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Chiang, Ming-Lun,Chen, Hsi-Chia,Chen, Kun-Nan,Lin, Yu-Chun,Lin, Ya-Ting,Chen, Ming-Ju Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8
Two probiotic strains, Lactobacillus johnsonii x-1d-2 and Lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in RAW 267.4 macrophages in our previous study. These characteristics make L. johnsonii x-1d-2 and L. mucosae x-4w-1 good candidates for application in feed probiotics. In this study, soybeal meal, molasses and sodium acetate were selected to optimize the growth medium for cultivation of L. johnsonii x-1d-2 and L. mucosae x-4w-1. These two strains were then freeze-dried and mixed into the basal diet to feed the weaned piglets. The effects of L. johnsonii x-1d-2 and L. mucosae x-4w-1 on the growth performance and fecal microflora of weaned piglets were investigated. The results showed that the bacterial numbers of L. johnsonii x-1d-2 and L. mucosae x-4w-1 reached a maximum of 8.90 and 9.30 log CFU/mL, respectively, when growing in optimal medium consisting of 5.5% (wt/vol) soybean meal, 1.0% (wt/vol) molasses and 1.0% (wt/vol) sodium acetate. The medium cost was 96% lower than the commercial de Man, Rogosa and Sharpe medium. In a further feeding study, the weaned piglets fed basal diet supplemented with freeze-dried probiotic cultures exhibited higher (p<0.05) body weight gain, feed intake, and gain/feed ratio than weaned piglets fed basal diet. Probiotic feeding also increased the numbers of lactobacilli and decreased the numbers of E. coli in the feces of weaned piglets. This study demonstrates that L. johnsonii x-1d-2 and L. mucosae x-4w-1 have high potential to be used as feed additives in the pig industry.
Ming-Lun Chiang,Hsi-Chia Chen,Kun-Nan Chen,Yu-Chun Lin,Ya-Ting Lin,Ming-Ju Chen 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8
Two probiotic strains, Lactobacillus johnsonii x-1d-2 and Lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in RAW 267.4 macrophages in our previous study. These characteristics make L. johnsonii x-1d-2 and L. mucosae x-4w-1 good candidates for application in feed probiotics. In this study, soybeal meal, molasses and sodium acetate were selected to optimize the growth medium for cultivation of L. johnsonii x-1d-2 and L. mucosae x-4w-1. These two strains were then freeze-dried and mixed into the basal diet to feed the weaned piglets. The effects of L. johnsonii x-1d-2 and L. mucosae x-4w-1 on the growth performance and fecal microflora of weaned piglets were investigated. The results showed that the bacterial numbers of L. johnsonii x-1d-2 and L. mucosae x-4w-1 reached a maximum of 8.90 and 9.30 log CFU/mL, respectively, when growing in optimal medium consisting of 5.5% (wt/vol) soybean meal, 1.0% (wt/vol) molasses and 1.0% (wt/vol) sodium acetate. The medium cost was 96% lower than the commercial de Man, Rogosa and Sharpe medium. In a further feeding study, the weaned piglets fed basal diet supplemented with freeze-dried probiotic cultures exhibited higher (p<0.05) body weight gain, feed intake, and gain/feed ratio than weaned piglets fed basal diet. Probiotic feeding also increased the numbers of lactobacilli and decreased the numbers of E. coli in the feces of weaned piglets. This study demonstrates that L. johnsonii x-1d-2 and L. mucosae x-4w-1 have high potential to be used as feed additives in the pig industry.
Feasibility of using a rotating packed bed in preparing coupled ZnO/SnO2 photocatalysts
Chia-Chang Lin,Yu-Ju Chiang 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4
In this work, coupled ZnO/SnO2 photocatalysts were prepared in a rotating packed bed (RPB) via coprecipitation. The precursors of coupled ZnO/SnO2 photocatalysts were formed from solutions of zinc sulfate, tin tetrachloride and sodium hydroxide. The calcinations of these precursors yielded coupled ZnO/SnO2 photocatalysts. The effect of calcination temperature on the characteristics and photocatalytic activity of coupled ZnO/SnO2 photocatalysts was studied. The photocatalytic activity of coupled ZnO/SnO2 photocatalysts was evaluated using the photocatalytic decolorization of methylene blue. The experimental results reveal that coupled ZnO/SnO2 photocatalysts that were obtained by calcination at 600 8C for 10 h were the most efficient in decolorizing methylene blue.
( Sang-su Kim ),( Cheol Hong Kim ),( Ji Wook Kim ),( Hsi Chiang Kung ),( Tae Woo Park ),( Yu Som Shin ),( Ju Deok Kim ),( Siejeong Ryu ),( Wang-joon Kim ),( Yung Hyun Choi ),( Kyoung Seob Song ) 생화학분자생물학회 2017 BMB Reports Vol.50 No.10
CLB<sub>2.0</sub>, a constituent of PM, induces secretion of multiple cytokines and chemokines that regulate airway inflammation. Specifically, IL-6 upregulates CLB<sub>2.0</sub>-induced MUC5AC and MUC1 expression. Interestingly, of the tight junction proteins examined, claudin-1 expression was inhibited by CLB<sub>2.0</sub>. While the overexpression of claudin-1 decreased CLB<sub>2.0</sub>-induced MUC5AC expression, it increased the expression of the anti-inflammatory mucin, MUC1. CLB<sub>2.0</sub>-induced IL-6 secretion was mediated by ROS. The ROS scavenger N-acetylcysteine inhibited CLB<sub>2.0</sub>-induced IL-6 secretion, thereby decreasing the CLB<sub>2.0</sub>-induced MUC5AC expression, whereas CLB<sub>2.0</sub>-induced MUC1 expression increased. CLB<sub>2.0</sub> activated the ERK1/2 MAPK via a ROS-dependent pathway. ERK1/2 downregulated the claudin-1 and MUC1 expressions, whereas it dramatically increased CLB<sub>2.0</sub>-induced MUC5AC expression. These findings suggest that CLB<sub>2.0</sub>-induced ERK1/2 activation acts as a switch for regulating inflammatory conditions though a ROS-dependent pathway. Our data also suggest that secreted IL-6 regulates CLB<sub>2.0</sub>-induced MUC5AC and MUC1 expression via ROS-mediated downregulation of claudin-1 expression to maintain mucus homeostasis in the airway. [BMB Reports 2017; 50(10): 516-521]