Gene-metabolite network analysis in different nonalcoholic fatty liver disease phenotypes

Xiao-Lin Liu,Ya-Nan Ming,Jing-Yi Zhang,Xiao-Yu Chen,Min-De Zeng,Yi-Min Mao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

We sought to identify common key regulators and build a gene-metabolite network in different nonalcoholic fatty liver disease (NAFLD) phenotypes. We used a high-fat diet (HFD), a methionine-choline-deficient diet (MCDD) and streptozocin (STZ) to establish nonalcoholic fatty liver (NAFL), nonalcoholic steatohepatitis (NASH) and NAFL+type 2 diabetes mellitus (T2DM) in rat models, respectively. Transcriptomics and metabolomics analyses were performed in rat livers and serum. A functional network-based regulation model was constructed using Cytoscape with information derived from transcriptomics and metabolomics. The results revealed that 96 genes, 17 liver metabolites and 4 serum metabolites consistently changed in different NAFLD phenotypes (42-fold, Po0.05). Gene-metabolite network analysis identified ccl2 and jun as hubs with the largest connections to other genes, which were mainly involved in tumor necrosis factor, P53, nuclear factor-kappa B, chemokine, peroxisome proliferator activated receptor and Toll-like receptor signaling pathways. The specifically regulated genes and metabolites in different NAFLD phenotypes constructed their own networks, which were mainly involved in the lipid and fatty acid metabolism in HFD models, the inflammatory and immune response in MCDD models, and the AMPK signaling pathway and response to insulin in HFD+STZ models. Our study identified networks showing the general and specific characteristics in different NAFLD phenotypes, complementing the genetic and metabolic features in NAFLD with hepatic and extra-hepatic manifestations.

Matrine Reduces Proliferation of Human Lung Cancer Cells by Inducing Apoptosis and Changing miRNA Expression Profiles

Liu, Yong-Qi,Li, Yi,Qin, Jie,Wang, Qian,She, Ya-Li,Luo, Ya-Li,He, Jian-Xin,Li, Jing-Ya,Xie, Xiao-Dong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Matrine, a main active component extracted from dry roots of Sophora flavecens, has been reported to exert antitumor effects on A549 human non-small lung cancer cells, but its mechanisms of action remain unclear. To determine effects of matrine on proliferation of A549 cells and assess possible mechanisms, MTT assays were employed to detect cytotoxicity, along with o flow cytometric analysis of DNA content of nuclei of cells following staining with propidium iodide to analyze cell cycle distribution. Western blotting was performed to determined expression levels of Bax, Bcl-2, VEGF and HDAC1, while a microarray was used to assessed changes of miRNA profiles. In the MTT assay, matrine suppressed growth of human lung cancer cell A549 in a dose- and timedependent manner at doses of 0.25-2.5 mg/ml for 24h, 48h or 72h. Matrine induced cell cycle arrest in G0/G1 phase and decreased the G2/M phase, while down-regulating the expression of Bcl2 protein, leading to a reduction in the Bcl-2/Bax ratio. In addition, matrine down regulated the expression level of VEGF and HDAC1 of A549 cells. Microarray analysis demonstrated that matrine altered the expression level of miRNAs compared with untreated control A549 cells. In conclusion, matrine could inhibit proliferation of A549 cells, providing useful information for understanding anticancer mechanisms.

Enhancement of Butanol Tolerance and Butanol Yield in Clostridium acetobutylicum Mutant NT642 Obtained by Nitrogen Ion Beam Implantation

Xiao-Bo Liu,Qiu-Ya Gu,Xiao-Bin Yu,Wei Luo 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyperyield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.

Astragali Radix: comprehensive review of its botany, phytochemistry, pharmacology and clinical application

Ya-Xiao Liu,Xiao-Mei Song,Lin-Wei Dan,Jia-Mei Tang,Yi Jiang,Chong Deng,Dong-Dong Zhang,Yu-Ze Li,Wei Wang 대한약학회 2024 Archives of Pharmacal Research Vol.47 No.3

