발바닥 특정 부위 자극이 뇌파에 미치는 효과에 대한 비선형 분석

吳永仙,吳旼錫,宋泰元 대전대학교 한의학연구소 2001 한의학연구소 논문집 Vol.10 No.1

The brain is one of the most complex systems in nature. Brain waves, or the "EEG", are electrical signals that can be recorded from the brain, either directly or throught the scalp. The kind of brain wave recorded depends on the behavior of the animal, and is the visible evidence of the kind of neuronal (brain cell) processing necessary for that behavior. But, EEG had been considered as a virtually infinite-dimensional random signal. However, nonlinear dynamics light on dynamical aspects of the human EEG. The methods of nonlinear dynamics provide excellent tolls for the study of multi-variable, complex system such as EEG. In this study, 20 persons seperated in 2 groups were examined with EGG, one group stimulated on specific area of the sole of the foot with footbed inside the shoes. This experiment resulted in at the group stimulated on specific area of the sole of the fott correlation dimension of P4 and Ol channels increased significantly. Therefore, we obserbed that stimulation on specific area of the body had a constant effections on the specific channels.

BellaGel breast implant: 6-Year results of a prospective cohort study

Oh, Joon Seok,Jeong, Jae Hoon,Myung, Yujin,Oh, Jeongseok,Kang, Shin Hyeok,Park, Eonju,Kim, Ara,Bang, Sa Ik,Heo, Chan Yeong Korean Society of Plastic and Reconstructive Surge 2020 Archives of Plastic Surgery Vol.47 No.3

Background This is the first clinical study conducted among Asian women using breast implants manufactured by an Asian company. Four-year data regarding the safety and efficacy of BellaGel breast implants have already been published, and we now report 6-year data. Methods This study was designed to take place over 10 years. It included 103 patients who underwent breast reconstruction or augmentation using BellaGel breast implants. The rates of implant rupture and capsular contracture were measured and analyzed to evaluate the effectiveness of the breast implant. Results At patients' 6-year postoperative visits, the implant rupture and capsular contracture rates were 1.15% and 2.30%, respectively. The implant rupture rate was 3.77% among reconstruction cases and 0% among augmentation cases. The capsular contracture rate was 5.66% among reconstruction cases and 0.83% among augmentation cases. Conclusions The 6-year data from this planned 10-year study suggest that the BellaGel cohesive silicone gel-filled breast implant is an effective and safe medical device that can be used in breast reconstruction and augmentation.

Effects of Leydig cell-specific Esr1 deletion on male reproduction in mice

Yeong Seok Oh,Myung Chan Gye 한국발생생물학회 2015 한국발생생물학회 학술발표대회 Vol.2015 No.9

Estrogen plays an important role both in male and female reproduction. Two estrogen receptor isoforms, Esr1 and Esr2, are expressed in male gonad. In the mouse, Esr1 is expressed in Leydig cells of testis and pituitary. Esr1-/- male mice show enhanced androgen synthesis, spermatogenic defect, and infertility. To evaluate the specific function of Esr1 in Leydig cells, we examined spermatogenesis and steroidogenesis in Esr1f/fCyp17iCre male mice in which Esr1 is deleted specifically in Leydig cells. These mice showed normal spermatogenesis and fertility when compared to wild type from young adulthood to old age. Testosterone synthesis in Esr1f/fCyp17iCre mice at 3-12 months old of age was not different from age-matched wild type mice, while, at 18 months old of age, circulating testosterone concentrations were significantly higher than wild type together with increased levels of Star, Cyp17a1, and Hsd17b3 mRNA and with a hypertropy of Leydig cells. In Esr1f/fCyp17iCre mouse pituitaries, Fshb and Lhb mRNA levels were not different from wild type from young adulthood to old age. Taken together, Esr1 in Leydig cells may be not essential for spermatogenesis and fertility under control of endogenous estrogens and may have a role in aged Leydig cell function.

