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- 저자 : Zhi Junrui (검색결과 2 건)
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Zhang Tao,Zhi Junrui,Liu Yong,Yue Wenbo,Zeng Guang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Calcium ion (Ca 2+ ) plays an important role in plant–insect interactions. Ethylene glycol-bis(2-aminoethylether)-N, N, N ′ , N ′ -tetraacetic acid (EGTA), a Ca 2+ chelator, can abolish Ca 2+ in plant cells following the disruption of plant physiological processes and the plant’s defense responses against biotic stress. To clarify whether EGTA pretreatment plants have an inhibitory effect on insect feeding induction, we determined variations in kidney bean (Phaseolus vulgaris (L., Fabales: Fabaceae)) plant defense responses induced by western flower thrips (WFT) (Frankliniella occidentalis (Pergande)) feeding and the reversal effects of EGTA. The results showed that WFT feeding can significantly activate the expression level of the jasmonic acid (JA) pathway marker genes allene oxide synthase (AOS) and lipoxygenase (LOX) and salicylic acid (SA) pathway marker genes phenylalanine ammonia lyase (PAL) and β-1,3 glucanase (PR-2), and induce the biosynthesis of JA and SA. However, pre treatment with EGTA showed varying degrees of reversal effects on JA and SA content and related gene expression levels with WFT feeding. These results indicate that WFT feeding can induce the biosynthesis of JA and SA and the expression of related genes to enhance the defense responses in kidney beans plants, while pretreatment with EGTA can attenuate the WFT-induced defense responses.
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Yang Yan-qi,Liang Yu-jian,Zhi Junrui,Li Ding-yin,Li Cao 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1
Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) is an important pest of crops that causes huge losses to grain production. Trehalose plays an important regulatory role in chitin synthesis. In this study, the mRNA expression of the trehalose synthase (TPS), soluble trehalase (TRE1), and membrane-bound trehalase (TRE2) genes in S. frugiperda were silenced by RNAi to reveal the mechanism by which trehalose metabolism regulates chitin synthesis. The results showed that the survival rate of S. frugiperda at different developmental stages decreased in varying degrees following RNAi, and abnormal phenotypes were detected. TRE1 activity was extremely significantly inhibited after dsTRE1 and dsTRE2 injection, and TRE2 activity was extremely signifi cantly inhibited after dsTPS and dsTRE2 injection. After silencing different trehalose metabolism-related regu latory genes, the trehalose content increased, whereas the glucose content decreased. Chitin synthase A (CHSA) gene was upregulated at 24 h after dsTPS and dsTRE1 injection. CHSA expression was significantly inhibited at 48 h after dsTRE1 injection. Chitin synthase B (CHSB) gene expression was significantly higher at 24 h after dsTRE1 injection. Furthermore, CHSB expression was significantly inhibited at 48 h after dsTPS, dsTRE1, or dsTRE2 injection. RNAi of TRE1 or TRE2 led to a significant decrease in chitin content. The results suggested that inhibiting the mRNA expression of genes related to trehalose metabolism in S. frugiperda could disrupt normal trehalose metabolism, thereby regulating the mRNA expression of downstream chitin synthase genes and further affecting chitin synthesis.
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