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롤러스케이트 선수의 운동상해발생과 예방에 대한 조사 연구
김동희,김도수,채양석,이하얀,김선호,조성채 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1
The study was intended to research the fact of roller skate players injury. For the study, kinds and parts of injuries, skills to be hurted, facility, injured time, cause, remedy, mental response and protecting were investigated through 182 roller skate players. The results were as follows : 1. The injury body part was showed that leg was the highest and followed by knee and ankle. 2. In the cause of injury was showed as follows: skills on corner work, bank track with concrete, 200m distance. 3. In the general cause of injury was showed: chronic fatigue by over training. 4. In the treatment and participation period after injury outbreak were showed: treatment period was toward the inside 1 week and participation to play during the treatment. 5. To the injury prevention players responded warm up, and cool down and mental-concentration were needed. Supervisor, coach and players responded knee protector wearing was needed in elementary school players and helmet-chin protector wearing in general players. The game rule revision was not necessary and the agree and disagree for ground unity to resemble. Almost players to be like concrete ground.
Yan Sun,이승민,구본진,문명진 한국통합생물학회 2023 Animal cells and systems Vol.27 No.1
The water-soluble glue substance of the capture threads in Trichonephila clavata is solely producedfrom two pairs of aggregate silk glands. During the web glue production, secretory vesicles weresynthesized via the extensive rough endoplasmic reticulum of epithelial cells. Unlike the clearlydescribed fibrous web production in spiders, the process of aqueous web glue productionappears to involve either a condensing or a packaging step by the Golgi complex. In particular,the fine structure of secretory vesicles varies from cell to cell and may represent the secretorycycle. The electron-dense multivesicular bodies were clearly visible as discrete droplets, and themature secretory product in the glandular epithelium appeared as a spherical vacuole grown byfusion with surrounding small vesicles. Our fine structural observation reveals that the secretionoccurs when the release of secreted material involves the loss of part of the cytoplasm. Thebleb along the luminal surface of the secretory cells and membrane-bound extracellular vesicleswhich pinched off from the cell suggests that the secretory product is released by themechanism of apocrine secretion.
Sun, Yan,Zhang, Yong-Jun,Kong, Xiang-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9
Background: This study aimed to explore potential associations between single nucleotide polymorphisms (SNPs) of the x-ray repair cross-complementing group 1 (XRCC1) and cleft lip and palate transmembrane protein 1-like (CLPTM1L) and non-small cell lung cancer (NSCLC) susceptibility in non-smoker Chinese patients. Methods: A total of 200 NSCLC patients and 200 healthy controls with matched age and gender were recruited for genotyping of XRCC1 SNPs (rs2256507 and rs1001581) and CLPTM1L SNPs (rs401681 and rs4975616). Association of these SNPs with NSCLC risk was evaluated by computing the odds ratio (OR) and 95% confidence interval (CI) from multivariate unconditional logistic regression analyses with adjustment for gender and age. Results: The frequencies of genotype and allele in these four loci (rs2256507, rs1001581, rs401681, and rs4975616) were not significantly different between the cases and controls, or between either of the histological subgroups (adenocarcinoma and squamous cell carcinoma) and controls. Conclusions: Although these SNPs are associated with NSCLC risk in patients with a tobacco-smoking habit, this study demonstrated that XRCC1 and CLPTM1L gene SPNs are not linked with NSCLC risk in non-smoking patients, indicating that molecular mechanisms of NSCLC betwee tobacco smokers and non-smokers may be different. Future studies are needed to uncover the underlying molecular mechanisms for NSCLC in non-smokers.
Sun, Yufei,Feng, Guifang,Zheng, Yan,Liu, Shu,Zhang, Yan,Pi, Zifeng,Song, Fengrui,Liu, Zhiqiang The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: Exploring the pharmacokinetic (PK) changes of various active components of single herbs and their combinations is necessary to elucidate the compatibility mechanism. However, the lack of chemical standards and low concentrations of multiple active ingredients in the biological matrix restrict PK studies. Methods: A putative multiple reaction monitoring strategy based on liquid chromatography coupled with mass spectrometry (LC-MS) was developed to extend the PK scopes of quantification without resorting to the use of chemical standards. First, the compounds studied, including components with available reference standard (ARS) and components lacking reference standard (LRS), were preclassified to several groups according to their chemical structures. Herb decoctions were then subjected to ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry analysis with appropriate collision energy (CE) in MS<sup>2</sup> mode. Finally, multiple reaction monitoring transitions transformed from MS<sup>2</sup> of ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry were used for ultrahigh-performance liquid chromatography coupled with triple quadrupole mass spectrometry to obtain the mass responses of LRS components. LRS components quantification was further performed by developing an assistive group-dependent semiquantitative method. Results: The developed method was exemplified by the comparative PK process of single herbs Radix Ginseng (RG), Radix Polygala (RP), and their combinations (RG-RP). Significant changes in PK parameters were observed before and after combination. Conclusion: Results indicated that Traditional Chinese Medicine combinations can produce synergistic effects and diminish possible toxic effects, thereby reflecting the advantages of compatibility. The proposed strategy can solve the quantitative problem of LRS and extend the scopes of PK studies.
Sun, Yan Lin,Hong, Soon-Kwan The Korean Society of Plant Biotechnology 2009 식물생명공학회지 Vol.36 No.3
The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.
Sun, Yan-Lin,Hong, Soon-Kwan The Plant Resources Society of Korea 2010 한국자원식물학회지 Vol.23 No.6
Genomes of clusters of related eukaryotes are now being sequenced at an increasing rate. In this paper, we developed an accurate, low-cost method for annotation of gene prediction and exon-intron structure. The gene prediction was adapted for delta 1-pyrroline-5-carboxylate-synthetase (p5cs) gene from China wild-type of the halophytic Leymus chinensis (Trin.), naturally adapted to highly-alkali soils. Due to complex adaptive mechanisms in halophytes, more attentions are being paid on the regulatory elements of stress adaptation in halophytes. P5CS encodes delta 1-pyrroline-5-carboxylate-synthetase, a key regulatory enzyme involved in the biosynthesis of proline, that has direct correlation with proline accumulation in vivo and positive relationship with stress tolerance. Using analysis of reverse transcription-polymerase chain reaction (RT-PCR) and PCR, and direct sequencing, 1076 base pairs (bp) of cDNA in length and 2396 bp of genomic DNA in length were obtained from direct sequencing results. Through gene prediction and exon-intron structure verification, the full-length of cDNA sequence was divided into eight parts, with seven parts of intron insertion. The average lengths of determinated coding regions and non-coding regions were 154.17 bp and 188.57 bp, respectively. Nearly all splice sites displayed GT as the donor sites at the 5' end of intron region, and 71.43% displayed AG as the acceptor sites at the 3' end of intron region. We conclude that this method is a cost-effective way for obtaining an experimentally verified genome annotation.