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Chao Liang,Shipeng Han,Hui Han,Feng Zhao,Yunzhuan He 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4
The selection of a stable reference gene is vital to gene expression studies and to improving the accuracy of RTqPCR data. With the deep research on the artificial feed of the Harmonia axyridis, there is an increasing need to evaluate the effects of feed on insects at the molecular level. To identify a reference gene to assess the expression of related genes in Harmonia axyridis (Pallas), ensure the reliability of target gene expression analysis using realtime PCR. Especially for H. axyridis were fed Rhopalosiphum padi (L.) or an artificial diet. In this study, the expression profiles of nine candidate reference genes, including 28SrRNA, 18SrRNA, RPS23, EF1, Actin, ATPase, GAPDH, UBI, RPL13 from different H. axyridis tissues (head, thorax, wing, leg, ovary, and fat body) were investigated. The stability of the nine candidate genes was assessed using geNorm, NormFinder, BestKeeper and RefFinder software, and a comprehensive analysis showed that EF1 is a suitable reference gene for eating different diets of different organizations from H. axyridis. 28SrRNA, 18SrRNA, and RPS23 can also be used as reference genes, but Actin, ATPase, RPL13 are not suitable as an internal reference gene
Zhou Yayuan,Song Peng,Luo Dan,Feng Zhaoyang,Wang Da,He Yunzhuan 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
Athetis lepigone is an important pest on corn, but little is known about the nutritional requirements of adult A. lepigone and how nutrients affect their reproduction and longevity. Here, we explored the effects of different nutrients on the reproduction, longevity, and population growth of A. lepigone. Carbohydrates were beneficial for adult reproduction and longevity, with feeding 5% sucrose having the best effect by resulting in an average of 424.83 eggs per female. When females consumed carbohydrates, the net reproductive rate (R 0 ) and the intrinsic rate of increase (r m ) were higher. However, the fecundity of adults fed only amino acids and high concentration fat emulsion decreased significantly, with an average of 60 to 160 eggs per female. The main food source of Lepidoptera insects is the nectar from flowering plants in the field. Due to compound planting patterns, flowering plants are widely distributed around farmland. The nectar secreted by them usually contains rich carbohydrates, which can be provided for adults. Therefore, we must also consider the potential impact of flowering plants on A. lepigone, in order to improve pest control efficiency.