Sequencing, Genomic Structure, Chromosomal Mapping and Association Study of the Porcine ADAMTS1 Gene with Litter Size

Yue, K.,Peng, J.,Zheng, R.,Li, J.L.,Chen, J.F.,Li, F.E.,Dai, L.H.,Ding, SH.H.,Guo, W.H.,Xu, N.Y.,Xiong, Y.ZH.,Jiang, S.W. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.7

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

Improvement of Magnetic Properties of SmCo<SUB>5</SUB>/α-Fe Nanocomposite Magnets by Magnetic Field Annealing

Y. Wei,Y. Q. Li,D. T. Zhang,M. Yue 한국자기학회 2018 Journal of Magnetics Vol.23 No.3

In this paper, magnetic field heat treatment was carried out for the SmCo5/α-Fe amorphous powders prepared by high-energy ball milling. The effects of annealing temperature and magnetic field on the crystallization, microstructure, and magnetic properties of the SmCo5/α-Fe nanocomposite permanent magnets were studied. The results show that the magnetic field can benefit the crystallization of SmCo5 phase and the degree of crystallinity of SmCo5/α-Fe alloy. Thus, the remanence and coercivity of the SmCo5/α-Fe magnets annealed with magnetic field are significant higher than those of the samples annealed without magnetic field. With the increase of annealing temperature in magnetic field heat treatment, the Sm(Co, Fe)5 phase and FeCo phase are gradually crystallized and grew up, while a small amount of Sm₂Co17 phase appears. The best magnetic properties are Mr = 73.29 emu/g, Mr/Ms = 0.59, and Hci = 6.20 kOe, when the annealing temperature is 700 ℃.

Synthesis of TiO₂ Nanotubes and Their Sensitivity for Toluene Gas

Yue, H.Y.,Huang, S.,Guo, E.J.,Wang, L.P.,Kang, F.W.,Yu, Z.M.,Guo, Y.K.,Sun, F.L. The Korean Powder Metallurgy Institute 2011 한국분말재료학회지 (KPMI) Vol.18 No.1

$TiO_2$ nanopowders with anatase structure were firstly prepared by controlling the pH value of a precursor solution without any heat-treatment at room temperature. The prepared $TiO_2$ nanopowders were hydrothermally treated in 10M NaOH solution at $170^{\circ}C$. Then, the samples were washed in DI water or 0.1M HCl. The $TiO_2$ nanotubes were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). The gas sensitivity of $TiO_2$ nanotubes for toluene gas was also investigated. The results show that $TiO_2$ nanotubes can be prepared by hydrothermal treatment. The morphology of $TiO_2$ nanotubes prepared by 0.1M HCl washing is destroyed to some extent. $TiO_2$ nanotubes with DI water washing show better sensitivity than that with 0.1M HCl washing.

The effect of second coagulant dose on the regrowth of flocs formed by charge neutralization and sweep coagulation using titanium tetrachloride (TiCl₄4)

Zhao, Y.X.,Gao, B.Y.,Shon, H.K.,Wang, Y.,Kim, J.H.,Yue, Q.Y. Elsevier Scientific Pub. Co 2011 Journal of hazardous materials Vol.198 No.-

Characteristics of flocs formed by charge neutralization and sweep coagulation using titanium tetrachloride (TiCl<SUB>4</SUB>) were investigated with humic acid-kaolin suspension by continuous optical monitoring. This paper focused on the regrowth ability of broken flocs after addition of second TiCl<SUB>4</SUB> dose. Variation of floc size and the fractal dimension of flocs versus second TiCl<SUB>4</SUB> dose after regrowth were investigated. Second TiCl<SUB>4</SUB> dose was added during the floc breakage period, and addition time of second TiCl<SUB>4</SUB> dose was also investigated. The results showed that, when coagulated by charge neutralization at pH 6, an appropriate second TiCl<SUB>4</SUB> dose improved regrowth ability of broken flocs at low initial TiCl<SUB>4</SUB> doses. While for high initial TiCl<SUB>4</SUB> doses, second TiCl<SUB>4</SUB> dose lowered floc re-growth ability. When coagulated by sweep coagulation at pH 10, second TiCl<SUB>4</SUB> dose made regrown flocs larger than those without second TiCl<SUB>4</SUB> dose. Floc structure analysis showed that it was determined by not only the fractal dimension of flocs, but also the chemical characteristics of floc surface. Addition time of second TiCl<SUB>4</SUB> dose had a great effect on floc regrowth ability, suggesting that the broken flocs had better regrowth when second TiCl<SUB>4</SUB> dose was added at the end of the breakage period.

