Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

H.C. Liu,J.F. Huang,S.R. Lee,H.L. Liu,C.H. Hsieh,C.W. Huang,M.C. Huang,C. Tai,J.P. Poivey,R. Rouvier,Y.S. Cheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.5

A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie (h2 = 0.07±0.03) and M (h2 = 0.07±0.01), moderately low for Dm (h2 = 0.13±0.02), of medium values for H (h2 = 0.20±0.03) and F (h2 = 0.23±0.03). High and favourable genetic correlations existed between F and Dm (rg = 0.93), between F and H (rg = 0.97) and between Dm and H (rg = 0.90). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings’ feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

DETERMINATION OF THE APPARENT ILEAL DIGESTIBILITY OF PROTEIN AND AMINO ACIDS IN FEEDSTUFFS AND MIXED DIETS FOR GROWIG-FINSHING PIGS WITH THE MOBILE NYLON BAG TECHNIQUE

Yin, Y.L.,Zhong, H.Y.,Huang, R.L. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.5

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

SEARCH FOR PULSED γ-RAY EMISSION FROM GLOBULAR CLUSTER M28

Wu, J. H. K.,Hui, C. Y.,Wu, E. M. H.,Kong, A. K. H.,Huang, R. H. H.,Tam, P. H. T.,Takata, J.,Cheng, K. S. IOP Publishing 2013 ASTROPHYSICAL JOURNAL LETTERS - Vol.765 No.2

<P>Using the data from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope, we have searched for gamma-ray pulsations from the direction of the globular cluster M28 (NGC 6626). We report the discovery of a signal with a frequency consistent with that of the energetic millisecond pulsar (MSP) PSR B1821-24 in M28. A weighted H-test test statistic of 28.8 is attained, which corresponds to a chance probability of similar to 10(-5) (4.3 sigma detection). With a phase-resolved analysis, the pulsed component is found to contribute similar to 25% of the total observed gamma-ray emission from the cluster. However, the unpulsed level provides a constraint for the underlying MSP population and the fundamental plane relations for the scenario of inverse Compton scattering. Follow-up timing observations in radio/X-ray are encouraged to further investigate this periodic signal candidate.</P>

Selection for Duration of Fertility and Mule Duck White Plumage Colour in a Synthetic Strain of Ducks (Anas platyrhynchos)

Liu, H.C.,Huang, J.F.,Lee, S.R.,Liu, H.L.,Hsieh, C.H.,Huang, C.W.,Huang, M.C.,Tai, C.,Poivey, J.P.,Rouvier, R.,Cheng, Y.S. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.5

A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie ($h^2=0.07{\pm}0.03$) and M ($h^2=0.07{\pm}0.01$), moderately low for Dm ($h^2=0.13{\pm}0.02$), of medium values for H ($h^2=0.20{\pm}0.03$) and F ($h^2=0.23{\pm}0.03$). High and favourable genetic correlations existed between F and Dm ($r_g=0.93$), between F and H ($r_g=0.97$) and between Dm and H ($r_g=0.90$). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings' feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

차세대 가속기용 공동형 빔위치 측정기 개발

Kim S.H.,Park Y.J.,Hwang W.H.,Huang J.Y.,Honda Y.,Inoue Y. 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.4

국제 직선형 충돌 가속기 (ILC; International Linear Collider). 자유전자 레이저 (FEL: Free Electron Laser)와 같은 차세대 가속기에 사용 할 공동형 빔위치 측정기 ( 공동형 BPM: cavity-type beam position monitor)를 일본 고에너지 연구소 (KEK; High Energy Accelerator Research Organization)와 공동으로 개발하였다. ILC 및 FEL의 운전을 위해서는 빔 기반 정렬 (beam-based alignment)과 되먹임 장치 (feedback system)가 필수적으로 요구되는데, 이를 위해서는 적절한 위치에 서브마이크론의 분해능을 지닌 빔위치 측정기를 설치하여야 한다 [1]. 공동형 BPM은 기계적인 정밀도에 매우 민감하므로 정밀한 제작과 미세한 기계적 조정을 통하여 성능을 달성하게 된다. 우리는 제작 오차를 줄이기 위하여 공진 공동, 빔 튜브, 도파관, 전기도입기 등 모든 부품을 조립 후 한꺼번에 진공 브레이징 하였다. 공동의 외주면에는 네 개의 튜닝 핀을 두어 공진주파수 및 x-y 격리도 (x-y isolation between coupled waveguide)를 미세 조정할 수 있도록 하였다. 현재 개발된 공동형 BPM 은 공진주파수는 6.422 GHz 이며, 공동 내경은 53.822 mm, 빔의 위치 측정 범위는 ${\pm}250 {\mu}m$이다. network analyzer를 관측하면서 튜닝핀을 이용하여 x-y 격리도를 -40 dB 이하로 조정할 수 있었다. 실제 KEK ATF2에서의 전자빔을 이용한 시험에서 신호의 모양, x-y 격리도, 민감도 등에서 만족한 결과를 얻었다. Cavity-type beam position monitors were developed in collaboration with KEK to use for the future accelerators such as international linear collider (ILC) or x-ray free electron laser (XFEL) in PAL. BPM components such as BPM cavity, beam tubes, waveguides and feedthroughs were assembled by brazing at the same time to reduce mechanical errors during the fabrication. There are four screwed pins around outer rim of the cavity for the tuning of cavity frequency and x-y isolation. The resonance frequency of BPM is 6.422 GHz, the inner diameter of cavity is 53.822 mm, and the range of mechanical adjusting is $+ / - 250{\mu}m$. The x-y isolation was measured better than -40 dB after tuned. Test results of signal forms, x-y isolations, sensitivities are satisfied within requirements for the KEK ATF2 beam line.

