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Investigation of Some Hard Coatings Synthesized by Ion Beam Assisted Deposition
He, Jian-Li,Li, Wen-Zhi,He, Xial-Ming,Liu, Chang-Hong The Korean Vacuum Society 1995 Applied Science and Convergence Technology Vol.4 No.s2
Ion beam assisted deposition(IBAD) technique was used to synthesize hard coatings including diamond-like carbon(DLC), carbon nitride(CN) and metal-ceramic multilayered films. It was found that DLC films formed at low energy ion bombardment possess more $Sp^3$ bonds and much higher hardness. The films exhibited an excellent wear resistance. Nanometer multialyered Fe/TiC films was deposited by ion beam sputtering. The structure and properties were strongly dependent on the thickness of the individual layers and modulation wave length. It was disclosed that both hardness and toughness of the films could be enhanced by adjusting the deposition parameters. The CN films synthesized by IBAD method consisted of tiny crystallites dispersed in amorphous matrix, which were identified by electron diffraction pattern to be $\beta -C_3N_4$.
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Research on the Oxidation-Protective Coatings for Carbon/Carbon Composites
He-Jun Li,Qian-Gang Fu,Jian-Feng Huang,Xie-Rong Zeng,Ke-Zhi Li 한국탄소학회 2005 Carbon Letters Vol.6 No.2
Anti-oxidation coatings are the key technique for carbon/carbon (C/C) composites used as the thermal structural materials. The microstructure and oxidation behavior of several kinds of high-performance ceramic coatings for C/C composites prepared in Northwestern Polytechnical University were introduced in this paper. It showed that the ceramic coatings such as SiC, Si-MoSi2, SiC-MoSi2, Al2O3-mullite-SiC and SiC/yttrium silicate/glass coatings possessed excellent oxidation resistance at high temperatures, and some of these coatings were characterized with excellent thermal shock resistance. The SiC-MoSi2 coating system has the best oxidation protective property, which can effectively protect C/C composites from oxidation up to 1973 K. In addition, the protection and failure reasons of some coatings at high temperature were also provided.
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Zhi Yuan Fu,Hui Ling Xie,Jian Sheng Li,Yan Min Hu,Zong Hua Liu,Zhong You He,Ji Hua Tang 한국유전학회 2008 Genes & Genomics Vol.30 No.6
Thermo-sensitive genic male sterile (TGMS) lines can provide new options for hybrid seed production using "two-line" system. A set of F2 and BC1 populations derived from the cross between Qiong-6ms and Dan958 were employed to analyze the inheritance of a TGMS line Qiong-6ms and map the TGMS genes in maize. The results demonstrated that the sterility of Qiong-6ms was governed by two duplicative recessive genes, named tms1 and tms2. The gene tms1 was mapped to chromosome 5 linked with the SSR markers umc1355, umc2302 and umc1784 at a distance of 3.0 cM, 1.3 cM and 0.9 cM respectively; while tms2 was localized on chromosome 3, linked with SSR markers bnlg1605 (0.5 cM) and umc2050 (4.2 cM). These markers, which are tightly linked with the tms1 and tms2 genes, will be helpful for marker assisted selection of TGMS lines in maize.
