miR-30c regulates proliferation, apoptosis and differentiation via the Shh signaling pathway in P19 cells

Xuehua Liu,Mengmeng Li,Yuzhu Peng,Xiaoshan Hu,Jing Xu,Shasha Zhu,Zhangbin Yu,Shuping Han 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that suppress protein expression by binding to the 3′ untranslated regions of their target genes. Many studies have shown that miRNAs have important roles in congenital heart diseases (CHDs) by regulating gene expression and signaling pathways. We previously found that miR-30c was highly expressed in the heart tissues of aborted embryos with ventricular septal defects. Therefore, this study aimed to explore the effects of miR-30c in CHDs. miR-30c was overexpressed or knocked down in P19 cells, a myocardial cell model that is widely used to study cardiogenesis. We found that miR-30c overexpression not only increased cell proliferation by promoting cell entry into S phase but also suppressed cell apoptosis. In addition, we found that miR-30c inhibited dimethyl sulfoxide-induced differentiation of P19 cells. miR-30c knockdown, in contrast, inhibited cell proliferation and increased apoptosis and differentiation. The Sonic hedgehog (Shh) signaling pathway is essential for normal embryonic development. Western blotting and luciferase assays revealed that Gli2, a transcriptional factor that has essential roles in the Shh signaling pathway, was a potential target gene of miR-30c. Ptch1, another important player in the Shh signaling pathway and a transcriptional target of Gli2, was downregulated by miR-30c overexpression and upregulated by miR-30c knockdown. Collectively, our study revealed that miR-30c suppressed P19 cell differentiation by inhibiting the Shh signaling pathway and altered the balance between cell proliferation and apoptosis, which may result in embryonic cardiac malfunctions.

Design and evaluation of a distributed TDR moisture sensor

Zhang, Bin,Yu, Xinbao,Yu, Xiong Techno-Press 2010 Smart Structures and Systems, An International Jou Vol.6 No.9

This paper describes the development and evaluation of an innovative TDR distributed moisture sensor. This sensor features advantages of being responsive to the spatial variations of the soil moisture content. The geometry design of the sensor makes it rugged for field installation. Good linear calibration is obtained between the sensor measured dielectric constant and soil physical properties. Simulations by the finite element method (FEM) are conducted to assist the design of this sensor and to determine the effective sampling range. Compared with conventional types of moisture sensor, which only makes point measurement, this sensor possesses distributed moisture sensing capability. This new sensor is not only easy to install, but also measures moisture distribution with much lower cost. This new sensor holds promise to significantly improve the current field instruments. It will be a useful tool to help study the influence of a variety of moisture-related phenomena on infrastructure performance.

Risk Factors for Cervical Cancer in Rural Areas of Wuhan China: a Matched Case-control Study

Zhang, Bin,Zhou, Ai-Fen,Zhu, Chang-Cai,Zhang, Ling,Xiang, Bing,Chen, Zhong,Hu, Rong-Hua,Zhang, Ya-Qi,Qiu, Lin,Zhang, Yi-Ming,Xiong, Chao-Du,Du, Yu-Kai,Shi, Yu-Qin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Cervical cancer is a serious public health problem in developing countries. We investigated possible risk factors for cervical cancer in rural areas of Wuhan China using a matched case-control study with 33 women diagnosed with cervical cancer and 132 healthy women selected from the same area as matched controls. A questionnaire, which included questions about general demography conditions, environmental and genetic factors, the first sexual intercourse, first marriage age, age at first pregnancy, pregnancy first child's age, female personal health history, social psychological factors, dietary habits, smoking and alcohol status and other living habits was presented to all participants. At the same time, HPV infection of every participant was examined in laboratory testing. Results showed HPV infection (P<0.000, OR=23.4) and pregnancy first child's age (P<0.000, OR=13.1) to be risk factors for cervical cancer. Menopause (P=0.003, OR=0.073) was a protective factor against cervical cancer. However, there was no indication of associations of environmental (drinking water, insecticide, disinfectant) genetic (cancer family history), or life-style factors (smoking status, alcohol status, physical training, sleep quality), including dietary habits (intake of fruit and vegetable, meat, fried food, bean products and pickled food) or social psychological factors with cervical cancer. The results suggest that the risk of cervical cancer in Chinese rural women may be associated with HPV infection, menopause and the pregnancy first child's age.

An integrated method of flammable cloud size prediction for offshore platforms

Zhang Bin,Zhang Jinnan,Yu Jiahang,Wang Boqiao,Li Zhuoran,Xia Yuanchen,Chen Li 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

Response Surface Method (RSM) has been widely used for flammable cloud size prediction as it can reduce computational intensity for further Explosion Risk Analysis (ERA) especially during the early design phase of offshore platforms. However, RSM encounters the overfitting problem under very limited simulations. In order to overcome the disadvantage of RSM, Bayesian Regularization Artificial Neural (BRANN)-based model has been recently developed and its robustness and efficiency have been widely verified. However, for ERA during the early design phase, there seems to be room to further reduce the computational intensity while ensuring the model's acceptable accuracy. This study aims to develop an integrated method, namely the combination of Center Composite Design (CCD) method with Bayesian Regularization Artificial Neural Network (BRANN), for flammable cloud size prediction. A case study with constant and transient leakages is conducted to illustrate the feasibility and advantage of this hybrid method. Additionally, the performance of CCD-BRANN is compared with that of RSM. It is concluded that the newly developed hybrid method is more robust and computational efficient for ERAs during early design phase.

