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      • KCI등재

        Breeding Hybrid Rice with Genes Resistance to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

        김미선,Sothea Ouk,Kuk-Hyun Jung,Yoohan Song,Le Van Trang,Ju-Young Yang,조용구 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.3

        Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

      • SCIESCOPUSKCI등재

        Ecology and Behavior Overwintering Two Aphid Species, Lipaphis pseudobrassicae and Myzus persicae(Homoptera: Aphididae), in Southern Greenhouse Area in Korea

        Vuong, Pham-Thi,Kim, Jinho,Yoohan Song Korean Society of Applied Entomology 2003 Journal of Asia-Pacific Entomology Vol.6 No.1

        In southern Korea, the two aphid species, Myzus Pessicae(Sulzer) and Lipaphis Pseudobrassicae (Davis), had been known to have sexual phase to produce hibernating eggs on their alternate hosts. However, we have fequently found that they were still alive in the parthenogenetic form even in below freezing conditions in winter. This study had been focused on verifying the possibility of overwintering of the two important aphid species in the parthenogenetic morph, by comparing the population density changes within greenhouse and open field condition. In green- house condition, though not heated, both species successfully continued reproducing in a parthenogenetic form. In open field, L. pseudobrassicae successfully survived and reproduced on chinese cabbage and spinach even below freezing temperatures. M. persicae were also able to survive in the winter but not as much capability of reproduction as L. preudobrassicae. This study provided the evidence that the aphid infestations in greenhouse can be mostly from overwintering populations in the parthenogenetic morph in southern greenhouse area of Korea.

      • SCIESCOPUSKCI등재

        The Seasonal Occurrence of the Two Aphid Species, Myzus persicae and Aphis gossypii, and their Natural Enemies on Vegetable Crops in Chinju, Korea

        Vuong, Pham-Thi,Kim, Jinho,Yoohan Song Korean Society of Applied Entomology 2001 Journal of Asia-Pacific Entomology Vol.4 No.1

        We conducted a study on the population dynamic of the two major aphid species of green peach aphid, Myzus persicae(Sulzer), and cotton aphid, Aphis gossypii (Glover), as well as their natural enemies on the five major green house crops in Chinju greenhouse vegetable area in Korea. The green peach aphid was relatively the majority among all aphids collected from tomato, potato, and chinese cabbage, while the cotton aphid was major species on water melon and hot pepper. Both species reached their peaks in mid June, when the summer monsoon was not initiated, the temperature was relatively as low as 23$^{\circ}C$, and the crops were young and favorable. the density of predators such as spiders, lady beetles and green lacewings and some other arthropods were very low in the early season and reached their pick three weeks after the aphids density peaked. This data indicated that the predators only follow aphid abundance but arrive too late to be effective as regulators of aphids population. About 9,255 samples of various aphids collected from the five crops, we found 39 parasitoids representing at least eight different species. Aphidiid sp. occupied 35.8% and hyperparasites occupied 56.5%. Lipolexis scutellaris species was ver scarce and only occupied 2.6% from Aphis gossypii on watermelons. This information can be used for determining the impact of natural enemies under the mixture of greenhouse-open field vegetable production system.

      • KCI등재

        Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

        ( Me-sun Kim ),( Sothea Ouk ),( Kuk-hyun Jung ),( Yoohan Song ),( Le Van Trang ),( Ju-young Yang ),( Yong-gu Cho ) 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.3

        Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

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