노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

발생원 유형에 따른 공기 중 휘발성 유기화합물의 비산배출 특성 평가

백성옥,김미현,서영교 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.4

In this study, the fugitive emission characteristic^ of airborne volatile organic compounds from different source categories were evaluated with respect to the concentrations measured in the vicinity of the sources. A total of 22 different sources were investigated, including gasoline storage and filling stations, painting spray booth, laundry, printing offices, textile industries, and a number of environment sanitary facilities such as landfill, wastewater treatment and incineration plants The target VOCs included 83 individual compounds, which were determined by adsorption sampling and thermal desorption coupled with GC/MS analysis. Overall, the aliphatic compounds appeared to be the most abundant class of compounds in terms of their concentrations, followed by aromatic, and halogenated hydrocarbons. As a single compound, however, toluene was the most abundant one, explaining 11% of the total VOC concentrations as an average of all the dataset. Among source categories. petroleum associated sources such as gasoline storage/filling stations, and laundry factory were identified as the most significant sources of aliphatic hydrocarbons, while aromatic VOCs were dominantly emitted from the sources handling organic solvents, such as painting booth, printing offices, and textile dyeing processes. However, there was no apparent pattern in terms of the contributions of each group to the total VOCs concentrations in environmental sanitary facilities. It was also found that the activated carbon adsorption tower installed for the VOC emission control in some facilities do not show any effective performances, which may result in the increased VOC levels in the ambient atmosphere.

후직장의 위장관 간질 종양 1예

최영일,이승현,안병권,백승언 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.2

The retrorectal gastrointestinal stromal tumor is very rare. An 24-year-old woman patient was admitted for huge pelvic mass with constipation, lower abdominal discomfort. The pelvic mass was palpable on posterior rectal wall on digital rectal examination. On exploration, tumor excision was performed. Gross finding shows 14x11x10.5 cm sized grayish tan and irregular mass. The cut surface is solid, bright yellow to grayish yellow with foci of hemorrhage and calcification. The tumor show spindle shaped cells and mitosis. It showed positive staining for c-kit. The tumor was diagnosed as gastrointestinal stromal tumor in rectrorectal space.

구강 및 악안면 영역의 감염증에 관한 임상적 연구

백진현,이영수,심광섭 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.2

This is a retrospective study on the patients with infection of the oral and maxillofacial region with the purpose of obtaining some useful data for diagnosis and treatment plan of that relatively common disease in dentistry. The used materials of study were 87 in total, including 52 male patients, 35 female patients who diagnosed and treated at the Department of the Dentistry in Hanyang Medical College Hospital for the period of Jan. 1990 to Dec. 1994. The author analyzed the distribution and incidence of sex, age, admission period, etiologic factors, etiologic teeth, treatment method of infections, pus culture, antibiotics sensibilities and medication. The result obtained as follows : 1. The developmental incidences by sex was superior in male by the ratio of 1.5 : 1 and the infection was most frequently occurred during the third decades(35.6%). 2. The number of admitted patients elevated in February, March, and April, and average of admission period was 9.8 days. 3. Main etiologic teeth showed on lower molar region in adult(63%) and upper molar region in primary dentition(46.1%). 4. Medications were administrated in all of the cases, and surgical incision and drainage were performed in 53% and extraction of the causative teeth were performed in 63.6% of all cases. 5. The most common involved fascial spaces were Buccal space(41.4%), Infraorbital space(27.6%), Submandibular space(16.1%),in order, and 9 cases(10.3%) were Ludwig's Angina. In 68.2% of the patients, and infection involved only one fascial space and in 21.8% of the patients, it involved to more fascial spaces. 6. The most causative organisms isolated from pus culture were Gram-positive facultative cocci(55.5%), and antibiotics sensitivities on the total isolated bacterial strains were exposed chloramphenicol(88.6%), Cephalothin(88.6%), Erythromycin(81.5%), Lincomycin(77.8%) in order, but it showed resistant on Gentamycin(58.3%), Tetracycline(56.5%), Methicillin(38.5%).

