Kinesin Family Member 11 Enhances the Self-Renewal Ability of Breast Cancer Cells by Participating in the Wnt/β-Catenin Pathway

Yuan-yuan Pei,Gao-chi Li,Jian Ran,Xin-hong Wan,Feng-xiang Wei,Lan Wang 한국유방암학회 2019 Journal of breast cancer Vol.22 No.4

Purpose: Our previous studies have shown that kinesin family member 11 (KIF11) is markedly overexpressed in human breast cancer cells or tissues and positively correlated with distant metastasis and prognosis in patients with breast cancer, suggesting an important role in the regulation of cancer stem cells. Herein, we examined the role of KIF11 in breast cancer stem cells. Methods: In the current study, we validated our previous findings through analysis of data collected in The Cancer Genome Atlas. Endogenous KIF11 was stably silenced in MCF-7 and SKBR-3 cells. Flow cytometry was used to measure the proportion of side-population (SP) cells. Mammosphere culture and tumor implantation experiments in immunodeficient mice were used to assess the self-renewal ability of breast cancer cells. Real-time polymerase chain reaction, western blot, immunofluorescence staining, luciferase reporter assays and Wnt agonist treatment were conducted to investigate the signaling pathways regulated by KIF11. Results: We found that the expression level of KIF11 was positively correlated with stem cell-enrichment genes. The proportion of SP cells was significantly reduced in KIF11-silenced cells. Silencing endogenous KIF11 not only reduced the size and number of mammospheres in vitro, but also reduced the ability of breast cancer cells to form tumors in mice. Simultaneously, we found that KIF11 was involved in regulating the activation of the Wnt/β-catenin signaling pathway. Conclusion: Endogenous KIF11 enhances the self-renewal of breast cancer cells by activating the Wnt/β-catenin signaling pathway, thereby enhancing the characteristics of breast cancer stem cells.

GCP(Genistein Combined Polysaccharide) : A Fermented Soybean Isoflavone Health Food with Multi-Physical Activities and Their Applications in Cancer Prevention and Life-Style Related Diseases

Yuan, Lan,Wagatsuma, Chihiro,Miura, Takehito,Kim, Jung-Hwan,Surh, Young-Joon 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.4

Risk of Hepatitis B Virus (HBV) Reactivation in HBsAg-Negative, Anti-HBc-Negative Patients Receiving Rituximab for Autoimmune Diseases in HBV Endemic Areas

Lan Ting-Yuan,Lin Yen-Chun,Tseng Tai-Chung,Yang Hung-Chih,Kao Jui-Hung,Cheng Chiao-Feng,Lee Tai-Ju,Huang Shang-Chin,Lu Cheng-Hsun,Li Ko-Jen,Hsieh Song-Chou 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.2

Background/Aims: Rituximab is known to be associated with high hepatitis B virus (HBV) reactivation rate in patients with resolved HBV infection and hematologic malignancy. However, data regarding HBV reactivation (HBVr) in rheumatic patients receiving rituximab is limited. To assess the HBVr rate in hepatitis B surface antigen (HBsAg)-negative patients receiving rituximab for autoimmune diseases in a large real-world cohort. Methods: From March 2006 to December 2019, 900 patients with negative HBsAg receiving at least one cycle of rituximab for autoimmune diseases in a tertiary medical center in Taiwan were retrospectively reviewed. Clinical outcome and factors associated with HBVr were analyzed. Results: After a median follow-up period of 3.3 years, 21 patients developed HBVr, among whom 17 patients were positive for hepatitis B core antibody (anti-HBc) and four were negative. Thirteen patients had clinical hepatitis flare, while eight patients had HBsAg seroreversion without hepatitis. Old age, anti-HBc positivity, undetectable serum hepatitis B surface antibody level at rituximab initiation and a higher average rituximab dose were associated with a higher HBVr rate. There was no significant difference in the HBVr risk between rheumatoid arthritis and other autoimmune diseases. Among anti-HBc-negative patients, subjects without HBV vaccination at birth had an increased risk of HBVr (4/368, 1.1%) compared with those who received vaccination (0/126, 0%). Conclusions: In HBV endemic areas where occult HBV is prevalent, anti-HBc-negative patients, may still be at risk for HBVr after rituximab exposure. HBVr may still be considered in HBsAgnegative patients developing abnormal liver function after rituximab exposure, even in patients with negative anti-HBc.

유무선 통합 환경에서 QoS 향상을 위한 M-QDMR 알고리즘

원미란(Yuan Mei-Lan),조용환(Cho Yong-Hwan) 한국콘텐츠학회 2003 한국콘텐츠학회 종합학술대회 논문집 Vol.1 No.2

현재 정보통신은 유무선 통합의 방향으로 나아가고 있으며, 유무선 통합에서 QoS관리가 가장 걸림돌이 되고 있다. 본 논문에서는 유무선 통합 환경에서 QoS 향상을 위한 M-QDMR 알고리즘을 제안한다. 시뮬레이션 결과, M-QDMR 알고리즘은 가장 절약한 대역폭으로 지연이 비교적 작은 멀티캐스트 트리를 구성할 수 있음을 인증하였다. In this paper, we propose the M-QDMR algorithm for improvement QoS in the wire/wireless integrated environment. Through simulations and comparing to another multicast algorithm, we reach a conclusion is that M-QDMR can simply and dynamically adjusts the construction of multicast tree with little delay and the most reducible bandwidth resources.

Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

Phytoestrogen Intake and Risk of Ovarian Cancer: a Meta-Analysis of 10 Observational Studies

Qu, Xin-Lan,Fang, Yuan,Zhang, Ming,Zhang, Yuan-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: Epidemiology studies have shown an inconclusive relationship between phytoestrogen intake and ovarian cancer risk and there have been no relevant meta-analyses directly regarding this topic. The purpose of the present meta-analysis was therefore to investigate any association between phytoestrogen intake and ovarian cancer in detail. Materials and Methods: We conducted a search of PubMed, EMBASE, EBSCO, the Cochrane Library, CNKI and Chinese Biomedical Database (up to April 2014) using common keywords for studies that focused on phytoestrogen and ovarian cancer risk. Study-specific risk estimates (RRs) were pooled using fixed effect or random-effect models. Results: Ten epidemiologic studies were finally included in the meta-analysis. The total results indicated higher phytoestrogen intake was associated with a reduced ovarian cancer risk (RR, 0.70; 95%CI: 0.56-0.87). The association was similar in sensitivity analysis. Meta regression analysis demonstrated sources and possibly types and regions as heterogeneous factors. Subgroup analysis of types, sources and regions showed that isoflavones (RR: 0.63; 95%CI: 0.46, 0.86), soy foods (RR: 0.51; 95%CI: 0.39, 0.68) and an Asian diet (RR: 0.48; 95%CI: 0.37, 0.63) intake could reduce the incidence of ovarian cancer. Conclusions: Our findings show possible protection by phytoestrogens against ovarian cancer. We emphasize specific phytoestrogens from soy foods, but not all could reduce the risk. The habit of plentiful phytoestrogen intake by Asians is worthy to recommendation. However, we still need additional larger well designed observational studies to fully characterize underlying associations.

Clinicopathological Significance of Expression of Tspan-1, Jab1 and p27 in Human Hepatocellular Carcinoma

Li Chen,Daiyue Yuan,Gui-lan Wang,You Wang,Yuan-Yuan Wu,Jianwei Zhu 대한의학회 2010 Journal of Korean medical science Vol.25 No.10

The aim of this study was to investigate the expression of Tspan-1, Jab1 and p27 in human hepatocellular carcinoma (HCC) and their clinicopathological significance. The expression of Tspan-1, Jab1 and p27 was detected in HCC tissues, the tissues around cancer (76 cases),and the normal tissues around the liver hemangiomas (10 cases). The overexpression of Tspan-1 and Jab1 was found in HCC tissues, positively correlated with clinical stage and negatively correlated with survival rate. The expression of p27 was found inversely linked to which of Tspan-1 and Jab1. In conclusion, the expression of Tspan-1, Jab1 and p27 is significantly associated with development of HCC. Overexpression of Tspan-1 and Jab1suggests poor prognosis but overexpression of p27 may expect good prognosis for patients with HCC.

The Improvement of the Opacity and Printing Strength of Fancy Paper Overlaid Plywood

LAN-SHENG KUO,YUAN-SHING PERNG,EUGENE I-CHEN WANG,CHEN-FA YEN,TSUEN-HAN KAO 한국펄프·종이공학회 2006 한국펄프종이학회 기타 간행물 Vol.- No.-

