T 세포활성항원 CTLA-4의 기능에 관한 연구 : T 세포에서 표적세포 살해능과 CTLA-4 발현과의 연관성 Target Cytotoxicity of T Cell Correlates with CTLA-4 Production

노만택,조양자,김용식,최용,조보현,최장원,정용훈 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.4

CTLA-4, a T cell activation antigen and a homologue of CD28, was originally identified as a gene by a series of reverse genetic approaches. While CD28 molecule has been characterized well as a stimulator of T-cell function via enhanced lymphokines production and stablilization of those mRNA, most of the functions of CTLA-4 remain unknown. It has been widely accepted that CTLA-4 functions as an immune suppressor which is down-regualting the function of CD28. We previously showed that 34 KD form of this antigen mainly expressed CD8+ subset, a cytotoxic or suppressor, of activated peripheral blood lymphocyte. Based on our previous finding this study was conducted to further characterize immunological function of CTLA-4 especially in terms of involvement of this molecule in T-cell effector function mediating target cell cytotoxicity. 4 human T cell clones with different target cytotoxicities were employed in this study. NBL46 (CD4+) and NBL77 (CD8+) were cytotoxic and NBL32 (CD4+) and NBL58 (CD 8+) were non-cytotoxic to target LBF cell in target cell chromium release assay. And in Western blot assay 34 kD antigen was detected in NBL46 (CD4+) and NBL77 (CD8+) clones but not in NBL32 (CD4+) and NBL58 (CD8+) clones. It was summarized that expression of the CTLA-4 antigen was associated with cytotoxicity but not with subset phenotypes of T cells. In conclusion CD8+ T subset of PHA-stimulated PBL was major and only CTLA-4 producer and this molecule was induced during mid to late stage of T cell activation. The cytotoxicity of T cell clones to its target cell was directly correlated with its CTLA-4 production and vice versa. And it was highly suggested that primary function of CTLA-4 may involved in T cell effector function which mediates target cell killing.

美國醫師들이 처한 法的問題들

盧鎔冕 大韓法醫學會 1990 대한법의학회지 Vol.14 No.1

미국 의사의 지위

노용면 대한의학협회 1980 대한의학협회지 Vol.23 No.12

日本의 金融規制의 歷史的 變遷과 시준점

노택환,최용석 영남대학교 지역발전연구소 1997 새마을지역개발연구 Vol.21 No.-

The purpose of this paper is to survey historical view on financial regulations and institutions of Japan and to show an fundamental implication on financial irregulation policy of Korea. To analysis a case of Japan, We divided time into three, i.e., a period of high-speedy growth(1945-1969), low-speedy growth(1970-1980), and at a later period. Financial regulations in high speedy growth period aimed at regulating competition. Such regulations were finished supporting benefits of financial institutions. After 197Os(Oil Shock), Japan had set in a period of low-speedy growth and former regulations had been unsuitable for new economic environment, i.e., rapid development of the communication facilities and computer system. Due to these development, new financial commodities with high risk had been developed. Such changes had speeded up financial irregulation. Besides, after from 1992 on, the collapse of "Bubble Economy" has accelerated institutional reorganization of financial irregulation. But we are able to say that financial irregulation of Japan was "controled irregulation". In conclusion, we demend introduction of a concept of law and economics. This concept is preventing government from direct control of economic behavior and is needing to make minimal law to constitute a basical frame of markets which is effectively working by invisible hand. This concept of market principle is effective in Korea.

水稻의 適正 用水量 算定에 關한 硏究 : 葉水面 蒸發量을 中心으로 For Amount of Evapotranspiration

黃龍鎭,宋錫銀,鄭鳳守,朴魯奭,李裕根,李永日,黃光性 진주산업대학교 1976 論文集 Vol.14 No.-

1. 試驗기간의 氣象現況은 Table 1과 같다. 2. 벼의 엽면증발량은 活着後 점차 增加하여 第8기에 Peak로 되며 이때의 1日 엽면 증발량은 5.16mm/day로 되었다가 점차 減少現象을 보인다. 3. 엽면증발량은 第8期에 全 葉面증발량의 17.5%에 達한다. 4 벼의 증산비는 282이였다. 5. 株間수면증발량은 Table 3과 같으며 葉面증발량과는 負(一)의 相關關係가 있음을 보였다. 6. 증발계증발량과 엽수면증발량과의 比는 1.43이였다. 7. 증발계증발량과 葉面증발량과의 비는 0.89이였다. 8. Blaney- Criddle method에 依한 k値는 Table 5와 같다. 9. 증산强度는 총계 1021로, 증발산强度는 총계 1141.8로 나타났다. The resuts of the study on consumption use of irrigated water in pabdy field during the growing stage of gaddy rice are summarized as follow. 1. Amount of transpiration of paddy rice increases gradually after trasplantation and reaches peak on 8th growing period and amount of traspiration reached on 5.16mm/day, then it decreases gradual1y after that. 2. Trnspirstion reached l7. 5% of amount of transpiration at 8th growing period. 3. Transdiration ratio of paddy rice approximate1y 262. 4. As we know from Fig. 2 correlation between the amouut of everporation from water surface in paddy field and amount of trans piration shows high negative. 5. Ratio of between evaportion and evapotranspiration is 1.43. 6. Ratio of between evaportion and transpiration is 0.89. 7. The k value of Blaney-Cliddle methed shows Tab1e 6. 8. Transpion and evaoptranspiration intennsities is each other 1021 and 1141.8.本 試驗은 엽수면증발량 株間수면증발량을 實測하였으며 이 試驗에 여러 가지 未備한 点이 있는 것으로 思料되나 以上의 試驗結果를 要約하면 다음과 같다.

