Cloud monitoring system for assembled beam bridge based on index of dynamic strain correlation coefficient

Yiming Zhao,Danhui Dan,Xingfei Yan,Kailong Zhang 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.26 No.1

The hinge joint is the key to the overall cooperative working performance of the assembled beam bridge, and it is also the weakest part during the service period. This paper proposes a method for monitoring and evaluating the lateral cooperative working performance of fabricated beam bridges based on dynamic strain correlation coefficient indicator. This method is suitable for monitoring and evaluation of hinge joints status between prefabricated girders and overall cooperative working performance of bridge, without interruption of traffic and easy implementation. The remote cloud monitoring and diagnosis system was designed and implemented on a real assembled beam bridge. The algorithms of data preprocessing, online indicator extraction and status diagnosis were given, and the corresponding software platform and scientific computing environment for cloud operation were developed. Through the analysis of real bridge monitoring data, the effectiveness and accuracy of the method are proved and it can be used in the health monitoring system of such bridges.

Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis

( Yiming Kou ),( Mingming Wan ),( Wei Shi ),( Jie Liu ),( Zhilei Zhao ),( Yongqing Xu ),( Wei Wei ),( Bo Sun ),( Feng Gao ),( Linjun Cai ),( Chunlai Jiang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, IFN-γ-producing splenocytes, CD69⁺CD8⁺ T cells, and IFN-γ secretion when compared with bacillus Calmette-Guerin (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more IFN-γ-producing splenocytes and CD69⁺CD8⁺ T cells, and higher levels of IFN-γ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of IFN-γ-producing splenocytes sensitive to CD8⁺ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines (IFN-γ and TNF-α) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

Manganese induces neuroinflammation via NF-κB/ROS NLRP3 pathway in rat brain striatum and HAPI cells

Xinyuan Zhao,Lifeng Yin,Yifan Wu,Muxi Han,Yin Zhuang,Yewen Cong,Yiming Liu,Gang Chen,Junkang Jiang 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.2

Backgrounds: Chronic exposure to excessive Mn can result in neurodegenerative symptoms, whose precise molecular mechanism remains largely unclear. Here, we measured the role and mechanism of NLRP3 in Mninduced neuroinflammation in vivo and vitro. Methods: The effects of Mn on NLRP3 activation were investigated by Westernblot, IHC, immunofluorescence analysis, as well as ELISA. We assessed NF-κB activation through measurement of phosphorylation and nuclear translocation. The mechanisms bywhich Mn induced NLRP3 activation were assessed by specific inhibitors. Results: We found that Mn exposure facilitated the activation of NLRP3 inflammasome to promote the production of IL-1β and IL-18 in dose- and time-dependent manners in HAPI cells. In addition, the NLRP3 inflammasome was also dramatically activated in microglia of rat brain striatum after Mn exposure. We also found increased ROS and NF-κB activation. Notably, the activation of NLRP3 was significantly attenuated by pretreatment with NF-κB and ROS inhibitors. Conclusion: These findings suggest that NLRP3 activation plays an important role in Mn-induced neuroinflammation, and it is associated with NF-κB and ROS.

Nanowire interface doping

Nan PAN,Yiming WU,Siwen ZHAO,Huaiyi DING,Xiaoping WANG,Yi LUO,J. G. HOU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Finite Time Fully Distributed Consensus Control for Multi-agent System With Input Saturation and Limited Communication Resources

Miao Xu,Yiming Fang,Jianxiong Li,Xiaodong Zhao 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.11

A finite-time fully distributed consensus (FTFDC) control problem is studied for a second-order multiagent system (MAS) with input saturation, limited communication resources and external bounded disturbances. Firstly, to deal with the input saturation characteristics, a second-order auxiliary system is designed to obtain the compensation signal. Secondly, because the leader information can only be obtained by a group of agents connected with the leader, a distributed estimator for each agent is designed to estimate the leader information. Then, to save communication resources, an event trigger condition is designed without continuous communication between agents. Based on the estimator and event trigger condition, a FTFDC controller is designed to realize the finite time fully distributed consensus control of MAS under limited communication resources and external bounded disturbances. Finally, the designed controller is verified by simulation on a multi-spacecraft attitude system model.

Tissue distribution of marbofloxacin in pigs after a single intramuscular injection

Fan Yang,Yiming Liu,Zhili Li,Yuqin Wang,Baobao Liu,Zhensheng Zhao,Bianhua Zhou,Guoyong Wang 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