The purpose of this study is to investigate the opacity and printing strength of MG paper overlaid plywood. The printing strength of ink on MG paper can be evaluated effectively by a formula E<SUP>*2</SUP> = [(L<SUP>*</SUP>)<SUP>2</SUP> + (a<SUP>*</SUP>)<SUP>2</SUP> + (b<SUP>*</SUP>)<SUP>2</SUP>]<SUP>1/2</SUP> that we proposed. Higher E value indicates good printing strength of ink-on-paper. We also assess the real color of translucent printed MG paper with a formula CIE △E<SUP>*</SUP> (color difference between a pile of same paper to be opaque and fancy paper laminated board). In addition, the color difference on paper surface caused by the color of wood-based board (bottom) can be evaluated by a formula of Pc. No. Generally, an acceptable appearance quality of fancy boards is △E<SUP>*</SUP> < 2.0 and small Pc.No. value. The experimental results showed that Japan-made MG papers ?J1, J2 and J3 have better printing strength and gloss than that of Taiwan-made paper (T1). The reason for this was that Taiwan-made paper has poor printing strength and low gloss, which might be correlated to the fiber compositions in paper. Higher printing strength can be seen for short fiber containing handsheets when comparing to that of handsheets. Nonetheless, low-freeness sheets gives better printing strength than that of high-freeness sheets. High-opacity MG paper gives good opacifying effect to the fancy paper laminated wood-based boards. Comparing the surface color of 2 kinds of fancy paper laminated boards, paperboard T1 laminated with high-opacity fancy paper showed slight color difference. The same results can be seen for ?? g/㎡ handsheets. Higher-opacity Acacia and Eucalyptus bleached sulfate pulps (short fiber) gives higher opacifying effect on the plywood when comparing to Northan pine and Radiata pine sulfate pulps(long fiber). The former ones also showed small color differences when comparing the color differences between the color of fancy paper and laminated paper board. Additionally, the color of bottom plywood can’t be shown through for the highopacify surface paper adhered to. Besides, the PC No of the base paper laminated board is small as well. Apparently, we can add colorants to the binders for the manufacture of various handsheets (30 g/㎡) with various pulp mix ratios to increase the opacity of paperboards to certain extents. When we using yellow and brown binders in paper laminated board, the color difference between Acacia and Eucalyptus handsheets overlaid boards decreasing to 2.0 (acceptable △E<SUP>*</SUP>< 2.0, hard to discern), but not much improvement for Northern and Radiata pines. Definitely, show-through defects can be discernible for lower opacity papers. In general, admirable printing strength of fancy paper by which glued to plywood can be made with high-opacity paper and colored binders techniques.

Additive Role of the Vestibular End Organ and Baroreceptors on the Regulation of Blood Pressure in Rats

Lan, Yan,Yang, Yan-Zhao,Jiang, Xian,Li, Li-Wei,Jin, Guang-Shi,Kim, Min Sun,Park, Byung Rim,Jin, Yuan-Zhe The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Contribution of the vestibular end organ to regulation of arterial pressure was quantitatively compared with the role of baroreceptors in terms of baroreflex sensitivity and c-Fos protein expression in the rostral ventrolateral medulla (RVLM). Baroreflex sensitivity and c-Fos protein expression in the RVLM were measured in conscious rats that had undergone bilateral labyrinthectomy (BL) and/or baroreceptor unloading. BL attenuated baroreflex sensitivity during intravenous infusion of sodium nitroprusside (SNP), but did not significantly affect the sensitivity following infusion of phenylephrine (PE). Baroreflex sensitivity became positive following sinoaortic denervation (SAD) during infusion of PE and attenuated sensitivity during infusion of SNP. Baroreflex sensitivity also became positive following double ablation (BL+SAD) during infusion of PE, and attenuated sensitivity during infusion of SNP. c-Fos protein expression increased significantly in the RVLM in the sham group after SNP administration. However, the BL, SAD, and SAD+BL groups showed significant decreases in c-Fos protein expression compared with that in the sham group. The SAD group showed more reduced c-Fos protein expression than that in the BL group, and the SAD+BL group showed less expression than that in the SAD group. These results suggest that the vestibular system cooperates with baroreceptors to maintain arterial pressure during hypotension but that baroreceptors regulate arterial pressure during both hypotension and hypertension. Additionally, afferent signals for maintaining blood pressure from the vestibular end organs and the baroreceptors may be integrated in the RVLM.

Analysis of the Baroreceptor and Vestibular Receptor Inputs in the Rostral Ventrolateral Medulla following Hypotension in Conscious Rats

Lan, Yan,Lu, Huan-Jun,Jiang, Xian,Li, Li-Wei,Yang, Yan-Zhao,Jin, Guang-Shi,Park, Joo Young,Kim, Min Sun,Park, Byung Rim,Jin, Yuan-Zhe The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.2

Input signals originating from baroreceptors and vestibular receptors are integrated in the rostral ventrolateral medulla (RVLM) to maintain blood pressure during postural movement. The contribution of baroreceptors and vestibular receptors in the maintenance of blood pressure following hypotension were quantitatively analyzed by measuring phosphorylated extracellular regulated protein kinase (pERK) expression and glutamate release in the RVLM. The expression of pERK and glutamate release in the RVLM were measured in conscious rats that had undergone bilateral labyrinthectomy (BL) and/or sinoaortic denervation (SAD) following hypotension induced by a sodium nitroprusside (SNP) infusion. The expression of pERK was significantly increased in the RVLM in the control group following SNP infusion, and expression peaked 10 min after SNP infusion. The number of pERK positive neurons increased following SNP infusion in BL, SAD, and BL+SAD groups, although the increase was smaller than seen in the control group. The SAD group showed a relatively higher reduction in pERK expression when compared with the BL group. The level of glutamate release was significantly increased in the RVLM in control, BL, SAD groups following SNP infusion, and this peaked 10 min after SNP infusion. The SAD group showed a relatively higher reduction in glutamate release when compared with the BL group. These results suggest that the baroreceptors are more powerful in pERK expression and glutamate release in the RVLM following hypotension than the vestibular receptors, but the vestibular receptors still have an important role in the RVLM.