Astragali Radix ( A. Radix ) is the dried root of Astragalus membranaceus var. mongholicus (Bge) Hsiao or Astragalusmembranaceus (Fisch.) Bge., belonging to the family Leguminosae, which is mainly distributed in China. A. Radix hasbeen consumed as a tonic in China for more than 2000 years because of its medicinal eff ects of invigorating the spleenand replenishing qi. Currently, more than 400 natural compounds have been isolated and identifi ed from A. Radix , mainlyincluding saponins, fl avonoids, phenylpropanoids, alkaloids, and others. Modern pharmacological studies have shown thatA. Radix has anti-tumor, anti-infl ammatory, immunomodulatory, anti-atherosclerotic, cardioprotective, anti-hypertensive,and anti-aging eff ects. It has been clinically used in the treatment of tumors, cardiovascular diseases, and cerebrovascularcomplications associated with diabetes with few side eff ects and high safety. This paper reviewed the progress of researchon its chemical constituents, pharmacological eff ects, clinical applications, developing applications, and toxicology, whichprovides a basis for the better development and utilization of A. Radix .

Recurrence Season Impacts the Survival of Epithelial Ovarian Cancer Patients

Liu, Xiao-Hui,Man, Ya-Nan,Wu, Xiong-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background: Several studies indicated that the diagnosis season affects the prognosis of some cancers, such as examples in the prostate, colon and breast. This retrospective study aimed to investigate whether the diagnosis and recurrent season impacts the prognosis of epithelial ovarian cancer patients. Methods: From January 2005 to August 2010, 161 epithelial ovarian cancer patients were analyzed and followed up until August 2013. Kaplan-Meier survival curves and the log-rank test were used to make the survival analysis. Multivariate analysis was conducted to identify independent prognostic factors. Results: The prognostic factors of overall survival in epithelial ovarian cancer patients included age, clinical stage, pathological type, histological grade, residual disease after primary surgery, recurrent season and adjuvant chemotherapy cycles. Moreover, clinical stage, histological grade, residual disease after primary surgery, recurrent season and adjuvant chemotherapy cycles also impacted the progression-free survival of epithelial ovarian cancer patients. The diagnosis season did not have a significantly relationship with the survival of operable epithelial ovarian cancer patients. Median overall survival of patients with recurrent month from April to November was 47 months, which was longer (P < 0.001) than that of patients with recurrence month from December to March (19 months). Median progression-free survival of patients with recurrence month from April to November and December to March was 20 and 8 months, respectively (P < 0.001). Conclusion: The recurrence season impacts the survival of epithelial ovarian cancer patients. However, the diagnosed season does not appear to exert a significant influence.

Modified Procedures for ALPPS Based on a Risk-Reduced Strategy: Paralleled Clinical Evaluation at Multiple Institutions

Ya-Lin Kong,Ying Xing,Jie Li,Cheng-Li Liu,Xiao-Jun He,Cheng Wang,Jiang-Min Chen,Ling-Hong Kong,Xu Han,Hong-Yi Zhang 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.10

Purpose: We compared the clinical outcomes of modified procedures for associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) based on a risk-reduced strategy with those of classic ALPPS procedures in treating large liver carcinoma. Materials and Methods: Short-term outcomes, increases in future liver remnant (FLR) and functional FLR (FFLR), and overall survival (OS) were compared between 45 consecutive patients treated with modified ALPPS procedures and 34 patients treated with classic ALPPS procedures. Results: Clinical outcomes after the 1st-stage operation markedly improved with the modified procedures. Although the proportions of liver cirrhosis and hepatocellular carcinoma were higher in the modified group, the mortality and incidence of severe complications did not increase. FLR and FFLR hypertrophy at 1 week after the 1st-stage operation were similar in both groups; however, kinetic growth rates in the modified group were lower. OS rates were similar. Conclusion: Modified ALPPS procedures could be safely applied to provide long-term survival for patients with liver cirrhosis without sufficient FLR.

