KDM3A promotes oral squamous cell carcinoma cell proliferation and invasion via H3K9me2 demethylation-activated DCLK1

Yang Lei,Zhang Qiqiong,Yang Qiuye 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Oral squamous cell carcinoma (OSCC) is a frequently-diagnosed malignancy with high potential for proliferation and invasion. Histone methylation is known as a crucial mechanism that regulates pathological processes in various cancers, including OSCC. Objective: This study sought to delve into the molecular mechanism of lysine demethylase 3 A (KDM3A) in OSCC cell proliferation and invasion. Methods: Expression levels of KDM3A, lysin-9 of di-methylated histone H3 (H3K9me2), and doublecortin-like kinase 1 (DCLK1) in cells were determined by reverse-transcription quantitative polymerase chain reaction or Western blot analysis. Cell proliferation and invasion were evaluated by cell counting kit-8, colony formation, and Transwell assays. The enrichment of KDM3A and H3K9me2 on the DCLK1 promoter was determined by chromatin immunoprecipitation assay. The functional rescue experiment was performed with DCLK1 overexpression vector and si-KDM3A in CAL-27 and SCC-9 cells. Results: KDM3A was elevated in OSCC cells. KDM3A knockdown suppressed OSCC proliferation and invasion, along with increased H3K9me2 level in OSCC cells. KDM3A and H3K9me2 were enriched on the DCLK1 promoter and inhibiting H3K9me2 improved DCLK1 expression levels. DCLK1 overexpression neutralized the inhibition of KDM3A knockdown on OSCC proliferation and invasion. Conclusions: KDM3A facilitated OSCC proliferation and invasion by eliminating H3K9me2 to upregulate DCLK1 expression levels.

Complete genome sequence of Lactiplantibacillus plantarum ST, a potential probiotic strain with antibacterial properties

( Shujuan Yang ),( Chenglin Deng ),( Yao Li ),( Weicheng Li ),( Qiong Wu ),( Zhihong Sun ),( Zhenhui Cao ),( Qiuye Lin ) 한국축산학회 2022 한국축산학회지 Vol.64 No.1

Lactiplantibacillus plantarum (L. plantarum) ST was isolated from De’ang pickled tea in Yunnan Province, China. The genomes of strain ST were fully sequenced and analyzed using the PacBio RS II sequencing system. Our previous study has shown that L. plantarum ST is a potential probiotic strain. It had strong tolerance in the simulated artificial gastrointestinal tract, and in the antagonism tests, this strain showed strong antibacterial activity. Therefore, as a probiotic, it may be used in animal breeding. L. plantarum ST genome was composed of 1 circular chromosome and 7 plasmids. The length of the whole genome was 3320817 bp, and the annular chromosome size was 3058984 bp, guanine + cytosine (G ± C) content (%) was 44.76%, which contained 2945 protein-coding sequences (CDS). This study will contribute to a further comprehensive understanding of L. Plantarum ST at the genomic level and provide a theoretical basis for its future application in animal breeding.

On the transcendental entire solutions of a class of differential equations

Weiran Lu,Qiuying Li,Chungchun Yang 대한수학회 2014 대한수학회보 Vol.51 No.5

In this paper, we consider the differential equation F′ − Q1 = Reα(F − Q2), where Q1 and Q2 are polynomials with Q1Q2 6≡ 0,R is a rational function and α is an entire function. We consider solutions of the form F = fn, where f is an entire function and n ≥ 2 is an integer, and we prove that if f is a transcendental entire function, then Q1 Q2 is a polynomial and f′ = Q1 nQ2 f. This theorem improves some known results and answers an open question raised in [16].

ON THE TRANSCENDENTAL ENTIRE SOLUTIONS OF A CLASS OF DIFFERENTIAL EQUATIONS

Lu, Weiran,Li, Qiuying,Yang, Chungchun Korean Mathematical Society 2014 대한수학회보 Vol.51 No.5

In this paper, we consider the differential equation $$F^{\prime}-Q_1=Re^{\alpha}(F-Q_2)$$, where $Q_1$ and $Q_2$ are polynomials with $Q_1Q_2{\neq}0$, R is a rational function and ${\alpha}$ is an entire function. We consider solutions of the form $F=f^n$, where f is an entire function and $n{\geq}2$ is an integer, and we prove that if f is a transcendental entire function, then $\frac{Q_1}{Q_2}$ is a polynomial and $f^{\prime}=\frac{Q_1}{nQ_2}f$. This theorem improves some known results and answers an open question raised in [16].

