Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

Flatbands and Emergent Ferromagnetic Ordering in Fe3Sn2 Kagome Lattices

Lin, Zhiyong,Choi, Jin-Ho,Zhang, Qiang,Qin, Wei,Yi, Seho,Wang, Pengdong,Li, Lin,Wang, Yifan,Zhang, Hui,Sun, Zhe,Wei, Laiming,Zhang, Shengbai,Guo, Tengfei,Lu, Qingyou,Cho, Jun-Hyung,Zeng, Changgan,Zhan American Physical Society 2018 Physical Review Letters Vol.121 No.9

Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis

Yi Wang,Lin Lin,Chunquan Zheng 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.3

Orai1 is the key subunit of the Ca2+-release-activated Ca2+ channel. Our previous report has demonstrated that Orai1 expression in the airway was upregulated in the ovalbumin (OVA)-induced allergic rhinitis (AR)mouse models. To observe whether inhibition of Orai1expression in the airway could suppress symptoms in a murine model of AR and to assess the impacts of this inhibition on the responses of local and systemic immunocytes,we administered recombinant lentivirus vectors that encoded shRNA against ORAI1 (lenti-ORAI1)into the nostrils of OVA-sensitized mice before the challenges, and analyzed its effect on allergic responses,as compared with the unsensitized mice and untreated AR mice. Administration of lenti-ORAI1 into the nasal cavity successfully infected cells in the epithelial layer of the nasal mucosa, and significantly decreased the frequencies of sneezing and nasal rubbing of the mice. Protein levels of leukotriene C4, OVA-specific IgE, and IL-4 in the nasal lavage fluid and serum and eosinophil cation protein in the serum were also significantly reduced by lenti-ORAI1, as were the mRNA levels of these factors in the nasal mucosa and spleen. These data suggested that administration of lenti-ORAI1 into the nasal cavity effectively decreased Orai1 expression in the nasal mucosa, alleviated AR symptoms, and partially inhibited the hyperresponsiveness of the local and systemic immune cells including T cells, B cells, mast cells and eosinophils that are involved in the pathogenesis of AR.

Different fates of oocytes with DNA double-strand breaks in vitro and in vivo

Lin, Fei,Ma, Xue-Shan,Wang, Zhen-Bo,Wang, Zhong-Wei,Luo, Yi-Bo,Huang, Lin,Jiang, Zong-Zhe,Hu, Meng-Wen,Schatten, Heide,Sun, Qing-Yuan Taylor Francis 2014 Cell Cycle Vol.13 No.17

New damage localization indicator based on curvature for single-span beams

Yi-lin Wang 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.6

Most existing damage detection methods based on curvature should investigate the indicator value changes between the intact and damaged state. However, the ‘footprint’ in the intact state is usually not available for most actual beams. Therefore, a new damage localization indicator called Difference of Nearby Difference Curvature Indicator (DNDCI) was introduced for single-span beams. This indicator does not require prior information of the intact beam and is sensitive to the damage occurs on or nearby the measuring points. Practical and detailed application method of DNDCI has been proposed. Besides the idealized simply supported beams, it was validated by example that DNDCI is also applicable to the actual single-span beams supported by bearings.

Synthesis of mesoporous SiO2 xerogel/chitosan mixed-matrix membranes for butanol dehydration

Yi-Feng Lin,Chang-Yu Wu,Ting-Yu Liu,Kun-Yi Andrew Lin,Kuo-Lun Tung,Tsair-Wang Chung 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-

Novel mesoporous SiO2 xerogel/chitosan (CS) mixed-matrix membranes (MMMs) were successfully prepared by incorporating sol–gel synthesized SiO2 xerogels into glutaraldehyde (GA) cross-linked CS membranes. The effects of the SiO2 doping amount and the feed temperature on the performance of the separation of butanol and water was also investigated in this study. The 0.25 wt% SiO2 xerogel/CS MMMs possessed the best pervaporation performance and the largest PSI value of 1102 kg/m2h with a permeate flux of 736 g/m2h (Permeability of 3.25 × 10−5 g m m−2 h −1 Pa−1) and a separation factor of 1498 at a feed temperature of 50 °C. This PSI value is not only comparable but also exceeds that of other membranes reported in the literature. The separation factor and the flux of the SiO2 xerogel/CS MMMs in this study clearly exceed the upper limit of the previously reported MMMs. As a result, the as-prepared novel SiO2 xerogel/CS MMMs showed an improved performance during the butanol and water separation process, which raises the possibility of future pervaporation applications using this novel SiO2 xerogel/CS MMMs.

