Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

절연재에 따른 TEM 안테나의 방사패턴 및 이득 변화

이예나(Ye-Na Lee),최순호(Sun-Ho Choi),허창수(Chang-Su Huh),최진수(Jin-Su Choi),박우철(Woo-Chul Park) 대한전기학회 2014 전기학회논문지 Vol.63 No.5

TEM Horn antenna is typically used in HPEM area. It is necessary to apply a high voltage of the antenna for radiating HPEM. Then, it is necessary to insulate essentially of applying a high voltage to the antenna. At this time, radiation pattern and gain of the TEM Antenna is changed. For this reason, it is necessary to analyze changing characteristics by using simulation. In this paper, I analyzed the radiation pattern of the antenna in accordance with the lens and insulating material. As a result, it was observed that the value of the gain is changed in accordance with the frequency. The lens is used for the antenna, the gain characteristic is improved.

취장 외분기능에 대한 체내 Amines의 역할

곽정재,김예식,명창선,김경환 연세대학교 의과대학 1987 연세의대논문집 Vol.20 No.2

Current Ripple Reduction Technique of DC/DC Converter

Ye-Then Chang,Chia-An Yeh,Masahiro Hamaogi,Fumikazu Takahashi,Yen-Shin Lai 전력전자학회 2007 ICPE(ISPE)논문집 Vol.- No.-

This paper presents a novel method to reduce the current ripple caused by the variation of inductor for a DC/DC converter. The presented method is to increase the switching frequency to keep constant current ripple when the inductance changes. Therefore, no extra component is required. The problem will be described first and the related method is presented. An experimental DC/DC converter is implemented which is with input voltage of 12 V, output voltage of 1.5 V, output current of 10 A, and switching frequency of 200-250 ㎑. Finally, experimental results derived from the designed digital-controlled DC/DC converter are included for confirmation.

담배 박각시나방 Manduca sexta의 계획된 세포죽음동안의 Proteasome의 변화

이구봉,우기민,조만희,이상한,장예진,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

모든 진핵세포의 주요 단백질 분해요소인 proteasome은 변성되거나 상해된 불필요한 단백질을 제거할 뿐만 아니라, 세포주기를 비롯한 다양한 대사과정들의 주요 효소들을 필요 시 적절히 분해함으로써 항상성을 유지시키는 필수적인 효소로 잘 알려져 있다. 또한 proteasome은 특정 포유동물 조직의 계획된 세포죽음(programmed cell death 또는 apoptosis)와 밀접하게 관련되어 있다고 보고된 바, 본 연구에서는 담배 박각시나방(Manduca sexta)의 변태과정에서 일어나는 마디간 근육(intersegmental muscle)의 급격한 파괴과정동안의 proteasome의 변화양상을 발현 및 단백질 분해활성을 중점으로 조사하였다. 정상근육과 파괴직전의 근육으로부터 proteasome을 분석한 결과 단백질 분해활성이 최소한 6배 이상 증가하였으며, proteasome의 주요 구성성분들의 발현 변화를 각각의 항체(antibody)를 이용하여 조사한 결과 세포죽음 직전에 급격히 증가함을 보였다. 이러한 구성성분들의 발현증가가 proteasome 복합체 형성을 통해 활성의 증가를 일으키는지 조사한 결과, 세포내에서 실질적으로 단백질 분해활성을 담당한다고 여겨지는 26S proteasome(2,000 kDa)의 위치에서 커다란 활성의 변화와 구성성분의 증가를 볼 수 있었다. 따라서, 담배 박각시나방의 계획된 세포죽음 현상은 특정 호르몬의 신호에 의해 proteasome의 발현이 급격히 증가하여 26S proteasome의 복합체 형태로 세포 내 대부분의 단백질들을 분해함으로써 30시간이내 근육조직을 완전히 소멸시키는 것을 알 수 있었다. 한편, proteasome을 구성하는 특정 구성성분의 과다 발현이 세포의 단백질 분해현상에 어떠한 영향을 미치는 지 조사하기 위하여 쥐의 fibroblast cell에 26S proteasome의 ATPase 구성성분인 m56 유전자를 삽입한 후 세포의 추출물로부터 proteasome의 양과 활성의 변화를 관찰하였다. Proteasome을 초원심분리로 추출하여 관찰한 결과, 담배 박각시나방의 경우와는 달리 유전자 삽입 세포는 모든 실험에서 정상 세포와 전혀 차이를 보이지 않았다. 이 결과로부터 세포의 생명 유지에 필수적이고 매우 다양한 기능을 가진 proteasome은 40여개의 구성성분들의 발현이 매우 정교하게 조절되고 있으며, 특정 세포죽음 자극(apoptotic signal)이 왔을 때 일련의 구성 성분들의 발현과 26S proteasome 복합체 형성이 증가함으로써 그 기능을 수행하는 것을 알 수 있으며, 보다 자세한 연구는 비정상적 단백질 분해나 세포죽음을 동반하는 여러 질환들의 원인과 현상을 규명할 수 있으며, 또한 그 활성을 조절함으로써 궁극적인 치료의 방향도 제시 가능할 것으로 기대된다.

