칠복음(七福飮)이 Glucose Oxidase에 의해 손상(損傷)된 대뇌피질(大腦皮質) 신경세포(神經細胞)에 미치는 영향(影響)

최공한,박승택,류도곤,최민호,엄상섭,허진영,강성도,고정수,서의석,성은경,조남수,이춘우,황일택,선성규,류영수,Choi, Kong-Han,Park, Seung-Taeck,Ryu, Do-Gon,Choi, Min-Ho,Um, Sang-Sub,Hea, Jin-Young,Kang, Sung-Do,Go, Jeong-Soo,Sou, Eui-Suk,Sung, Yeun-Ky 대한동의생리학회 1999 동의생리학회지 Vol.14 No.2

As the average life span have been lengthened and the rate of senile population have been raised, chronic degenerative diseases incident to aging has been increased rapidly and become a social problem. With this social background, recently, the facts that oxygen radicals(OR) have toxic effects on Central Nervous System and Peripheral Nervous System and cause neuropathy such as Parkinson's Disease, Alzheimer Disease have been turned out, and accordingly lots of studies on the mechanism of the toxic effects of OR on nerves, the diseases caused by OR and the approaches to curing the diseases have been made. The purpose of this study is to examine the toxic effects caused by Glucose Oxidase(GO) and the effects of herbal extracts such as Chilbokyeum(CBY) on the treatment of the toxic effects. For this purpose, experiments with the cultured cell from the cerebrums of new born mice were done. The results of these experiments were as follows. 1. GO, a oxygen radical, decreased the survival rate of the cultured cells on NR assay and MTT assay 2. GO, a oxygen radical, increased lipid peroxidation and the amount of LDH. 3. CBY have efficacy of decreasing lipid peroxidation. 4. CBY have efficacy of decreasing the amount of LOH. From the above results, It is concluded that Chilbokyeum has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process. And Chilbokyeum is thought to have certain pharmacological effects on controlling over aging and treating Dementia. Further clinical study of this pharmacological effects of Chilbokyeum should be complemented.

석창포(石菖蒲) 전탕액(煎湯液)이 Glucose Oxidase에 의해 손상(損傷)된 대뇌피질(大腦皮質) 신경세포(神經細胞)에 미치는 영향(影響)

최공한,박승택,류도곤,최민호,허진영,강성도,고정수,양상철,성은경,조남수,이춘우,서의석,류영수,Choi, Kong-Han,Park, Seung-Taeck,Ryu, Do-Gon,Choi, Min-Ho,Hea, Jin-Young,Kang, Sung-Do,Go, Jeong-Soo,Yang, Sang-cheal,Sung, Yeun-Kyung,Cho, Nam-Su,L 대한동의생리학회 1999 동의생리학회지 Vol.14 No.2

The purpose of this study is to examine the toxic effects caused by Glucose Oxidase(GO) and the effects of herbal extracts such as Acori Rhizoma(AR) on the treatment of the toxic effects. For this purpose, experiments with the cultured cell from the cerebrums of new born mice were done. The results of these experiments were as follows. 1. GO, a oxygen radical, decreased the survival rate of the cultured cells on NR assay and MTT assay. 2. GO, a oxygen radical, decreased the amount of neurofilaments and total protein. 3. AR have efficacy of increasing the amount of neurofilament. 4. AR have efficacy of increasing the amount of total protein. From the above results, It is concluded that AR has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process. And AR is thought to have certain pharmacological effects on controlling over aging. Further clinical study of this pharmacological effects of AR should be complemented.

Low Molecular Weight Polyethylenimine-Mitochondrial Leader Peptide Conjugate for DNA Delivery to Mitochondria

Choi, Joon-Sig,Choi, Min-Ji,Go, Gyeong-Su,Rhee, Byoung-Doo,KimPak, Young-Mi,Bang, In-Seok,Lee, Min-Hyung Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.9

It has been found that a number of diseases are associated with mutations in the mitochondrial DNA. Therapeutic gene delivery to mitochondria has been suggested as a clinical option for these diseases. In this study, we developed a gene carrier to mitochondria by the conjugation of mitochondrial leader peptide (LP) to polyethylenimine (PEI). Mitochondrial LP conjugated PEI (PEI-LP) was synthesized with low molecular weight PEI (2,000 Da, PEI2K). Gel retardation assay showed that PEI2K-LP formed complexes at a 1.0/1 weight ratio. In addition, PEI2K-LP protected DNA from the enzymatic degradation for at least 60 min, while naked DNA was completely degraded within 20 min. PEI2K-LP was compared with LP conjugated high molecular weight PEI (25,000 Da, PEI25K) in terms of toxicity and delivery efficiency. MTT assay showed that PEI2K-LP had much lower cytotoxicity than PEI25K-LP to 293 cells. In addition, cell-free DNA delivery assay showed that PEI2K-LP delivered more DNA to mitochondria at a 1.8/1 weight ratio than naked DNA or PEI. This result suggests that PEI2K-LP may be useful for the development of mitochondrial gene therapy system with lower cytotoxicity.

