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- 저자 : Youming Hou (검색결과 3 건)
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( Habib Ali ),( Abrar Muhammad ),( Youming Hou ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.5
The intracellular bacterium Wolbachia pipientis is widespread in arthropods. Recently, possibilities of novel Wolbachia-mediated hosts, their distribution, and natural rate have been anticipated, and the coconut leaf beetle Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), which has garnered attention as a serious pest of palms, was subjected to this interrogation. By adopting Wolbachia surface protein (wsp) and multilocus sequence type (MLST) genotypic systems, we determined the Wolbachia infection density within host developmental stages, body parts, and tissues, and the results revealed that all the tested samples of B. longissima were infected with the same Wolbachia strain (wLog), suggesting complete vertical transmission. The MLST profile elucidated two new alleles (ftsZ-234 and coxA-266) that define a new sequence type (ST-483), which indicates the particular genotypic association of B. longissima and Wolbachia. The quantitative real-time PCR analysis revealed a higher infection density in the eggs and adult stage, followed by the abdomen and reproductive tissues, respectively. However, no significant differences were observed in the infection density between sexes. Moreover, the wsp and concatenated MLST alignment analysis of this study with other known Wolbachia-mediated arthropods revealed similar clustering with distinct monophyletic supergroup B. This is the first comprehensive report on the prevalence, infection dynamics, and phylogeny of the Wolbachia endosymbiont in B. longissima, which demonstrated that Wolbachia is ubiquitous across all developmental stages and distributed in the entire body of B. longissima. Understanding the Wolbachia infection dynamics would provide useful insight to build a framework for future investigations, understand its impacts on host physiology, and exploit it as a potential biocontrol agent.
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Mate recognition and antennal morphology of Octodonta nipae (Coleoptera: Chrysomelidae) adults
Lingfei Peng,Limin Chen,Jinlei Li,Youming Hou,Yigen Chen 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
The nipa palm hispid beetle, Octodonta nipae (Maulik) has been killing palm trees since its introduction intoHainan province, China, from Malaysia in 2001. It continues to spread within Hainan province, northeast toFujian province, and northwest to Yunnan province within China. Knowledge on signals involved in mate locationand recognition could help develop effective integrated pest management programs. In the present study,we first experimentally proved that antennae were essential in success of O. nipae mating. We then excisedvarious segments/flagellomeres of adult male and female antennae and observed their mating behavior. Resultsrevealed that the 5th to 9th flagellomeres, especially those of males, were important for the mating success. Finally, in an attempt to elucidate the types of antennal sensilla accountable for the mating success, morphologyof O. nipae antennae was studied in detail with scanning electron microscopy. Six types of sensilla were distinguished:aporous sensilla trichodea (T1), multiporous sensilla trichodea (T2), aporous sensilla chaetica (Ch1),uniporous sensilla chaetica (Ch2), multiporous sensilla basiconica (B), and Böhm sensilla (Bm). Aporous sensillatrichodea is the most abundant; multiporous sensilla trichodea and sensilla basiconica are considered as olfactoryreceptors, and uniporous sensilla chaetica as gustatory receptor. Importance of flagellomeres 5–9 in matingsuccess seemed to correspond to the abundance of sensilla on these segments.
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Hong Chang,Jiang Long Guo,Xiao Wei Fu,Xiu Jing Shen,Youming Hou,Kongming Wu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Cryptochrome (CRYs) proteins have been elucidated as the molecular basis for magnetoreception in Drosophila, and a putative magnetic receptor (named IscA1) protein may aslo be involved in sensing magnetic fields in Drosophila. However, whether IscA1 has a conserved role in diverse animals and functions in orientation during animal migration is unknown. Here we report on the cloning and sequencing of the IscA1 gene from Agrotis segetum, which encodes a predicted protein IscA1 that has 131 amino acids and two conserved iron-sulphur cluster binding domains. Multiple sequence alignment and phylogenetic analysis were used to show that IscA1 had a relatively high homology from species of Noctuoidea. Quantitative polymerase chain reaction showed that IscA1 was ubiquitously expressed in adult organs and, among all developmental stages, expression was higher in adults. When Agrotis segetum was exposed to 14 h light/10 h dark, IscA1 expression also showed daily oscillations, and constant light or dark disturbed these oscillations. IscA1 expression levels in a migratory population were higher than in a reared population and higher in a southward migratory population than in a northward. These findings suggest that the IscA1 gene in A. segetum might be associated with migration and provide a molecular basis for further study on the functions of IscA1 gene in magnetoreception and potential control of the turnip moth.
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