2차원적 전기영동에 의한 톡소포자층 당단백 항원의 분석

황일영,최인욱,신대환,이영하 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Serious disease due to Toxoplasma gondii occurs in congenitally infected children and in immunosuppressed patients, particularly in the case of HIV infection. Glycoproteins modulate the physicochemical properties, and are involved in biological and immunological activities. Although there are many reports about the immune responses after T. gondii infection, there are few reports to identify the glycoproteins from T. gondii. For this reason, we purified the glycoproteins from tachyzoites and cysts of T. gondi and then evaluated the antigenic characterizations of toxoplasmic glycoproteins. Tachyzoites of T. gonii were purified by Percoll-gradient centrifugation. Glucosamine was the major monosaccharides released from the tachyzoites of T. gondii by acid hydrolysis methods. In order to define the antigenicities of both strains, we did 2-dimensional electrophoresis(2-DE). Over 40 protein spots were reproducibly separated in tachyzoites of T. gondii by 2-DE in range of the pH 5-8. Glycoproteins were isolated from tachyzoites of T gondii by Con A-Sepharose 4B affinity chromatography. The major bands of glycoproteins were approximately 22 kDa, 30 kDa and 38 kDa in tachyzoites of T. gondii.

고온에서 회전하는 터빈엔진 디스크의 응력해석

황수철,국정한,조재웅,하영민 한국공작기계학회 1995 한국생산제조학회지 Vol.4 No.4

This study includes thermal plasticity analyses for a turbine rotor with the simple geometry and the boundary conditions. When centrifugal or thermal stress are applied at the high temperarture material of engine blade, stress distributions in material σ_rr, σ_θθ, τ_rθ. Mises stress) are analyzed by computer simulation(ABAQUS) as followings; 1. The maximum stress at the radial direction (σ_rr) is applied at the upper middle part of spline hole. 2. The maximum stress at the tangential direction (σ_θθ) is applied at the upper right boundary of spline hole. 3. The maximum shear stress (τrθ) in (-) direction is applied at the upper middle part of spline hole. 4. The maximum Mises stress is applied at the upper right boundary of spline hole. This stress is due to the critical stress by which rotor can be fractured according to elapsed time.

디메틸 실록산과 2-히드록시에틸메타크릴레이트계 블락공중합체의 합성 : 초임계 이산화탄소용 양손잡이형 계면활성제 Ambidextrous Surfactant for Supercritical Carbon Dioxide

황하수,이민영,임권택 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

Block copolymers containing dimethy siloxane and 2-hydroxyethyl methacrylate sequences were synthesized by group transfer polymerization (GTP) of 2- trimethylsilyloxy ethyl methacrylate(TMS-EMA) using silyl ketene acetal terminated poly(dimethylsiloxane) (PDMS) as macroinitiator, followed by hydrolysis of TMS-EMA to HEMA. The block copolymers were obtained with controlled molecular weight and narrow molecular weight distribution. Trimethylsilyl groups in the P(TMS-EMA) block could be selectively hydrolyzed without interfering with Si-O bond in PDMS block. The block copolymers formed micelles in methanol, the effective diameters (Rh) of which were in the range of 78 ~110 nm with narrow distribution by dynamic light scattering (DLS). The latex particles, PMMA was synthesized by dispersion polymerization in supercritical carbon dioxide with these block copolymers as stabilizer.

强風의 發生成因別 時間的 變化에 관한 硏究

황진득,하영철,곽윤근 金烏工科大學校 1994 論文集 Vol.15 No.-

The wind resistant design of building generally used up to data has been based on the value of an extreme speed. The extreme speed is normally estimated from wind data observed for the return periods of last 50 years or 100 years on the basis of the importance of building to be constructed. However, it is essential to consider the time variance of wind action because strong winds originated by Typhoon or others are occationally coming for attack. This study is to examine the origin of occurence and the action time of strong wind, and to understand the time variance characteristics. Strong winds are divided into 3 parts such as the regional condition of Korea, namely Typhoon, Frontal system storm, and Monsoon. The areas reflecting the origin of 3 kinds of the strong winds are also devided are selected as such as Seoul-Kyongki, Jungbu, and South-seashore. The representative cities are selected as Seoul fro, Uljin and Kunsan for, and Busan and Yeosu for. Observed wind data of past 20 years will be employed to estimate duration and propability of wind velocity. The observed wind data are recorded with duration of 96 hours at the center that annual extreme wind speed takes place, and with the mean value of wind velocity during 10 minutes at 1 hour interval. Based on these investigations, the duration and probability of wind velocity should be estimated. Generalized probability distribution function of wind velocity will be proposed. Duration of strong winds presented as a function of its maximum wind velocity. From these results, multipling generalized distribution function by generalized number of (wind velocity) samples, approximate equation of wind-duration will be derived. Approximate equation of wind-duration proposed in this study should be useful for analysing fatigue damage of structures and time dependent analysis of structure under strong wind in Korea.

