Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang,Yajun Wang,Zhongkui Xie,Ruoyu Wang,Zhihong Guo,Yuhui He 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.2

The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV- infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been de- veloped to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65°C and 60°C for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction as- says. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification

Yubao Zhang,Zhongkui Xie,John D Fletcher,Yajun Wang,Ruoyu Wang,Zhihong Guo,Yuhui He 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.1

Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

Antioxidant capacity, flavor and physicochemical properties of FH06 functional beverage fermented by lactic acid bacteria: a promising method to improve antioxidant activity and flavor of plant functional beverage

Yan Xian-Tao,Zhang Ziqi,Wang Yubao,Zhang Wenmiao,Zhang Longfei,Liu Yang,Chen Dawei,Wang Wenqiong,Ma Wenlong,Qian Jian-Ya,Gu Ruixia 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-

The ability of natural plants to treat chronic diseases is closely related to their antioxidant function. Lactic acid bacteria (LAB) fermentation is an effective way to improve the nutritional value, biological activity and flavor of food. This study investigated the pH, titratable acidity, total polysaccharide, total flavone, total saponin, total polyphenol, and antioxidant activity of the FH06 beverage before and after probiotic fermentation. Results: After fermentation, FH06 had lower contents of total polysaccharides, total flavonoids, total saponins and total polyphenols but higher titratable acidity. The antioxidant activity was tested by total antioxidant capacity (FRAP method) and DPPH· scavenging ability. The FRAP value significantly increased after fermentation (P < 0.05), and the maximum increase was observed for Lactobacillus fermentum grx08 at 25.87%. For DPPH· scavenging ability, the value of all fermentations decreased, and L. fermentum grx08 had the smallest reduction at 2.21% (P < 0.05). The results of GC–MS and sensory analysis showed that fermentation eliminated bad flavors, such as grass, cassia and bitterness, and highlighted the fruit aroma and soft sour taste. Conclusion: The FRAP value and sensory flavor of FH06 fermentation by L. fermentum grx08 were significantly improved, indicating its great potential as a functional food with both strong antioxidant activity and good flavor.

Adaptive Neural Network Control of a 2-DOF Helicopter System with Input Saturation

Jian Zhang,Zhijia Zhao,Yubao Yang,홍금식 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.1

This paper investigates an adaptive neural network control strategy for a two-degree-of-freedom helicopter system with input saturation and unknown external disturbances. Firstly, the radial basis function neural network is used to compensate the uncertainty and input saturation error of the system. Furthermore, a disturbance observer is designed to deal with complex disturbances composed of unknown disturbances and neural network errors. By constructing and analyzing the Lyapunov function, the stability of the helicopter system is strictly guaranteed. Finally, the numerical simulations and experiments conducted on the Quanser laboratory platform reveal that the proposed control strategy is suitable and effective.

Drug distribution management system based on IoT

Zeliang Liu,Chunmei Zhang,Hui Peng,Qin Xu,Yubao Gao 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.2

In hospitals and pharmacies, the distribution of medicines is an important part. Any mistakes, misses, fake medicines and expired medicines can cause medical accidents. With the widespread application of the Internet of Things technology (IoT), traditional drug distribution methods need to be upgraded. This article proposes a drug distribution management scheme based on the Internet of Things technology. In the production of drugs, a flexible RFID tag was printed on the packaging box, which stored a series of information such as drug name, dosage, raw materials, efficacy, production date, expiration date, and manufacturer. The use of a drug distribution management system combined with RFID readers can identify drug information and effectively prevent the occurrence of erroneous, missed, counterfeit, and expired drugs. It can also improve management efficiency, reduce management costs, and control management risks. Through the circuit design and software system development, the test results show that this solution is effective and feasible, the proposed method can achieve the expected results.

Improving the Color Gamut of a Liquid-crystal Display by Using a Bandpass Filter

Yan Sun,Chi Zhang,Yanling Yang,Hongmei Ma,Yubao Sun 한국광학회 2019 Current Optics and Photonics Vol.3 No.6

To improve the color gamut of a liquid-crystal display (LCD), we propose a bandpass filter that is added to the backlight unit to optimize the backlight spectrum. The bandpass filter can only transmit red, green and blue light in the visible range, while reflecting the unwanted light. We study the optical properties of the bandpass filter using the transfer-matrix method, and the effect of the bandpass filter on the color gamuts of LCDs is also investigated. When a bandpass filter based on a 5-layer configuration comprising low and high refractive indices ((HL) 2 H) is used in phosphor-converted white-light-emitting diode (pc-WLED), K 2 SiF 6 :Mn 4+ (KSF-LED), and quantum-dot (QD) backlights, the color gamuts of the LCDs improve from 72% to 95.3% of NTSC, from 92% to 106.7% of NTSC, and from 104.3% to 112.2% of NTSC respectively. When the incident angle of light increases to 30°, the color gamuts of LCDs with pc-WLED and KSF-LED backlights decrease by 2.9% and 1% respectively. For the QD backlight, the color gamut almost does not change. When the (HL) 2 H structure is coated on the diffusion film, the color gamut can be improved to 92.6% of NTSC (pc-WLED), 105.6% of NTSC (KSF-LED), and 111.9% of NTSC (QD). The diffusion film has no obvious effect on the color gamut. The results have an important potential application in wide-color-gamut LCDs.

v-PATHS OF ARCS IN REGULAR MULTIPARTITE TOURNAMENTS

Yao, Tianxing,Guo, Yubao,Zhang, Kemin Korean Mathematical Society 1999 대한수학회보 Vol.36 No.2

A v-path of an arc xy in a multipartite tournament T is an oriented oath in T-y which starts at x such that y does not dominate and end vertex of the path. We show that if T is a regular n-partite (n$\geq$7) tournament, then every arc of T has a v-path of length m for all m satisfying 2$\leq$m$\leq$n-2. Our result extends the corresponding result for regular tournaments, due to Alspach, Reid and Roselle [2] in 1974, to regular multipartite tournaments.

Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

Huawei Li,Jifei Yang,Dengke Bao,Jie Hou,Yubao Zhi,Yanyan Yang,Pengchao Ji,Enmin Zhou,Songlin Qiao,Gaiping Zhang 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.