ZnO Nanowire Arrays on 3D Hierachical Graphene Foam: Biomarker Detection of Parkinson’s Disease

Yue, Hong Yan,Huang, Shuo,Chang, Jian,Heo, Chaejeong,Yao, Fei,Adhikari, Subash,Gunes, Fethullah,Liu, Li Chun,Lee, Tae Hoon,Oh, Eung Seok,Li, Bing,Zhang, Jian Jiao,Huy, Ta Quang,Luan, Nguyen Van,Lee, Y American Chemical Society 2014 ACS NANO Vol.8 No.2

<P>We report that vertically aligned ZnO nanowire arrays (ZnO NWAs) were fabricated on 3D graphene foam (GF) and used to selectively detect uric acid (UA), dopamine (DA), and ascorbic acid (AA) by a differential pulse voltammetry method. The optimized ZnO NWA/GF electrode provided a high surface area and high selectivity with a detection limit of 1 nM for UA and DA. The high selectivity in the oxidation potential was explained by the gap difference between the lowest unoccupied and highest occupied molecular orbitals of a biomolecule for a set of given electrodes. This method was further used to detect UA levels in the serum of patients with Parkinson’s disease (PD). The UA level was 25% lower in PD patients than in healthy individuals. This finding strongly implies that UA can be used as a biomarker for PD.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2014/ancac3.2014.8.issue-2/nn405961p/production/images/medium/nn-2013-05961p_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn405961p'>ACS Electronic Supporting Info</A></P>

Effects of CSN1S2 Genotypes on Economic Traits in Chinese Dairy Goats

Yue, X.P.,Fang, Q.,Zhang, X.,Mao, C.C.,Lan, X.Y.,Chen, H.,Lei, Chuzhao Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.7

The aim of this study was to investigate allele frequencies at the CSN1S2 locus in two Chinese dairy goat breeds and the effects of its variation on dairy goat economic traits. Seven hundred and eight goats from Xinong Saanen (XS, n = 268) and Guanzhong (GZ, N = 440) breeds were selected. The milk samples of 268 XS goats were collected during the middle of lactation, body size parameters (708 goats) and daily milk yield (202 goats) were registered. The RFLP (restriction fragment length polymorphism) and SSCP (single strand conformation polymorphism) were used to detect the polymorphisms in CSN1S2. The Hardy-Weinberg (HW) equilibrium and the associations between body size, milk yield and composition and the genotypes were calculated. The results revealed that only A and F CSN1S2 alleles were found in the two Chinese dairy goat breeds. Allelic frequencies of A and F were 0.795, 0.205 and 0.739, 0.261 in Xinong Saanen and Guanzhong population respectively. Xinong Saanen breed was in Hardy-Weinberg equilibrium, while Guanzhong breed deviated from Hardy-Weinberg equilibrium (p<0.05). The association of polymorphism with economic traits indicated that the goats with FF genotype have higher milk fat and total solid concentration than those with AA and AF genotypes (p<0.05).

Edge contacts of graphene formed by using a controlled plasma treatment.

Yue, D W,Ra, C H,Liu, X C,Lee, D Y,Yoo, W J RSC Pub 2015 Nanoscale Vol.7 No.2

<P>Despite the fact that the outstanding properties of graphene are well known, the electrical performance of the material is limited by the contact resistance at the metal-graphene interface. In this study, we demonstrate the formation of 'edge-contacted' graphene through the use of a controlled plasma processing technique that generates a bond between the graphene edge and the contact metal. This technique controls the edge structure of the bond and significantly reduces the contact resistance. This simple approach requires no additional post-processing and has been proven to be very effective. In addition, controlled pre-plasma processing was applied in order to produce CVD-graphene field effect transistors with an enhanced adhesion and improved carrier mobility. The contact resistance attained by using pre-plasma processing was 270 ω μm, which is a decrease of 77%.</P>

The impact of pyriproxyfen on the development of honey bee (Apis mellifera L.) colony in field

