IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1^-/- Mice Mediated by miR-33

Tang, Chen-Yi,Man, Xiao-Fei,Guo, Yue,Tang, Hao-Neng,Tang, Jun,Zhou, Ci-La,Tan, Shu-Wen,Wang, Min,Zhou, Hou-De Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.2

Insulin signaling is coordinated by insulin receptor substrates (IRSs). Many insulin responses, especially for blood glucose metabolism, are mediated primarily through Irs-1 and Irs-2. Irs-1 knockout mice show growth retardation and insulin signaling defects, which can be compensated by other IRSs in vivo; however, the underlying mechanism is not clear. Here, we presented an Irs-1 truncated mutated mouse ($Irs-1^{-/-}$) with growth retardation and subcutaneous adipocyte atrophy. $Irs-1^{-/-}$ mice exhibited mild insulin resistance, as demonstrated by the insulin tolerance test. Phosphatidylinositol 3-kinase (PI3K) activity and phosphorylated Protein Kinase B (PKB/AKT) expression were elevated in liver, skeletal muscle, and subcutaneous adipocytes in Irs-1 deficiency. In addition, the expression of IRS-2 and its phosphorylated version were clearly elevated in liver and skeletal muscle. With miRNA microarray analysis, we found miR-33 was down-regulated in bone marrow stromal cells (BMSCs) of $Irs-1^{-/-}$ mice, while its target gene Irs-2 was up-regulated in vitro studies. In addition, miR-33 was down-regulated in the presence of Irs-1 and which was up-regulated in fasting status. What's more, miR-33 restored its expression in re-feeding status. Meanwhile, miR-33 levels decreased and Irs-2 levels increased in liver, skeletal muscle, and subcutaneous adipocytes of $Irs-1^{-/-}$ mice. In primary cultured liver cells transfected with an miR-33 inhibitor, the expression of IRS-2, PI3K, and phosphorylated-AKT (p-AKT) increased while the opposite results were observed in the presence of an miR-33 mimic. Therefore, decreased miR-33 levels can up-regulate IRS-2 expression, which appears to compensate for the defects of the insulin signaling pathway in Irs-1 deficient mice.

Roles of GST-π and polβ Genes in Chemoresistance of Esophageal Carcinoma Cells

Tang, Yue,Xuan, Xiao-Yan,Li, Min,Dong, Zi-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

The main aim of this study was to investigate the roles of GST-${\pi}$ and $pol{\beta}$ genes in the chemoresistance of esophageal carcinoma cells. Eukaryotic expression vectors containing each gene were constructed and transfected into EC9706 cells, and the biological effects of the two genes assessed based on a resistance index. We additionally investigated the in vitro and in vivo anti-resistance effects of GST-${\pi}$ and $pol{\beta}$ genes using recombinant lentiviruses carrying siRNAs against the two genes. Our results showed that upregulation of GST-${\pi}$ and $pol{\beta}$ genes suppresses chemosensitivity of esophageal carcinoma cells to cisplatin, while downregulation of these two genes with RNAi technology reverses this chemoresistance. Multi-site injection of recombinant lentivirus targeting the GST-${\pi}$ gene into transplanted cDDP tumors effectively reversed their chemoresistant phenotype. However, the same treatment against the $pol{\beta}$ gene did not lead to significant efficacy against chemoresistance.

Optimization of impulse water turbine based on GA-BP neural network arithmetic

Lingdi Tang,Shouqi Yuan,Yue Tang,Zhipeng Qiu 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

To develop an optimum design method for impulse water turbines with low specific speed, a representative impulse water turbine with low specific speed used in agricultural irrigation machinery was optimized with a combination of an orthogonal experimental design, a genetic algorithm, and a BP neural network in this study. Numerical calculation was applied to analyze interflow characteristics for optimized and original water turbines. Results showed that the internal flow characteristics of the optimized water turbine presented remarkable improvement compared with the original water turbine. Pressure distribution increased, the vortex strip in the draft tube was reduced remarkably, and impeller torque increased by 26 %. In addition, the optimized impeller was manufactured by 3D printing, and performance comparison was conducted between experiments of the optimized and original water turbines. The efficiency of the optimized water turbine reached 42.5 %, which exceeded the original water turbine’s of 8.5 %. With increasing rotating speed, maximum efficiency running point moved to a high flow rate, and highly efficient areas expanded. Internal characteristic analysis and a full-scale experiment for both water turbines showed that the performance of the optimized water turbine exhibited substantial improvement. The analysis and experiment also verified the theoretical correctness and feasibility of the proposed optimum design method.