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Prognostic Significance of Overexpression of EZH2 and H3k27me3 Proteins in Gastric Cancer
He, Long-Jun,Cai, Mu-Yan,Xu, Guo-Liang,Li, Jian-Jun,Weng, Zi-Jin,Xu, Da-Zhi,Luo, Guang-Yu,Zhu, Sen-Lin,Xie, Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7
The enhancer of zeste homolog 2 (EZH2) methyl transferase and histone 3 lysine 27 (H3K27me3) protein can repress gene transcription, and their aberrant expression has been observed in various human cancers. This study determined their expression levels in gastric cancer tissues with reference to clinicopathological features and patient survival. We collected 117 gastric cancer and corresponding normal tissues for immunohistochemistry analysis. In gastric cancers, 82/117 (70.1%) were positive for EZH2 and 66/117 (56.4%) for H3K27me3 proteins in contrast to only 5.41% and 7.25% of normal gastric mucosa specimens, respectively. Kaplan-Meier survival data showed the average overall and disease-free survival of EZH2 high expression patients was 25.2 and 20.2 months, respectively, shorter than that with EZH2 low expression (40.5 and 35.9 months). The average overall survival and disease-free survival of high H3K27me3 expression patients was 23.4 and 17.4 months, shorter than without H3K27me3 expression (37.6 and 34.5 months). The average overall survival and disease-free survival of patients with both EZH2 and H3K27me3 expression was 18.8 and 12.9 months, respectively, shorter than that with either alone (34.7 and 31.2 months) or with low levels of both (43.9 and 39.9 months). Multivariate Cox regression analysis showed that H3K27me3 and EZH2 expression, tumor size differentiation and clinical stage were all independent prognostic factors for predicting patient survival. This study demonstrated that detection of both EZH2 and H3K27me3 proteins can predict poor survival of gastric cancer patients, superior to single protein detection. In addition, H3K27me3 and EZH2 protein expression could predict lymph node metastasis.
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Wang, Jian,Yang, Jing,Li, Bin-Bin,He, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: This study aimed at investigating whether the orphan nuclear receptor NR4A2 is significantly associated with clinicopathologic features and overall survival of patients with nasopharyngeal carcinoma (NPC). Methods: Immunohistochemistry was performed to determine NR4A2 protein expression in 84 NPC tissues and 20 non-cancerous nasopharyngeal (NP) tissues. The prognostic significance of NR4A2 protein expression was evaluated using Cox proportional hazards regression models and Kaplan-Meier survival analysis. Results: We did not find a significant association between total NR4A2 expression and clinicopathological variables in 84 patients with NPC. However, we observed that high cytoplasmic expression of NR4A2 was significantly associated with tumor size (T classification) (P = 0.006), lymph node metastasis (N classification) (P = 0.002) and clinical stage (P = 0.017). Patients with higher cytoplasmic NR4A2 expression had a significantly lower survival rate than those with lower cytoplasmic NR4A2 expression (P = 0.004). Multivariate Cox regression analysis analysis suggested that the level of cytoplasmic NR4A2 expression was an independent prognostic indicator for overall survival of patients with NPC (P = 0.033). Conclusions: High cytoplasmic expression of NR4A2 is a potential unfavorable prognostic factor for patients with NPC.
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Orphan Nuclear Receptor Nurr1 as a Potential Novel Marker for Progression in Human Prostate Cancer
Wang, Jian,Yang, Jing,Zou, Ying,Huang, Guo-Liang,He, Zhi-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
A number of studies have indicated that Nurr1, which belongs to a novel class of orphan nuclear receptors (the NR4A family), is important for carcinogenesis. Here we investigated expression of Nurr1 protein in benign and malignant human prostate tissues and association with clinicopathologic features using immunohistochemical techniques. Moreover, we also investigated the ability of Nurr1 to influence proliferation, migration, invasion and apoptosis of human prostate cancer cells using small interfering RNA silencing. Immunohistochemical analysis revealed that the expression of Nurr1 protein was higher in prostate cancer tissues than in benign prostate tissue (P<0.001), levels being positively correlated with tumor T classification (P = 0.003), N classification (P = 0.017), M classification (P = 0.011) and the Gleason score (P = 0.020) of prostate cancer patients. In vitro, silencing of endogenous Nurr1 attenuated cell proliferation, migration and invasion, and induced apoptosis of prostate cancer cells. These results suggest that Nurr1 may be used as an indicator for prostate cancer progression and be useful for novel potential therapeutic strategies.
