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Synthesis, structure and tribological performance of tungsten disulphide nanocomposites
Si, Ping-Zhan,Choi, Chul-Jin,Lee, Jae-Wook,Geng, Dian-Yu,Zhang, Zhi-Dong Elsevier Sequoia 2007 Materials science & engineering Structural materia Vol.443 No.1-2
<P><B>Abstract</B></P><P>Tungsten disulphide nanocomposites were prepared in large scale by employing the direct reaction between tungsten nanoparticles and sulphur powders. The nanocomposites include a variety of shapes such as isolated WS<SUB>2</SUB> multi-layered sheets, WS<SUB>2</SUB>-coated WO<SUB>3</SUB> nanoparticles, and enclosed fullerene-like WS<SUB>2</SUB> structures. Pin-on-disc wear tests indicate that the addition of a small amount (5wt%) of these nanoparticles to paraffin decreases significantly the wear loss of the pin. Additives of these WS<SUB>2</SUB>/WO<SUB>3</SUB> nanoparticles possess much better tribological performance than micrometer-sized WS<SUB>2</SUB> powders additives do. The surface topography of wear scar of the pins after wear tests with different additives and different loads were studied by scanning electron microscopy. Small (severe) damage of the pin surface was observed for WS<SUB>2</SUB>/WO<SUB>3</SUB> nanoparticles (WS<SUB>2</SUB> micropowders) additives.</P>
Si, Ping-Zhan,Qian, Hui-Dong,Choi, Chul-Jin,Park, Jihoon,Ge, Hong-Liang North-Holland Pub. Co 2018 Journal of magnetism and magnetic materials Vol.451 No.-
<P><B>Abstract</B></P> <P>The temperature for phase transformation of ε → τ in Mn<SUB>54</SUB>Al<SUB>46</SUB>C<I> <SUB>x</SUB> </I> (hereafter denoted by MnAlC<I> <SUB>x</SUB> </I>, <I>x</I> = 0–5) alloys was systematically studied by using the traditional thermal analysis method and a more precise magnetic measurement method developed in this work. The effect of carbon content and cold-rolling on the magnetic properties of the MnAlC<I> <SUB>x</SUB> </I> alloys was studied. The onset temperature of massive growth of τ- from ε-phase increases with increasing carbon content in MnAlC<I> <SUB>x</SUB> </I>. The carbon content has little effect on the onset temperature of displacive growth of ε → τ but has substantial effect on the displacive growth rate at certain temperature. Pure τ-MnAlC<SUB> <I>x</I> </SUB> with varied <I>x</I> was obtained by annealing the ε-phase at optimum temperatures determined by our method. The Curie temperature of the MnAlC<SUB> <I>x</I> </SUB> decreases significantly first and then increases slightly with increasing carbon content. The coercivity and the remanent magnetization of the as-prepared τ-MnAlC<SUB> <I>x</I> </SUB> increase with increasing <I>x</I> first and become stable at <I>x </I>> 3 and <I>x</I> > 4, respectively. A cold-rolling method was introduced in this work to enhance the magnetic properties of τ-MnAlC<SUB> <I>x</I> </SUB>. The coercivity and remanent magnetization of the τ-MnAl after cold-rolling were enhanced up to 4.3 times and 3.3 times, respectively. The coercivity of the cold-rolled τ-MnAlC<SUB> <I>x</I> </SUB> decreases with increasing <I>x</I> and becomes stable when <I>x</I> > 2. The remanent magnetization and saturation magnetization of the cold-rolled τ-MnAlC<SUB> <I>x</I> </SUB> vary little with <I>x</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A method to measure the phase transformation temperature of MnAl-C is reported. </LI> <LI> A cold-rolling method is employed to enhance the magnetic properties of MnAl-C. </LI> <LI> The phase transformation temperature increases with increasing carbon content. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
LncRNA SNHG12/miR-494-3p/CBX3 axis in diffuse large B-cell lymphoma
Si Cheng,Zhang Wanyong,Han Qi,Zhu Bisheng,Zhan Chengzhi 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.1
Background Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy in adults. Current studies have implicated that long non-coding RNAs (lncRNAs) regulate the development of DLBCL; nevertheless, the control method is dim. Here, we reconnoitered the characters of lncRNA small nucleolar RNA host gene 12 (lncRNA SNHG12) in DLBCL. Objective The pathological morphology of tumor tissues and normal tissues was perceived by hematoxylin–eosin (HE) staining. The levels of SNHG12, microRNA-494-3p (miR-494-3p), and chromobox 3 (CBX3) were detected by quantitative real-time PCR and western blot. Whereafter, MTT assay and EdU assay were enforced to validate cell proliferation. Flow cytometry assay was implemented to assess the cell cycle and cell apoptosis, respectively. Additionally, the interface between miR-494-3p with SNHG12 or CBX3 was identified by dual-luciferase reporter assay. Results SNHG12 and CBX3 were enhanced, but miR-494-3p was diminished in DLBCL. Knockdown of SNHG12 repressed cell proliferation and cell cycle, although heightened cell apoptosis in DLBCL cells. SNHG12 sponged miR-494-3p to adjust the CBX3. Additionally, miR-494-3p restrained DLBCL cells development by targeting CBX3. Conclusion SNHG12 contributed to DLBCL development by regulating miR-494-3p/CBX3. The outcomes demonstrated that SNHG12 provided a potential train of thought for DLBCL theory of targeted therapy.
Cloning of Phospholipase D from Grape Berry and Its Expression under Heat Acclimation
Wan, Si-Bao,Wang, Wei,Wen, Peng-Fei,Chen, Jian-Ye,Kong, Wei-Fu,Pan, Qiu-Hong,Zhan, Ji-Cheng,Tian, Li,Liu, Hong-Tao,Huang, Wei-Dong Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.4
To investigate whether phospholipase D (PLD, EC 3.1.4.4) plays a role in adaptive response of post-harvest fruit to environment, a PLD gene was firstly cloned from grape berry (Vitis Vinifera L. cv. Chardonnay) using RT-PCR and 3'- and 5'-RACE. The deduced amino acid sequence (809 residues) showed 84.7% identity with that of PLD from Ricinus communis. The secondary structures of this protein showed the characteristic C2 domain and two active sites of a phospholipid-metabolizing enzyme. The PLD activity and its expression in response to heat acclimation were then assayed. The results indicated PLD was significantly activated at enzyme activity, as well as accumulation of PLD mRNA and synthesis of new PLD protein during the early of heat acclimation, primary suggesting that the grape berry PLD may be involved in the heat response in post-harvest grape berry. This work offers an important basis for further investigating the mechanism of post-harvest fruit adaptation to environmental stresses.