IRS-2 Partially Compensates for the Insulin Signal Defects in IRS-1−/−Mice Mediated by miR-33

Chen-Yi Tang,Xiao-Fei Man,Yue Guo,Hao-Neng Tang,Jun Tang,Ci-La Zhou,Shu-Wen Tan,Min Wang,Hou-De Zhou 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.2

Insulin signaling is coordinated by insulin receptor substrates (IRSs). Many insulin responses, especially for blood glucose metabolism, are mediated primarily through Irs-1 and Irs-2. Irs-1 knockout mice show growth retardation and insulin signaling defects, which can be compensated by other IRSs in vivo; however, the underlying mechanism is not clear. Here, we presented an Irs-1 truncated mutated mouse (Irs-1−/−) with growth retardation and subcutaneous adipocyte atrophy. Irs-1−/− mice exhibited mild insulin resistance, as demonstrat-ed by the insulin tolerance test. Phosphatidylino-sitol 3-kinase (PI3K) activity and phosphorylated Protein Kinase B (PKB/AKT) expression were elevated in liver, skeletal muscle, and subcu-taneous adipocytes in Irs-1 deficiency. In addition, the expression of IRS-2 and its phosphorylated version were clearly elevated in liver and skeletal muscle. With miRNA microarray analysis, we found miR-33 was down-regulated in bone marrow stromal cells (BMSCs) of Irs-1−/− mice, while its target gene Irs-2 was up-regulated in vitro studies. In addition, miR-33 was down-regulated in the presence of Irs-1 and which was up-regulated in fasting status. What’s more, miR-33 restored its expression in re-feeding status. Meanwhile, miR-33 levels decreased and Irs-2 levels increased in liver, skeletal muscle, and subcutaneous adipocytes of Irs-1−/− mice. In primary cultured liver cells transfected with an miR-33 inhibitor, the expression of IRS-2, PI3K, and phosphorylated-AKT (p-AKT) increased while the opposite results were observed in the presence of an miR-33 mimic. Therefore, decreased miR-33 levels can up-regulate IRS-2 expression, which appears to compensate for the defects of the insulin signaling pathway in Irs-1 deficient mice.

A Generalized CNN Model with Automatic Hyperparameter Tuning for Millimeter Wave Channel Prediction

Yue, Chengfang,Tang, Hui,Yang, Jun,Chai, Li 한국통신학회 2023 Journal of communications and networks Vol.25 No.4

This paper focuses on millimeter wave (mmWave)channel prediction by machine learning (ML) methods. PreviousML-based mmWave channel predictors have limitations on re-quirements of the amount of training data, model generalizationability, robustness to noise, etc. In this paper, we propose aCNN model with a novel feature selection strategy for mmWavechannel prediction. Automatic hyperparameter tuning (AHT)algorithms are embedded in the training process to iterativelyoptimize the predictive performance of the proposed CNN. Thediversification strategy is leveraged to enhance the robustness ofthe AHT procedure against different communication scenarios. To improve the generalization ability of the prediction model, theinput features are designed to capture the correlation betweenthe physical environment and the channel characteristics. Inparallel, the Cartesian coordinates of the transmitter (Tx) and re-ceiver (Rx) are transformed into polar ones to reduce the model’ssensitivity to coordinate noise. Numerical results demonstrate theeffectiveness of the proposed CNN model in predicting mmWavechannel characteristics in various communication scenarios.

All-trans-retinoic Acid Promotes Iodine Uptake Via Up-regulating the Sodium Iodide Symporter in Medullary Thyroid Cancer Stem Cells

Tang, Min,Hou, Yan-Li,Kang, Qiang-Qiang,Chen, Xing-Yue,Duan, Li-Qun,Shu, Jin,Li, Shao-Lin,Hu, Xiao-Li,Peng, Zhi-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Recently, the main therapy of medullary thyroid cancer (MTC) is surgical, but by which way there is a poor prognosis with a mean survival of only 5 years. In some cases, some researchers found that it is the medullary thyroid cancer stem cells (MTCSCs) that cause metastasis and recurrence. This study aimed to eradicate MTCSCs through administration of all-trans-retinoic acid (ATRA). Here we demonstrate that MTCSCs possess stemlike properties in serum-free medium. The ABCG2, OCT4 and sodium iodide symporter (NIS) were changed by ATRA. Additionally, we found that ATRA can increase the expression of NIS in vivo. All the data suggested that ATRA could increase the iodine uptake of MTCSCs through NIS.

Facile Synthesis and Properties of Aqueous CdTe Quantum Dots for High-Sensitive Copper (II) Ion Detection

Yue Zheng,Hengshan Tang,Xiaoyu Wang,Ya Di,Kunling Lu,Jidong Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.12

The synthesis of aqueous MPA-capped CdTe quantum dots (QDs) via a facile one-pot route was developed. The particle size, optical properties and crystal structure of the as-synthesis QDs were investigated by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), UV-vis absorption and photoluminescence (PL) spectra, respectively. Meanwhile, the effect of reaction conditions, including reaction time, pH and the quantity of sodium citrate (SC) on the growth of CdTe QDs were discussed. Then, the obtained CdTe QDs were successfully used for the detection of trace Cu2+ with high sensitivity and excellent selectivity. The fluorescence intensity of CdTe QDs with Cu2+ concentration showed a linear relationship in the range from 1 x 10 -7 mol/L to 1 x 10 -6 mol/L. The correlation coefficient (R) is 0.9980 and limit of detection (LOD) is 1.0 x 10 -8 mol/L. Moreover, the concentration of Cu2+ in tap water samples was determined based on this sensing system and the recovery test was satisfactory.

