N-(benzoyl)-C-(N-methylanilino)imidoylchloride 유도체의 가수분해 반응메카니즘

權奇星,李龍九,成洛道,金千石 충남대학교 기초과학연구소 1993 연구논문집 Vol.13 No.-

25℃의 50%(v/v) 메탄올-물의 혼합용매 속에서 pH 변화에 따른 X-치환된 N-(benzoyl)-C-(N-methylanilino)imidoylchloride(s)들의 가수분해 반응속도상수를 측정하여 반응속도식, 치환기효과, 용매효과, 염효과, 열역학적 활성화 파라미터 및 가수분해반응 생성물 분석 등의 결과로부터 pH3.0∼10.0까지의 사이에서는 azocarbonium ion 중간체를 지나는 S_N1형 반응, pH3.0 이하와 pH10.0 이상의 pH에서는 사면체 중간체를 지나는 친핵성 첨가-제거반응(Ad_N-E)이 일어남을 제안하였다. Rate constants of hydrolysis of N-(benzoyl)-C-(N-methyl-anilino)imidylchlorides were determined by UV spectrophotometry in 50% (v/v) aqueous methanol at 25℃. On the basis of rate equation, substituent effect, solvent effect, salt effect, thermodynamic parameters and hydrolysis product analysis, it may be concluded that the hydrolysis of N-(benzoyl)-C-(N-methyl-anilino)imidylchlorides proceed through S_N1 mechanism via azocarbonium ion intermediate in the range of from pH 3.0 to pH 10.0, while above pH 10.0 and below pH 3.0 the hydrolysis proceeds through nucleophilic addition-elimin ation (Ad_N-E) mechanism.

Phenyl-N-benzoylchlorothioformimidate 誘導體의 加水分解 反應 메카니즘

權奇星,金千石,李龍九,成洛道 충남대학교 기초과학연구소 1992 연구논문집 Vol.12 No.-

25℃의 30%(v/v) dioxane-물의 혼합용매 속에서 pH 변화에 따른 X와 Y-치환된 phenyl-N-benzoylchlorothioformimidates(S)들의 가수분해 반응 속도상수를 측정하여 반응속도식을 유도하고, 경계 궤도 함수 상호작용, 용매효과, 일반 염기 촉매효과, 열 역학적 활성화 파라미터, 및 가수분해반응 생성물 분석 등의 결과로부터 pH 10.0 이하의 낮은 pH에서는 azocarbocation 중간체를 지나는 S_N1형 반응, pH 11.0 이상의 높은 pH에서는 사면체 중간체를 지나는 친핵성 첨가-제거(Ad_N-E)반응 그리고 pH 10.0과 11.0 사이에서는 이들 두가지 유형의 궤도 조절 반응이 서로 경쟁적으로 일어남을 제안하였다. The rate constants of hydrolysis of phenyl-N-benzoylchlorothioformimidates were determined by UV. spectrophotometry in 30% (v/v) aqueous dioxane at 25℃. On the basis of rate equation, general base catalysis, solvent effect, substituent effect, thermodynamic parameters, frontier orbital interaction and hydrolysis product analysis, it may be concluded that the hydrolysis of phenyl-N-benzoylchlorothioformimidates proceed through S_N1 mechanism via azocarbocation intermidiate below pH 10.0, while above pH 10.00 the hydrolysis proceeds through nucleophilic addition-elimination (Ad_N-E) mechanism. In the range of pH from 10.0 to 11.0 these two reaction occur competitavely.

