Combined treatment with β-carotene and curcumin enhance reproductive potential on heat stress-induced testicular oxidative stress in mice

Yun Seok Choi,Chunmei Lin,Sl Bi Chu,Jung-Min Yon,Seul Gi Park,Ju Yeol Han,Hye Jong Oh,Beom Jun Lee,Young Won Yun,Sang-Yoon Nam 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

The Conjugated Double Bond of Coniferyl Aldehyde Is Essential for Heat Shock Factor 1 Mediated Cytotoprotection

Choi, Seul-Ki,Mun, Gil-Im,Choi, Eun,Kim, Seo-Young,Kwon, Youngjoo,Na, Younghwa,Lee, Yun-Sil American Society of Pharmacognosy 2017 Journal of natural products Vol.80 No.8

<P>Coniferyl aldehyde (1) is previously reported as a potent inducer of heat shock factor 1 (HSF1). Here, we further examined the active pharmacophore of 1 for activation of HSF1 using the derivatives coniferyl alcohol (2), 4-hydroxy-3-methoxyphenylpropanal (3), and 4-hydroxy-3-methoxyphenylpropanol (4). Both 1 and 2 resulted in increased survival days after a lethal radiation (IR) dose. The decrease in bone marrow (BM) cellularity and Ki67-positive BM cells by IR was also significantly restored by 1 or 2 in mice. These results suggested that the vinyl moiety of 1 and 2 is necessary for inducing HSF1, which may be useful for developing small molecules for cytoprotection of normal cells against damage by cytotoxic drugs and radiation.</P>

Aqueous extract of <i>Codium fragile</i> suppressed inflammatory responses in lipopolysaccharide-stimulated RAW264.7 cells and carrageenan-induced rats

Lee, Seul Ah,Moon, Sung-Min,Choi, Yun Hee,Han, Seul Hee,Park, Bo-Ram,Choi, Mi Suk,Kim, Jae-Sung,Kim, Yong Hwan,Kim, Do Kyung,Kim, Chun Sung Elsevier 2017 BIOMEDICINE AND PHARMACOTHERAPY Vol.93 No.-

<P><B>Abstract</B></P> <P> <I>Codium fragile</I> (Suringar) Hariot has been used in Oriental medicine for the treatment of enterobiasis, dropsy, and dysuria and has been shown to have various biological effects. In this study, we evaluated the anti-inflammatory effects of aqueous extract of <I>C. fragile</I> (AECF) using <I>in vitro</I> and <I>in vivo</I> models. Nitric oxide (NO), prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), inflammatory-related mRNAs, and proteins were determined using the Griess assay, enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR), and western blotting, respectively. Our results indicate that pretreatment of cells with AECF (50, 100 and 200μg/mL) significantly inhibited LPS-induced secretion of NO and PGE<SUB>2</SUB> in RAW264.7 cells without cytotoxicity. We also found that AECF (100 and 200μg/mL) inhibited LPS-induced inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 expression in a dose-dependent manner. Additionally, pretreatment of cells with AECF (100 and 200μg/mL) inhibited LPS-induced production of inflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6. It also prevented the nuclear translocation of nuclear factor (NF)-κB by suppressing the phosphorylation and degradation of inhibitor of NF-κB (IκB)-α. Furthermore, AECF (100 and 200μg/mL) inhibited the phosphorylation of the mitogen-activated protein kinases (MAPKs) extracellular signal-regulated kinase (ERK) 1/2, c-Jun N-terminal kinase (JNK), and p38. In addition, orally administered 50, 100, and 200mg/kg body weight of AECF dose-dependently suppressed carrageenan-induced rat paw edema thickness by 6%, 31%, and 50% respectively, after 4h. Furthermore, the anti-inflammatory effect was comparable to that observed in animals treated with the standard drug diclofenac sodium (56%) <I>in vivo</I>. Collectively, our results suggest that AECF exerts potential anti-inflammatory effects by suppressing NF-κB activation and MAPKs pathways <I>in vitro</I>, as well as inhibiting carrageenan-induced rat paw edema thickness <I>in vivo</I>. These findings indicate that AECF could be further developed as an anti-inflammatory drug.</P>

