부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

A multi-functional polyhydroxybutyrate nanoparticle for theranostic applications

Kwon, Hee-Su,Jung, Sung-Geun,Kim, Hae-Yeong,Parker, Stephanie A.,Batt, Carl A.,Kim, Young-Rok The Royal Society of Chemistry 2014 Journal of Materials Chemistry B Vol.2 No.25

<P>Biopolymer-based multi-functional nanoparticles have been developed through a one-step enzymatic polymerization reaction using engineered polyhydroxyalkanoate (PHA) synthase fused with green fluorescent protein (GFP) and a single chain variable fragment antibody (A33scFv) specific to colon cancer. PHA synthase possesses unique catalytic characteristics, namely covalent catalysis, by which the synthesized polyhydroxybutyrate chain remains covalently attached to the enzyme. The amphiphilic nature of the resulting protein-polymer hybrid gives rise to spontaneous self-assembly into a micellar structure with GFP and A33scFv displayed on the surface (AGPHB nanoparticle). A model compound, Nile red, was loaded into the hydrophobic core of the AGPHB nanoparticle during the polymerization and self-assembly process. The specificity of the fluorescent multi-functional AGPHB nanoparticle towards the colon cancer cell lines SW1222 (A33+) and HT29 (A33−) was confirmed and analysed quantitatively <I>in vitro</I>. This new biological approach provides a simple means of producing nanocarriers with a range of surface functionality and the sizes desired for imaging and targeted drug delivery.</P>

UPLC-PDA를 이용한 창포류의 분류 및 함량 분석

조지은(Ji Eun Jo),이아영(A Yeong Lee),김효선(Hyo Seon Kim),문병철(Byeong Cheol Moon),지윤의(Yunui Ji),천진미(Jin Mi Chun),김호경(Ho Kyoung Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3

A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites: β-asarone and α-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of β-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of α-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that β-asarone and α-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.

Network topology and resilience analysis of South Korean power grid

Kim, Dong Hwan,Eisenberg, Daniel A.,Chun, Yeong Han,Park, Jeryang Elsevier 2017 PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIO Vol.465 No.-

<P><B>Abstract</B></P> <P>In this work, we present topological and resilience analyses of the South Korean power grid (KPG) with a broad voltage level. While topological analysis of KPG only with high-voltage infrastructure shows an exponential degree distribution, providing another empirical evidence of power grid topology, the inclusion of low voltage components generates a distribution with a larger variance and a smaller average degree. This result suggests that the topology of a power grid may converge to a highly skewed degree distribution if more low-voltage data is considered. Moreover, when compared to ER random and BA scale-free networks, the KPG has a lower efficiency and a higher clustering coefficient, implying that highly clustered structure does not necessarily guarantee a functional efficiency of a network. Error and attack tolerance analysis, evaluated with efficiency, indicate that the KPG is more vulnerable to random or degree-based attacks than betweenness-based intentional attack. Cascading failure analysis with recovery mechanism demonstrates that resilience of the network depends on both tolerance capacity and recovery initiation time. Also, when the two factors are fixed, the KPG is most vulnerable among the three networks. Based on our analysis, we propose that the topology of power grids should be designed so the loads are homogeneously distributed, or functional hubs and their neighbors have high tolerance capacity to enhance resilience.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Detailed information on power grid may produce highly-skewed degree distribution. </LI> <LI> Resilience of KPG is analyzed with multiple approaches including recovery. </LI> <LI> KPG is revealed as most vulnerable compared to ER and BA networks. </LI> </UL> </P>

Liquid System을 이용한 적혈구계 세포 배양의 정량적 분석

한진영,김태겸,김경희,김아성,배혜란,서덕준,강설미,박주인,김인후 大韓血液學會 1997 大韓血液學會誌 Vol.32 No.1

The association between prion proteins and Aβ1-42 oligomers in cytotoxicity and apoptosis

Hyeon, Jae Wook,Kim, Su Yeon,Park, Jun Sun,Choi, Bo Yeong,Lee, Sol Moe,Ju, Young Ran,An, Seong Soo A.,Kim, Chi-Kyeong Elsevier 2012 Biochemical and biophysical research communication Vol.424 No.2

<P>Misfolding of prion protein (PrP to PrPSc) can cause neurodegenerative prion diseases. As a glycosylphosphatidylinositol (GPI)-anchored membrane protein, the normal form of PrP (PrPC) can function as a receptor for ligands in the extracellular space. PrPC was suggested to be involved in memory, synaptic neuronal communication, and anti-oxidation as a neuroprotective agent. The recently identified interaction between PrPC and Aβ(1-42) oligomers suggested another role for PrP as a receptor for Aβ(1-42) oligomers, thereby influencing cytotoxicity and apoptosis. Here, the association between PrPC and Aβ(1-42) oligomers was investigated by visualizing protein localization in neuronal cells by immunocytochemistry. Aβ(1-42) oligomer-induced cytotoxicity was tested in respective expressions of PrPC by using mouse neuroblastoma-2a (N2a) cells, the prion protein overexpressed cells (L2-2B1), and a Prnp-null mouse hippocampal cell line (HpL 3-4). Moreover, apoptotic proteins such as caspase-8 were used to assess the effect of PrPC on Aβ(1-42) oligomer-mediated apoptosis. In L2-2B1 and HpL 3-4 cells, the difference in the cytotoxicity of Aβ(1-42) oligomers could be clearly distinguished. In addition, Aβ(1-42) oligomers induced mitochondria dysfunction, reactive oxygen species (ROS) generation, and calcium influx PrPC-dependently. Apoptosis, related to mitochondria dysfunction, was further investigated to determine the cytotoxic pathway; the results suggest that PrPC could be involved in both the intrinsic and extrinsic apoptotic pathways. Finally, cells with abundant PrPC expression seemed to be more susceptible to Aβ(1-42) oligomer toxicity, suggesting the importance of the level of PrPC expression in the induction of apoptosis.</P>

Effects of Aralia continentalis and Angelica biserrata on Inflammatory Response in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Phorbol Ester-induced Ear Edema

Myeong Sook Cheon,윤태숙,Seung Ju Kim,Goya Choi,문병철,A-Yeong Lee,Byung Kil Choo,Ho Kyoung Kim,So Yeon Yu,Ken Yasukawa 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.2

The roots of both Aralia continentalis and Angelica biserrata, known as ‘Dokwhal’ in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin E2, interlukin-1beta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.

