Paeoniflorin reduces the inflammatory response of THP-1 cells by up‐regulating microRNA-124

Danyun Huang,Zhijun Li,Yue Chen,Yan Fan,Tao Yu 한국유전학회 2021 Genes & Genomics Vol.43 No.6

Background The activation of macrophages and the release of infammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of infammation and immunity. Objective To explore whether paeoniforin (PF) regulates the biological functions of macrophages depends on miR-124. Methods RT-PCR, WB, ELISA, CCK-8 and fow cytometry were used to evaluate that PF regulated the biological functions of THP-1 cells through miR-124. Results PF signifcantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1βin THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS+mimic-NC group, LPS+miR-124 mimic signifcantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS+PF+inhibitor-NC group, LPS+PF+miR-124 inhibitor signifcantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells. Conclusions By down-regulating miR-124, PF inhibits the proliferation and infammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.

Nonlinear stability of the upper chords in half-through truss bridges

Wen Qingjie,Yue Zixiang,Liu Zhijun 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.36 No.3

The upper chords in half-through truss bridges are prone to buckling due to a lack of the upper transverse connections. Taking into account geometric and material nonlinearity, nonlinear finite-element analysis of a simple supported truss bridge was carried out to exhibit effects of different types of initial imperfections. A half-wave of initial imperfection was proved to be effective in the nonlinear buckling analysis. And a parameter analysis of initial imperfections was also conducted to reveal that the upper chords have the greatest impact on the buckling, followed by the bottom chords, vertical and diagonal web members. Yet initial imperfections of transverse beams have almost no effect on the buckling. Moreover, using influence surface method, the combinatorial effects of initial imperfections were compared to demonstrate that initial imperfections of the upper chords play a leading role. Furthermore, the equivalent effective length coefficients of the upper chord were derived to be 0.2~ 0.28 by different methods, which implies vertical and diagonal web members still provide effective constraints for the upper chord despite a lack of the upper transverse connections between the two upper chords. Therefore, the geometrical and material nonlinear finite-element method is effective in the buckling analysis due to its higher precision. Based on nonlinear analysis and installation deviations of members, initial imperfection of l/500 is recommended in the nonlinear analysis of half-through truss bridges without initial imperfection investigation.

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times

Miao Zhang,Zhenhua Yue,Zhijun Liu,Ali Islam,Buriro Rehana,Shu Tang,Jörg Hartung,Endong Bao 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.3

The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation.

Modeling and Neuro-Fuzzy Adaptive Attitude Control for Eight-Rotor MAV

Xiangjian Chen,Di Li,Yue Bai,Zhijun Xu 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.6

This paper focuses on modeling and intelligent control of the new Eight-Rotor MAV which is used to solve the problem of low coefficient proportion between lift and gravity for Quadrotor MAV. The dynamical and kinematical modeling for the Eight-Rotor MAV was developed which has never been proposed before. Based on the achieved dynamic modeling, two types of controller were presented. One type, a PID controller is derived in a conventional way with simplified dynamics and turns out to be quite sensitive to sensor noise as well as external perturbation. The second type controller is the Neuro-Fuzzy adaptive controller which is composed of two type-II fuzzy neural networks (T-IIFNNs) and one PD controller: The PD controller is adopted to control the attitude, one of the T-IIFNNs is designed to learn the inverse model of Eight-Rotor MAV on-line, the other one is the copy of the former one to compensate for model errors and external disturbances, both structure and parameters of T-IIFNNs are tuned on-line at the same time, and then the stability of the Eight-Rotor MAV closed-loop control system is proved using Lyapunov stability theory. Finally, the validity of the proposed control method has been verified through real-time experiments. The experimental results show that the performance of Neuro-Fuzzy adaptive controller performs very well under sensor noise and external disturbances, and has more superiority than traditional PID controller.

Microstructure and Electromagnetic Properties of Y3+ Substituted Mg-Mn Nanocrystalline Ferrites Via Hydrothermal Procedure

Renxin Xu,Huan Yang,Huajun Sun,Jing Zhou,Zhijun Yue,Xinhua Gao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2

Mg0.2Mn0.8YxFe2-xO4 (x = 0.000, 0.025, 0.050 and 0.075) nanocrystalline is synthesized via hydrothermal technique. Single cubic spinel phase is confirmed by XRD and the formation of Mg0.2Mn0.8YxFe2-xO4 is verified by EDS. The average size of the nanoferrites is around 80 nm, which is examined by TEM image. Y3+ substituted Mg-Mn nanoferrites exhibit low saturation magnetization (Ms) and coercivity (Hc). Especially, the nanoferrites with x = 0.075 show the lowest Ms and Hc of 46.96 emu/g and 12.04 Oe, respectively. The ferrite composites present that the resistivity and the magnetic loss are less than 4.5 x 10 7 Ω · cm and 0.7 in the range of 2–18 GHz, respectively. The low coercivity and high resistivity indicate that the addition of Y3+ contributes to the synthesis of the excellent soft magnetic materials.

Low beta superconducting cavity system design for HIAF iLinac

Mengxin Xu,Yuan He,Shengxue Zhang,Lubei Liu,Tiancai Jiang,Zehua Liang,Tong Liu,Yue Tao,Chunlong Li,Qitong Huang,Fengfeng Wang,Hao Guo,Feng Bai,Xianbo Xu,Shichun Huang,Xiaoli Li,Zhijun Wang,Shenghu Zha Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.7

A superconducting ion-Linac (iLinac), which is supposed to work as the injector in the High Intensity heavy-ion Accelerator Facility project, is under development at the Institute of Modern Physics (IMP), Chinese Academy of Sciences. The iLinac is a superconducting heavy ion linear accelerator approximately 100 meters long and contains 96 superconducting cavities in two types of 17 cyromodules. Two types of superconducting resonators (quarter-wave resonators with a frequency of 81.25 MHz and an optimal beta β = v/c = 0.07 called QWR007 and half-wave resonators with a frequency of 162.5 MHz and an optimal beta β = 0.15 called HWR015) have been investigated. The cavity design included extensive multi-parameter electromagnetic simulations and mechanical analysis, and its results are described in details. The fundamental power coupler and cavity dynamic tuner designs are also presented in this article. The prototypes are under manufacturing and expected to be ready in 2023.