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        Ds9 was isolated encoding as OsHAP3H and its C-terminus was required for interaction with HAP2 and HAP5

        Qikai Xing,Zhimin Zheng,Xiangui Zhou,Xujun Chen,Zejian Guo 한국식물학회 2015 Journal of Plant Biology Vol.58 No.1

        Heading date is one of the most important agronomic traits in rice. Here, we analyzed an early flowering mutant ds9 under long day (LD) conditions and found that the early flowering phenotype was controlled by a single dominant gene. Mapping the Ds9 revealed that it was located near SSR marker RM5432 on chromosome 8, close to the known heading date-related DTH8/Ghd8 locus. Sequence analysis indicated that Ds9 was allelic to Ghd8, encoding the OsHAP3H subunit of CCAAT-box binding proteins (HAPs), and transformation of ds9 mutant with the OsHAP3H gene from wild type XS11 plants significantly increased the heading date under LD conditions. Yeast two hybrid and three hybrid assays revealed that the C-terminus of OsHAP3H is required for its interactions with OsHAP2 and OsHAP5. The data demonstrated that OsHAP3H plays a critical role as a repressor of photoperiodic flowering in rice.

      • KCI등재후보

        Evaluation of Clostridium autoethanogenum protein as a new protein source for broiler chickens in replacement of soybean meal

        Chen Xing,Zheng Aijuan,Shoaib Ahmed Pirzado,Chen Zhimin,Qiu Kai,Wang Zedong,Chang Wenhuan,Cai Huiyi,Liu Guohua 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.7

        Objective: The object of this study was to investigate the effect of replacing soybean meal with Clostridium autoethanogenum protein (CAP) in broiler diets on growth performance, blood indicators, antioxidant capacity, and immune function. Methods: A total of 180 Arbor Acres broilers were randomly divided into three treatments, each treatment with six replicates and 10 broilers per replicate for a 42-day feeding trial. The control group (CON) was fed corn-soybean meal based diet. The CAP-1 and CAP-2 groups were considered to use CAP to replace 25% or 50% of soybean meal in the diet, respectively. The average daily gain and average daily feed intake of broilers at 1 to 21 d, 22 to 42 d, and 1 to 42 d were measured, and the feed conversion ratio was calculated. At the 42nd day of age, two broilers with similar weights and fasted for 12 h were selected in each replicate for blood collection from the brachial wing vein. The blood routine indicators, serum biochemical indicators, serum antioxidant capacity, and immunoglobulin content of broiler chickens were measured. Results: Replacement of soybean meal with 25% (CAP-1) and 50% (CAP-2) CAP significantly increased the average daily gain of 22 to 42 d and 1 to 42 d and decreased the average daily feed intake and feed conversion rate (p<0.05). The CAP-1 group, and CAP-2 group significantly increased hemoglobulin in the blood of broilers, while the CAP-2 group increased hematocrit content (p<0.05). Compared with the control group, the contents of superoxide dismutase and immunoglobulin A in serum of the CAP-2 group were significantly increased, while the contents of malondialdehyde in CAP group were significantly decreased (p<0.05). Conclusion: Replacing soybean meal with CAP led to significant improvements in the growth performance, antioxidant capacity, and immunoglobulin content of broilers. Objective: The object of this study was to investigate the effect of replacing soybean meal with <i>Clostridium autoethanogenum</i> protein (CAP) in broiler diets on growth performance, blood indicators, antioxidant capacity, and immune function.Methods: A total of 180 Arbor Acres broilers were randomly divided into three treatments, each treatment with six replicates and 10 broilers per replicate for a 42-day feeding trial. The control group (CON) was fed corn-soybean meal based diet. The CAP-1 and CAP-2 groups were considered to use CAP to replace 25% or 50% of soybean meal in the diet, respectively. The average daily gain and average daily feed intake of broilers at 1 to 21 d, 22 to 42 d, and 1 to 42 d were measured, and the feed conversion ratio was calculated. At the 42nd day of age, two broilers with similar weights and fasted for 12 h were selected in each replicate for blood collection from the brachial wing vein. The blood routine indicators, serum biochemical indicators, serum antioxidant capacity, and immunoglobulin content of broiler chickens were measured.Results: Replacement of soybean meal with 25% (CAP-1) and 50% (CAP-2) CAP significantly increased the average daily gain of 22 to 42 d and 1 to 42 d and decreased the average daily feed intake and feed conversion rate (p<0.05). The CAP-1 group, and CAP-2 group significantly increased hemoglobulin in the blood of broilers, while the CAP-2 group increased hematocrit content (p<0.05). Compared with the control group, the contents of superoxide dismutase and immunoglobulin A in serum of the CAP-2 group were significantly increased, while the contents of malondialdehyde in CAP group were significantly decreased (p<0.05).Conclusion: Replacing soybean meal with CAP led to significant improvements in the growth performance, antioxidant capacity, and immunoglobulin content of broilers.

      • KCI등재

        MoO3 modified CeO2/TiO2 catalyst prepared by a single step sol–gel method for selective catalytic reduction of NO with NH3

        Ye Jiang,Zhimin Xing,Xuechong Wang,Shanbo Huang,Qingyu Liu,Jingshan Yang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.29 No.-

        A MoO3 modified CeO2/TiO2 catalyst was prepared by a single step sol–gel method for selective catalyticreduction of NO with NH3. The experimental results indicates that MoO3 modified CeO2/TiO2 catalystexhibited higher SCR activity and resistance to 10% H2O and 1000 ppm SO2. From the characterizationresults, it can be concluded that good physical properties, highly dispersed active species of Ce and Mo,more appearance of Ce3+ and chemisorbed oxygen, strong interaction among ceria, molybdenum andtitania, and high oxygen storage capacity might contribute to the excellent deNOx performance of CeO2–MoO3/TiO2 catalyst prepared by a single step sol–gel method.

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