ON A CLASS OF TERNARY CYCLOTOMIC POLYNOMIALS

ZHANG, BIN,ZHOU, YU Korean Mathematical Society 2015 대한수학회보 Vol.52 No.6

A cyclotomic polynomial ${\Phi}_n(x)$ is said to be ternary if n = pqr for three distinct odd primes p < q < r. Let A(n) be the largest absolute value of the coefficients of ${\Phi}_n(x)$. If A(n) = 1 we say that ${\Phi}_n(x)$ is flat. In this paper, we classify all flat ternary cyclotomic polynomials ${\Phi}_{pqr}(x)$ in the case $q{\equiv}{\pm}1$ (mod p) and $4r{\equiv}{\pm}1$ (mod pq).

Lack of Association of Common Polymorphisms in MUC1 Gene with H. pylori Infection and Non-cardia Gastric Cancer Risk in a Chinese Population

Zhang, Bin,Hao, Guang-Yu,Gao, Fang,Zhang, Jian-Zu,Zhou, Cheng-Jiang,Zhou, Li-She,Wang, Ying,Jia, Yan-Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Several lines of evidence support the notion that MUC1 is often aberrantly expressed in gastric cancer, and it is a ligand for Helicobacter pylori. Genetic variation in MUC1 gene may confer susceptibility to H. pylori infection and gastric cancer. We assessed the association of common polymorphisms in MUC1 gene with H. pylori infection and non-cardia gastric cancer using an LD-based tag SNP approach in north-western Chinese Han population. A total of four SNPs were successfully genotyped among 288 patients with non-cardia gastric cancer and 281 age- and sex-matched controls. None of the tested SNPs was associated with H. pylori infection. SNP rs9426886 was associated with a decreased risk of non-cardia gastric cancer, but lost significance after adjustment for multiple testing. Overall, our data indicated that common genetic variations in MUC1 gene might not make a major contribution to the risk of H. pylori infection and non-cardia gastric cancer in our studied population.

Experimental study on the combined effects of obstacles and local water mist on gas explosion for maritime safety

Zhang Bin,Li Zhuoran,Xia Yuanchen,Shi Jihao,Zhang Jinnan,Wang Boqiao,Yu Jiahang,Qu Yanxu,Chen Li,Lin Yejin,Wu Wanqing 대한조선학회 2022 International Journal of Naval Architecture and Oc Vol.14 No.1

A variety of experimental configurations including different water mist obstacles, are used to investigate the combined effects of obstacles and water mist upon the gas explosion. The results demonstrate the 8 mm water mist can significantly inhibit the deflagration, while both 45 mm and 80 mm water mists exhibit the opposite effect under all the locally distributed positions of water spray nozzle without obstacles inside the vessel. When considering the obstacles, the 45 mm water mist starts to mitigate the deflagration and its mitigation effect is more significant than that induced by the 8 mm water mist. What's more, the 80 mm water mist can slow down the flame propagation speed while it would still lead to the gas explosion. Additionally, there remains unchanged about the effect of locally spraying 8 mm water mist upon deflagration at the initial stage of flame development as varying the position of obstacles, while the effects of mitigating deflagration by 45 mm and 80 mm water mist are decreased. The results will make contributions to design the arrangement of equipment and water mist configuration on the offshore platform or NG-fueled ship's engine room so as to mitigate the gas explosion accident.

PWR core calculation based on pin-cell homogenization in three-dimensional pin-by-pin geometry

Zhang Bin,Li Yunzhao,Wu Hongchun,Zhao Wenbo,Fang Chao,Gong Zhaohu,Li Qing,Chai Xiaoming,Yu Junchong 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.6

For the pressurized water reactor two-step calculation, the traditional assembly homogenization and two-group neutron diffusion calculation have been widely used. When it comes to the core pin-by-pin simulation, many models and techniques are different and unsettled. In this paper, the homogenization methods based on the pin discontinuity factors and super homogenization factors are used to get the pin-cell homogenized parameters. The heterogeneous leakage model is applied to modify the infinite flux spectrum of the single assembly with reflective boundary condition and to determine the diffusion coefficients for the SP3 solver which is used in the core simulation. To reduce the environment effect of the single-assembly reflective boundary condition, the online method for the SPH factors updating is applied in this paper, and the functionalization of SPH factors based on the least-squares method will be pre-made alone with the table of the group constants. The fitting function will be used to update the thermal-group SPH factors with a whole-core pin-by-pin homogeneous solution online. The three-dimensional Watts Bar Nuclear Unit 1 (WBN1) problem was utilized to test the performance of pin-by-pin calculation. And numerical results have demonstrated that PWR pin-by-pin core calculation has more accurate results compared with the traditional assembly-homogenization scheme.