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( Guo Jian He ),( Zhi Hong Bao ),( Tian Yu Liu ),( Shuai Li ),( Jeong Ryong Chae ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1
Purpose: The purpose of this study was designed to examine the activation of Fatigue Substance, Oxidative Stress and Antioxidant Enzyme in Wushu Sanda players according to the different exercise intensity. Methods & Materials: Subjects of this four-week-long Wushu exercise program were divided into 2 groups: high intensity group (n=8), and low intensity group (n=8). Subjects participated in this program once a day (high intensity group:60mins, low intensity group:90mins) and 5 times per week (Monday to Friday). Every subjects’ fatigue substance (LAC, LDH), oxidative stress (MDA) and antioxidant enzyme (GPx, SOD, CAT) were measured before the program, immediately after exercise, after 30mins recovery and after 60mins recovery. All these parameters were measured again four-week latter. Statistics: ANOVA, paired t-test and repeated ANOVA were carried out to analyze the changes before and after the exercises, changes in terms of fatigue substance, oxidative stress and antioxidant enzyme, respectively. Statistical significance is established at the .05 level (p<.05). Result: 1. Change of LAC and LDH Pre and Post exercises, analysis of the LAC have statistically significant changes in recovery 30 and 60minutes than immediately after within each group (p<.05). But according to the time of measurement there are no statistically significant difference (p>.05). And, after four weeks, LAC in both groups are lower than the concentration of before exercise. Especially, the low intensity exercise group are lower than the high intensity exercise group. But all variables are no statistically significant differences(p<.05). Pre and Post exercises, analysis of the LDH was statistically significant changes in immediately after and recovery 60minutes within each group(p<.05). And according to the time of measurement were only significantly difference in the rest (p<.05). What’s more, the high intensity exercise group are lower than the low intensity exercise group (p>.05). 2. Change of MDA MDA in low intensity group was significantly higher after exercise and 30mins recovery than before exercise (p<.05). In high intensity group, no significant difference was found. 3. Change of GPx, SOD, CAT After the four weeks exercise, GPx in high intensity group shows significant differences after 30mins and 60mins recovery compared with the value of pre-exercise (p<.05). No significant difference was found in low intensity group. Compared to the value of SOD pre-exercise, the SOD of post-exercise shows significant differences after 30mins and 60mins recovery in high intensity group ( p<.05) while only immediately after exercise in low intensity group (p<.01 ). In CAT, a significant difference was found in high intensity group after the four weeks exercise (p<.05). No significant difference was found in low intensity group. Conclusion: After four weeks exercise, some differences were found in fatigue substance(LAC, LDH), oxidative stress(MDA) and antioxidant enzyme(GPx, SOD, CAT) in both groups: LDH was found to be increased in high intensity group after the four-week exercise; MDA was increased in low intensity group after exercise and 30mins recovery than before exercise; compared with the value of pre-exercise, GPx and SOD in high intensity group shows significant differences after 30mins and 60mins recovery; while in low intensity group, the SOD difference only occurred immediately after exercise; In CAT, a significant difference was found in high intensity group but no significant difference was found in low intensity group.
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Research on the Oxidation-Protective Coatings for Carbon/Carbon Composites
Li, He-Jun,Fu, Qian-Gang,Huang, Jian-Feng,Zeng, Xie-Rong,Li, Ke-Zhi Korean Carbon Society 2005 Carbon Letters Vol.6 No.2
Anti-oxidation coatings are the key technique for carbon/carbon (C/C) composites used as the thermal structural materials. The microstructure and oxidation behavior of several kinds of high-performance ceramic coatings for C/C composites prepared in Northwestern Polytechnical University were introduced in this paper. It showed that the ceramic coatings such as SiC, Si-$MoSi_2$, SiC-$MoSi_2$, $Al_2O_3$-mullite-SiC and SiC/yttrium silicate/glass coatings possessed excellent oxidation resistance at high temperatures, and some of these coatings were characterized with excellent thermal shock resistance. The SiC-$MoSi_2$ coating system has the best oxidation protective property, which can effectively protect C/C composites from oxidation up to 1973 K. In addition, the protection and failure reasons of some coatings at high temperature were also provided.
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