Critical effects of long non-coding RNA on fibrosis diseases

Yue Zhang,Gang Luo,Yi Zhang,Mengjie Zhang,Jian Zhou,Weiwu Gao,Xiuyun Xuan,Xia Yang,Di Yang,Zhiqiang Tian,Bing Ni,Jun Tang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The expression or dysfunction of long non-coding RNAs (lncRNAs) is closely related to various hereditary diseases, autoimmune diseases, metabolic diseases and tumors. LncRNAs were also recently recognized as functional regulators of fibrosis, which is a secondary process in many of these diseases and a primary pathology in fibrosis diseases. We review the latest findings on lncRNAs in fibrosis diseases of the liver, myocardium, kidney, lung and peritoneum. We also discuss the potential of diseaserelated lncRNAs as therapeutic targets for the clinical treatment of human fibrosis diseases.

Synthesis of (Imido)vanadium(V) Complexes Containing 8-(2,6-Dimethylanilide)-5,6,7-trihydroquinoline Ligands: Highly Active Catalyst Precursors for Ethylene Dimerization

Tang, Xiao-Yan,Igarashi, Atsushi,Sun, Wen-Hua,Inagaki, Akiko,Liu, Jingyu,Zhang, Wenjuan,Li, Yue-Sheng,Nomura, Kotohiro American Chemical Society 2014 Organometallics Vol.33 No.4

<P>A series of (imido)vanadium(V) dichloride complexes containing 8-(2,6-dimethylanilide)-5,6,7-trihydroquinoline ligands of the type V(NR)Cl<SUB>2</SUB>[8-(2,6-Me<SUB>2</SUB>C<SUB>6</SUB>H<SUB>3</SUB>)N(C<SUB>9</SUB>H<SUB>10</SUB>N)] (R = Ad (<B>3</B>), 2-MeC<SUB>6</SUB>H<SUB>4</SUB> (<B>4</B>), 2,6-Me<SUB>2</SUB>C<SUB>6</SUB>H<SUB>3</SUB> (Ar, <B>5</B>)) have been prepared and identified, and their structures have been determined by X-ray crystallographic analysis. The ethylene dimerization catalyst generated from complex <B>3</B> upon treatment with an excess amount of MAO exhibited remarkable catalytic activities (e.g. TOF = 9600000 h<SUP>–1</SUP> (2670 s<SUP>–1</SUP>), Al/V = 4000 (molar ratio)), affording 1-butene as the major product (95.0–99.4%). The activities of <B>3</B> and <B>4</B> were higher than those exhibited by the corresponding 2-(anilide)methylpyridine analogues; <B>3</B> showed higher 1-butene selectivity than the others and the activity did not decrease remarkably at 50 °C. Complex <B>5</B> afforded a mixture of polymer and oligomers with low activities, suggesting that a fine tuning of both the imido and the anionic donor ligands plays an essential role in this catalysis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orgnd7/2014/orgnd7.2014.33.issue-4/om401119y/production/images/medium/om-2013-01119y_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/om401119y'>ACS Electronic Supporting Info</A></P>

Patient and Care Delays of Breast Cancer in China

Yue-Lin Li,Ya-Chao Qin,Lu-Ying Tang,Yu-Huang Liao,Wei Zhang,Xiao-Ming Xie,Qiang Liu,Ying Lin,Ze-Fang Ren 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3

Purpose This study differentiates patient and care delays of breast cancer and explores the related factors as well as the associations with the prognosis in Guangzhou, a southern city of China. Materials and Methods A cohort of female incident breast cancer patients (n=1,551) was recruited from October 2008 to March 2012 and followed up until January 1, 2016 (n=1,374) in the affiliated hospitals of Sun Yat-sen University. The factors associated with patient and care delays were analyzed with multivariable logistic models. Cox proportional hazards regression models were constructed to estimate the impacts of the delays on the prognosis. Results There were 40.4% patient delay (! 3 months) and 15.5% care delay (! 1 month). The patient delay, but not the care delay, was significantly related to the clinical stage and consequently worsened the prognosis of breast cancer (hazard ratio, 1.45; 95% confidence interval, 1.09 to 1.91 for progression-free survival). The factors related to an increased patient delay included premenopausal status, history of benign breast disease, and less physical examination. Conclusion Patient delay was the main type of delay in Guangzhou and resulted in higher clinical stage and poor prognosis of breast cancer. Screening for breast cancer among premenopausal women may be an effective way to reduce this delay.