인버터 制御車輛 電源用의 컨버터 制御特性

황준하,서영수,임영배 明知大學校 産業技術硏究所 1994 産業技術硏究所論文集 Vol.13 No.-

In this paper,the inverter-voltage source of AC Electrical Vehicle System has generally used voltage type PWM converter. A parallel operation of two single-phase voltage typed PWM converters used IGBT which achives the bidirectional power floe between a single phase AC supply and a DC bus voltage is described. The parallel operating system has reduced current ripple of a source than a single device operation, and made a converter capacity increased.Also,the voltage type PWM converter made the input current wave as sinusoidal wave, and performs the high power factor driving.

신기능성 물질 탐색을 위한 침엽수잎의 특수성분 분석에 관한 연구 (Ⅰ) : 펙틴, 탄닌, 테르페노이드의 분석 Analysis of Pectin, Tannin and Terpenoids

황병호,이현종,강하영,유순희,조재현,조국란 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

요 약 주요 소나무류잎의 특수성분을 분석하여 시판되고 있는 솔잎 가공식품의 기초데이터를 제공함과 동시에 임산화학 분야의 학문적 기초데이터를 축적하기 위하여 실시한 결과, 펙틴은 잣나무가 가장 높게 분석되었으며, 탄닌은 잣나무가 높게 분석되었고, 소나무와 해송은 유사하였다. 테르페노이드 성분중 α-pinene은 소나무와 잣나무에서, β-pinene은 리기다와 해송에서 높게 분석되었으며, 희귀성분으로는 잣나무에서 sabinene과 citronellol, 리기다에서는 α-pinene oxide 등이 분석되었다. 특히 소나무일체 다량 존재하는 성분은 α-pinene, β-caryophyllene, β-phellandrene, Δ3-carene, phytol 등 이었다. 향기성분의 주체인 α-pinene, limonene, bornylacetate들이 소나무와 잣나무에서 보다 많이 분석된 것으로 보아 삼림욕의 상쾌한 내용의 주체성분이라고 생각된다. ABSTRACT Pectin and tannin analysis were carried out to inverstigate any available components from Pinus densiflora, koraiensis, P. thunbergii and P. rigida. To analyze terpenoid components, the essential oils were extracted with steam distillation method from four kinds of pine needles. The essential oil was analyzed by GC and GC-MS spectroscopy.The results were summarized as follows: Pectin content was highest in P. koraiensis with 0.40%, and tannin content was highest in P koraiensis with 1.05. Major components of P. densiflora needles were α-pinene, β-caryophyllene, Δ3-carene and phytol. α-Pinene, Δ3-carene, β-caryophyllene, germacrene D, and camphene were found major components n P. koraiensis. Major components of P. thunbergii needles were β-pinene, α-pinene, β-caryophyllene and germacrene D. β-Pinene, α-pinene, humulene oxide and α-elemene were major components in P. rigida. Sabinene and citronellol were infrequent components in P. Koraiensis, and α-pinene oxide was present only in P. rigida. α-Pinene, limonene, and bornylacetate well known as the mail components of green air bath were found in P. densiflora and P. koraiensis.

