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Changes in potassium concentration and gene expression in mice fed a high-fat diet
Junkoo Yi,Rijin Kang,Zaeyoung Ryoo,Duhak Yoon,Sunghyun Kim,Myoungok Kim 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.4
Obesity is a risk factor for various diseases, including cardiovascular disease, diabetes, renal disease, hypertension, cancer, and neural disease. Adipose tissue in animals is important for the mobilization of lipids, milk production, deposition of fat in different depots, and muscle and meat production. Understanding the genetic and physiological causes of metabolic disease is a priority in biomedical genome research. In this study, we examined several variables in mice fed a high-fat diet, including serum composition, body weight, total calorie intake, and differentially expressed genes. Body weight and blood glucose levels were not significantly different between animals fed high-fat and normal diets. However, high-fat diet groups showed reduced calorie and food intakes. Levels of sodium, ionized calcium, glucose, hematocrit, hemoglobin, pH, PCO2, PO2, TCO2+, HCO3+, base excess, and SO2 in the blood were not significantly different between mice fed high-fat and normal diets. Serum potassium concentration, however, was lower in mice a high-fat diet. Differentially expressed genes were also compared between the two groups. The purpose of this study was to discover new genes as a result of annealing control primer (ACP) PCR using 20 random primers. Five down regulated genes were identified and three of others were up-regulated by high-fat diet. Known genes were excluded from this result. In addition, the relationships among candidate genes and high-fat diet should be investigated according to potassium concentration in the blood. In conclusion, mice fed normal and high-fat diets showed no significant difference in body weight, whereas high-fat diet led to changesin blood composition and differential expression of several genes. These findings may provide a better understanding of the mechanisms underlying the association between obesity and metabolic diseases.
Change of Blood Composition and Expression Genes by the High Fat Diet in Mice
JunKoo Yi,Jaejung Ha,Dongyep Oh,Myoung Ok Kim 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
Obesity is a risk factor for various diseases, including cardiovascular disease, diabetes, renal disease, hypertension, cancer, and neural disease. Adipose tissue in animals is important for the mobilization of lipids, milk production, deposition of fat in different depots, and muscle and meat production. Understanding the genetic and physiological causes of metabolic disease is a priority in biomedical genome research. In this study, we examined several variables in mice fed a high-fat diet, including serum composition, body weight, total calorie intake, and differentially ex\-pressed genes. Body weight and blood glucose levels were not significantly different between animals fed high-fat and normal diets. However, high-fat diet groups showed reduced calorie and food intakes. Levels of sodium, ionized calcium, glucose, hematocrit, hemoglobin, pH, PCO2, PO2, TCO2+, HCO3+, base excess, and SO2 in the blood were not significantly different between mice fed high-fat and normal diets. Serum potassium concentration, however, was lower in mice a high-fat diet. Differentially expressed genes were also compared between the two groups. The purpose of this study was to discover new genes as a result of annealing control primer (ACP) PCR using 20 random primers. Five down regulated genes were identified and three of others were up-regulated by high-fat diet. Known genes were excluded from this result. In addition, the relationships among candidate genes and high-fat diet should be investigated according to potassium concentration in the blood. In conclusion, mice fed normal and high-fat diets showed no significant difference in body weight, whereas high-fat diet led to changes in blood composition and differential expression of several genes. These findings may provide a better understanding of the mechanisms underlying the association between obesity and metabolic diseases.
Effect of Egg Yolk Combination on Freezing thawed Semen Function in Korean Hanwoo Bull
JunKoo Yi,Jaejung Ha,Dongyep Oh,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Since the first report of the successful preservation of sperm, cryopreservation has been applied as a routine technique for processing bovine sperm in artificial insemination (AI), and numerous studies have been carried out to evaluate fundamental biological properties. Although fertility with frozen–thawed bull semen was generally acceptable for AI, the cryopreservation techniques at the time still resulted in the loss of 40 ~50% of viable sperm during the freezing–thawing process with little improvement over the last several decades. Cold shock, osmotic stress, ice crystal formation or oxidative damage were the main sources of sperm cryoinjury, and finally caused the loss of sperm viability and fertility. This study was designed to compare fresh and insemination egg yolk in an extender for cryopreservation of Hanwoo bull semen. Sperm motility, plasma membrane integrity and viability were assessed at different stages of cryopreservation (post-dilution, pre-freezing and post-thawing). Sperm plasma membrane integrity remained similar at all the stages of cryopreservation. Sperm motility and viability were significantly higher after thawing in the extender containing insemination egg yolk. In conclusion, insemination egg yolk may be used in an extender for the cryopreservation of Hanwoo bull spermatozoa.