A comparison of the treatment outcome of oral tofacitinib with other conventional therapies in refractory alopecia totalis and universalis: A retrospective study

( Ye Eun Kim ),( Jung-won Shin ),( Chang-hun Huh ),( Min-woo Kim ),( Ji-su Lee ),( Ohsang Kwon ),( Soyun Cho ),( Hyun-sun Park ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Treatment of alopecia totalis and alopecia universalis is often challenging and unsatisfactory. Recently, Janus kinase inhibitor has shown promising results. Objectives: The aim of this study is to compare the efficacy and tolerability between oral tofacitinib and conventional modalities for treating refractory alopecia totalis/universalis. Methods: A total 74 patients (18, treated with tofacitinib; 26, treated with conventional oral treatment (steroid ± cyclosporine); 30, treated with diphenylcyclopropenone) were included and their medical records were reviewed retrospectively. Results: After 6 months, 44.4% of patients in the tofacitinib group, 37.5% in the conventional oral treatment group, and 11.1% in the diphenylcyclopropenone group achieved 50% SALT change. During the treatment, 10% of patients in the tofacitinib group, 73.1% in the conventional oral treatment group, and 10% in diphenylcyclopropenone group experienced adverse drug reactions. Conclusion: In conclusion, oral tofacitinib was more effective than diphenylcyclopropenone immunotherapy and more tolerable than conventional oral treatment after 6 months of treatment.