Regulation of estrogen sulfotransferase expression by estrogen receptor alpha in mouse testis

Yeong Seok Oh,Seung Seok Ok,Myung Chan Gye 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8

Estrogen sulfotransferase (EST) is a cytosolic enzyme that catalyzes the sulfo-conjugation of estrogens at the 3-hydroxyl position. Sulfated estrogens lose their ability to interact with the estrogen receptor (ER). Previous studies have reported that testicular expression of EST is under the regulation of LH and androgen. In an effort to understand the biological significance of estrogens in the testis, we analyzed the EST gene expression in the developing mouse testis and Leydig cells and its regulation by estrogen receptor alpha (ERα). Male mice at postnatal day (PND) 1, 7, 14, 28, and 56 and ERα flox/flox Cyp17iCre male mice which show deletion of ERα specifically in Leydig cells were used for this study. Testes and Leydig cells isolated from these mice were subjected to quantitative RT-PCR analysis and immunohistochemistry. In addition, 17β-estradiol (E2), ERα-selective agonist PPT, ERβ -selective agonist DPN, and ER antagonist ICI 182,780 were treated in primary adult Leydig cell culture. These cultured cells were subjected to quantitative RT-PCR analysis. In testis, EST mRNA level was excessively low by PND 14 and markedly increased from puberty (PND 28) onward. In the interstitium, EST mRNA was not detected by PND 14 but considerably expressed from PND 28 onward. EST immunoreactivity was moderate in the interstitium by PND 14. Strong EST immunoreactivity was found in the interstitium from PND 28 onward. In ERαflox/flox Cyp17iCre mouse testis and Leydig cells, EST mRNA level was significantly lower than wild type (ERαflox/flox). In primary adult Leydig cell culture, the expression of EST mRNA was increased by E2 and PPT, but was not changed by DPN. The expression of EST in the testis is developmentally regulated. In adult Leydig cells, EST could play an important role in the steroidogenesis by modulating the activity of estrogens. Estrogen as well as LH and androgen may play a role in the regulation of EST expression in Leydig cells via ERα signaling.

Estrogen receptor alpha is differentially expressed in Leydig cells during postnatal development and negatively regulates steroidogenesis and proliferation of progenitor Leydig cells in mouse testis

Yeong Seok Oh,Myung Chan Gye 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9

Estrogen is an important regulator of reproduction in both male and female. The two forms of estrogen receptor (ER) are known, ERα and ERβ. To understand the role of ERα in the testis, we investigated the expression of ERα in the mouse Leydig cells during postnatal development and the effects of estrogen on steroidogenesis and proliferation in progenitor Leydig cells (PLCs). In the testis, ERα mRNA and protein levels were markedly increased from postnatal day (PND) 1 to 14 and decreased thereafter until PND 56. During postnatal development ERα immunoreactivity was strong in the nucleus of Leydig cells at PND 14 when PLCs were abundant in the interstitium and low in the mature adult Leydig cells (ALCs). In fetal Leydig cells (FLCs), ERα immunoreactivity was negligible at birth and became increased at PND 14. This suggests an important role of ERα in Leydig cells during neonatal period. In isolated PLCs, 17β-estradiol (E2) and ERα-selective agonist, PPT suppressed the hCG-induced progesterone production and steroidogenic pathway genes expression. The hCG-induced PLCs proliferation was significantly inhibited by E2 and PPT. In conclusion, estrogen - ERα signaling may negatively regulate functional differentiation and proliferation of PLCs.

Fucosyl neoglycoprotein binds to mouse epididymal spermatozoa and inhibits sperm binding to the egg zona pellucida

Yeong Seok Oh,Myung Chan Gye 한국발생생물학회 2012 한국발생생물학회 학술발표대회 Vol.31 No.-

Glycan epitopes of cellular glycoconjugates act as versatile biochemical signals, and this sugar coding plays an important role in cell-to-cell recognition processes. In the present study, our aims were to determine the distribution of sperm receptors with activity for fucosyl- and galactosyl glycans and to address whether mono sugar neoglycoproteins functionally mimic the binding between zona pellucida (ZP) glycoproteins and sperm. In mouse epididymal spermatozoa with intact acrosomes, fucopyranosyl bovine serum albumin (BSA-Fuc) bound to the segment of the acrosome, the equatorial segment, and the postacrosome region of the sperm head. Galactosyl BSA (BSA-Gal) binding activity was similar to that of BSA-Fuc, but was weaker. In acrosome-reacted spermatozoa treated with the Ca2+ ionophore A23187, BSA-Fuc binding was lost in the apical segment of the acrosome but remained in the equatorial segment and postacrosome regions. BSA-Gal binding to the equatorial region was increased. In the presence of 2.5 μg/ml BSA-Fuc, in vitro sperm - ZP binding was significantly decreased, indicating that fucosyl-BSA functionally mimics ZP glycoproteins during sperm-egg ZP interactions. At the same concentration, BSA-Gal was not effective. Fucosyl BSA which efficiently inhibited the sperm-ZP binding can mimic the ZP glycoconjugate and has potential for use as a sperm fertility control agent in mouse.