Junkoo Yi,Jinyeon Park,Jaejung Ha,Daejung Yu,Woo-Sung Kwon,Daehyun Kim 한국동물생명공학회(구 한국수정란이식학회) 2023 한국동물생명공학회지 Vol.38 No.4
Background: Estrus in cows can be detected through vaginal electrical resistance or conductivity. However, there are no studies measuring vaginal electrical resistance in Hanwoo cows. This study aims to measure the vaginal electrical resistance value in Hanwoo cows and compare it with estrus and ovulation. Methods: Vaginal electrical resistance values of 73 Hanwoo cows were measured before and after estrus at the Gyeongsangbuk-do Livestock Research Institute. Measurements were taken on days -6, -3, -2, -1, 0, 1, 2, 3, and 6 of artificial insemination. Large follicles and ovulation were confirmed using transvaginal ultrasonography. Results: The vaginal electrical resistance averaged 225.6 ± 6.3 Ω days before the artificial insemination date, decreasing until the day of artificial insemination. The average vaginal electrical resistance was 163.7 ± 4.6 Ω on the date of artificial insemination, and 188.8 ± 4.3 Ω one day after artificial insemination, when large follicles were observed. In addition, on the 6th day after artificial insemination, the vaginal electrical resistance averaged 231.4 ± 5.5, which was similar to the 6th day before artificial insemination (222.5 ± 6.3). Transvaginal ultrasonography showed that most of the cows ovulated one day after artificial insemination. Conclusions: The accuracy of estrus is high if the vaginal electrical resistance is measured for cows with confirmed estrus, making is a potentially useful for determining the timing of artificial insemination.
Haibo Zhang,Junkoo Yi,윤두학,류재웅,In Kyu Lee,김명옥 대한암예방학회 2020 Journal of cancer prevention Vol.25 No.4
Hepatocellular carcinoma (HCC) is the most common primary liver cancer and is one of the leading causes of cancer-related deaths worldwide. Imatinib and GNF-5 are breakpoint cluster region-Abelson murine leukemia tyrosine kinase inhibitors which have been approved for the treatment of chronic myeloid leukemia and various solid tumors. However, the effect and underlying mechanisms of imatinib and GNF-5 in HCC remain poorly defined. In this study, we investigated the anticancer activity and underlying mechanisms of imatinib and GNF-5 in HepG2 human hepatocarcinoma cells. Cell proliferation and anchorage-independent colony formation assays were done to evaluate the effects of imatinib and GNF-5 on the growth of HepG2 cells. The cell cycle was assessed by flow cytometry and verified by immunoblot analysis. Gene overexpression and knockdown assays were conducted to evaluate the function of S-phase kinase-associated protein 2 (Skp2). Imatinib and GNF-5 significantly inhibited the growth of HepG2 cells. Imatinib and GNF-5 induced G0/G1 phase cell cycle arrest by downregulating Skp2 and upregulating p27 and p21. Overexpression of Skp2 reduced the effect of imatinib and GNF-5 on HepG2 cells. Knockdown of Skp2 suppressed the proliferation and induced G0/ G1 phase arrest. Furthermore, knockdown of Skp2 enhanced the effect of imatinib and GNF-5 on growth of HepG2 cells. In conclusion, imatinib and GNF-5 effectively suppress HepG2 cell growth by inhibiting Skp2 expression. Skp2 promotes the cell proliferation and reverse G0/G1 phase cell cycle arrest and it represents a potential therapeutic target for HCC treatment. Key Words Hepatocellular carcinoma, Cell cycle, S-phase kinase-associated protein 2, Imatinib, GNF-5
Haibo Zhang,Junkoo Yi,휘앙하이,Si Jun Park,권욱봉,김은경,So-Young Jang,Si-Yong Kim,최성균,Du-Hak Yoon,Sung-Hyun Kim,Kangdong Liu,Zigang Dong,Zae Young Ryoo,Myoung Ok Kim 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.3
Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenosideRh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in manycancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 inCRC cells in vitro and in vivo. Methods: Cell proliferation, migration, invasion, apoptosis, cell cycle, and western blot assays wereperformed to evaluate the effect of G-Rh2 on CRC cells. In vitro pull-down assay was used to verify theinteraction between G-Rh2 and Axl. Transfection and infection experiments were used to explore thefunction of Axl in CRC cells. CRC xenograft models were used to further investigate the effect of Axlknockdown and G-Rh2 on tumor growth in vivo. Results: G-Rh2 significantly inhibited proliferation, migration, and invasion, and induced apoptosis andG0/G1 phase cell cycle arrest in CRC cell lines. G-Rh2 directly binds to Axl and inhibits the Axl signalingpathway in CRC cells. Knockdown of Axl suppressed the growth, migration and invasion ability of CRCcells in vitro and xenograft tumor growth in vivo, whereas overexpression of Axl promoted the growth,migration, and invasion ability of CRC cells. Moreover, G-Rh2 significantly suppressed CRC xenografttumor growth by inhibiting Axl signaling with no obvious toxicity to nude mice. Conclusion: Our results indicate that G-Rh2 exerts anticancer activity in vitro and in vivo by suppressingthe Axl signaling pathway. G-Rh2 is a promising candidate for CRC prevention and treatment.