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      • KCI등재

        Enhancing Cellulase Production in Thermophilic Fungus Myceliophthora thermophila ATCC42464 by RNA Interference of cre1 Gene Expression

        ( Fan Yang ),( Yanfen Gong ),( Gang Liu ),( Shengming Zhao ),( Juan Wang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7

        The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.

      • SCIESCOPUSKCI등재

        Peste des petits ruminants virus infection induces endoplasmic reticulum stress and apoptosis via IRE1-XBP1 and IRE1-JNK signaling pathways

        Shuyi Yuan,Yanfen Liu,Yun Mu,Yongshen Kuang,Shaohong Chen,Yun-Tao Zhao,You Liu The Korean Society of Veterinary Science 2024 Journal of Veterinary Science Vol.25 No.1

        Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

      • SCIESCOPUSKCI등재

        Peste des petits ruminants virus infection induces endoplasmic reticulum stress and apoptosis via IRE1-XBP1 and IRE1-JNK signaling pathways

        Shuyi Yuan,Yanfen Liu,Yun Mu,Yongshen Kuang,Shaohong Chen,Yun-Tao Zhao,You Liu 대한수의학회 2024 Journal of Veterinary Science Vol.25 No.2

        Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.

      • KCI등재

        Low-temperature strategy for vapor phase hydrothermal synthesis of C\N\S-doped TiO2 nanorod arrays with enhanced photoelectrochemical and photocatalytic activity

        Hai Yu,Miao Zhang,Yanfen Wang,Janguo Lv,Yanmei Liu,Gang He,Zhaoqi Sun 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.98 No.-

        In this study, a material with high photocatalytic activity was synthesized using ternary C/N/S-dopedTiO2 nanorod array (TiO2); this was done using a practical and straightforward vapor-phase hydrothermal(VPH) method at a low temperature. The effect of C/N/S content on TiO2 morphology, optical,photocatalytic and photoelectrochemical (PCE) properties of the material was investigated by varying thequality of thiourea. C/N/S-TiO2 reduced the bonding rate of electron-hole pairs and enhances visible lightabsorption, photocatalytic, and PCE properties. The C/N/S doping could significantly adjust theabsorption cut-off wavelengths (407 602 nm) and shorten the bandgap (3.04 2.18 eV) of TiO2. Undersimulated sunlight, 8-C/N/S-TiO2 had the highest photocatalytic efficiency of 97.6% for methylene blue(MB) in 150 min with a rate constant of 0.0192 min 1, which is approximately four times that of TiO2(0.005 min 1). The 8-C/N/S-TiO2 photoelectrode had the lowest transfer resistance for interfacial chargesand highest transient photocurrent of 33.5 mA/cm2, which isfive times higher than that of TiO2 (6.6 mA/cm2). The 8-C/N/S-TiO2 exhibits the most extensive PCE behavior as a photoelectrode, and has a currentdensity of 38.2 mA/cm2 at 2.5VRHE, which is about two times higher than TiO2 (19.1 mA/cm2). Thefavorable sunlight-driven photocatalytic activity is probably due to the synergistic effect of C/N/S-doping,which shifts the valence band maximum of TiO2 upward. This provides new ideas for future solar cellsthat can use dye-sensitized TiO2 nanorod arrays as photoanodes. It is noteworthy that VPH is a veryeffective strategy for fabricating semiconductors doped with multiple nonmetallic elements.

      • KCI등재

        Transmit Precoder Design for Two-User Broadcast Channel with Statistical and Delayed CSIT

        ( Yanjing Sun ),( Shu Zhou ),( Qi Cao ),( Yanfen Wang ),( Wen Liu ),( Xiaoguang Zhang ) 한국인터넷정보학회 2018 KSII Transactions on Internet and Information Syst Vol.12 No.5

        Recent studies have revealed the efficacy of incorporating delayed channel state information at transmit side (CSIT) in transmission scheme design. This paper focuses on transmit precoder design to maximize the ergodic sum-rate in a two-user Multiple-Input Single-Output (MISO) system with delayed and statistical CSIT. A new transmit strategy which precodes signals in all transmit slots is proposed in this paper, denoted as all time-slots precoding Alternative MAT (AAMAT). There is a common procedure in conventional delayed-CSIT based schemes, which is retransmitting the overheard interferences. Since the retransmitting signal is intended to both users, all previous schemes tend to use only one antenna. We however figure out an improvement in spectral efficiency could be realized if all antennas can be utilized. In this paper, we detail the design of the procoder which enabling all antennas and also we compute a lower bound of the ergodic sum-rate in an ideal condition. In addition, simulation results demonstrate the superiority of our proposed scheme.

      • KCI등재

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