Mapping the Mangrove Forest Restoration Potential and Conservation Gaps in China Based on Random Forest Model

Zhonghua Yu,Wei Li,Shaowei Zhang,Buqing Zhong,Jun Wang,이시영,최재혁,Shulin Deng 인간식물환경학회 2022 인간식물환경학회지 Vol.25 No.4

Background and objective: The area of mangroves is gradually decreasing globally, and mangroves are already one of the most threatened ecosystems. Despite net growth in the mangrove areas in China, the restoration potential of mangroves is still insufficient. This study proposed the Random forest model as an excellent data mining method to map the restoration potential based on the predicted probability of mangrove habitat suitability. Methods: We demonstrated the vital environmental variables influencing habitat suitability. The de-cisive advantages of RFM were parsimonious (variables selection), cost-effective (us-ing existing open-source data), accurate (training AUC was 0.89, testing AUC was 0.91), highly efficient (fast-training speed); and its results had high explanatory power. Here, we first mapped the conservation gaps using the RFM. Results: The results showed that temperature was the most important environmental factor influencing the habitat suit-ability of mangroves. The northern limit of suitable areas was around 24°44' N. The theoretical suitable habitat area for mangrove was 196,566.6 ha (the highly suitable area was 32,551.4 ha, the medium suitable area was 164,015.2 ha). The potential area for mangrove restoration was 176,264 ha (Guangdong with 104215.4 ha, Guangxi with 65957.5 ha). Conclusion: We proposed 24 sites with conservation gaps for mangrove forests restoration and nine potential sites as examples for the further restoration plan. We took one example site with high restoration potential for further explanation: how the key environmental factors influence the habitat suitability and how to use the infor-mation to guide the restoration strategies. RFM can be used as a data mining algo-rithm for the utmost use of the presence-only ecological data, objectively evaluating the suitability of species distribution, and providing scientifically technical data for species restoration planning.

Study on the Embedded SPC System Bused on LAN

Yin, Jianjun,Yu, Zhonghua,Wu, Zhaotong The Korean Society for Quality Management 2003 The Asian Journal on Quality Vol.4 No.1

Statistical Process Control (SPC) is recognized as one of the most important tool of quality control (QC), and is one research focus in order to implement SPC quickly and effectively using computer technology and network environments. This paper proposes the principle and topology of embedded SPC based on the Internet/lntranet, emphatically discusses the embedded data sampling block, embedded network interface and SPC strategy. An example of application is given.

Study on the Embedded SPC System Based on LAN

Jun, Yinjian,Yu, Zhonghua,Wu, Zhaotong 한국품질경영학회 2003 The Asian Journal on Quality Vol.4 No.1

Statistical Process Control (SPC) is recognized as one of the most important tool of quality control (QC), and is one research focus in order to implement SPC quickly and effectively using computer technology and network environments. This paper proposes the principle and topology of embedded SPC based on the Internet/Intranet, emphatically discusses the embedded data sampling block, embedded network interface and SPC strategy. An example of application is given.

Synthesis and characterization of Ba(TI1-xZRx)O₃ nanopowders by an aqueous coprecipitation method

Xiangbang Cheng,Zhuo Li,Zhonghua Yao,Yang Liu,Zhiyong Yu,Minghe Cao,Hanxing Liu 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.6

In this study, single phase Ba(Ti1-xZrx)O3 (0 ≤ × ≤ 0.15) nanopowders have been synthesized successfully by an aqueous coprecipitation method at 85 oC without any further heat treatment. The lattice strain calculated by the Hall-Williamson method indicates that the broadening of the XRD peaks is primarily due to the size of particles not to the lattice strain. The increase of the lattice parameter indicates that Zr ions have entered the lattice and a solid solution was successfully obtained. Furthermore, the particle sizes calculated by the Sherrer formula from the XRD patterns are in agreement with the results of SEM and TEM images. The nanopowders obtained can be used to prepare Ba(Ti1-xZrx)O3 ceramics with a tailored grain size and homogeneous composition, and this is significant to investigate the size effect in Ba(Ti1-xZrx)O3 ceramics and understand the relaxor behavior thoroughly. In this study, single phase Ba(Ti1-xZrx)O3 (0 ≤ × ≤ 0.15) nanopowders have been synthesized successfully by an aqueous coprecipitation method at 85 oC without any further heat treatment. The lattice strain calculated by the Hall-Williamson method indicates that the broadening of the XRD peaks is primarily due to the size of particles not to the lattice strain. The increase of the lattice parameter indicates that Zr ions have entered the lattice and a solid solution was successfully obtained. Furthermore, the particle sizes calculated by the Sherrer formula from the XRD patterns are in agreement with the results of SEM and TEM images. The nanopowders obtained can be used to prepare Ba(Ti1-xZrx)O3 ceramics with a tailored grain size and homogeneous composition, and this is significant to investigate the size effect in Ba(Ti1-xZrx)O3 ceramics and understand the relaxor behavior thoroughly.

Derivation of endothelial cells from porcine induced pluripotent stem cells by optimized single layer culture system

Renyue Wei,Jiawei Lv,Xuechun Li,Yan Li,Qianqian Xu,Junxue Jin,Yu Zhang,Zhonghua Liu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.1

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells

Jiawei Lv,Shuang Wu,Renyue Wei,Yan Li,Junxue Jin,Yanshuang Mu,Yu Zhang,Qingran Kong,Xiaogang Weng,Zhonghua Liu 대한수의학회 2019 Journal of Veterinary Science Vol.20 No.3

The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly used in application of CRISPR/Cas9; however, the relationship between the length of the guide sequence and the efficiency of CRISPR/ Cas9 in porcine cells is still not clear. To illustrate this issue, guide RNAs of different lengths targeting the EGFP gene were designed. Specifically, guide RNAs of 17 nt or longer were sufficient to direct the Cas9 protein to cleave target DNA sequences, while 15 nt or shorter guide RNAs had loss-of-function. Full-length guide RNAs complemented with mismatches also showed loss-of-function. When the shortened guide RNA and target DNA heteroduplex (gRNA:DNA heteroduplex) was blocked by mismatch, the CRISPR/Cas9 would be interfered with. These results suggested the length of the gRNA:DNA heteroduplex was a key factor for maintaining high efficiency of the CRISPR/Cas9 system rather than weak bonding between shortened guide RNA and Cas9 in porcine cells.