악교정 환자의 악교정 수술전후 발음양상에 대한 비교연구

권경환,김수남,이동근,조용민,이숙향 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

The purpose of this study was undertaken to determine the effects of orthognathic surgery on speech. The hyposis stated herein is that functional behaviors of the dentofacial complex, such as speech production, may be adversely affected by deviations of a structural nature(especially, Class III malocclusion) . Twenty adults with Class III malocclusion(13 female and 7 male) were studied preoperative, immediate postoperative and either 6 or 12 months postoperative lateral cephalograms. They had mandibular prognathism and had undergone mandible setback operation. The position of tongue, soft palate(Uvula), hyoid bone, respiratory track width, and pharyngeal depth were assessed on lateral cephalograms with 23 cephalometric variables, ANOVA, Paired t-tests and Pearson's product-moment correlation coefficient tests were used to evalute the operative changes in all cephalometric parameters. A experienced speech and language pathologists performed narrow phonetic transcriptions of tape-recorded words and sentences produced by each of the ninth patients and the recording tapes were analyzed by phonetic computer program(Computerized Speech Lab(CSL) Model 4300BI(U.S.A.)) These judges also recorded their ratings of each patient's overall consonants, hypernasality, hyponasality, and articulation proficiency. The results obtained are as follows ; 1.There were significant changes in distance of posterior pharyngeal wall to tongue (TI-TW2, TS-TW3) after the surgery at 6 months postoperatively(each p<0.01 p<0.05) 2.The posterior tongue point(TI, TS, PPT) moved posteriorly after surgery and remained to its changed position at 6 months postoperatively(p<0.05) . The displacement of tongue was correlated with the movement of mandibular setback amount(p<0.05). The hyoid bone moved posteriorly superiorly after immediate postoperative period. There was significant changes in hyoid bone movement after immediated post-operative period(p<0.05), but returned to its original position during the follow-up period(p>0.05) 3.The soft palate was displaced posteriorly superiorly after immediated operative period and remained to its changed position at 6 months postoperatively(p<0.05) ANS-PNS-SPT angle increasing, PPU-PPPo distance narrowing was showed after surgery, and remained its appearance 6 months postoperactively (p<0.05) 4.There were significant changes in formant value and squre diagram of vowel sound after the orthognathic surgery and the follow-up period. There were significant changes in /ㅅ/sound and posterior tongue sound. 5.The posterior movement of tongue and the posteriosuperior movement of soft palate was correlated with mandibular setback amount after orthognathic surgery. On the vowel squre diagram, the author found that the place of articulation after operation moved downward, backward, upward. 6.In assessing speech abnormalities, dental occlusion should be considered as a contributing factor. The vast majority of subjects with preoperactive misarticulations eliminated or reduced their errors following orthognathic surgery. There was significant difference in speech impovement between pre- and post-operation.

제지폐수처리용 생물고분자응집제를 생산하는 Bacillus sp. K-111의 배양특성조사

권기석,손용호,최선택,정석관,송숙희,김동걸 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

Flocculant-producing microorganisms were screened from obtained strains in the laboratory using a pulp-wastewater treatment as the flocculating agent. K-111 strain that had high flocculating activity among them was selected and identified as Bacillus sp. K-111 16s rDNA sequencing. The favorable medium for the production of flocculant was glucose 1.5%, NH_4NO_3 0.2%, tryptone 0.01%, K_2HPO_4 0.08%, KH_2PO_4 0.06%, CaCO_3 0.03%, MgSO_4·7H_2O 0.005%, MnSO_4 0.005% in 1 liter of D.W. at initial pH 7.0. The optimum culture temperature and pH were 30℃ and pH 7.0, respectively. the flocculating activity was observed most highly after 36 to 48 hr of cultivation at the optimum conditions. the flocculating activity of produced biopolymer on optimum conditions was about 2.5-fold higher than that of screening medium.

근긴장성 이영양증 환자 1례에 대한 보고

권용욱,조종훈,정필현,강석 東國大學校醫學硏究所 1997 東國醫學 Vol.4 No.-

근긴장성 이영양증은 1909년 Steinert에 의해 처음으로 보고된 질환으로 상염색체 우성유전으로 발현되며 근긴장증 이외에 전신증상이 함께 나타나는데, 전두두 대머리, 긴 얼굴, 안검하수, 역전된 미소모양의 입술등이 보이고 식도, 대장, 자궁 등의 평활근도 침범하여 연하곤란, 구음장애, 변비, 분만장애 등을 일으킬 수 있다. 또한 심장비대와 심장전도의 이상을 동반하는 경우 이로 인한 부정맥이 주요 사망원인이 될 수 있다. 이러한 특징적인 임상증상 외에 근전도 검사상 일명 dive bomber sound 또는 motorcycle sound를 보이는 운동단위전위가 특징적으로 관찰된다. 본 교실에서는 좌측 근위약을 호소하고 원위부보다 근위부 근력이 더 심하게 약화되어 있는 근긴장성 이 영양증 환자 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Myotonic dystrophy, first described by Steinert in 1909, is an autosomal dominant multisystem diease. Most patients note the insidious onset of weakness in the limb muscles or difficulty relaxing muscles after a strong contraction. The evidence of Multisystem involvement includes early frontal balding, less than average intelligence, posterior subcapsular cataracts, abnormal cardiac conduction and testicular tubular cell atrophy. In addition, there is wasting of the facial musculature, including the temporalis, masseter, and stemocleidomastoid muscles, which produces a characteristic long face with ptosis, hollow temples, and a hanging jaw. Electromyography is extremely helpful in making the diagnosis. The presence of myotonic discharges in affected muscles is characteristic finding in electromygraphy. Both the amplitude of the potentials and the frequency of the discharges wax and wane. The sound of the discharges is quite characteristic and in their recognition which is similar to a dive bomber sound or motorcycle sound. We recently experienced a case of myotonic dystrophy who complained unusual symptoms such as the left side weakness, proximal muscle weakness rather than distal muscle weakness. So we report this case with review of the literatures.