Tissue distribution of marbofloxacin was studied in pigs after a single intramuscular injection at 2.5 mg/kg body weight. Samples of plasma, muscle, liver, kidney, heart, lung, and muscle at the injection site were randomly collected from five pigs at 2, 6, 10, 24, 48, 72, and 96 h after administration. Marbofloxacin concentrations were determined by using high-performance liquid chromatography with ultraviolet detection and were subjected to non-compartmental analysis to obtain kinetic parameters. The elimination half-life (t1/2lz) of marbofloxacin at the injection site was 22.12 h, while those in kidney, plasma, liver, lung, heart, and muscle were 16.75, 21.48, 21.84, 24.00, 24.45, and 28.91 h, respectively. Areas under the concentration-time curve from 0 h to ∞ (AUC0–∞s) were calculated to be 31.17 hㆍmgㆍmL−1 for plasma and 32.97, 33.92, 34.78, 37.58, 42.02, and 98.80 hㆍmgㆍg−1 for heart, muscle, lung, liver, kidney, and injection site, respectively. The peak concentration (Cmax) of marbofloxacin was 1.62 µg/mL in plasma and 1.71, 1.74, 1.86, 1.93, 2.45, and 7.64 µg/g in heart, lung, muscle, kidney, liver, and injection site, respectively. The results show that marbofloxacin was fast absorbed, extensively distributed, and slowly eliminated from pigs after a single intramuscular administration.

A solvent-free and vacuum-free melt-processing method to fabricate organic semiconducting layers with large crystal size for organic electronic applications

Ribierre, Jean-Charles,Li, Zhao,Liu, Xiao,Lacaze, Emmanuelle,Heinrich, Benoî,t,Mé,ry, Stephane,Sleczkowski, Piotr,Xiao, Yiming,Lafolet, Fré,dé,ric,Hashizume, Daisuke,Aoyama, Tet The Royal Society of Chemistry 2019 Journal of materials chemistry. C, Materials for o Vol.7 No.11

<P>We report on an improved melt-processing method to prepare organic semiconducting layers with large crystal size and enhanced charge carrier mobilities. The organic compound used in this work is a solution-processable oligo(<I>p</I>-phenylene vinylene) derivative substituted at both ends with pyrene moieties. Accurate control of the temperature during the recrystallization of this compound from the melt enables the formation of large single crystal monodomains in thin films. The melt-processed organic layer shows higher mobilities in transistor configuration than in spin-coated films, which can be attributed to the presence of large-size crystalline monodomains as evidenced by X-ray diffraction measurements. We also investigated the photophysical properties of this material in spin-coated and melted films and found an increase of the photoluminescence quantum yield with the size of the crystals in the organic layer. The advantage of this method over the spin coating also allowed observation of amplified spontaneous emission that was only achieved in the melted film due to its improved luminescence efficiency. Overall, this study demonstrates a simple and versatile method, which does not require the use of any solvent and vacuum, to fabricate organic layers with large crystal size, suitable for the realization of organic electronic and light-emitting devices.</P>

Targeting cathepsin L in the regulation of apoptosis in peripheral T-cell lymphoma

Zhang Rui,Ruan Yanjie,Zhao Yiming,Jin Fengbo,Yang Mingzhen,Zhai Zhimin 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.3

Backgrounds Peripheral T-cell lymphoma (PTCL) is a heterogeneous lymphoproliferative neoplasms derived from post-thymic T lymphocytes and mature NK cells. Although the incidence is rare, PTCL is frequently recurrence with pore prognosis. Cathepsin L is a member of cysteine proteases strongly related to the carcinogenesis and progression of malignant tumors. Objectives To test the expression of cathepsin L in PTCL and to clarify the possible role of cathepsin L in the pathogenesis of PTCL. Results The public datasheets showed a significant increase of cathepsin L in PTCL than normal T-cells. This result was confirmed by the collected tumor samples, in which the pathological changes and the immunohistochemical test were consistent with PTCL diagnosis. Two PTCL cell lines, Jurkat and Hut78, with a stable knockdown of cathepsin L were successfully established. The RNAseq analysis reported a total of eight co-differentially expressed genes between cathepsin Llow Jurkat vs vector Jurkat and cathepsin Llow Hut78 vs vector Hut78 two datasheets. The apoptosis array reported abnormal expression of several pro-apoptosis proteins including Fas, Bax, sTNF-R1, IGFBP-6, etc. According to the gene function enrichment analysis, p53 signaling pathway was involved in loss of cathepsin L in PTCL cells in vitro. In addition, the levels of above differentially expressed genes/proteins were finally checked, and our data confirmed an alteration of p53, apoptosis regulation-related factors (PAK4, SESN2) and apoptosis-related factors (Fas, Bax, sTNF-R1, and IGFBP-6). Conclusion Taken together, we demonstrated a role of cathepsin L in apoptosis by regulation of p53 signaling pathway. Our finding might provide a new possible candidate drug target for clinical treatment of PTCL in future. Backgrounds Peripheral T-cell lymphoma (PTCL) is a heterogeneous lymphoproliferative neoplasms derived from post-thymic T lymphocytes and mature NK cells. Although the incidence is rare, PTCL is frequently recurrence with pore prognosis. Cathepsin L is a member of cysteine proteases strongly related to the carcinogenesis and progression of malignant tumors. Objectives To test the expression of cathepsin L in PTCL and to clarify the possible role of cathepsin L in the pathogenesis of PTCL. Results The public datasheets showed a significant increase of cathepsin L in PTCL than normal T-cells. This result was confirmed by the collected tumor samples, in which the pathological changes and the immunohistochemical test were consistent with PTCL diagnosis. Two PTCL cell lines, Jurkat and Hut78, with a stable knockdown of cathepsin L were successfully established. The RNAseq analysis reported a total of eight co-differentially expressed genes between cathepsin Llow Jurkat vs vector Jurkat and cathepsin Llow Hut78 vs vector Hut78 two datasheets. The apoptosis array reported abnormal expression of several pro-apoptosis proteins including Fas, Bax, sTNF-R1, IGFBP-6, etc. According to the gene function enrichment analysis, p53 signaling pathway was involved in loss of cathepsin L in PTCL cells in vitro. In addition, the levels of above differentially expressed genes/proteins were finally checked, and our data confirmed an alteration of p53, apoptosis regulation-related factors (PAK4, SESN2) and apoptosis-related factors (Fas, Bax, sTNF-R1, and IGFBP-6). Conclusion Taken together, we demonstrated a role of cathepsin L in apoptosis by regulation of p53 signaling pathway. Our finding might provide a new possible candidate drug target for clinical treatment of PTCL in future.