인터랙티브 무비의 속성변인이 태도 및 구전의도에 미치는 영향에 관한 연구

최윤슬(Choi, Yun-Seul),김운한(Kim, Woon-Han) 한국광고홍보학회 2014 광고연구 Vol.0 No.103

본 연구는 디지털 환경 내 가상 체험을 제공하는 인터랙티브 무비의 커뮤니케이션 효과를 밝히는 데 목적이 있다. 이를 위해 인터랙티브 무비의 구성요인으로 지각된 개인화, 지각된 실재감, 공감성, 오락성, 상호작용성, 정보성을 추출하고 콘텐츠 태도와 구전의도에 미치는 영향 관계를 조사하였다. 분석 결과, 인터랙티브 무비에 대해 소비자가 경험하는 지각된 개인화, 공감성, 오락성, 정보성이 콘텐츠 태도에 유의미한 영향을 미치며, 지각된 개인화, 지각된 실재감, 상호작용성, 공감성, 오락성은 구전의도에 유의미한 영향을 미치는 것으로 나타났다. 또한 인터랙티브 무비의 항목에서 정보성을 제외한 모든 속성변인이 콘텐츠 태도를 매개하여 구전의도에 유의미한 인과관계를 갖는 것으로 나타났다. 분석 결과를 통해 지각된 개인화와 지각된 실재감, 상호작용성과 공감성을 강화하는 것이 가상환경을 이용한 인터랙티브 커뮤니케이션 채널로서 인터랙티브 무비의 커뮤니케이션 효과를 제고하기 위해 필요함을 알 수 있었다. 이와 함께 새로운 미디어 플랫폼의 속성 및 소비자 개인의 특성을 고려한 맞춤형 전략을 통해 콘텐츠 태도와 구전의도가 증가할 수 있다는 시사점을 발견하였다. This study aimed to examine the influence of interactive movies on attitude toward contents(Ac) and intention of word-of-mouth(Iwom). For this purpose, the study extracted the components of interactive motives, such as the perceived personalization, perceived presence, sympathy, perceived interactivity, entertainment, informativeness. With these variables, Ac and Iwom were examined in virtual experience during the exposure of interactive movies. Results showed that the perceived personalization, sympathy, entertainment, and informativeness of interactive movies in virtual environment had a significant impact on Ac. The perceived personalization, perceived presence, perceived interactivity, sympathy, and entertainment were found to have a significant influence on Iwom. In addition, all components of interactive movies were found to have a positive effect on Iwom through Ac. Aforesaid results suggest that more impactful advertising effects can be achieved in a new digital marketing environment if the perceived personalization, perceived presence, perceived interactivity, and sympathy will be strengthened through impactful virtual experience in an interactive communication channel such as interactive movies.

Growth Pattern of the Neo-Aorta after Arterial Switch Operation during Childhood

Seul Gi Cha,Jae Suk Baek,Jeong Jin Yu,Young-Hwue Kim,Bo Sang Kwon,Eun Seok Choi,Chun Soo Park,Tae-Jin Yun 대한심장학회 2021 Korean Circulation Journal Vol.51 No.1

Background and Objectives: Neo-aortic root dilatation (ARD) and annular dilatation (AAD) tend to develop after arterial switch operation (ASO). However, the trend of neo-aortic growth has not been well established. This paper aims to identify this trend, its associated factors, and predictors of neo-aortic dilatation after ASO. Methods: We analyzed the growth trend of the neo-aortic root, annulus, and sinotubular junction (STJ) z-scores using random coefficients model and the risk factors affecting neo-aortic dilatation in 163 patients who underwent ASO from 2006 to 2015. Results: Among 163 patients, 41 had a ventricular septal defect, and 11 had Taussig-Bing (TB) anomaly. The median follow-up duration was 6.61 years. The increased in the neo-aortic root z-score was different between the trapdoor and non-trapdoor coronary artery transfer techniques (0.149/year, p<0.001 vs. 0.311/year, p<0.001). Moreover, the neo-aortic annulus and STJ z-score significantly increased over time after ASO (0.067/year, p<0.001; 0.309/year, p<0.001). Pulmonary artery banding (PAB) was rather a negative affecting factor. The probabilities of freedom from ARD, AAD, and neo-aortic STJ dilatation at 10 years after ASO were 33.4%, 53.9%, and 65.4%. Neo- aortic regurgitation within 1 year was the predictor of ARD, AAD, and neo-aortic STJ dilatation. TB anomaly, PAB, and native pulmonary sinus z-score were other predictors for ARD. Conclusion: The growth of neo-aortic root, annulus, and STJ after ASO was greater than somatic growth during childhood. The coronary artery transfer technique affected the growth pattern of the neo-aortic root.