청동풍탱이(Anomala albopilosa)추출물의 항산화성 및 생리기능

윤원종,이정아,김지영,김상범,박수영,Yoon, Weon-Jong,Lee, Jung-A,Kim, Ji-Young,Kim, Sang-Bum,Park, Soo-Yeong 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.6

산화적인 스트레스(oxidative stress)는 신경염증의 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충으로 알려진 청동풍뎅이(Anomala albopilosa) 성충과 3령 유충(궁벵이) 에탄올 추출물을 대상으로 항산화활성을 비교 측정하였으며, 또한 대식세포인 RAW 264.7 세포에서의 NO 의 생성억제 효과 및 세포독성 그리고 분자 염증관련 인자인 iNOS, COX-2와 $PGE_2$ 생성 및 활성 억제를 조사하여 항염 증효과 등의 생리활성을 측정 비교하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 향산화 실험과 관련하여 청동풍탱이 성충과 3령 유충 에탄올 추출물 의 DPPH(1,1-diphenyl-2-picrylhydrazyl) radical 소거능, superoxide radical 소거능, xanthine oxidase 저해활성 그리고 nitric oxide 소거능에 대한 항산화활성 등의 assay를 실시하였다. 그 결과, 청동풍뎅이 에탄올 추출물은 항산화능을 갖고 있었으며, 3령 유충 추출물에서보다는 성충 추출물에서 다소 높은 항산화활성을 보여주었다. 또한 RAW 264.7 세포에 LPS로 자극을 주고 청동풍뎅이 성충과 3령 유충 에탄올 추출물을 처리하여 항염증관련 활성을 확인해본 결과, 고농도에서는 다소 세포독성을 나타냈지만 농도의존적으로 NO, iNOS, COX-2 그리고 $PGE_2$의 생성 억제효과가 나타났다. 이러한 결과는 유용곤충자원을 이용한 유효성분 추출을 통한 항산화 및 항염증 물질의 연구 또는 예방하거나 치료할수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 사료된다. We analyzed antioxidant and physiological activities to investigate the functional effects of ethanol extracts of Anomala albopilosa imago and A. albopilosa larva. In order to effectively screen for anti-inflammatory agents, we first investigated the antioxidant activities such as DPPH radical scavenging capacity, superoxide radical scavenging capacity, xanthine oxidase inhibitory activity, and nitric oxide scavenging capacity of the A. albopilosa extracts. By the screening system, we found that A. albopilosa extracts had antioxidant activity which increased with increments of the extract concentration. Moreover, we examined the inhibitory effect of the A. albopilosa extracts on the production of anti-inflammatory factors that the nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and prostaglandin $E_2\;(PGE_2)$ production activated with LPS ($1{\mu}g/mL$) in murine macrophage cell line RAW 264.7. A. albopilosa extracts potentially inhibited the iNOS and COX-2 in a dose-dependent manner. The inhibition of iNOS activity was correlated with the decrease in nitrite levels. Additionally, the $PEG_2$ production is markedly inhibited after a treatment with the A. albopilosa extracts.

Effects of Aralia continentalis and Angelica biserrata on Inflammatory Response in Lipopolysaccharide-Induced RAW 264.7 Macrophages and Phorbol Ester-induced Ear Edema

Cheon, Myeong-Sook,Yoon, Tae-Sook,Yasukawa, Ken,Yu, So-Yeon,Kim, Seung-Ju,Choi, Go-Ya,Moon, Byeong-Cheol,Lee, A-Yeong,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.2

The roots of both Aralia continentalis and Angelica biserrata, known as 'Dokwhal' in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin $E_2$, interlukin-lbeta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.

저염 우렁쉥이 젓갈의 가공 및 갈변방지

김영아,강수태,강군중,오광수 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.4

These studies were carried out to develop the low salt-fermented sea foods from ascidian. In present papers, we examined optimum processing conditions and quality improvement of the low salt-fermented ascidian. The optimum processing conditions of low salt-fermented ascidian was as followed. After deshelling and slicing a ascidian muscle in 5 mm width, and dipping in a 10% salt and 1% sodium erythorbate solution for 20 min. We dewatered it, and dipped it in 0.1% sodium bisulfite solution for 1 min, then added an anchovy sauce and rice gruel(1:1) mixture and finally fermented it at 5℃ for 15 days. The moisture and salinity contents of the low salt-fermented ascidian were 75.0~75.4% and 8.0 ~8.5%, respectively. During fermention at 5℃ for 20 days, amino-N contents of the low salt-fermented ascidian increased, and texture were softened gradually. The viable cell counts were 4.2~4.5×10^(4) CFU/g, and tended to decrease in the first stage during fermentation. The ratio of saturated fatty acids tended to increase in the early stage of fermentation, while that of polyunsaturated fatty acids decreased slightly during fermentation. In the results of chemical experiments and sensory evaluation, dipping treatment in 1% sodium erythorbate solution and 0.1% sodium bisulfite solution showed a good color and prevention of browning in the low salt-fermented ascidian, and also adding anchovy sauce and rice gruel mixture improved the taste of it.