BioSMACK: a Linux Live CD for genome-wide association analyses

( Chang Bum Hong ),( Young Jin Kim ),( Sang Hoon Moon ),( Young Ah Shin ),( Min Jin Go ),( Dong Joon Kim ),( Jong Young Lee ),( Yoon Shin Cho ) 생화학분자생물학회 2012 BMB Reports Vol.45 No.1

Recent advances in high-throughput genotyping technologies have enabled us to conduct a genome-wide association study (GWAS) on a large cohort. However, analyzing millions of single nucleotide polymorphisms (SNPs) is still a difficult task for researchers conducting a GWAS. Several difficulties such as compatibilities and dependencies are often encountered by researchers using analytical tools, during the installation of software. This is a huge obstacle to any research institute without computing facilities and specialists. Therefore, a proper research environment is an urgent need for researchers working on GWAS. We developed BioSMACK to provide a research environment for GWAS that requires no configuration and is easy to use. BioSMACK is based on the Ubuntu Live CD that offers a complete Linux-based operating system environment without installation. Moreover, we provide users with a GWAS manual consisting of a series of guidelines for GWAS and useful examples. BioSMACK is freely available at http://ksnp.cdc. go.kr/biosmack. [BMB reports 2012; 45(1): 44-46]

관상동맥색전증에 의한 급성심근경색증 4예

김민근,주혜원,강경필,최병돈,박민석,변영섭,고충원,이건주,김병옥 인제대학교 2006 仁濟醫學 Vol.27 No.-

Acute myocardial infarction(AMI) due to coronary embolism can occur in patients with normal coronary artery. Coronary embolism is believed to be a cause of acute myocardial infarction when emergent coronary angiography shows perfectly normal coronary trees, except for the finding of coronary artery occlusion. In this report, we describe four patients with AMI due to coronary embolism who were successfully treated using a thrombus aspiration device and anticoagulation, and those patients had intracardial thrombus arising from the left atrium due to chronic atrial fibrillation and valvular heart disease. Patients with chronic or paroxysmal atrial fibrillation can present with AMI due to coronary embolism, and thrombus aspiration with percutaneous aspiration device is a feasible treatment option for these patients along with anticoagulation the rapy.

3D 광학 스캐너를 이용한 인체모형 팬텀 표면 데이터 획득 및 X-ray 에너지의 몬테카를로 시뮬레이션(MCs)을 이용한 팬텀 선량 평가와 영상구현

신준봉(Jun-Bong Shin),이도희(Do-Heui Lee),윤경준(Kyoung-Jun Yoon),박민재(Min-Jae Park),고영문(Young-Moon Go),김성우(Sung-Woo Kim),홍승모(Seung-Mo Hong),이현철(Hyun-Cheol Lee),이의섭(Ui-Seob Lee),노선영(Seon-Yeong No),강석윤(Seok-Yoon 대한영상의학기술학회 2021 대한영상의학기술학회 논문지 Vol.2021 No.-