一部 男子大學生들과 運動選手들의 一側優位性(左右差:手·足·體·眼)에 關한 比較硏究

백현중,윤태영,최중명,박순영 慶熙大學校 1991 論文集 Vol.20 No.-

This study is from March 1st, of 1988 to September 31, 1988. The subjects of study consists of students in general who are in attendance at university and all samples are selected at random. The total number of subjects counts to 187(sports man : 56, College students : 131) and the researcher measures and analyses the subjects' physique, physical strength, the test of dominance eye, the functional tests of hand, foot, body and eye. The results as follows : 1. Physical growth and development Mean values of body height are 177.19±5.01㎝ for sports man and 173.3±0.46㎝ for college student and superior to standard value of Korean. Mean values of body weight are 69.6.±6.17㎏ for sports man and 66.1±1.68㎏ for college student, and the mean values of chest girth are 91.86±4.83㎝ and 94.1±1.53㎝. Mean values of sitting height are 93.0±4.28㎝ and 93.3±1.05㎝. 2. Physical fitness Mean values of grin strength are 41.6±4.3 for right side and 44.0±6.0 for left side in sports man and 44.2±1.4 for right and 46.3±1.7 for left in college student. Mean values of arm strength are 29.7±3.9 for right and 31.8±2.9 for left in sports man 25.±1.4 or right and 28.7±1.9 for left in college student. Mean values of leg strength are 38.4±5.8 for right and 43.8±6.1 for left in sports man and 43.5±1.8 for right and 44.2±1.1 for left in college student. 3. Functional rate of foot, trunk, eye and hand As considered according to its functions, the rates of right foot, left foot, both of feet in both of the sports man and college student, total rates are each of 83.3%, 16.7%, 2.4% for sports man and 53.7%, 39.0%, 2.6% for college student. The rates of right side, left side, both of sides in both of the sports man and college student, the total rates are each of 53.3%, 37.4%, 6.7% for sports man and 39.1%, 46.1%, 5.1% for college student. The rates of right eye, left eye, both of eyes in both of the sports man and college student, the total rates are each of 53.6%, 31.7%, 4.1% for sports man and 53.3%, 20.8%, 22.7% for college student. The rates of right hand, left hand, both of hands in both of the sports man and college student, the total rates are each of 67.5%, 21.8%, 5.2% for sports man and 84.5%, 10.4%, 2.2% for college student, respectively. 4. Tests of dominance eye As considered according to tests of dominance eye, the results of right eye, left eye in both sports man and college student, results are each of 62.5%, 37.5% for sports man and 70.2%, 29.8% for college students, 67.9%, 32.1% for the total of sports man and college student, respectively.

세침 흡인술을 이용한 종창된 림프절의 세포학적 평가

백강현,윤기영,최호정,정성목,이영원,조성환,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

Fine needle aspiration(FNA) cytology is considered as the diagnostic technique of choice in the assessment of swellen lymph nodes. The aim of this study is to determine the indication and diagnostic method of FNA of lymph node lesions performed at Veterinary Medicine Teaching Hospital of Chungnam National University. The records of 16 patients which had undergone FNA during the study period were retrieved. The cytological results were classified as lymphadenitis, lymphoid hyperplasia and lymphoma. Lymphoma cases were classified as B-cell origin and T-cell by immunophenotyping. The result of the FNA cytological diagnosis showed that nine (56%) of the patients were lymphoma, five (31%) of the patients were lymphoid hyperplasia and two (13%) of the patients were lymphadenitis.

연준모치, Phoxinus phoxinus와 금강모치, Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택

