Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Jiansheng Wei,Liangyan Liu,Xiaolong Yuan,Dong Wang,Xinyue Wang,Wei Bi,Yan Yang,Yi Wang 한국균학회 2023 Mycobiology Vol.51 No.5

Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang. To analyzehispidin biosynthesis in S. sanghuang, the transcriptomes of hispidin-producing and non-producingS. sanghuang were determined by Illumina sequencing. Five PKSs were identifiedusing genome annotation. Comparative analysis with the reference transcriptome showedthat two PKSs (ShPKS3 and ShPKS4) had low expression levels in four types of media. Thegene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies(LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KSdomain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceaeform a unique monophyletic clade closely related to the clade containing Agaricales hispidinsynthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuanginvolved in hispidin biosynthesis.

Ginsenoside Rh2 inhibiting HCT116 colon cancer cell proliferation through blocking PDZ-binding kinase/T-LAK cell-originated protein kinase

Yang, Jianjun,Yuan, Donghong,Xing, Tongchao,Su, Hongli,Zhang, Shengjun,Wen, Jiansheng,Bai, Qiqiang,Dang, Dongmei The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4

Background: Ginsenoside Rh2 (GRh2) is the main bioactive component in American ginseng, a commonly used herb, and its antitumor activity had been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in HCT116 colorectal cancer cells. Methods: We examined the effect of GRh2 on HCT116 cells ex vivo. Next, we performed in vitro binding assay and in vitro kinase assay to search for the target of GRh2. Furthermore, we elucidated the underlying molecular mechanisms for the antitumor effect of GRh2 ex vivo and in vivo. Results: The results of our in vitro studies indicated that GRh2 can directly bind with PBK/TOPK and GRh2 also can directly inhibit PBK/TOPK activity. Ex vivo studies showed that GRh2 significantly induced cell death in HCT116 colorectal cancer cells. Further mechanistic study demonstrated that these compounds inhibited the phosphorylation levels of the extracellular regulated protein kinases 1/2 (ERK1/2) and (H3) in HCT116 colorectal cancer cells. In vivo studies showed GRh2 inhibited the growth of xenograft tumors of HCT116 cells and inhibited the phosphorylation levels of the extracellular regulated protein kinases 1/2 and histone H3. Conclusion: The results indicate that GRh2 exerts promising antitumor effect that is specific to human HCT116 colorectal cancer cells through inhibiting the activity of PBK/TOPK.

Semianalytical Approach to the Inverse Fourier Transform and Its Application in Evaluating Lightning Horizontal Electric Field

Boyuan Zhang,Jiansheng Yuan,Jun Zou,Jaebok Lee,Mun-No Ju [Institute of Electrical and Electronics Engineers 2016 IEEE transactions on electromagnetic compatibility Vol.58 No.2

<P>A semianalytical inverse Fourier transform, which can evaluate the time response at arbitrary time, is proposed. The basic idea is fitting the response in frequency domain by a sum of piecewise cubic spline functions with an adaptive sampling strategy, and the response in time domain is evaluated with the help of the moment functions to avoid calculating the highly oscillatory integral. A recursive algorithm is derived, which can efficiently calculate the moment functions with different ranks. As an application of the proposed approach, the underground horizontal electric field of a lightning channel is evaluated in the time domain. In comparison with the results in the published literature, the numerical examples show that the proposed approach can save the computational time at least 50 times compared to the one using a conventional inverse Fourier transform technique. The proposed approach, in fact, can be generalized to apply to other scenarios when the inverse Fourier transform is involved.</P>

Calculation of Ion-Flow Field of HVdc Transmission Lines in the Presence of Wind Using Finite Element-Finite Difference Combined Method With Domain Decomposition

Ji Qiao,Jun Zou,Jiansheng Yuan,Lee, J. B.,Munno Ju IEEE 2016 IEEE transactions on magnetics Vol.52 No.3

<P>A combined method adopting the domain decomposition is proposed for analyzing the ion-flow field of high-voltage direct current transmission lines, including the effect of the transverse wind. The upstream finite-element method is used with a dense triangle mesh in the vicinity of bundle conductors to guarantee the accuracy. The upstream finite-difference method is proposed and the larger uniform quadrilateral grid is applied to simulate the ion-flow field in the rest of the region with a satisfactory precision. The calculation process of the Poisson equation is iterated with the Dirichlet-Neumann algorithm to coordinate the solution between adjacent subdomains. The proposed approach improves the efficiency and remains stable with high wind speed. Finally, the influence of wind on the ion-flow field is analyzed and calculations are in good agreement with experimental data and results in the previous literature.</P>

Method of local characteristics for calculating electric field and ion current of HVDC transmission lines with transverse wind

Qiao, Ji,Zou, Jun,Yuan, Jiansheng,Lee, Jaebok,Ju, Mun-no THE INSTITUTION OF ENGINEERING AND TECHNOLOGY 2017 IET GENERATION TRANSMISSION AND DISTRIBUTION Vol.11 No.4

<P>The electric field and ion flow are significantly influenced by the transverse wind around the high-voltage direct current (HVDC) transmission lines. In this study, a new method using local characteristics is proposed to analyse the effect of wind. The present method, called method of local characteristic, updates the ion density based on the local characteristic curve in each second-order finite element method (FEM) element without Deutsch assumption. The defining equation of the characteristics is able to take into account the directional character of the information propagation in convective transport and has a better performance to satisfy the conservation law. The present approach is stable and efficient even in the presence of high-speed transverse wind. Calculations show good agreement with the measurement values of the reduced-scale unipolar model and the full-scale bipolar test line. Finally, the influence of wind on the electric field and ion current is discussed.</P>

Electrostatic Field Calculation and Structure Optimization of New Shield Ring of 1000 kV Nan-Jing Loop Transmission Line

Zhi Li,Zhang Yujiao,Xiang Guanteng,Yuan Jiansheng,Jiang Lan 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.3

Considering the frequent breakage of the hard jumper shield ring in the strain tower of ultra-high voltage alternating current 1000 kV Nan-Jing Transmission Line, the theoretical and experimental studies on new shield ring were carried out in terms of mechanical properties; however, due to the structural changes of shield ring, the fi eld characteristics should be calculated and analyzed. In accordance with the theory that there is no electric field in a metal conductor under electrostatic equilibrium, if there is any metal conductor in the 3D model of the computational domain, there will be no need to subdivide the metal conductor. In this paper, a 3D model load applying method with metal conductors was proposed to calculate the field intensity of Tuned mass damper (TMD) shield ring after improving the mechanical properties of the strain tower, which saved the total number of mesh subdivisions by 48.02%, reduced the computational time by 76.47%, and increased the computational effi ciency of 3D numerical electric field. The calculation results validated the correctness and effectiveness of this method. The new TMD shield ring with the field intensity meeting the relevant requirements was adopted in 1000 kV Nan-Jing Loop Transmission Line in December 2016, which has been operating in good condition so far.