5 mM (physiological, NG) or 30 mM(pathological, HG) glucose for 48 h and then incubated withcortex Moutan extract. After the unbound substances werewashed off, the cell membrane-bound compounds weredissociated and concentrated by an SPE column. By comparingthe chromatograms of NG and HG cultured-cellextractions and cortex Moutan extract, three compoundsbound to both NG and HG-cultured mesangial cells wereidentified as paeoniflorin, pentagalloylglucose (PGG) andpaeonol. In vitro studies showed that paeoniflorin, PGG andpaeonol reduced the activity of nicotinamide-adenine dinucleotidephosphate oxidase (NADPH) activity, anddecreased the level of reactive oxygen species (ROS),transforming growth factor-b1 (TGF-b1) and fibronectin inhigh glucose cultured mesangial cells. The results indicatethat paeonol, paeoniflorin and PGG may be the nephroprotectivecompounds from cortex Moutan. This study isexpected to provide a more reliable and effective method forscreening bioactive compounds from the complex TCM systems.

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Chuanxi Sun,Tianyi Zhu,Yuwei Zhu,Bing Li,Jiaming Zhang,Yixin Liu,Changning Juan,Shifa Yang,Zengcheng Zhao,Renzhong Wan,Shuqian Lin,Bin Yin 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.4

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10 mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were “protein processing in endoplasmic reticulum,” “retinol metabolism,” and “glycine, serine, and threonine metabolism.” Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.