백현민,송호복,심하식,김영건,권오길 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

강원도 정선군의 동대천에 동서하고 있는 Phoxinus phoxinus와 Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택에 대하여 2001년 4월부터 9월까지 계절별로 조사, 연구하였다. 두 종은 전형적인 Aa형 하천에 서식하고 있었으며, 상류쪽에서는 P. phoxinus가, 중·하류쪽에서는 R. kumgangensis가 우세하였고, 수온이 중요한 서식 제한 요인으로 나타났다. 동일 서식지에서 P. phoxinus는 유속이 느린 곳에, R. kumgangensis는 상대적으로 유속이 빠른 곳에서 점유율이 높게 나타났으며, P. phoxinus는 하천의 하층에, R. kumgangensis는 상층 및 중층에 주로 서식하였다. 먹이 생물은 P. phoxinus는 봄에 Ephemeroptera, Trichoptera, Diptera를 주로 섭식하였고 여름과 가을에는 Diptera를 섭식하였으며, R. kumgangensis는 봄과 여름에는 육상곤충, 가을에는 Diptera를 주로 섭식하였다. The authors investgated habitat segregation and prey selectivity of two cohabitants, Phoxinus phoxinus and Rhynchocypris kumgangensis, in Dongdeacheom, Jeongsoen-gun, Kangwon-do, Korea, from April to October 2001. P. phoxinus dominated the upper reaches of the stream, whereas R. kumgangensis dominated the middle and lower reaches of the upprer stream. P. phoxinus inhabited zones of relatively slow water velocity in the bottom layer, but R. kumgangensis lived zoned of relatively high water velocity in the middle and upper layers of the cohabitat. P. phoxinus ingested mainly Ephemeroptera, Trichoptera, Diptera in spring, and Diptera in summer and autumn. R. kumgangensis fed mainly on terrestrial insects in spring and summer, and Diptera in autumn.

연준모치,Phoxinus phoxinus 와 금강모치, Rhynchocypris kumgangensis의 서식지 분리와 먹이 선택

백현민,송호복,심하식,김영건,권오길 江原大學校 附設 環境硏究所 2002 環境硏究 Vol.19 No.-

The authors investigated habitat segregation and prey selectivity of two cohabitants, Phoxinus phoxinus and Rhynchocypris kumgangensis, in Dongdaesheon, Jeongsoen-gun, Kangwon-do, Korea, from April to October 2001. P. phoxinus dominated the upper reaches of the stream, whereas R. kumgangensis dominated the middle and lower reaches of the upprer stream. P.phoxinus inhabited zones of relatively slow water velocity in the bottoom layer, but R. kumgangensis lived zones of relatively high water velocity in the middle and upper layers of the cohabitat. P. phoxinus ingested mainly Ephemeroptera, Trichophtera, Diptera in spring, and Diptera in summer and autumn. R. kumgangensis fed mainly on terrestrial insects in spring and summer, and Diptera in autumn.

全蝎 抽出物이 血栓症, 全腦虛血 및 腦細胞毒性에 미치는 影響

백명현,황영근,정지천,강정준,김성훈 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 전갈 추출물이 어혈병태 모델과 KCN으로 유발된 전뇌허혈, 뇌세포독성 등에 미치는 영향을 관찰하였다. 전갈은 Dextran에 의해 유도된 혈전으로 감소된 혈소판수, fibrinogen량, hemocrit치를 유의성 있게 증가시키고. 증가된 prothrombin time을 유의성 있게 단축시켰다. 전갈은 thrombin과 ADP에 의해 유도된 혈소판 응집을 억제하였으나 collagen에 의해 유도된 혈소판 응집에는 저해효과를 나타내지 않았다. 또한 collagen과 epinephrine에 의해 유도된 pulmonary embolism에 대하여 보호 효과를 나타내었다. 전갈은 KCN에 의한 전뇌허혈 유발 실험에서 혼수시간을 유의성 있게 단축시켰고, Amyloid β protein(25-35)에 의해 유도된 PC12 세포의 독성에 대하여 보호효과를 나타내었다. This following is effect of Buthus martensi Karsch (BMK) extract on dextran-thrombus model, KCN-induced coma, cytotoxicity of brain etc. BMK extract significantly increased number of platelet and fibrogen and significantly shortened the prothrombin time as compared with control group treated with dextran. BMK extract didn't affect the changes of hematocrit as compared with control group treated with dextran. BMK extract induced a significant inhibition of human platelet aggregation induced by thrombin and ADP but did not affect human platelet aggregation induced by collagen. BMK extract showed a protective effect on pulmonary thrombosis induced by collagen and epinephrine. BMK extract prolonged the duration of KCN-induced coma and showed a protective effect on cytotoxicity of PC12 cells induced by amyloid β protein(25-35) in a dose dependent manner. These results suggested that BMK extract might be usefully applied for prevention and treatement of thrombosis and brain damage.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.