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        Experimental Study and Finite Element Analysis of Initial Imperfection on 7A04-T6 Aluminum Alloy Circular Hollow Section Column

        Hui Xu,Yichun Zhang,Bin Rong 한국강구조학회 2021 International Journal of Steel Structures Vol.21 No.3

        In this study, a combination of experiments and fi nite element analyses was conducted to investigate the eff ect of initial imperfections on the axial compression of the 7A04-T6 aluminum alloy columns. Prior to the loading tests, measurement of the initial geometric imperfections for the columns were performed by optical theodolite and vernier calipert. The failure modes of 10 extruded circular columns were investigated. Meanwhile, the ultimate strengths, defl ections and surface strains in whole process under axial compression were recorded. The fi nite element (FE) models of the tested columns were developed using the non-linear fi nite element analysis (FEA) software ABAQUS, and the imperfections and material nonlinearities were considered in the FE models. The validation of the FE models was performed against the test results; it was observed that the proposed FE models are suffi ciently accurate to predict the ultimate strengths and buckling behaviors of the tested columns.

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        Using tyrosinase as a tri-modality reporter gene to monitor transplanted stem cells in acute myocardial infarction

        Mei Liu,Yichun Wang,Mengting Li,Hongyan Feng,Qingyao Liu,Chunxia Qin,Yongxue Zhang,Xiaoli Lan 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

        The study aimed to investigate the feasibility of noninvasive monitoring of bone marrow mesenchymal stem cells (MSCs) transduced with the tyrosinase reporter gene for acute myocardial infarction (AMI) with photoacoustic imaging (PAI), magnetic resonance imaging (MRI), and positron emission tomography (PET) in vitro and in vivo. MSCs were transduced with a lentivirus carrying a tyrosinase reporter gene. After transduction, the rate of 18F-5-fluoro-N-(2- [diethylamino]ethyl)picolinamide (18F-5-FPN) uptake was measured. PAI and MRI of stable cell lines expressing tyrosinase (TYR-MSCs) were performed in vitro. An AMI model was induced and verified. TYR-MSCs and MSCs were injected into the margins of the infarcted areas, and PAI, MRI, and PET images were acquired 1, 7, 14, 21, and 28 days after cell injection. Sham-operated models without injection were used as the control group. TYR-MSCs showed noticeably higher uptake of 18F-5-FPN and stronger signals in T1-weighted MRI and PAI than non-transduced MSCs. In vivo studies revealed prominent signals in the injected area of the infarcted myocardium on PAI/MRI/PET images, whereas no signal could be seen in rats injected with non-transduced MSCs or sham-operated rats. The uptake values of 18F-5-FPN in vivo showed a slight decrease over 28 days, whereas MRI and PAI signal intensity decreased dramatically. MSCs stably transduced with the tyrosinase reporter gene could be monitored in vivo in myocardial infarction models by PET, MRI, and PAI, providing a feasible and reliable method for checking the viability, location, and dwell time of transplanted stem cells.

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