에탄올-물 혼합용매내에서 2,4,6,N-Tetramethyl Pyridinium Iodide의 회합에 대한 압력효과

黃正儀,池鍾基,李永華,禹義夏 慶北大學校 物理化學硏究所 1984 硏究論文集 Vol.5 No.-

부피 퍼센트 95%의 에탄올-물 혼합용매내에서 2,4,6,N-테트라메틸 피리디늄요오드(TeMPI)의 이온회합 상수(K)를 수정한 자외선분광 및 전기 전도도 혼용법으로 온도범위 25∼50℃, 압력범위 1∼2,000bars에서 결정하였다. K값은 압력이 증가함에 따라서 증가하였고 40℃에서 최대값을 나타내었다. 부분몰부피변화(ΔV)는 비교적 작은 음의 값이 었으며 ΔV의 절대값은 40℃에서 최소값을 보여주었다. TeMPI의 이온 크기 변수(a)는 40℃에서 최대값을 가졌다. ΔH˚값은 40, 25℃ 그리고 50℃에서 각각 영, 음수 및 양수로 나타났으며 엔트로피(ΔS˚)와 자유에너지(ΔG˚)와 같은 다른 열역학변수 값도 계산하였다. 이와 같은 실험결과로부터 우리들은 TeMPI가 압력증가에 대하여 안정화되고, 40℃까지는 온도증가에 따라서도 안정화됨을 보여주었다. 그러나 40℃에서는 TeMPI 두 분자에 있는 8개 메틸기의 분자간 소수성 상호작용으로 인하여 약한 이량체를 형성하고 50℃ 이상에서는 다시 열적으로 분해된다는 결론을 얻게 되었다. The ionic association constants(K) of 2,4,6,N-tetramethyl pyridinium iodide(TeMPI) in 95 volume percentage ethanol-water mixture were determined by a modified UV and conductance method at 25℃ to 50℃ under 1 to 2,000 bars. The K values increase with increasing pressure and have maximum value at 40℃. The partial molar volume change (ΔV) has relatively small negative value and the absolute values of Δ are minimum at 40℃. The ion size parameter(a) of TeMPI have maximum value at 40℃. ΔH˚ values are zero, positive and negative at 40℃, 25℃ and 50℃ respectively. Other thermodynamic parameters such as the changes of standard entropy (ΔS˚) and free energy(ΔG˚) were evaluated. From these experimental results, we came to conclusion that TeMPI is stabilized by the elevation of pressure and that of temperature below 40℃ but weakly dimerized at 40℃ because of the intermolecular hydrophobic interaction of eight methyl groups of two molecules. And it thermally decomposed above 50℃.

C_3 및 C_7위치에 치환된 새로운 세파로스포린 化合物의 合成과 抗菌活性(Ⅰ)

황화영,하재천,김영수,고옥현,강형룡 조선대학교 약학연구소 1994 藥學硏究誌 Vol.16 No.1

The synthesis and antimicrobial activity of cephalosporin having (Z)-2-(2-aminothiazol-4-yl)-2-trityloxyiminoacetamido group and 5-(2-chlorophenyl)-4-phenyl-4H-1, 2, 4-triazol-3-thiomethyl group on the C_3 and C_7 position of the cephem ring, respectively are descrived. This compound was tested for antimicrobial activity in vitro against Candida albicans ATCC 10231, Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 6538P, Mycobacterium phlei IIP-IPH, Escherichia coli KCTC 1039, Escherichia coli ESS, Klebisiella pneumoniae KCTC 1560, Salmonella typhymurium TV-119, Pseudomonas aeruginosa IFO 13130. The antimicrobial activity of synthetic compound was better as compare with cefotaxime and cefazoline against Micrococcus luteus ATCC 9341 & Escherichia coli KCTC ESS.

工業專門大學 熱冷凍管理科 敎育課程 開發에 關한 硏究

金夏永,張光鎭,黃龍夏 忠州大學校 1982 한국교통대학교 논문집 Vol.15 No.2

A field of Heat and Refrigeration are radical growing in modern industry socity. So, we developed the department of Hear and Refrigeration curriculum that will be in charge of this field. The present study has contents that will be able to educate about the field of Boiler and Heat, Refrigeration and Air conditioning. In order to educate semi-engineer in this field, this curriculum was established by the subject analysed each job group. Also, we stuied this curriculum through the reference of in and outside the country, consultation of the experts of industry and college. We opened the seminar about this study. In the present study, we developed A curriculum on the department of Heat and Refrigeration and Course of Heat and Refrigeration. By refer to upward reference consultation and Seminar.

6 시그마 기법을 이용한 임상약동학 자문 업무의 효율성 개선 : 신속성과 정확성을 중심으로 Focusing on Speed and Accuracy of CPS

