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AFLP 분석을 이용한 배추과 식물의 종속간 분자유전연구
권수진(Soo-Jin Kwon),임기병(Ki-Byung Lim),임명호(Myung-Ho Lim),박지영(Jee-Young Park),김진아(Jin-A Kim),김정선(Jung Sun Kim),이수성(Soo-Seong Lee),박범석(Beom-Seok Park),진용문(Yong-Moon Jin) 한국원예학회 2007 원예과학기술지 Vol.25 No.2
Genetic diversity and relationship in the related plant taxa is a prerequisite for crop improvement. Amplified fragment length polymorphism (AFLP) fingerprinting is one of the powerful and widely used tools, which has found wide spread application in numerous studies ranging from genetic mapping to diversity surveys in plants and other organisms. Genome relationship among diploid and allotetraploid Brassica taxa, and between interspecific hybrid or intergeneric hybrid and their parental species were investigated using AFLP display. The two primer pairs for AFLP reactions and seven EcoRI (+2) and M5eI (+3) primer combinations were used in the measurement of genetic relationships. Genetic similarities among nine accessions were estimated by Nei’s distance equation, and cluster analysis was performed using the unweighed pair group method (UPGMA) in the computer program NTSYS-pc. One intergeneric hybrid between Brassica and Raphanus showed 2n=4x=38 chromosomes, of which Raphanus chromosome segment were translocated with B. rapa chromosome.
새로운 선발 마커 D-아미노산 산화효소 유전자를 이용한 식물 형질전환
임선형,우희종,이시명,진용문,조현석,Lim, Sun-Hyung,Woo, Hee-Jong,Lee, Si-Myung,Jin, Yong-Moon,Cho, Hyun-Suk 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1
Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene. $T_{1}$ tobacco seeds derived $T_{0}$ tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.
환경위해성 평가를 위한 해충저항성 배추의 분자생물학적 특성 검정 및 계통 특이 마커 캐발
임선형,김나영,이시명,우희종,신공식,진용문,조현석,Lim, Sun-Hyung,Kim, Na-Young,Lee, Si-Myung,Woo, Hee-Jong,Shin, Kong-Sik,Jin, Yong-Moon,Cho, Hyun-Suk 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4
유전자변형 작물의 상업화를 위해서는 유전자변형 작물이 식품으로서의 안전성과 환경에 미치는 영향에 관한 평가가 이루어져야한다. 이를 위해 개발자는 유전자변형 작물의 방출이전에 유전자변형 작물 개발에 관한 정보를 제출해야만 한다. 본 연구는 유전자변형 작물의 환경 위해성 평가를 위한 분자생물학적 자료를 제공하고자 수행하였다. 아그로 박테리움 형질전환법을 통하여 해충저항성 CryIAc 유전자가 도입된 배추 형질전환체를 획득한 후, 분자생물학적인 분석을 통하여 유전자의 copy수, 안정성, 식물체내에서의 발현을 확인하였고, T-DNA의 배추게놈내의 인접서열을 분석하였다. T-DNA의 게놈내 삽입 인접서열을 바탕으로 유전자변형 배추를 동정할 수 있는 프라이머를 제작하였고, 이를 이용한 검정방법을 수립하였다. 계통 특이 프라이머를 이용한 해충저항성 배추 후대의 PCR 분석결과와 제초체 처리결과가 서로 일치하였다. 본 연구 결과로 환경위해성 평가를 위한 해충저항성 배추의 분자생물학적인 자료를 획득하였으며, 개발된 프라이머는 해충저항성 배추의 검출을 위하여 유용하게 사용할 수 있음을 확인하였다. Commercialization of genetically modified (GM) plants will be required the assessment of risks associated with the release of GM plants that should include a detailed risk assessment of their impacts in human health and the environment. Prior to GM plant release, applicants should provide the information on GM crops for approval. We carried out this study to provide the molecular data for risk assessment of the GM Chinese cabbage plants with insect-resistance gene, modified CryIAc, which we obtained by Agrobacterium-transformation. From the molecular analysis with GM Chinese cabbage, we confirmed the transgene copy number and stability, the expression of the transgene, and integration region sequences between the transgene and the Chinese cabbage genome. Based on the unique integration DNA sequences, we designed specific primer set to detect GM Chinese cabbage and set up the GM cabbage detection method by qualitative PCR analysis. Qualitative analysis with GM Chinese cabbage progenies analysis was revealed the same as the result of herbicide treatment. Our results provided the molecular data for risk assessment analysis of GM Chinese cabbage and demonstrated that the primer set proposed could be useful to detect GM Chinese cabbage.
Karyotype Analysis and Tandem Repeats in Heterochromatin of Brassica rapa
Ki-Byung Lim(임기병),Tae-Jin Yang(양태진),Jee-Young Park(박지영),Soo-Jin Kwon(권수진),JungSun Kim(김정선),Myung-Ho Lim(임명호),JinA Kim(김진아),Mina Jin(진민아),Yong-Moon Jin(진용문),Seog Hyung Kim(김석형),YP Lim(임용표),JW Bang(방재욱) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5