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Effect of carbon nanotube addition on friction coefficient of nanotubes/hydroxyapatite composites
Qian Zhao,Yalong Shen,Meiru Ji,Lei Zhang,Tingshun Jiang,Cangsheng Li 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.2
Carbon nanotubes/hydroxyapatite (CNTs/HA) composites with different CNTs contents were synthe-sized by in situ method and were characterized by XRD, TEM and Raman spectroscopy. Frictioncoefficients of the composites were tested using UMT-2 friction tester. Effect of various factors includingCNTs content, testing time and applied load on friction coefficient was carried out. Results show that theCNTs/HA composites exhibited lower friction coefficient than pure HA and their friction coefficientsdecreased with increase of CNTs content from 0 to 20 wt.%. Addition of CNTs in composite is beneficial toincrease wear resistance of the CNTs/HA composite and to decrease its friction coefficient.
Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy
Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.