목 적:본 연구의 목적은 기존 컴퓨터단층촬영(Computed tomography, 이하 CT) 스캔 방법 대신 3D 광학 스캐너 (3D optical scanner)를 이용하여 인체모형 팬텀의 표면 데이터를 획득하고 기존 CT 스캔 표면 데이터와 3D 광학 스캔 표면 데이터 차이를 비교 분석하는 것이며, 3D 광학 스캔 표면 데이터를 몬테카를로 시뮬레이션(Monte Carlo Simulation, MCs)에 적용하여 일반 촬영 에너지조건으로 팬텀 선량 평가와 영상 구현을 연구하는 것입니다. 연구방법: 일반촬영의 선량 평가를 위해 환자 표면 데이터를 얻을 때 3D 광학 스캐너를 활용할 것을 제안합니다. 우리는 3D 광학 스캐너(Go!SCAN SPARKTM, CREAFORM) 사용하여 기존 CT 스캔 방법을 대신해 인체모형 팬텀의 표면 데이터를 얻었습니다. 3D 스캔 표면 데이터를 정렬하고 편집하기 위해 Meshlab 프로그램을 이용하였으며, 3D slicer 프로그램을 이용하여 CT 스캔 표면 데이터와 3D 스캔 표면 데이터 차이를 정량적으로 비교분석 하였습니다. 또한 3D 스캔 표면 데이터 분석 프로그램(VXelements, CREAFORM)을 사용하여 인체 모형 팬텀의 재현성을 평가 하였습니다. SpekCalc 프로그램을 이용하여 X-ray beam 에너지 스펙트럼을 계산하고 Geant4 기반의 MCs인 TOPAS를 이용하여 X-ray Beam을 모델링 하였습니다. 인체모형 팬텀을 MCs에 적용 하여 일반촬영 에너지 조건으로 팬텀 선량을 평가하고 Phase space data 통해 영상을 구현을 연구 하였습니다. 결 과:3D slicer를 이용한 CT 스캔 표면 데이터와 3D 광학 스캔 표면 데이터의 정량적 비교 분석에서 Hounsdroff distance는 Average 2.45 mm, Dice coefficient는 0.97로 나타나 CT 스캔 표면 데이터와 3D 스캔 표면 데이터가 거의 일치하는 것을 확인할 수 있었습니다. 또한 인체모형 팬텀 재현성 평가에서 인체모형 팬텀의 AP position, RAO position, LAO position 기준으로 팔이 있는 팬텀의 error distribution은 2 mm 오차 범위에서 평균 89.78%가, 4 mm 오차 범위에서 96.05%가 일치하는 것으로 나타났으며, 팔을 제외한 경우 error distribution은 2 mm 오차 범위에서 평균 96.48%가, 4 mm 오차 범위에서 99.45%가 일치하는 것으로 나타나 3D 광학 스캐너의 표면 데이터 재현성이 우수한 것으로 나타났습니다. 인체모형 팬텀을 이용한 3D 스캔 표면 데이터를 MCs에 적용하여 전신일반촬영 에너지 조건으로 팬텀선량 평가가 가능하며, Phase space data 통해 영상을 구현할 수 있다는 것을 확인 하였습니다. 결 론:본 연구에서 3D 광학 스캐너를 사용한 인체모형 팬텀 데이터 획득은 기존 CT scan 방식보다 간편하고 방사선 노출 없이 다양한 위치와 균일한 표면 구조의 정보를 정밀하게 얻을 수 있으며 MCs을 이용한 선량계산이 가능하며, Phase space data 통해 영상을 구현이 가능함에 따라 앞으로 일반촬영 분야의 활용과 선량평가에 매우 유용할 것으로 생각 됩니다. Purpose:The purpose of this work is to acquire surface data of anthropromophic phantom using 3D optical scanner instead of conventional computed tomography (CT) scanning methods and to compare the differences between existing CT scan surface data and 3D optical scan surface data. also it is to apply 3D optical scan surface data to Monte Carlo simulations (MCs) to study whether phantom dose evaluation and image implementation are possible under general shooting energy conditions. Targets and Methods:We suggest utilizing a 3D optical scanner when obtaining patient surface data for dose evaluation of normal photography. We obtained surface data of anthropromophic phantom instead of conventional CT scanning methods using 3D optical scanners (Go!SCAN SPARKTM, CREAFORM). The Meshlab program was used to sort and edit 3D scan surface data, and the CT scan surface data and 3D scan surface data differences were quantitatively compared using the 3D slicer program. The reproducibility of the dummy phantom was also evaluated using the 3D Scan Surface Data Analysis Program (VXelements, CREAFORM). Using the SpekCalc program, the X-ray beam energy spectrum was calculated and the X-ray beam was modeled using TOPAS, a Geant4-based MCs. We applied the anthropromophic phantom to MCs evaluate the phantom dose in X-ray energy conditions and study whether the image can be implemented through phase space data. Result:Quantitative comparative analysis of CT scan surface data and 3D optical scan surface data using 3D slicer showed that Hounsdroff distance was Average 2.45 mm and Dice coefficient was 0.97, indicating a close match between CT scan surface data and 3D scan surface data. In addition, the anthropromophic phantom reproducibility assessment shows that the error distribution of the phantom with arms by AP position, RAO position, and LAO position of the anthropromophic phantom averaged 89.78% in the 2 mm error range and 96.05% in the 4 mm error range. MCs using anthropromophic phantom confirmed that 3D scan surface data can be applied to Monte Carlo simulation to evaluate phantom dose under X-ray energy conditions through phase space data. Conclusion:In this study, anthropromophic phan tom data acquisition using 3D optical scanners is simpler than conventional CT scan methods, provides precise information of various locations and uniform surface structures without radiation exposure, enables dosing using MCs, and enables image implementation through phase space data.