인삼을 이용한 가공방법 개발과 효능검증

예은주,배만종,김수정,박창호 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 인삼의 가공방법을 개발하고 제품을 다양화하기 위한 기초 자료를 확립하고자, 우리나라의 전통주인 약주로 증자한 후 홍삼의 일반성분과 ginsenoside의 함량을 분석하고 갈색도 및 탁도의 변화를 알아보았다. 또한 항암효과를 검증하였던바 그 결과를 보고하고자 한다. 1. 백삼과 홍삼, 증자 횟수별 인삼의 갈색 변화를 보기 위해서 각 인삼의 60% 에탄올추출물의 갈변의 측정 결과 갈변의 전구물질과 중간생성물 및 fufural, 갈색계의 색소, 탁도 모두 증자횟수가 증가 할수록 값이 증가하였다. 그리고 백삼, 홍삼 A1는 3가지 항목에서 모두 비슷한 경향을 보였으며, 상대적으로 A3에서 높은 값을 보였다. 2. HPLC상으로 total ginsenoside의 함량을 측정한 결과 백삼 28.20mg/g, 홍삼 421.10mg/g이고 A1~A9에서는 46.10~76.80mg/g으로 홍삼과 백삼보다 증자 횟수가 증가 할 수록 total ginsenosied 함량이 증가하였다. 3. HPLC상에서 분석한 증자 횟수별 인삼의 ginsenosides(G-Rb₁, -Rb₂, -Rc, -Rd, -Re, -Rf, -Rg₁, -Rg₂, -Rg₃)의 함량변화는 G-Rc, -Rd, -Re는 1회 증자한 A1에서 함량이 증가 하다가 증자 횟수가 증가 할수록 다시 함량이 감소하였으며, G-Rg₂, -Rg₃는 증자 횟수와 비례하여 함량이 증가하는 경향을 보였다. 4. 인체유래 간암세포(Hep3B)에서의 항암효과 검증실험에서는 물, 60%에탄올 추출물을 각각 1000ppm, 2500ppm, 5000ppm으로 처리 한 후 세포 증식 억제율을 확인하였다. 백삼은 모든 처리군에서 미미한 증식억제 효과가 있었으나, 홍삼은 60%에탄올 추출물 5000ppm에서 54.52%, A9에서는 96.28%의 현저한 세포증식억제 효과가 있었다. A9가 Hep3B에 대해서 백삼과 홍삼보다 세포 증식 억제율이 더 높은 것을 확인 할 수 있었다. 이상의 결과로 볼 때 약주를 이용해서 증자한 홍삼이 백삼이나 일반홍삼보다 총 사포닌, 진세노사이드의 함량이 증가하였고, 기능과 효능이 증강된 것으로 확인되어 우수함이 입증되었다. 또한, 홍삼정과, 절편정과 등 제품의 다양화와 품질개선에 기여할 것으로 기대된다. 향후 증자 방법, 압력, 온도, 및 약주 등의 제조 조건에 따른 지속적인 연구가 요청된다. This study takes place to establish basic data to develop various ginseng-processing methods and products. It analyzed components and quantity of ginsenoside in red ginseng after brewing with the traditional medicinal wine as well as the brownness and impurity level changes, and verified anti-oxidization and anti-cancer effects. The following is the founding of these analyses. To examine the changes in the brownness of white and red ginseng, premonitory materials of 60% ethanol extracts were examined As the result of these examinations, all of the values of examined matters premonitory materials, mid products, Fufural, coloring matters and impurity level were increased as the brewing continued. And the brownness level for white and red ginseng was similar in Al and the values were relatively high in A3. The total Ginsenoside on HPLC was 28.20㎎/g for white ginseng and 42.10㎎/g for red ginseng. The quantity of total Ginsenoside was increased as the brewing continued as 46.10∼76.80㎎/g was shown in A1∼A9. To see the quantity changes of Ginsenosides(G-Rbi, -Rte, -Re, -Rd, -Re, -Rf, -Rg₁, -Rg₂, -Rg₃) contained in ginseng on HPLC, the quantity of G-Rc, -Rd, -Re were increased in Al the first brewing and decreased as the brewing continued. The quantity of G-Rg2, -Rg3 was increased proportionate to the number of times for brewing. In an anti-cancer test in liver cancer cells(Hep3B) extracted from human body, each of water and 60% ethanol extracts were processed with 1000ppm, 2500ppm, and 5000ppm and checked the multiplication restrain rate. All of the processing groups in white ginseng have minimum multiplication restrain rates, but red ginseng has 54.52% at 60% ethanol extract 5000ppm. At A9, 96.28%) of cell multiplication restrain effects were shown. Comparing Hep3B to A9, white ginseng has higher restrain effects than red ginseng. As seen in the above results, the reinforced red ginseng using the medicinal wine has higher total saponin and ginsenosides, as well as with increased functions and effects leading the superiority. And it is possible to use Red Ginseng Jung Kwa; Jung Kwas with flower pattern imprinted, and expect to increase the opportunities to use the extracts. Later, when brewing the pressure, temperature, and medicinal wine can be examined to continuous studies.

Korean Red Ginseng protects dopaminergic neurons by suppressing the cleavage of p35 to p25 in a Parkinson’s disease mouse model

Ye Lee Jun,Chang Hwan Bae,김동수,구성태,김승태 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.2

Background: Ginseng is known to have antiapoptotic, anti-inflammatory, and antioxidant effects. Thepresent study investigated a possible role of Korean Red Ginseng (KRG) in suppressing dopaminergicneuronal cell death and the cleavage of p35 to p25 in the substantia nigra (SN) and striatum (ST) using a1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease mouse model. Methods: Ten-week-old male C57BL/6 mice were injected intraperitoneally with 30 mg/kg of MPTP at24-h intervals for 5 d, and then administered KRG (1 mg/kg, 10 mg/kg, or 100 mg/kg) once a day for 12consecutive days from the first injection. Pole tests were performed to assess the motor function of themice, dopaminergic neuronal survival in the SN and ST was evaluated using tyrosine hydroxylaseimmunohistochemistry,and the expressions of cyclin-dependent kinase 5 (Cdk5), p35, and p25 in the SNand ST were measured using Western blotting. Results: MPTP administration caused behavioral impairment, dopaminergic neuronal death, increasedCdk5 and p25 expression, and decreased p35 expression in the nigrostriatal system of mice, whereas KRGdose-dependently alleviated these MPTP-induced changes. Conclusion: These results indicate that KRG can inhibit MPTP-induced dopaminergic neuronal death andsuppress the cleavage of p35 to p25 in the SN and the ST, suggesting a possible role for KRG in thetreatment of Parkinson’s disease.