김민영,박효정,박향미,손기호,최경업,박영하 한국병원약사회 2001 병원약사회지 Vol.18 No.2

연구 배경 : 적정한 약물요법을 순발력 있게 시행하기 위해서는 CPS 업무가 신속, 정확하게 수행되는 것이 필수적임에도 불구하고 지금까지 CPS 업무의 신속성, 정확성을 향상시키기 위한 객관적인 업무 표준은 물론 그의 측정까지도 전무한 실정이었다. 이에 수요자인 의사의 측면에서, CPS 자문결과의 신임도에 영향을 미치는 업무의 순발력과 정확성을 향상시키기 위해 CPS 업무의 신속성, 정확성에 영향을 미치는 요인을 파악, 분석하고 이를 향상시킬 수 있는 업무 표준을 개발하는 것을 목표로 6-Sigma 기법을 사용하여 본 연구를 진행하였다. 방 법 : CPS 업무의 신속성 평가를 위해 전체 업무 과정과 부분별 업무 과정에 대한 소요 시간을 측정하였고, 정확한 평가를 위해 예측되는 Cpeak와 실제 측정된 Cpeak를 비교하였다. CPS 업무에 대해 6-Sigma 기법을 사용하여 개선이 필요한 사항을 파악하고 이에 대한 개선활동을 한 후, 개선 활동 전과 후의 CPS 업무의 신속성과 정확성의 σ 수준을 각각 측정하여 이의 향상 정도를 잠재적인 경제적 이익으로 환산하였다. 결 과 : 신속성은 제 1차 자료수집 기간(대조군, 현황 파악 기간) 중 총 소요시간은 평균 930.6±583.2이었으며, 채혈-검체 접수 : 140.4±8705분, 검체접수-약물농도 결과보고: 48.4±23.1분, 결과보고-CPS 보고서 작성 : 741.8±510.6분으로 측정되었다. σ 수준은 총 소요시간의 경우는 0.56σ, 단계별로는 각각 0.45σ, 5.70σ, 0.30σ이었다. 제 2차 자료수집 기간(개선군, 개선활동 평가 기간) 중 총 소요시간은 평균 659.1±344.1분이었고 단계별로는 각각 92±66.2분, 48±28.0분, 518.9±340.7분이 소요되었다. σ 수준은 총 소요시간은 1.75σ이고, 단계별로는 1.32σ, 4.70 σ, 1.10σ로서 총 소요시간과 단계별 소요시간이 1차 자료수집기간에 비해 향상되었다. 정확성 측면에서는, 대조군의 경우, 예측 Cpeak와 실측 Cpeak의 차이는 평균은 0%, 분산 ±27.10%, σ 수준은 -0.56σ이었고 개선군의 평균과 분산 그리고 σ 수준은 각각 0%, ±15.10%, 0.14σ로 향상되었다. CPS 신속성, 정확성 향상으로 인한 경제적 이익은 정확성 측면에서 380만원/년, 신속성 측면에서 1350만원/년으로 총 1730만원/년의 비용절감 효과가 있었다. 결 론 : CPS 업무 흐름에 따라 CPS 효율성에 영향을 미치는 요인을 분석한 결과 CPS 효율성 향상을 위해서는 투약관련 의무 기록의 정확성, 임상병리 검사의 정도관리 향상, 그리고 관련 부서와의 원활한 업무 협조가 가장 중요하였다. The effectiveness of CPS (Clinical Pharmacokinetics Service), one of pharmacist's clinical services, was emphasized by many studies since 1985 it was introduced Korea about. But in the view of clinicians (medical doctors), customers, it's convenience or reliability was not properly evaluated for applying it to patient's care. To improve quality of Clinical Pharmacokinetics Service (CPS), the speed and the accuracy of CPS, the key points of efficiency in CPS, were evaluated by using 6-Sigma method. The speed of CPS was evaluated using total hours it took from blood sampling by a nurse to sending a CPS report to the patient's chart by a clinical pharmacist. Total hours were divided into blood sampling-to-sampling registering, sampling registering-to-C_(peak) result reporting, and C_(peak) result reporting-to-pharmacist's CPS report. The accuracy of CPS was evaluated by the difference between expected C_(peak) and measured C_(peak). Then the speed and the accuracy of CPS were expressed as σ level. After the campaign for improvement by Six Sigma method, the speed was improved by 1.19σ (before and after the campaign; 0.56σand 1.75σ, respectively) and the accuracy was improved by 0.7σ (before and after the campaign, -0.56σand 0.14σ, respectively). Exact recordings of dosing time, drug infusion hours and blood sampling time were shown to have profound effect on the accuracy of CPS. The improvement in the speed and the accuracy of CPS could produce potential financial benefit of U.S.$ 15,300 a year. There have not been studies on evaluating and objectifying as numerical value for the speed and the accuracy of CPS; thus, 6-Sigma method for improving efficiency in manufacturing process was applied to CPS process by expressing as σlevel. The speed and the accuracy of CPS could be improved by tightly controlling many factors related to CPS efficiency.