Yue-Wen Chen,Pei-ShanWu,En-Cheng Yang,Yu-Shin Nai,Zachary Y. Huang 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3

Pyriproxyfen (PPN) is an insect growth regulator (IGR) that interferes with insect metamorphosis. Although the side effects of PPN on honey bee larval/adult stages have been studied, the risk to honey bee larvae from PPN residue in the environment is still unclear. In this study, we evaluated the impact of PPN on larval honey bees in field colonies by using an in vivo feeding assay. Oral toxicity to adult honey bees were determined. Finally, influence on royal jelly production was also examined. For in vivo feeding assay, the highest observed PPN treatment caused 67% mortality during pupal stage and in the remaining bees, 62.3% showed abnormal eclosion. Reductions in hatching rate, capping rate and adult emergence rate and increased abnormal eclosion rate were found in the colonies fed with 10 ppm PPN syrup. Oral toxicity test revealed that adult honey bees were less susceptible to PPN. Moreover, PPN reduced not only queen cell acceptance rate but also yield of royal jelly in queen cells. These results indicate that PPN has negative impacts on both larval and adult honey bees and royal jelly production, especially under high PPN concentrations. Since PPN is harmful to the development of honey bee larvae and pupae in the natural environment, the issue of honey bee colony contamination by PPN should be addressed.

Effect of α-Fe Content on the Magnetic Properties of MnBi/α-Fe Nanocomposite Permanent Magnets by Micro-magnetic Calculation

Y. Q. Li,M. Yue,J. H. Zuo,D. T. Zhang,W. Q. Liu,J. X. Zhang,Z. H. Guo,W. Li 한국자기학회 2013 Journal of Magnetics Vol.18 No.3

A finite element model was built for MnBi/α-Fe nanocomposite permanent magnets, and the demagnetization curves of the magnets were simulated by micro-magnetic calculation. The microstructure of the cubic model is composed of 64 irregular grains with an average grain size of 20 nm. With the volume fraction of soft magnetic phase (t vol. %) ranged from 5 to 20 vol. %, both isotropic and anisotropic nanocomposite magnets show typical single-phase permanent magnets behavior in their demagnetization curves, illustrating good intergranular exchange coupling effect between soft and hard magnetic phases. With the increase of volume fraction of soft magnetic phase in both isotropic and anisotropic magnets, the coercive force of the magnets decreases monotonically, while the remanence rises at first to a peak value, then decreases. The optimal values of maximum energy products of isotropic and anisotropic magnets are 84 and 200 kJ/m3, respectively. Our simulation shows that the MnBi/α-Fe nanocomposite permanent magnets own excellent magnetic properties and therefore good potential for practical applications.

Gas hydrates phase equilibria for structure I and II hydrates with chloride salts at high salt concentrations and up to 200MPa

Hu, Yue,Makogon, Taras Y.,Karanjkar, Prasad,Lee, Kun-Hong,Lee, Bo Ram,Sum, Amadeu K. Elsevier 2018 The Journal of chemical thermodynamics Vol.117 No.-

<P><B>Abstract</B></P> <P>Gas hydrates phase equilibria for structure I and II hydrates with chloride salts (NaCl, CaCl<SUB>2</SUB>, KCl and MgCl<SUB>2</SUB>) were measured at high salt concentrations and up to 200MPa. The measured equilibrium data represent three-phase (Solution – Hydrate – Vapor) or four-phase (Solution – Hydrate – Salt precipitated – Vapor) equilibrium depending on the salt concentration. The hydrate phase boundary with salts was shifted to lower temperatures and higher pressures when the experimental system was below the salt saturation concentration, while the boundaries were unchanged at salt concentrations above saturation, corresponding to quadruple points. The experimental data were compared with hydrate equilibrium predictions calculated by commonly used predictive tools to assess the reliability of these tools for the brines and conditions considered. The comparison demonstrates that predictive tools exhibit large deviation to the measured data, especially at high pressures and high salinity conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Gas hydrates phase equilibria with chloride salts were measured up to 200MPa. </LI> <LI> Predictions deviate from measured data at high salt concentration and high pressure. </LI> <LI> Measured data are valuable to test and improve hydrate predictive tools. </LI> </UL> </P>