기업 윤리프로그램에서 반추가 조직구성원의 비윤리적 행동의 변화와 그 효과의 지속성에 미치는 영향

이지예(Lee, Ji-Ye),장영균(Chang, Young-Kyun),김상수(Kim, Sang-Soo) 한국경영교육학회 2020 경영교육연구 Vol.35 No.4

[연구목적] 많은 기업에서 다양한 기업 윤리프로그램을 실시하고 있지만 그 실효성에 관해서는 많은 의문점이 제기되어 왔다. 교육학 등에서 주로 조명을 받아온 반추(reflection)는 조직의 내 · 외부적 이슈들과 관련하여 올바른 의사결정을 위해 어떻게 행동해야 하는지에 대한 표준을 능동적으로 학습할 수 있는 중요한 과정으로 여겨지고 있다. [연구방법] 본 연구에서는 기업 윤리프로그램에 반추과정을 적용하여 그 효과성을 살펴보고자 하였다. 이를 위해 총 339명을 대상으로 3개의 집단으로 구분하여 실험연구를 수행하였다. 윤리교육을 실시한 후 그 내용에 대한 반추의 과정을 거치는 실험집단1, 윤리교육만을 실시하는 실험집단2, 그리고 윤리교육과 반추의 과정 모두 실시하지 않는 통제집단으로 구분하여 반추의 효과성을 분석하였다. 또한 앞서 참여한 응답자를 대상으로 3주 후 다시 한번 동일한 측정문항으로 조사를 실시하여 그 효과의 지속성을 분석하였다. [연구결과] 실험 결과 윤리교육을 받은 실험집단1과 2에서 통제집단에 비하여 비윤리적 행동경향이 낮게 나타났는데, 특히 반추의 과정을 거친 실험집단1은 그렇지 않은 실험집단2에 비하여 비윤리적 행동 경향이 추가로 더 감소한 것으로 나타났다. 그리고 시간이 지남에 따라 윤리교육으로 인한 효과가 차차 소멸되기는 하지만 반추의 과정을 거칠 경우 그 효과가 더 지속되는 것을 알 수 있었다. [연구의 시사점] 이러한 결과를 통해 윤리프로그램에서 반추의 역할을 조명함과 동시에 기업의 윤리프로그램 효과성 제고에 실무적으로도 기여할 수 있을 것으로 기대한다. [Purpose] Reflection, usually getting more research attention in education, is a necessary component of ethics training because it encourages employees to be more proactive in learning the norms that guide their decision-making regarding various issues. [Methodology] This study designed an experiment by embedding reflection in an ethics training program to examine the effectiveness of such training. A total of 339 participants were allocated to two experimental groups (Group 1 and Group 2) and one control group. [Findings] Findings from the experiment showed that Group 1 and Group 2 are less inclined toward unethical behavior than the control group. Between Group 1 and Group 2, Group 1 that went through the reflection phase shows much less inclination toward unethical behavior than Group 2. The results further indicated that the impact of ethics training gradually diminished over time, but having reflection was found to help sustain the training impact. [Implications] Along with its theoretical contribution that sheds light on the role of reflection in ethics training programs, this study may be worthwhile by providing companies with practical implications that reinforce the effectiveness of ethics training.

Korean Red Ginseng protects dopaminergic neurons by suppressing the cleavage of p35 to p25 in a Parkinson's disease mouse model

Jun, Ye Lee,Bae, Chang-Hwan,Kim, Dongsoo,Koo, Sungtae,Kim, Seungtae The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.2

Background: Ginseng is known to have antiapoptotic, anti-inflammatory, and antioxidant effects. The present study investigated a possible role of Korean Red Ginseng (KRG) in suppressing dopaminergic neuronal cell death and the cleavage of p35 to p25 in the substantia nigra (SN) and striatum (ST) using a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease mouse model. Methods: Ten-week-old male C57BL/6 mice were injected intraperitoneally with 30 mg/kg of MPTP at 24-h intervals for 5 d, and then administered KRG (1 mg/kg, 10 mg/kg, or 100 mg/kg) once a day for 12 consecutive days from the first injection. Pole tests were performed to assess the motor function of the mice, dopaminergic neuronal survival in the SN and ST was evaluated using tyrosine hydroxylase-immunohistochemistry, and the expressions of cyclin-dependent kinase 5 (Cdk5), p35, and p25 in the SN and ST were measured using Western blotting. Results: MPTP administration caused behavioral impairment, dopaminergic neuronal death, increased Cdk5 and p25 expression, and decreased p35 expression in the nigrostriatal system of mice, whereas KRG dose-dependently alleviated these MPTP-induced changes. Conclusion: These results indicate that KRG can inhibit MPTP-induced dopaminergic neuronal death and suppress the cleavage of p35 to p25 in the SN and the ST, suggesting a possible role for KRG in the treatment of Parkinson's disease.