Synthesis and biological evaluation of novel fluconazole analogues bearing 1,3,4-oxadiazole moiety as potent antifungal agents

Jun Liao,Fan Yang,Lei Zhang,Xiaoyun Chai,Qing-Jie Zhao,Shichong Yu,Yan Zou,Qingguo Meng,Qiuye Wu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.4

A novel series of fluconazole based mimicsincorporating 1,3,4-oxadiazole moiety were designed andsynthesized. All the title compounds were characterized by1H-NMR, 13C-NMR, and Q-TOF-MS. Preliminary resultsrevealed that most of analogues exhibited significant antifungalactivity against seven pathogenic fungi. Compounds9g and 9k (MIC80 B 0.125 lg/mL, respectively) werefound more potent than the positive controls itraconazoleand fluconazole as broad-spectrum antifungal agents. Theobserved docking results showed that the 1,3,4-oxadiazolemoiety enhanced the affinity binding to the cytochromeP450 14a-demethylase (CYP51).

Tomato Yellow Leaf Curl China Virus Impairs Photosynthesis in the Infected Nicotiana benthamiana with βC1 as an Aggravating Factor

Farooq, Tahir,Liu, Dandan,Zhou, Xueping,Yang, Qiuying The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.5

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. ${\beta}C1$ is a viral factor encoded by the betasatellite DNA ($DNA{\beta}$) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of ${\beta}C1$ on plants' photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TY-LCCNV-infected and ${\beta}C1$ transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A-infected, TYLCCNV A plus $DNA{\beta}$ (TYLCCNV A + ${\beta}$)-infected and ${\beta}C1$ transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, $F_v/F_m$, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of ${\beta}C1$ aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + ${\beta}$ infection and at the vegetative stage of ${\beta}C1$ transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of ${\beta}C1$ in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.

Tomato Yellow Leaf Curl China Virus Impairs Photosynthesis in the Infected Nicotiana benthamiana with βC1 as an Aggravating Factor

Tahir Farooq,Dandan Liu,Xueping Zhou,Qiuying Yang 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.5

Tomato yellow leaf curl China virus is a species of the widespread geminiviruses. The infection of Nicotiana benthamiana by Tomato yellow leaf curl China virus (TYLCCNV) causes a reduction in photosynthetic activity, which is part of the viral symptoms. βC1 is a viral factor encoded by the betasatellite DNA (DNAβ) accompanying TYLCCNV. It is a major viral pathogenicity factor of TYLCCNV. To elucidate the effect of βC1 on plants’ photosynthesis, we measured the relative chlorophyll (Chl) content and Chl fluorescence in TYLCCNV- infected and βC1 transgenic N. benthamiana plants. The results showed that Chl content is reduced in TYLCCNV A–infected, TYLCCNV A plus DNAβ (TYLCCNV A + β)–infected and βC1 transgenic plants. Further, changes in Chl fluorescence parameters, such as electron transport rate, Fv/Fm, NPQ, and qP, revealed that photosynthetic efficiency is compromised in the aforementioned N. benthamiana plants. The presense of βC1 aggravated the decrease of Chl content and photosynthetic efficiency during viral infection. Additionally, the real-time quantitative PCR analysis of oxygen evolving complex genes in photosystem II, such as PsbO, PsbP, PsbQ, and PsbR, showed a significant reduction of the relative expression of these genes at the late stage of TYLCCNV A + β infection and at the vegetative stage of βC1 transgenic N. benthamiana plants. In summary, this study revealed the pathogenicity of TYLCCNV in photosynthesis and disclosed the effect of βC1 in exacerbating the damage in photosynthesis efficiency by TYLCCNV infection.