방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

권기정,최용석,황의환,이상래,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 7-15)

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

복합열화환경하에서의 중성화 및 염화물이온 확산특성이 철근부식에 미치는 영향에 관한 실험적 연구

조봉석,장재봉,김재환,김용로,권영진,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

It is recognized that reinforcement corrosion is the main distress behind the present concern regarding concrete durability. In this study, to confirm corrosion of reinforced concrete due to permeability of carbonation and chloride ion affected by thickness of cover, kinds of surface coating, measured electric potential, ratio of corrosion area, weight reduction, corrosion velocity of steel bar under environment of complex deterioration. The results showed that an increase in age also decreases Carbonation velocity coefficients and increases Chloride ion diffusion coefficients. As well, an increase in age also increases corrosion of reinforcement due to permeability of carbonation and chloride ion. Corrosion of reinforcement by thickness of cover is superior to 10mm thick than 20mm thick. It showed that an increase in thickness of cover prevent steel bar from deteriorating due to permeability of carbonation and chloride ion. The results of this study showed that corrosion velocity was affected by thickness of cover, kinds of surface coating. data on the development of corrosion velocity made with none, organic B, organic A, inorganic B. and inorganic A is shown.

醫藥分業에 대한 保健所 公務員들의 認識과 態度

박석수,박재용,권진희 경산대학교 보건복지연구소 2000 保健福祉硏究 Vol.5 No.-

This research was conducted to find the attitude of public health officials about the division of roles between doctors and pharmacists (DRDP) which would be committed from July 01, 2000. The survey was carried out for the 600 public health officials from 25 health centers between March 2, 2000 and March 31, 2000 in Kyoungsan-Bukdo area. 33.0% of respondents were highly aware of the DRDP, and 63.0% were moderately aware of the DRDP. The proportions of the respondents who recognized that the health center should include DRDP agency are 43.3% among the health officials, 25.8% among the administrative officials, 80.0% among the drug relating officials, and 60.0% among the temporary officials. 65.3% of respondents answered that exceptions of DRDP should be allowed in some cases, such as, in areas where there is no medical facilities or pharmacies. 58.0% responded that the network system between pharmacies and medical facilities should be necessary so that the exchange of information is allowable in case that the prescribed medicine is not in the pharmacies. 30.0% agreed that the abuse of injections should be prevented, and 41.2% responded that the health center should emphasize public relations when there is any inconvenience to implement the injection. 65.3% were that there is little inconvenience about substitution of preparation of medicine, and 28.2% were that the strengthening of the administrative action by the pharmaceutical affairs law might be useful in order to improve patients convenience. The participants supported that a doctor can prepare patients medicine for an emergency patient (97.7%) and the first infectious disease (96.5%). The participants also supported that a pharmacist can prepare patients medicine for the areas of no medical facilities (79.3%) and disaster areas (78.8%). The participants were affirmatively showed about the system of using community drug sotre (helpful (66.5 percent) and not helpful (33.5 percent)) and the fuction of the primary medical institutions, that is, the family doctor system (helpful (81.8%) and not helpful (18.2%). The participants also recognized that the regional cooperation committees would be helpful to minimize patients inconvenience. Officials participated in this survey responded that education of residents and the public information by consumers cooperative institution would produce the most efficient effect. This study can be concluded that the recognition of health officials varies by the education level, job position level, and primary duty. Therefore, the education of DRDP for health officials is necessary for improvement of their ability and knowledge and for the smooth introduction of the division between medical roles.

전어의 보존중 겨자의 처리 효과

서권일,강갑석,이용수,정용진,김용택,심기환 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

Effects of mustard seed(Brassica juncea) on the preservation of raw gizzard-shad slice were investigated, morphological change of Vibrio parahaemolyticus treated with water-extract of mustard seed was examined, the results are as following. The pH of raw gizzard-shad slice soaked in water with mustard seed slightly increased during preservation, and the pH was in inverse proportion to the added amount of mustard seed. The number of bacteria and Vibrio of raw gizzard-shad slice treated with mustard seed were lower than control, and the higher the mustard seed concentration, the lower the number of bacteria and Vibrio. The TMA-N content of raw gizzard-shad slice gradually increased during preservation, and the TMA-N was in inverse proportion to the added amount of mustard seed. Scanning electron microscopy of Vibrio parahaemolyticus treated with water extract of mustard seed showed that surface membrane was destroyed with expended ellipsoidal shape.