Effects of Acute Sacral Neuromodulation at Different Pulse Widths on Bladder Overactivity in Pigs

Huiling Cong,Limin Liao,Yiming Wang,Lingna Zhao,Zhaoxia Wang,Guang Fu,Guoqing Chen 대한배뇨장애요실금학회 2019 International Neurourology Journal Vol.23 No.2

Purpose: Sacral nerve stimulation has been used to treat overactive bladder. This study evaluated the effects of stimulation using different pulse widths on the inhibition of bladder overactivity by sacral nerve stimulation (SNM) in pigs. Methods: Implant-driven stimulators were used to stimulate the S3 spinal nerve in 7 pigs. Cystometry was performed by infusing normal saline (NS) or acetic acid (AA). SNM at pulse widths of 64 μsec to 624 μsec was conducted at the intensity threshold at which observable perianal and/or tail movement was induced. Multiple cystometrograms were performed to determine the effects of different pulse widths on the micturition reflex. Results: AA-induced bladder overactivity reduced the bladder capacity to 46.9%±7.1% of the NS control level (P<0.05). During AA infusion, SNM at 64 μsec, 204 μsec, and 624 μsec increased the bladder capacity to 126.1%±6.9%, 129.5%±7.3%, and 140.1%±7.6% of the AA control level (P<0.05). No significant differences were found among the results obtained using pulse widths of 64 μsec, 204 μsec, and 624 μsec (P>0.05). The actual intensity threshold varied from 0.7 to 8 V. The mean intensity threshold (T visual) for pulse widths of 64 μs, 204 μs, and 624 μs were 5.64±0.76 V, 3.11±0.48 V, and 2.52±0.49 V. T visual for pulse widths of 64 μsec was larger than the other two T visual for pulse widths of 204 μsec and 624 μsec (P<0.05). No significant differences were found among the T visual for pulse widths of 204 μsec and 624 μsec (P>0.05). Conclusions: This study indicated that different pulse widths could play a role in inhibiting bladder overactivity. It is not yet certain which pulse widths increased bladder capacity compared with AA levels, to minimize energy consumption and maintain patient comfort during stimulation, 204 μsec may be an appropriate pulse width for SNM.

A Tower-Shaped Three-Dimensional Piezoelectric Energy Harvester for Low-Level and Low-Frequency Vibration

Xiaoxiang Wei,Haibo Zhao,Junjie Yu,Yiming Zhong,Yanlin Liao,Shiwei Shi,Peihong Wang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.5

The multiple forms of vibration exist in an ambient environment diffusely and already become a considerable object for energy harvesting. However, how to effectively extract low-level, low-frequency, and multi-directional vibration from the ambient environment is becoming a key issue in the field of energy harvesting. To solve this issue, a tower-shaped piezoelectric vibration energy harvester (TS-PVEH) is reported. Finite element simulation indicates that TS-PVEH works in two fundamental modes, i.e., its in-plane and out-of-plane vibration modes. Meanwhile, simulation results show that the natural frequency of TS-PVEH is 3.39 Hz, 3.40 Hz, and 11.50 Hz, respectively; and the experiments also verified that. By virtue of the tower structure of TS-PVEH, the device is pretty sensitive to three-dimensional vibration. At a low level of acceleration 1 m/s 2 , the maximum load power of TS-PVEH is 65.8 μW in out-of-plane mode and 17.2 μW in in-plane mode, respectively. Furthermore, the effects of the PVDF connection mode on the output performance of TS-PVEH were studied in detail, and comparative experimental results show that a reasonable connection of PVDF can improve energy harvesting efficiency. The proposed TS-PVEH is expected to be used to scavenge energy from multi-dimensional, low-level, and low-frequency vibrations that present in an ambient environment.