Comparative studies of density functionals in modelling hydrogen bonding energetics of acrylamide dimers

Lin, Yi-De,Wang, Yi-Siang,Chao, Sheng D. Techno-Press 2017 Coupled systems mechanics Vol.6 No.3

Intermolecular interaction energies and conformer geometries of the hydrogen bonded acrylamide dimers have been studied by using the second-order Møller-Plesset (MP2) perturbation theory and the density functional theory (DFT) with 17 density functionals. Dunning's correlation consistent basis sets (up to aug-cc-pVTZ) have been used to study the basis set effects. The DFT calculated interaction energies are compared to the reference energy data calculated by the MP2 method and the coupled cluster method at the complete basis set (CCSD(T)/CBS) limit in order to determine the relative performance of the studied density functionals. Overall, dispersion-energy-corrected density functionals outperform uncorrected ones. The ${\omega}B97XD$ density functional is particularly effective in terms of both accuracy and computational cost in estimating the reference energy values using small basis sets and is highly recommended for similar calculations for larger systems.

New damage localization indicator based on curvature for single-span beams

Wang, Yi-lin Techno-Press 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.51 No.6

Most existing damage detection methods based on curvature should investigate the indicator value changes between the intact and damaged state. However, the 'footprint' in the intact state is usually not available for most actual beams. Therefore, a new damage localization indicator called Difference of Nearby Difference Curvature Indicator (DNDCI) was introduced for single-span beams. This indicator does not require prior information of the intact beam and is sensitive to the damage occurs on or nearby the measuring points. Practical and detailed application method of DNDCI has been proposed. Besides the idealized simply supported beams, it was validated by example that DNDCI is also applicable to the actual single-span beams supported by bearings.

Safety and Efficacy of Adalimumab for Patients With Moderate to Severe Crohn`s Disease: The Taiwan Society of Inflammatory Bowel Disease (TSIBD) Study

( Chen Wang Chang ),( Shu Chen Wei ),( Jen Wei Chou ),( Tzu Chi Hsu ),( Chiao Hsiung Chuang ),( Ching Pin Lin ),( Wen Hung Hsu ),( Hsu Heng Yen ),( Jen Kou Lin ),( Yi Jen Fang ),( Horng Yuan Wang ),( 대한장연구학회 2014 Intestinal Research Vol.12 No.4

Background/Aims: Only moderate to severe Crohn`s Disease (CD) patients without a satisfactory conventional therapy effect are eligible to get reimbursement from the National Health Insurance of Taiwan for using adalimumab. These are more stringent criteria than in many Western countries and Japan and Korea. We aim to explore the efficacy of using adalimumab in CD patients under such stringent criteria. Methods: A retrospective analysis was conducted in nine medical centers in Taiwan and we collected the results of CD patients receiving adalimumab from Sep 2009 to Mar 2014. The clinical characteristics, response measured by CDAI (Crohn`s Disease Activity Index), adverse events and survival status were recorded and analyzed. CR-70, CR-100, and CR-150 were defined as attaining a CDAI decrease of 70, 100 or 150 points compared with baseline. Results: A total of 103 CD patient records were used in this study. Sixty percent of these patients received combination therapy of adalimumab together with immunomodulators. CR-70 was 68.7%, 74.5% and 88.4% after week 4, 8 and 12 of treatment, respectively. The steroid-free rate, complications and survival were 47.6%, 9.7% and 99% of patients, respectively. In considering the mucosal healing, only 25% patients achieve mucosal healing after treatment for 6 to12 months. Surgery was still needed in 16.5% of patients. Combination treatment of adalimumab with immunomodulators further decreased the level of CDAI at week 8 when compared with the monotherapy. Conclusions: Even under the stringent criteria for using adalimumab, the response rate was comparable to those without stringent criteria. (Intest Res 2014;12:287-292)

Analysis of dynamical stability of rigid-flexible hybrid-driven lower limb rehabilitation robot

Yan-lin Wang,Ke-yi Wang,Zi-xing Zhang,Zhuang Han,Wan-li Wang 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.4

The research of the safety problem of the cable-driven lower limb rehabilitation robots (CDLR) is not considered in the related report. Hence, the purpose of this paper is to study the dynamical stability evaluation method of a rigid-flexible hybrid-driven lower limb rehabilitation robot system (RFHDLR) to meet the training requirements of different patients in different rehabilitation stages. In order to improve the flexibility and operational performance of the robot system, one rigid motion chain is introduced into the cable-driven parallel robot (CDPR) to form a RFHDLR. Secondly, the motion planning strategy of the rigid motion chain is analyzed. Then, based on the kinematics and dynamics of the robot system, the cable tension performance factor and the system stiffness performance factor of the robot system are defined, and the static stability evaluation method and index of RFHDLR are obtained by the weighting method. Considering the patient's ability to withstand the motion velocity and the effect of the volatility of slide motion velocity of the rigid motion chain on the safety of the robot system, the velocity performance factor of the robot system is proposed. The dynamical stability evaluation method and index of RFHDLR are discussed based on the static stability evaluation method and the velocity influence function. Finally, for the two planning strategies of the rigid motion chain, the experimental study of the planned training trajectory of the lower limb traction point is performed based on simulation analysis. The experimental results verify the correctness of the stability evaluation method and provide a reference for further studying the training task planning and the control strategy.