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소의 단위 발생란과 체외수정란 유래의 할구 응집에 의한 키메라 수정란(Chimeric Embryo)의 발달
E. H. Yeao,Kim, Y. S.,Lee, S. L.,T. Y. Kang,D. O. Kwack,Lee, H. J.,S. Y. Choe 韓國受精卵移植學會 2003 한국동물생명공학회지 Vol.18 No.1
발생학에서 키메라(chimera)는 2개 이상의 다른 유전자형의 세포, 또는 다른 종의 세포로부터 만들어진 1개의 생물개체를 뜻하는 말로, 이는 초기 수정란의 발달과 포유류의 분화를 연구하는데 이용되고 있다. 키메라를 만드는 방법에는 할구와 내세포괴를 응집시키는 방법과 배반포 내에 여러 종류의 세포를 주입하는 방법이 있다 본 실험에서는 서로 다른 두 가지 방법의 활성화 처리법, 즉, ionomycin 처리 후 Cycloheximide (CHX) 또는 6
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김순례,이경왜,Kim, Soon-Lae,Lee, Kyeong-Yeao 한국가정간호학회 1999 가정간호학회지 Vol.6 No.-
In oder to investigate the basic data for the standard of visiting nursing activities and educational program for Public Health Nurses in Health Center in Korea. the health record review carried out regard to visiting nursing services of 131 subjects with visiting nursing care. The results were as follows: 1. Rates of visiting nursing services offered by Public Health Nurse of Health Center(in decreasing order)were health education and disease management(98.5%), activity assess-ment(94.7%), counseling(90.8%), dietary care (61.1%), exercise treatment(60.3%), examination(58.8%), medication(48.9%), referral to hospital(32.8%, vaccination against flu and hepatitis(29.8%), support for welfare and administration(24.4%), position change(9.9%), connection to voluntary services(9.9%), wound dressing(7.6%) and referral for home health care nursing(3.1%). 2. The subjects consisted of 38.2% in male and 61.8% in female. 77.1% of the subjects had no job.
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Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)
Ock, S.A.,Kwack, D.O.,Cho, S.R.,Cho, S.K.,Yeao, E.H.,Yoo, J.G.,Lee, Y.R.,Lee, H.J.,Choe, S.Y.,Rho, G.J. 韓國受精卵移植學會 2002 한국동물생명공학회지 Vol.17 No.1
Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.
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