Eu²⁺-Activated Phase-Pure Oxonitridosilicate Phosphor in a Ba–Si–O–N System via Facile Silicate-Assisted Routes Designed by First-Principles Thermodynamic Simulation

Yun, Young Jun,Kim, Jin Kyu,Ju, Ji Young,Choi, Seul Ki,Park, Woon Ik,Jung, Ha-kyun,Kim, Yongseon,Choi, Sungho American Chemical Society 2016 Inorganic Chemistry Vol.55 No.17

<P>Eu2+-activated single phase Ba2+-oxonitridosilicate phosphors were prepared under a mild synthetic condition via silicate precursors, and their luminescent properties were investigated. Both the preferred oxonitridosilicate formation as for the available host compounds and thermodynamic stability within the Ba-Si-O-N system were elucidated in detail by the theoretical simulation based on the first-principles density functional theory. Those results can visualize the optimum synthetic conditions for Eu2+-activated highly luminescent Ba2+-oxonitridosilicates, especially Ba3Si6O12N2, as promising conversion phosphors for white LEDs, including Ba3Si6O9N4 and BaSi2O2N2 phases. To prove the simulated design rule, we synthesized the Ba3Si6O12N2:Eu2+ phosphor using various silicate precursors, Ba2Si4O10, Ba2Si3O8, and BaSiO3, in a carbothermal reduction ambient and finally succeeded in obtaining a phase of pure highly luminescent oxonitridosilicate phosphor without using any solid-state nitride addition and/or high pressure synthetic procedures. Our study provides useful guidelines for robust synthetic procedures for developing thermally stable rare-earth-ion activated oxonitridosilicate phosphors and an established simulation method that can be effectively applied to other multigas systems.</P>

Development and Evaluation of Deep Learning-Based Automatic Segmentation Model for Skull Zero TE MRI in Children

Yun Seok Seo,Young Hun Choi,Joon Sung Lee,Seul Bi Lee,Yeon Jin Cho,Seunghyun Lee,Su-Mi Shin,Jung-Eun Cheon 대한자기공명의과학회 2023 Investigative Magnetic Resonance Imaging Vol.27 No.1

Purpose: To develop and evaluate a deep learning technique to automatically segment bone structures in zero echo time (ZTE) for skull magnetic resonance imaging (MRI) in children. Materials and Methods: From January to December 2021, 38 bone ZTE MRIs from infants and children (age range, 1–31 months) were collected for model development. Mask images were generated by manually segmenting the craniofacial bone using a commercial segmentation program. Among them, 35 ZTE series were used to train the three-dimensional (3D)-nnUnet deep learning model and the remaining three series were used for model validation. A temporally different dataset of 19 ZTE bone MRIs obtained in May 2022 from infants and children (age range, 3–168 months) was used to determine the model’s performance. Dice similarity coefficient was calculated for each test case. From 3D volume rendering images, segmentation accuracy, overall image quality, and visibility of cranial sutures were subjectively evaluated on a 5-point scale and compared with ground truth data from manual segmentation. Reasons for segmentation failure were analyzed using axially segmented ZTE images. Results: For the test set, the mean Dice similarity coefficient was 0.985 ± 0.019. The segmentation accuracy was lower than the ground truth without showing a statistically significant difference between the two (3.39 ± 1.11 vs. 3.73 ± 0.77, p = 0.055). The overall image quality and suture visibility showed no significant difference (3.34 ± 0.75 vs. 3.42 ± 0.69, p = 0.317; 3.55 ± 0.97 vs. 3.60 ± 0.95, p = 0.157). Common reasons for low segmentation accuracy were well-pneumatized sinuses, metal artifacts, skin at the vertex level, and bones too thin. Conclusion: The deep learning-based automatic segmentation technique of bone ZTE MRIs showed comparable segmentation performance to manual segmentation. Using the deep learning-based segmentation results, acceptable 3D-volume rendering images of craniofacial bones were generated.