Effect of Minor Sc Addition on the Microstructure Evolution of Al–Cu–Li–Mg Alloy During Homogenization with Different Cooling Modes

Ya Tang,Daihong Xiao,Lanping Huang,Renxuan You,Xinyue Zhao,Nan Lin,Yunzhu Ma,Wensheng Liu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

The microstructure evolution and mechanical properties of Al–3.92Cu–1.10Li–0.68Mg–0.32Ag–0.23Mn–0.35Zn–0.11Zrand Al–3.92Cu–1.12Li–0.76Mg–0.29Ag–0.24Mn–0.24Zn–0.12Zr–0.083Sc (wt%) alloys during homogenization with differentcooling modes were comprehensively studied. It was clearly revealed that the minor Sc addition refined the grainsof as-cast Al–Cu–Li–Mg alloys through the formation of primary Al3(Sc, Zr) phases during the solidification process, andinduced Sc enrichment in the θ (Al2Cu) eutectic. After the homogenization treatment, the majority of coarse nonequilibriumeutectic phases at the grain boundaries dissolved into the α-Al matrix, leading to the relatively uniform distribution ofeach element. Sc-added alloy after homogenization treatments possessed more superior strength and ductility than Sc-freealloy. Compared to the furnace cooling mode, the air cooling mode could inhibit the precipitation of micron-sized coarse T1(Al2CuLi) phase and improve the mechanical properties of the alloys. After homogenization, the continuous Al2Cuphase inthe as-cast Sc-added alloy dissolved and transformed to an array of W-(Al, Cu, Sc) ternary phase, which was identified asAl6Cu6Scwith the body-centered tetragonal structure. The appearance of spherical Al6Cu6Scparticle could not damage themechanical properties of Al–Cu–Li–Mg alloys after the homogenization treatment. The tensile strength, yield strength andelongation of Sc-added alloy after the homogenization by air cooling were 390 MPa, 265 MPa and 10.8%, respectively. Theinvestigation of Al6Cu6Scphase offered a potential avenue to produce high-quality Sc-added 2xxx series alloys.

Synthesis, Crystal Structure and Characterization of Cu(II) and Cd(II) Coordination Compounds Based on Ligand 2-(3-(Pyridin-2-yl)-1H-pyrazol-1-yl)acetic Acid

Ya-Jun Zhang,Cui-Juan Wang,Kai-Li Mao,Xiao-Lei Liu,Shuai Huang,Yan Tong,Xian-Li Zhou 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.7

Two novel coordination compounds [Cu2(pypya)3(H2O)2]·Cl·(H2O)5 (1) and {[Cd(pypya)(ta)1/2]·H2O}n (2) (Hpypya=2-(3-(pyridin-2-yl)-1H-pyrazol-1-yl)acetic acid, H2ta=terephthalic acid) were synthesized and characterized by single X-ray diffraction. Structure determination reveals that complex 1 and complex 2 crystallize in the triclinic system, with the P-1 space group. The asymmetric unit of 1 contains two Cu(II) ions, and their coordination modes are different. These units of complex 1 are linked together via hydrogen bonds and π-π interactions, and the 3D structure of complex 1 was formed. Complex 2, a mononuclear Cd(II) coordination compound, has a 2D structure which was constructed via coordination bonds. TGA and fluorescence spectra analysis of complex 1 and complex 2 have also been studied. In addition, the geometry parameters of complex 1 have been optimized with the B3LYP method of density functional theory (DFT) to explain its coordination behavior. The electronic properties of the complex 1 and ligand Hpypya have been investigated based on the nature bond orbital (NBO) analysis at the B3LYP level of theory. The result verifies that the synergistic effect have occurred in the compound.

Synthesis and Optical Properties of the Semiconductor Lead Sulfide Nanobelts

Xiao-hong Yang,Qing-Sheng Wu*,Ya-Ping Ding,Jin-ku Liu 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.3

IL-13 R110Q, a Naturally Occurring IL-13 Polymorphism, Confers Enhanced Functional Activity in Cultured Human Bronchial Smooth Muscle Cells

Ya-fang He,Li Hua,Yi-xiao Bao,Quan-hua Liu,Yi Chu,Ding-zhu Fang 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6

Purpose: Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smoothmuscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the presentstudy, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparisonwith wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs). Methods: Culture supernatants and cell proteins were collectedfrom cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 h. Eotaxin released into hBSMC culture medium was determinedby ELISA. The expression levels of the high-affinity IgE receptor (FcεRI) α-chain, smooth muscle-specific actin alpha chain (α-SMA), smoothmuscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots. Results: Compared with WT IL-13, treatmentwith the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expressionlevels of α-SMA, SmMHC, calreticulin, and FcεRI α-chain. Conclusions: The results of the present study suggenst that the IL-13 R110Q variantmay enhance enhanced functional activities in hBSMCs.