Optimizing the chlorine evolution reaction performance of Co(OH)2 catalyst for enhanced antifouling ability

Yating Peng,Peng Wang,Jiawei Li,Jiajia Wu,Feng Lin,Dun Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

Underwater optical instruments are commonly plagued by biofouling. As one of the most useful antifoulingstrategies, electrochemical chlorination is able to prevent biofouling efficiently, but the limited activityand selectivity in chlorine evolution reaction (CER) restrict its practical applications. To address thisproblem, we focus on the optimization of CER performance of cobalt-based catalysts. In this work, weprepared Co(OH)2 with different forms, morphologies, and intercalations. By adopting linear sweepvoltammetry and electrochemical active surface area analysis, it is demonstrated that the a-Co(OH)2exhibits better CER performance than b-Co(OH)2 does, and the manipulation of a-Co(OH)2 morphologycan help to further enhance CER performance. Meanwhile, the intercalation of a-Co(OH)2 plays a minorrole in the CER. Furthermore, based on the screened synthetic condition, the optimized a-Co(OH)2 wasapplied for practical antifouling examination, and its excellent antifouling ability was verified by theinvestigation on bacteria attachment and glass transmittance. The findings in this work not only enrichour understanding on the effects of synthetic conditions on CER performance of cobalt-based catalysts,but also provide useful insights into the development of electrochemical antifouling strategies.

Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus

Yating Chen,Kaichuang Shi,Huixin Liu,Yanwen Yin,Jing Zhao,Feng Long,Wenjun Lu,Hongbin Si 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6

Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5′ untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 100 copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.

Accelerated Life Test Study on Thyristors of HVDC Converter Valve

Cuicui Liu,Ning Liang,Yating Gou,Jiachen Tian,Fang Zhuo,Feng Wang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

As the key component of HVDC converter valve system, thyristors have vital impact on the reliability of the entire HVDC transmission project. In order to effectively obtain the life model of thyristor, this paper uses the existing accelerated life test theory, combined with the operating conditions of the converter valve and the relevant characteristics of the thyristors, to deeply analyse the several stress levels that have the most serious impact on the life of the thyristors. An accelerated life test scheme that can effectively guide the life model of the thyristor, according to the test data collected by this test method, the thyristor junction temperature life model under the short-circuit current stress is established, and compared with the thyristor failure rate in the practical engineering, the results show the feasibility of the test scheme and the correctness of the life model.

Genome-wide identification and functional prediction of silicon (Si) transporters in poplar (Populus trichocarpa)

Hassan Md Mahmudul,Martin Samir,Feng Kai,Yates Timothy B.,Yuan Guoliang,Martin Madhavi Z.,Martin Stanton,Muchero Wellington,Griffiths Natalie A.,Weston David J.,Yang Xiaohan 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2

Silicon (Si) enhances plant tolerance to various biotic and abiotic stressors such as salinity, drought, and heat. In addition, Si can be biomineralized within plants to form organic carbon-containing phytoliths that can have ecosystem-level consequences by contributing to long-term carbon sequestration. Si is taken up and transported in plants via different transporter proteins such as influx transporters (e.g., Lsi1, Lsi6) and efflux transporters (e.g., Lsi2). Additionally, the imported Si can be deposited in plant leaves via silicification process using the Siliplant 1 (e.g., Slp1) protein. Functional homologs of these proteins have been reported in different food crops. Here, we performed a genome-wide analysis to identify different Si transporters and Slp1 homologs in the bioenergy crop poplar (Populus trichocarpa Torr. and A. Gray ex W. Hook). We identified one channel-type Si influx transporter (PtLsi1; Potri.017G083300), one Si efflux transporter (PtLsi2; Potri.012G144000) and two proteins like Slp1 (PtSlp1a; Potri.004G168600 and PtSlp1b; Potri.009G129900) in the P. trichocarpa genome. We found a unique sequence (KPKPPVFKPPPVPI) in PtSlp1a which is repeated six times. Repeated presence of this sequence in PtSlp1a indicates that this protein might be important for silicification processes in P. trichocarpa. The mutation profiles of different Si transporters in a P. trichocarpa genome-wide association study population identified significant and impactful mutations in Potri.004G168600 and Potri.009G129900. Using a publically accessible database (http://bar.utoronto.ca/eplant_poplar/), digital expression analysis of the putative Si transporters in P. trichocarpa found low to moderate expression in the anticipated tissues, such as roots and leaves. Subcellular localization analysis found that PtLsi1/PtLsi2 are localized in the plasma membrane, whereas PtSlp1a/PtSlp1b are found in the extracellular spaces. Protein–Protein interaction analysis of PtLsi1/PtLsi2 identified Delta-1-pyrroline-5-carboxylate synthase (P5CS) as one of the main interacting partners of PtLsi2, which plays a key role in proline biosynthesis. Proline is a well-known participant in biotic and abiotic stress tolerance in plants. These findings will reinforce future efforts to modify Si accumulation for enhancing plant stress tolerance and carbon sequestration in poplar.

OsFKBP42b Regulates Rice Growth and Development Through Interacting with OsABCB1 and OsABCB14

Di Wang,Yingjie Wang,Gen Pan,Yanyan Wang,Guizhi Wang,Wenjing Chen,Yating Feng,Xi Liu 한국식물학회 2023 Journal of Plant Biology Vol.66 No.4

FK506 binding proteins, a member of peptidyl-prolyl cis-trans isomerase (PPIase), are essential for plant growth; however, the molecular function of FKBPs remains largely unknown. In this report, we isolated and identified an FK506 binding protein, OsFKBP42b, that regulates rice growth and development. The OsFKBP42b mutation led to reductions in plant height, panicle length, and seed setting rate. Scanning electron microscopy of the wild-type and the mutant stems showed no difference in cell size. OsFKBP42b was expressed in rice various organs, especially in panicles, leaves, and stems. Subcellular localization suggested that OsFKBP42b was a plasma membrane protein. Furthermore, we found that OsFKBP42b interacted with the ATP binding cassette B proteins, OsABCB1 and OsABCB14, by yeast two-hybrid and bimolecular fluorescence complementation assays. Taken together, our results provide new insights into OsFKBP42b in rice.