Offset voltage injection method for neutral‑point AC voltage ripple suppression in Vienna rectifiers

Young-Min Go,June-Seok Lee 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9

This paper proposes an offset voltage injection method to compensate for AC voltage ripple at the neutral-point of a Vienna rectifier. The proposed method leads to sinusoidal input currents and has a suppression effect on the AC voltage ripple at the neutral-point by injecting three offset voltages to remove both the zero current distortion and voltage unbalance (both DC voltage and AC voltage ripple) at the neutral-point. This can result in an improvement in the Total Harmonic Distortion (THD) of the input current. The offset voltage to realize the suppressing of the AC voltage ripple is defined based on a neutral-point voltage equivalence model of a Vienna rectifier. The priority of the offset voltages is considered to avoid over-modulation. In addition, incompatibility between offset voltages is analyzed to guarantee sinusoidal input currents. By considering both of these characteristics, the proposed method injects the offset voltages in order from the higher priority to the lower priority. The performance and effectiveness of the proposed method are verified with simulation and experimental results.

Association of an Anti-inflammatory Cytokine Gene IL4 Polymorphism with the Risk of Type 2 Diabetes Mellitus in Korean Populations

Go, Min-Jin,Min, Hae-Sook,Lee, Jong-Young,Kim, Sung-Soo,Kim, Yeon-Jung Korea Genome Organization 2011 Genomics & informatics Vol.9 No.3

Chronic inflammation has been implicated as one of the important etiological factors in insulin resistance and type 2 diabetes mellitus (T2DM). To investigate the role of anti-inflammatory cytokines in the development of T2DM, we conducted a case-control study to assess the association between IL4/IL4R polymorphisms and disease risk. We firstly identified single nucleotide poly-morphisms (SNP) at IL4 and IL4RA loci by sequencing the loci in Korean participants. Case-control studies were conducted by genotyping the SNPs in 474 T2DM cases and 470 non-diabetic controls recruited from community-based cohorts. Replication of the associated signals was performed in 1,216 cases and 1,352 controls. We assessed effect of IL4 -IL4RA interaction on T2DM using logistic regression method. The functional relevance of the SNP associated with disease risk was determined using a reporter expression assay. We identified a strong association between the IL4 promoter variant rs2243250 and T2DM risk (OR=0.77; 95% CI, 0.67~0.88; p=$1.65{\times}10^{-4}$ in the meta-analysis). The reporter gene expression assay demonstrated that the presence of rs2243250 might affect the gene expression level with ~1.5-fold allele difference. Our findings contribute to the identification of IL4 as a T2D susceptibility locus, further supporting the role of anti-inflammatory cytokines in T2DM disease development.

Poster Session : The Effect of Sling Exercise on Muscles` Thickness, Function, and ROM in Rotator Cuff Surgery Patients

( Min Young Wang ),( Min Sul Myung ),( Yun Hee Lee ),( Young Ah Park ),( Seon Woong Jang ),( Go Eun Hong ),( Seok Hwan Kim ),( Dong Hee Kim ),( Ha Yan Lee ) 한국체육학회 2013 In Pursuit of Evidence-Based Sport and Exercise Sc Vol.0 No.-

Effect of Genetic Predisposition on Blood Lipid Traits Using Cumulative Risk Assessment in the Korean Population

Go, Min-Jin,Hwang, Joo-Yeon,Kim, Dong-Joon,Lee, Hye-Ja,Jang, Han-Byul,Park, Kyung-Hee,Song, Ji-Hyun,Lee, Jong-Young Korea Genome Organization 2012 Genomics & informatics Vol.10 No.2

Dyslipidemia, mainly characterized by high triglyceride (TG) and low high-density lipoprotein cholesterol (HDL-C) levels, is an important etiological factor in the development of cardiovascular disease (CVD). Considering the relationship between childhood obesity and CVD risk, it would be worthwhile to evaluate whether previously identified lipid-related variants in adult subjects are associated with lipid variations in a childhood obesity study (n = 482). In an association analysis for 16 genome-wide association study (GWAS)-based candidate loci, we confirmed significant associations of a genetic predisposition to lipoprotein concentrations in a childhood obesity study. Having two loci (rs10503669 at LPL and rs16940212 at LIPC) that showed the strongest association with blood levels of TG and HDL-C, we calculated a genetic risk score (GRS), representing the sum of the risk alleles. It has been observed that increasing GRS is significantly associated with decreased HDL-C (effect size, $-1.13{\pm}0.07$) compared to single nucleotide polymorphism combinations without two risk variants. In addition, a positive correlation was observed between allelic dosage score and risk allele (rs10503669 at LPL) on high TG levels (effect size, $10.89{\pm}0.84$). These two loci yielded consistent associations in our previous meta-analysis. Taken together, our findings demonstrate that the genetic architecture of circulating lipid levels (TG and HDL-C) overlap to a large extent in childhood as well as in adulthood. Post-GWAS functional characterization of these variants is further required to elucidate their pathophysiological roles and biological mechanisms.