Genome-wide association study for intramuscular fat content in Chinese Lulai black pigs

Yanping Wang,Chao Ning,Cheng Wang,Jianfeng Guo,Jiying Wang,Ying Wu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.5

Objective: Intramuscular fat (IMF) content plays an important role in meat quality. Identification of single nucleotide polymorphisms (SNPs) and genes related to pig IMF, especially using pig populations with high IMF content variation, can help to establish novel molecular breeding tools for optimizing IMF in pork and unveil the mechanisms that underlie fat metabolism. Methods: We collected muscle samples of 453 Chinese Lulai black pigs, measured IMF content by Soxhlet petroleum-ether extraction method, and genotyped genome-wide SNPs using GeneSeek Genomic Profiler Porcine HD BeadChip. Then a genome-wide association study was performed using a linear mixed model implemented in the GEMMA software. Results: A total of 43 SNPs were identified to be significantly associated with IMF content by the cutoff p<0.001. Among these significant SNPs, the greatest number of SNPs (n = 19) were detected on Chr.9, and two linkage disequilibrium blocks were formed among them. Additionally, 17 significant SNPs are mapped to previously reported quantitative trait loci (QTLs) of IMF and confirmed previous QTLs studies. Forty-two annotated genes centering these significant SNPs were obtained from Ensembl database. Overrepresentation test of pathways and gene ontology (GO) terms revealed some enriched reactome pathways and GO terms, which mainly involved regulation of basic material transport, energy metabolic process and signaling pathway. Conclusion: These findings improve our understanding of the genetic architecture of IMF content in pork and facilitate the follow-up study of fine-mapping genes that influence fat deposition in muscle.

Development of a 120 Hz 110 ultra-high-definition a-Si liquid crystal display panel

Yanping Liao,Xibin Shao,Yufan Du,송용지,Weihao Hu,Zhenyu Zhang,Yuqiong Chen,Ying Wang,Qing Ma,윤대근,Dan Wang,Jianfeng Yuan,Hongjiang Wu,Zongjie Guo,Zhaohui Hao,Ji Zhang,Jing Lv 한국정보디스플레이학회 2014 Journal of information display Vol.15 No.2

A prototype 120 Hz 110-inch ultra-high-definition a-Si liquid crystal display (LCD) panel was successfully developed using the BOE exclusive advanced superdimensional switching technology. This LCD has a panel consisting of 3840 × 2160 pixels, four times the number of pixels in the conventional full-high-definition LCD. For the brightness and contrast ratio, 1000 nits and 1200:1 have been achieved, respectively. Additionally, the liquid crystal charging time has been extended through quad area driving and 2G2D line structures. Furthermore, the local dimming and mirror-scanning techniques are applied to realize excellent image display. Finally, the system utilizes 3D shutter glasses, allowing the viewer to achieve the highest sense of realness and immersion.

High-density single nucleotide polymorphism chip-based conservation genetic analysis of indigenous pig breeds from Shandong Province, China

Wang, Yanping,Zhao, Xueyan,Wang, Cheng,Wang, Wenwen,Zhang, Qin,Wu, Ying,Wang, Jiying Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: Shandong indigenous pig breeds are important Chinese pig resources. Their progressive population decline in recent decades has attracted attention towards their conservation. Conservation genetics of these indigenous breeds are essential for developing a conservation and utilization scheme. Methods: A high-density single nucleotide polymorphism (HD-SNP) chip-based comparative analysis of genetic characteristics was performed for seven Shandong indigenous pig breeds in the context of five Western commercial breeds. Results: The results showed that Shandong indigenous pig breeds varied greatly in genetic diversity, effective population size, inbreeding level, and genetic distance with the Western commercial breeds. Specifically, Laiwu and Dapulian displayed low genetic diversity, and had a genetically distant relationship with the Western commercial breeds (average F statistics [F_ST] value of 0.3226 and 0.2666, respectively). Contrastingly, the other five breeds (Yantai, Licha, Yimeng, Wulain, and Heigai) displayed high genetic diversity within breed and had some extent of mixture pattern with the Western commercial breeds, especially Duroc and Landrace (F_ST values from 0.1043 to 0.2536). Furthermore, intensive gene flow was discovered among the seven Shandong indigenous breeds, particularly Wulian, Licha, and Heigai, as indicated by the large cluster formed in the principal component analysis scatterplot and small population differentiation (average of 0.1253) among them. Conclusion: Our study advances the understanding of genetic characteristics of Shandong indigenous breeds and provides essential information for developing an appropriate conservation and utilization scheme for these breeds.

