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Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming
Marí,n, Marí,a del Carmen,Agathangelou, Damianos,Orozco-Gonzalez, Yoelvis,Valentini, Alessio,Kato, Yoshitaka,Abe-Yoshizumi, Rei,Kandori, Hideki,Choi, Ahreum,Jung, Kwang-Hwan,Haacke, Stefan American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.1
<P>The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium <I>Anabaena</I> PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.</P> [FIG OMISSION]</BR>
Kise Yoshitaka,Kuwada Chiaki,Mori Mizuho,Fukuda Motoki,Ariji Yoshiko,Ariji Eiichiro 대한영상치의학회 2024 Imaging Science in Dentistry Vol.54 No.1
Purpose: The aims of this study were to create a deep learning model to distinguish between nasopalatine duct cysts (NDCs), radicular cysts, and no-lesions (normal) in the midline region of the anterior maxilla on panoramic radiographs and to compare its performance with that of dental residents. Materials and Methods: One hundred patients with a confirmed diagnosis of NDC (53 men, 47 women; average age, 44.6±16.5 years), 100 with radicular cysts (49 men, 51 women; average age, 47.5±16.4 years), and 100 with normal groups (56 men, 44 women; average age, 34.4±14.6 years) were enrolled in this study. Cases were randomly assigned to the training datasets (80%) and the test dataset (20%). Then, 20% of the training data were randomly assigned as validation data. A learning model was created using a customized DetectNet built in Digits version 5.0 (NVIDIA, Santa Clara, USA). The performance of the deep learning system was assessed and compared with that of two dental residents. Results: The performance of the deep learning system was superior to that of the dental residents except for the recall of radicular cysts. The areas under the curve (AUCs) for NDCs and radicular cysts in the deep learning system were significantly higher than those of the dental residents. The results for the dental residents revealed a significant difference in AUC between NDCs and normal groups. Conclusion: This study showed superior performance in detecting NDCs and radicular cysts and in distinguishing between these lesions and normal groups.
Microstructure Control of Grain-oriented Barium-titanate Ceramics and Their Piezoelectric Properties
Petr Pulpan,Rintaro Mori,Hiroshi Hayashi,Yoshitaka Nagamori,Yuichi Yamamoto,Satoshi Wada 한국물리학회 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.57 No.41
A templated grain growth (TGG) method was used for the preparation of grain-oriented bariumtitanate (BaTiO3, BT) ceramics. [110]-oriented BT plate-like template particles were dispersed in a matrix of hydrothermal BT spherical particles of various sizes. The Lotgering method using the Xray diffraction (XRD) pattern determined the degree of orientation along the [110] direction (F110). An optimization of the preparation conditions was performed in terms of the template-to-matrix weight ratio (T/M), the Ba/Ti ratio, and the sintering temperature and time in order to achieve a high F110 along with a small grain size and a high density. A grain size as small as 10 µm could be reached for Ba/Ti ratios close to the stoichiometric value. Lower values led to an increase in F110 and in the grain size. A T/M bigger than 80% was necessary for F110 to be above 80% and for the grain size lower than 40 µm. The density was almost independent of the T/M and the Ba/Ti ratios. The grain growth was limited by a two-step sintering process. Small grain size, high F110 and high density are essential if the piezoelectric constant d33 is to be increased above the values for standard BT ceramics.
Interventional management for postoperative arterial bleeding in gastrointestinal surgery
Yozo Sato,Kiyoshi Matsueda,Marie Osawa,Yoshitaka Inaba,Yu Takahashi,Yosuke Inoue,Atsushi Oba,Yosuke Fukunaga,Yasuhiro Shimizu 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.4
Postoperative arterial bleeding after gastrointestinal surgery is a potentially fatal complication. Transcatheter arterial embolization is considered the first-line treatment because of efficacy and less invasiveness despite the risk of organ infarction. With the recent advances in endovascular devices, stent-graft placement, which can preserve arterial flow, has been an alternative treatment option in patients with extrahepatic artery hemorrhage. Moreover, clinical outcomes of stent-graft placement for pseudoaneurysms in relative long term have been reported recently. Herein, we review the techniques and clinical outcomes for interventional management for postoperative arterial bleeding.
Interventional management for postoperative arterial bleeding in gastrointestinal surgery
Yozo Sato,Kiyoshi Matsueda,Marie Osawa,Yoshitaka Inaba,Yu Takahashi,Yosuke Inoue,Atsushi Oba,Yosuke Fukunaga,Yasuhiro Shimizu 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.4
Postoperative arterial bleeding after gastrointestinal surgery is a potentially fatal complication. Transcatheter arterial embolization is considered the first-line treatment because of efficacy and less invasiveness despite the risk of organ infarction. With the recent advances in endovascular devices, stent-graft placement, which can preserve arterial flow, has been an alternative treatment option in patients with extrahepatic artery hemorrhage. Moreover, clinical outcomes of stent-graft placement for pseudoaneurysms in relative long term have been reported recently. Herein, we review the techniques and clinical outcomes for interventional management for postoperative arterial bleeding.
Differences in the panoramic appearance of cleft alveolus patients with or without a cleft palate
Fujii Takeshi,Kuwada Chiaki,Kise Yoshitaka,Fukuda Motoki,Mori Mizuho,Nishiyama Masako,Nozawa Michihito,Naitoh Munetaka,Ariji Yoshiko,Ariji Eiichiro 대한영상치의학회 2024 Imaging Science in Dentistry Vol.54 No.1
Purpose: The purpose of this study was to clarify the panoramic image differences of cleft alveolus patients with or without a cleft palate, with emphases on the visibility of the line formed by the junction between the nasal septum and nasal floor (the upper line) and the appearances of the maxillary lateral incisor. Materials and Methods: Panoramic radiographs of 238 patients with cleft alveolus were analyzed for the visibility of the upper line, including clear, obscure or invisible, and the appearances of the maxillary lateral incisor, regarding congenital absence, incomplete growth, delayed eruption and medial inclination. Differences in the distribution ratio of these visibility and appearances were verified between the patients with and without a cleft palate using the chi- square test. Results: There was a significant difference in the visibility distribution of the upper line between the patients with and without a cleft palate (p<0.05). In most of the patients with a cleft palate, the upper line was not observed. In the unilateral cleft alveolus patients, the medial inclination of the maxillary lateral incisor was more frequently observed in patients with a cleft palate than in patients without a cleft palate. Conclusion: Two differences were identified in panoramic appearances. The first was the disappearance (invisible appearance) of the upper line in patients with a cleft palate, and the second was a change in the medial inclination on the affected side maxillary lateral incisor in unilateral cleft alveolus patients with a cleft palate.