Understanding spin configuration in the geometrically frustrated magnet TbB4: A resonant soft X-ray scattering study

H. Huang,H. Jang,B.Y. Kang,B.K. Cho,C.-C. Kao,Y.-J. Liu,J.-S. Lee 한국물리학회 2018 Current Applied Physics Vol.18 No.11

The frustrated magnet has been regarded as a system that could be a promising host material for the quantum spin liquid (QSL). However, it is difficult to determine the spin configuration and the corresponding mechanism in this system, because of its geometrical frustration (i.e., crystal structure and symmetry). Herein, we systematically investigate one of the geometrically frustrated magnets, the TbB4 compound. Using resonant soft x-ray scattering (RSXS), we explored its spin configuration, as well as Tb's quadrupole. Comprehensive evaluations of the temperature and photon energy/polarization dependences of the RSXS signals reveal the mechanism of spin reorientation upon cooling down, which is the sophisticated interplay between the Tb spin and the crystal symmetry rather than its orbit (quadrupole). Our results and their implications would further shed a light on the search for possible realization of QSL.

Effects of catalpalactone on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells

Huang, H.S.,Han, X.H.,Hwang, B.Y.,Park, J.I.,Yoo, S.K.,Lee, H.J.,Lim, S.C.,Lee, M.K. Elsevier Science B.V 2008 Environmental toxicology and pharmacology Vol.26 No.1

The effects of catalpalactone on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells were investigated. Catalpalactone at 5-30μM decreased intracellular dopamine content with the IC<SUB>50</SUB> value of 22.1μM. Catalpalactone at 5-20μM, but not 30μM, did not alter cell viability. Catalpalactone at 20μM inhibited tyrosine hydroxylase (TH) and aromatic-l-amino acid decarboxylase (AADC) activities. Catalpalactone also decreased cyclic AMP levels and inhibited TH phosphorylation. In addition, catalpalactone at 20μM reduced the increases in dopamine levels induced by L-DOPA (20-50μM). Catalpalactone (5-30μM) associated with L-DOPA (50-100μM) enhanced L-DOPA-induced cytotoxicity at 48h, which was prevented by N-acetyl-l-cysteine. These results suggest that catalpalactone inhibited dopamine biosynthesis by reducing TH and AADC activities and enhanced L-DOPA-induced cytotoxiciy in PC12 cells.

Understanding spin configuration in the geometrically frustrated magnet TbB₄: A resonant soft X-ray scattering study

Huang, H.,Jang, H.,Kang, B.Y.,Cho, B.K.,Kao, C.-C.,Liu, Y.-J.,Lee, J.-S. Elsevier 2018 Current Applied Physics Vol.18 No.11

<P>The frustrated magnet has been regarded as a system that could be a promising host material for the quantum spin liquid (QSL). However, it is difficult to determine the spin configuration and the corresponding mechanism in this system, because of its geometrical frustration (i.e., crystal structure and symmetry). Herein, we systematically investigate one of the geometrically frustrated magnets, the TbB4 compound. Using resonant soft x-ray scattering (RSXS), we explored its spin configuration, as well as Tb's quadrupole. Comprehensive evaluations of the temperature and photon energy/polarization dependences of the RSXS signals reveal the mechanism of spin reorientation upon cooling down, which is the sophisticated interplay between the Tb spin and the crystal symmetry rather than its orbit (quadrupole). Our results and their implications would further shed a light on the search for possible realization of QSL.</P>

A novel evidence theory model and combination rule for reliability estimation of structures

Y.R. Tao,Q. Wang,L. Cao,S.Y. Duan,Z.H.H. Huang,G.Q. Cheng 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.62 No.4

Due to the discontinuous nature of uncertainty quantification in conventional evidence theory(ET), the computational cost of reliability analysis based on ET model is very high. A novel ET model based on fuzzy distribution and the corresponding combination rule to synthesize the judgments of experts are put forward in this paper. The intersection and union of membership functions are defined as belief and plausible membership function respectively, and the Murfhy’s average combination rule is adopted to combine the basic probability assignment for focal elements. Then the combined membership functions are transformed to the equivalent probability density function by a normalizing factor. Finally, a reliability analysis procedure for structures with the mixture of epistemic and aleatory uncertainties is presented, in which the equivalent normalization method is adopted to solve the upper and lower bound of reliability. The effectiveness of the procedure is demonstrated by a numerical example and an engineering example. The results also show that the reliability interval calculated by the suggested method is almost identical to that solved by conventional method. Moreover, the results indicate that the computational cost of the suggested procedure is much less than that of conventional method. The suggested ET model provides a new way to flexibly represent epistemic uncertainty, and provides an efficiency method to estimate the reliability of structures with the mixture of epistemic and aleatory uncertainties.

East Asian GRB Follow-up Network (EAFON)

Y. Urata,K.Y. Huang,W.H. Ip,P.H. Kuo,Y.S Lee,Y. Qiu,J. Wei,J. Deng,W. Zheng,M. Zhai,M. Tashiro,K. Abe,K. Onda,T. Tamagawa,K. Ioka,M. Kuwahara,F. Usui 한국천문학회 2006 天文學會報 Vol.31 No.1