Visualization of the Biological Behavior of Tumor-Associated Macrophages in Living Mice with Colon Cancer Using Multimodal Optical Reporter Gene Imaging

Choi, Yun Ju,Oh, Seul-Gi,Singh, Thoudam Debraj,Ha, Jeoung-Hee,Kim, Dong Wook,Lee, Sang Woo,Jeong, Shin Young,Ahn, Byeong-Cheol,Lee, Jaetae,Jeon, Young Hyun Neoplasia Press 2016 Neoplasia Vol.18 No.3

<P>We sought to visualize the migration of tumor-associated macrophages (TAMs) to tumor lesions and to evaluate the effects of anti-inflammatory drugs on TAM-modulated tumor progression in mice with colon cancer using a multimodal optical reporter gene system. Murine macrophage Raw264.7 cells expressing an enhanced firefly luciferase (Raw/effluc) and murine colon cancer CT26 cells coexpressing Rluc and mCherry (CT26/Rluc-mCherry, CT26/RM) were established. CT26/RM tumor-bearing mice received Raw/effluc via their tail veins, and combination of bioluminescence imaging (BLI) and fluorescence imaging (FLI) was conducted for <I>in vivo</I> imaging of TAMs migration and tumor progression. Dexamethasone (DEX), a potent anti-inflammatory drug, was administered intraperitoneally to tumor-bearing mice following the intravenous transfer of Raw/effluc cells. The migration of TAMs and tumor growth was monitored by serial FLI and BLI. The migration of Raw/effluc cells to tumor lesions was observed at day 1, and BLI signals were still distinct at tumor lesions on day 4. Localization of BLI signals from migrated Raw/effluc cells corresponded to that of FLI signals from CT26/RM tumors. <I>In vivo</I> FLI of tumors demonstrated enhanced tumor growth associated with macrophage migration to tumor lesions. Treatment with DEX inhibited the influx of Raw/effluc cells to tumor lesions and abolished the enhanced tumor growth associated with macrophage migration. These findings suggest that molecular imaging approach for TAM tracking is a valuable tool for evaluating the role of TAMs in the tumor microenvironment as well as for the development of new drugs to control TAM involvement in the modulation of tumor progression.</P>

Potential Inhibition of PDK1/Akt Signaling by Phenothiazines Suppresses Cancer Cell Proliferation and Survival

Choi, Jang Hyun,Yang, Yong Ryoul,Lee, Seul Ki,Kim, Sun-Hee,Kim, Yun-Hee,Cha, Joo-Young,Oh, Se-Woong,Ha, Jong-Ryul,Ryu, Sung Ho,Suh, Pann-Ghill Wiley (New York Academy of Sciences) 2008 Annals of the New York Academy of Sciences Vol.1138 No.1

<P>3'-Phosphoinositide-dependent kinase-1 (PDK1) has been identified for its ability to phosphorylate and activate Akt. Accumulated studies have shown that the activation of the PDK1/Akt pathway plays a pivotal role in cell survival, proliferation, and tumorigenesis. Therefore, the PDK1/Akt pathway is believed to be a critical target for cancer intervention. In this paper, we report the discovery of a new function of phenothiazines, widely known as antipsychotics, inhibiting PDK1/Akt pathway. Upon epidermal growth factor (EGF) stimulation, phenothiazines specifically suppressed the kinase activity of PDK1 and the phosphorylation level of Akt. The inhibition of PDK1/Akt kinase resulted in suppression of EGF-induced cell growth and induction of apoptosis in human ovary cancer cells. In particular, phenothiazines were highly selective for downstream targets of PDK1/Akt and did not inhibit the activation of phosphatidylinositol 3-kinase (PI3K), EGFR, or extracellular signal-regulated kinase 1/2 (ERK1/2). In particular, phenothiazines effectively suppressed tumor growth in nude mice of human cancer cells. Taken together, these findings provide strong evidence for novel function of phenothiazines, pharmacologically targeting PDK1/Akt for anticancer drug discovery.</P>

Toxic effects of microplastic (polyethylene) exposure: Stress, immune responses and neurotoxicity in Korean Bullhead, Pseudobagrus fulvidraco

Yun-A Ryu,A-Hyun Jo,Ji-Ho Jeong,Seul Gi Na Ra Park,Serin Choi,Jung Woo Seo,Ji won Park,Jun-Hwan Kim (사)한국해양생명과학회 2023 (사)한국해양생명과학회 학술대회 Vol.2023 No.06