A Novel Interleaving Control Scheme for Boost Converters Operating in Critical Conduction Mode

Xu Yang,Yanping Ying,Wenjie Chen 전력전자학회 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.2

Interleaving techniques are widely used to reduce input/output ripples and to increase the power capacity of boost converters operating in critical conduction mode. Two types of phase-shift control schemes are studied in this paper, the turn-on time shifting method and the turn-off time shifting method. It is found that although the turn-off time shifting method exhibits better performance, it suffers from sub-harmonic oscillations at high input voltages. To solve this problem, an intensive quantitative analysis of the sub-harmonic oscillation phenomenon is made in this paper. Based upon that, a novel modified turn off time shifting control scheme for interleaved boost converters operating in critical conduction mode is proposed. An important advantage of this scheme is that both the master phase and the slave phase can operate stably in critical conduction mode without any oscillations in the full input voltage range. This method is implemented with a FPGA based digital PWM control platform, and tests were carried out on a two-phase interleaved boost PFC converter prototype. Experimental results demonstrated the feasibility and performance of the proposed phase-shift control scheme.

A Novel Interleaving Control Scheme for Boost Converters Operating in Critical Conduction Mode

Yang, Xu,Ying, Yanping,Chen, Wenjie The Korean Institute of Power Electronics 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.2

Interleaving techniques are widely used to reduce input/output ripples and to increase the power capacity of boost converters operating in critical conduction mode. Two types of phase-shift control schemes are studied in this paper, the turn-on time shifting method and the turn-off time shifting method. It is found that although the turn-off time shifting method exhibits better performance, it suffers from sub-harmonic oscillations at high input voltages. To solve this problem, an intensive quantitative analysis of the sub-harmonic oscillation phenomenon is made in this paper. Based upon that, a novel modified turn off time shifting control scheme for interleaved boost converters operating in critical conduction mode is proposed. An important advantage of this scheme is that both the master phase and the slave phase can operate stably in critical conduction mode without any oscillations in the full input voltage range. This method is implemented with a FPGA based digital PWM control platform, and tests were carried out on a two-phase interleaved boost PFC converter prototype. Experimental results demonstrated the feasibility and performance of the proposed phase-shift control scheme.

Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation

Wang, Jiying,Wang, Yanping,Wang, Huaizhong,Hao, Xiaojing,Wu, Ying,Guo, Jianfeng Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4

Investigating gene expression of immune cells of whole blood or peripheral blood mononuclear cells (PBMC) under polyinosinic:polycytidylic acid (poly I:C) stimulation is valuable for understanding the immune response of organism to RNA viruses. Quantitative real-time PCR (qRT-PCR) is a standard method for quantification of gene expression studies. However, the reliability of qRT-PCR data critically depends on proper selection of reference genes. In the study, using two different analysis programs, geNorm and NormFinder, we systematically evaluated the gene expression stability of six candidate reference genes (GAPDH, ACTB, B2M, RPL4, TBP, and PPIA) in samples of whole blood and PBMC with or without poly I:C stimulation. Generally, the six candidate genes performed a similar trend of expression stability in the samples of whole blood and PBMC, but more stably expressed in whole blood than in PBMC. geNorm ranked B2M and PPIA as the best combination for gene expression normalization, while according to NormFinder, TBP was ranked as the most stable reference gene, followed by B2M and PPIA. Comprehensively considering the results from the two programs, we recommended using the geometric mean of the three genes, TBP, PPIA and B2M, to normalize the gene expression of whole blood and PBMC with poly I:C stimulation. Our study is the first detailed survey of the gene expression stability in whole blood and PBMC with or without poly I:C stimulation and should be helpful for investigating the molecular mechanism involved in porcine whole blood and PBMC in response to poly I:C stimulation.