Aquaporin 5 is regulated by estrogen and LH in mouse Leydig cells and is involved in steroidogenesis

Yeong Seok Oh,Myung Chan Gye 한국발생생물학회 2014 한국발생생물학회 학술발표대회 Vol.2014 No.9

Water channel proteins, aquaporins (AQPs) contribute to transepithelial water movement in many tissues. To date, 13 mammalian AQPs have been identified. Of these, AQP5 plays an important role in the fluid homeostasis and cell volume control in epithelial cells. In an effort to understand the role of AQP5 in testis, we investigated the expression of AQP5 in developing mouse testis, its regulation by estrogen and LH, and the change of steroidogenesis by AQP5 knockdown. Testes and Leydig cells were isolated from male mice at postnatal day (PND) 1, 7, 14, 28, and 56 and estrogen receptor alpha knockout (ERαKO) mice. In mouse testis, AQP5 immunoreactivity was negligible by PND 14. From PND 28 onward, AQP5 immunoreactivity was found in Leydig cells. In ERαKO mouse Leydig cells, AQP5 mRNA level was significantly lower than wild type. In primary adult Leydig cell culture, the expression of AQP5 mRNA was increased by 17β-estradiol (E2) and human chorionic gonadotropin (hCG), but was not changed in ERαKO Leydig cells. Moreover, the expression of AQP5 mRNA was increased by E2 and ERα-selective agonist PPT, but was not changed by ERβ-selective agonist DPN in primary Leydig cells and mLTC-1. In silico analysis and chromatin immunoprecipitation (ChIP) assay revealed that there are putative estrogen response elements (EREs) and cAMP response elements (CRE) in AQP5 promoter region. Testosterone secretion and steroidogenic pathway genes (StAR, Cyp11a1, Cyp17a1, and 3β-HSD6) expression were decreased by AQP5 siRNA in primary Leydig cells. In conclusion, AQP5 expression was coupled with functional differentiation of adult Leydig cells. AQP5 may play an important role in the fluid homeostasis and cell volume control during development of adult Leydig cells. The expression of AQP5 in Leydig cells could be regulated by ERα and LH signaling and AQP5 may be involved in steroidogenesis.

작업장 보안 및 운영 효율성 향상을 위한 OPC UA 기반 최적화 연구

오영석(Yeong-Seok OH),송병훈 대한산업공학회 2024 대한산업공학회 춘계학술대회논문집 Vol.2024 No.5

본 연구는 스마트 팩토리 환경에서 객체 인식 기술과 OPC UA의 통합을 탐구한다. 효율성과 안전성 향상을 위해 중요한 역할을 한다. 이를 위해 OPC UA 서버는 이기종 기기 간 통신을 원활하게 하고 잠재적 위험에 대한 실시간 감지 및 대응을 가능하게 한다. 특히, 객체 검출 딥러닝 기술을 활용한 지능형 감시 시스템 개발과 인간-로봇 상호작용의 안전성 향상을 통해 작업자의 안전을 강화하고, 로봇 간의 효율적인 공간 활용 방법을 제안한다.

지상 이동체의 모델 기반 고장진단 알고리즘

오석영(Seok Yeong Oh),김용훈(Yong Hun Kim),고보성(Bo Sung Ko),정재훈(Jae Hun Jung),이주현(Joo Hyun Lee),송진우(Jin Woo Song) 대한전기학회 2024 대한전기학회 학술대회 논문집 Vol.2024 No.7

Effect of High Hydrostatic Pressure for Shelf-life and Chemical Characteristics of Green Yellow Juices

Nam Seok Oh,Myoung Sub Song,Min Young Kim,Gwi Yeong Jang,Yoon Jeong Lee,Meishan Li,Yeong Mi Ji,Eun Hee Kim,Heon Sang Jeong 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10