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

電子商去來에 따른 租稅政策의 對應方案에 관한 硏究

朴魯慶,孫庸禎 朝鮮大學校 社會科學硏究所 2001 社會科學硏究 Vol.22 No.1

남조류 Microcystis aeruginosa의 증식에 미치는 중금속의 영향에 관한 실험적 연구

정용태,윤정로 順天靑巖大學 1992 論文集-順天靑巖大學 Vol.16 No.-

Microcystis aeruginosa의 증식에 미치는 중금속 첨가실험을 하여 조류증식에 미치는 중금속의 영향과 제거되는 중금속의 량을 조사하였다. 조류의 양은 건조중량법으로 중금속의 양은 원자흡광분석기로 분석하였다. 인제한실험에서 Monod type의 동력학적 해석으로 부터 μ_max=0. 6 day^-1이고 Ks=0. 06 mg/L의 값을 얻었다. 중금속이 저농도로 첨가되면 증식촉진효과를 나타냈고, 한계증식촉진농도 이상이 되면 증식저해를 나타냈다. 한계증식촉진농도는 크롬은 극미량이며, 구리와 카드뮴은 0. 2 ㎎/L이고, 코발트는 0. 4 ㎎/L이었다. Microcystis aeruginosa에 중금속을 첨가하면 증식저해는 비길항적 저해로 나타났고, 증식저해상수는 크롬은 0. 85 ㎎/L, 구리는 3. 23 ㎎/L, 코발트는 10. 53 ㎎/L를 나타냈다. 첨가된 중금속은 구리의 경우 5-10%정도 제거가 되고, 크롬이나 코발트는 거의 제거되지 않았다. The effects of heavy metals on Microcystis aeruginosa and quantity of heavy metals removed were experimentally investigate with adding heavy metals to CB media. The algal mass was determied by suspeded solids deried at 103-105℃ ad heavey metals determined by Atomic Absorption Spectrometry. Under the Phosphate-limiting culture condition, the kinetics coefficient of Monod equation, μ_max and Ks, calculated form the least-square method, were 0. 6 day^-1 and 0. 06 ㎎-P/L. The growth of Microcystis aeruginosa was accelerated at low concentration of heavy metal but inhibited above the critical growth-accelerating concentration. The critical growth-accelerating concentration was on the very low level;Cu and Cd was about 0. 2 ㎎/L and Co was 0. 4 ㎎/L respectively. When adding heavy metals to the culture media of Microcystis aeruginosa, the type of growth inhibition was turned out by noncompectitive-inhibition. The growth inhibition consatnat of Cr, Cu and Co was 0. 85 ㎎/L, 3. 23 ㎎/L and 10. 05 ㎎/L respectively. Cr and Co added to CB media was little removed but Cu removed at 5-10%.

방광암 환자에서 염색체 이상이 예후에 미치는 영향에 대한 세포유전학적 연구

나용길,임재성,윤율로,설종구 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Bladder cancer is the most common type of urinary cancer in Korea. Usually it has an unpredictable natural history with respect to tumor invasion and recurrence. The cytogenetic results and genetic alterations of bladder cancer are thought to be closely related to cancer promotion and progression. To predict the possible relationship among them, we performed the cytogenetic analysis in the bladder cancer patients. The specimens of the 32 patients who underwent operations for the bladder cancer were obtained and cultured. Of them 15 cases were cytogenetically abnormal. There were wide variety of structural and numerical chromosomal aberrations. The most frequently affected chromosomes were 4, 8, 9 and 19 as determined by karyotyping. In our study, as in other studies the more the genes were hyperdiploidy and the more the genetic aberrations were complex, and the cancer was more invasive and the cancer grade was higher. There was frequent loss on chromosome 4, 9, 19 and the appearance of marker chromosomes. Although most amplifications and deletions have been previously described in the literature, our study showed some intriguing and uncommon regions, different from those found in past studies.

의인성 망막열공 : 발생 빈도 및 수술성적 Incidence and Surgical Results

김용백,강재훈,서병로,민욱기 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

We reviewed 61 consecutive eyes recently subjected to vitrectomy to determine the incidence and outcome of iatrogenic retinal tears. Iatrogenic retinal tears occurred in 14 eyes(22%). 14 eyes had 22 iatrogenic retinal tears. The incidence of iatrogenic peripheral tears was 6% and of posterior tears was 16%. Two thirds of tears occurred in the inferior quadrants. 43% of iatrogenic retinal tears occurred in patients with proliferative diabetic retinopathy. The overall anatomic success was achieved in 10 eyes(71%), 9 eyes of which were successfully treated with the initial operations. With a combination of intraocular gas tamponade, laser photocoagulation, cryopexy, or scleral bucking, 71% of iatrogenic retinal tears were successfully treated.