http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Yuhe Liu (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Promoter effects of adeno-associated viral vector for transgeneexpression in the cochlea in vivo
Yuhe Liu,Takashi Okada,Tatsuya Nomoto,Xiaomei Ke,Akihiro Kume,Keiya Ozawa,Shuifang Xiao 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.2
The aims of this study were to evaluate the expression of enhanced green fluor escent protein (EGFP) driven by 6 different promoters, including cytomegalovirus IE enhancer and chicken βactin promoter (CAG), cytomegalovirus promoter (CMV), neuron-specific enolase promoter (NSE), myosin 7A promoter (Myo), elongation factor 1α promoter (EF-1α), and Rous sarcoma virus promoter (RSV), and assess the dose in the cochlea. Serotype 1 adeno-associated virus (AAV1) vectors with various constructs were transduced into the cochl eae, and the level of EGFP expression was examined. We found the highest EGFP expression in the inner hair cells and other cochlear cells when CAG promoter was used. The CMV and NSE promoter drove the higher EGFP expression, but only a marginal activity was ob-served in EF-1α promoter driven constructs. RSV promoter failed to drive the EGFP expression. Myo promoter driven EGFP was exclusively expressed in the inner hair cells of the cochlea. When driven by CAG promoter, reporter gene expression was detected in inner hair cells at a dose as low as 3 107 genome copies, and continued to increase in a dose- dependent manner. Our data showed that individual expression in the cochlear cells. Our results might provide important information with regard to the role of promoters in regulating transgene expression and for the proper design of vectors for gene expression and gene therapy.
-
Facile Fabrication of Non-fluorinated Durable Superhydrophobic Cotton Fabric
Guolin Zheng,Yuhe Wu,Dan Zhang,Shuai Liu,Zhu Long,Rong Li 한국섬유공학회 2020 Fibers and polymers Vol.21 No.11
The superhydrophobic surface is easy to lose the superhydrophobicity in the practical applications, due to the factthat the micro- and nanoscale rough structure is easily damaged. Therefore, it is one of the challenges to endow solid surfacewith durable superhydrophobic property. In this work, the environment-friendly superhydrophobic cotton fabric was preparedby dip-coating methodology. Meanwhile, the cotton fabric was endowed with durable superhydrophobicity. Herein, theoctadecylamine was utilized to reduce the surface free energy of cotton fabric. However, octadecylamine was only adsorbedon the fabric surface. Therefore, the hydrophobic resin was employed to supply enough adhesion force. As a result, thelimitation that traditional methods frequently utilize additional nanoparticles to construct hierarchical rough surfaces wasbroken. The water contact angles of the coated cotton fabric at room temperature and 70 oC reached 154.4±0.6 o and120±1.1 o, respectively. Moreover, the coated cotton fabric still reserved superhydrophobic property after 30th cycle’sabrasion and exhibited excellent hydrophobicity after 500th cycle’s abrasion. In addition, the surface free energy of originalcotton fabric reduced by 54.7 % so that the coated cotton fabric had outstanding repellency to hydrochloric acid, sodiumhydroxide solution, coffee and sodium chloride solution, respectively.
-
Jin, Li,Piao, Zhe Hao,Liu, Chun Ping,Sun, Simei,Liu, Bin,Kim, Gwi Ran,Choi, Sin Young,Ryu, Yuhee,Kee, Hae Jin,Jeong, Myung Ho John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.3
<P><B>Abstract</B></P><P>Hypertension causes cardiac hypertrophy and leads to heart failure. Apoptotic cells are common in hypertensive hearts. Ca<SUP>2+</SUP>/calmodulin‐dependent protein kinase II (CaMKII) is associated with apoptosis. We recently demonstrated that gallic acid reduces nitric oxide synthase inhibition‐induced hypertension. Gallic acid is a trihydroxybenzoic acid and has been shown to have beneficial effects, such as anti‐cancer, anti‐calcification and anti‐oxidant activity. The purpose of this study was to determine whether gallic acid regulates cardiac hypertrophy and apoptosis in essential hypertension. Gallic acid significantly lowered systolic and diastolic blood pressure in spontaneously hypertensive rats (SHRs). Wheat germ agglutinin (WGA) and H&E staining revealed that gallic acid reduced cardiac enlargement in SHRs. Gallic acid treatment decreased cardiac hypertrophy marker genes, including atrial natriuretic peptide (<I>ANP</I>) and brain natriuretic peptide (<I>BNP</I>), in SHRs. The four isoforms, α, β, δ and γ, of <I>CaMKII</I> were increased in SHRs and were significantly reduced by gallic acid administration. Gallic acid reduced cleaved caspase‐3 protein as well as <I>bax</I>,<I> p53</I> and <I>p300 </I>mRNA levels in SHRs. <I>CaMKII</I> δ overexpression induced <I>bax</I> and <I>p53</I> expression, which was attenuated by gallic acid treatment in H9c2 cells. Gallic acid treatment reduced DNA fragmentation and the TUNEL positive cells induced by angiotensin II. Taken together, gallic acid could be a novel therapeutic for the treatment of hypertension through suppression of CaMKII δ‐induced apoptosis.</P>
-
<i>Dendropanax morbifera</i> Prevents Cardiomyocyte Hypertrophy by Inhibiting the Sp1/GATA4 Pathway
Sun, Simei,Li, Tianyi,Jin, Li,Piao, Zhe Hao,Liu, Bin,Ryu, Yuhee,Choi, Sin Young,Kim, Gwi Ran,Jeong, Ji Eun,Wi, An Jin,Lee, Song Ju,Kee, Hae Jin,Jeong, Myung Ho World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.5
<P>An extract of <I>Dendropanax morbifera</I> branch exerts antioxidant, anti-inflammatory, antithrombotic, and anticancer activities. The purpose of this study was to investigate the effect of the extract in isoproterenol-induced cardiac hypertrophy. Phalloidin staining showed that treatment with the extract dramatically prevents isoproterenol-induced H9c2 cell enlargement and the expression of cardiac hypertrophic marker genes, including atrial natriuretic peptide (ANP) and B-type brain natriuretic peptide (BNP). Further, pretreatment with the extract decreased isoproterenol-induced GATA4 and Sp1 expression in H9c2 cells. Overexpression of Sp1 induced the expression of GATA4. The forced expression of Sp1 or its downstream target GATA4, as well as the co-transfection of Sp1 and GATA4 increased the expression of ANP, which was decreased by treatment with the extract. To further elucidate the regulation of the Sp1/GATA4-mediated expression of ANP, knockdown experiments were performed. Transfection with small interfering RNAs (siRNAs) for Sp1 or GATA4 decreased ANP expression. The extract did not further inhibit the expression of ANP reduced by the transfection of GATA4 siRNA. Sp1 knockdown did not affect the expression of ANP that was induced by the overexpression of GATA4; however, GATA4 knockdown abolished the expression of ANP that had been induced by Sp1 overexpression. The extract treatment also attenuated the isoproterenol-induced activation of p38 MAPK, ERK1/2, and JNK1. Hesperidin, catechin, 2,5-dihydroxybenzoic acid, and salicylic acid are the main phenolic compounds present in the extract as observed by high performance liquid chromatography. Hesperidin and 2,5-dihydroxybenzoic acid attenuated isoproterenol-induced cardiac hypertrophy. These findings suggest that the <I>D. morbifera</I> branch extract prevents cardiac hypertrophy by downregulating the activation of Sp1/GATA4 and MAPK signaling pathways.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
