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Kang, S.,Kim, S.j.,Kim, S.H.,Lee, J.H.,Park, S.Y.,Ha, S.D. Published on behalf of the Canadian Institute of F 2016 Food Research International Vol.84 No.-
<P>Enteric noroviruses are occasionally detected in Kimchi, which is a traditional dish made of fermented vegetables. This study was aimed at examining the effects of two levels of salt concentrations on the survival of murine norovirus-1 (MNV-1), a human norovirus surrogate, in experimentally contaminated cabbage Kimchi stored at 5 degrees C for 10 weeks. The number of total aerobic bacteria (TAB) and lactic acid bacteria (LAB), MNV-1 titer pH, and acidity were measured every week The titers of MNV-1 in both low (1.17%) and normal (222%) salinity cabbage Kimchi were significantly (P < 0.05) decreased with increase in storage time. The overall reduction was 1.75 log(10) plaque-forming unit (PFU)/mL in normal salinity cabbage Kimchi and 1.24 log(10) PFU/mL in low salinity cabbage Kimchi. The time required to reduce the titer by >1 log(10) PFU/mL in normal and low salinity cabbage Kimchi were 4 and 8 weeks, respectively. The pH value under both salinities significantly (P < 0.05) decreased until 4 weeks. The maximum acidity was 0.83% and 0.79% in normal and low salinity cabbage Kimchi, respectively, during the 10 weeks. The population of TAB and LAB reached up to 733 log(10) colony-forming unit (CFU)/g as a maximum population during the storage period of 3 weeks in normal salinity cabbage Kimchi. However, the population of TAB and LAB in low salinity cabbage Kimchi reached to 6.99 and 7.04 log(10) CFU/g at 5 and 4 weeks, respectively. Through these findings, fermentation factors such as TAB, LAB, pH, and acidity of cabbage Kimchi were influenced by salt concentration. The inactivation of MNV-1 in normal salinity cabbage Kimchi was much faster than that in low salinity cabbage Kimchi because the fermentation in normal salinity cabbage Kimchi progressed more quickly than that in low salinity cabbage Kimchi. However, both salinity cabbage Kimchi were able to infect cells for 70 days even though the MNV-1 was reduced over 1 log(10) during fermentation. Therefore, the way to protect cabbage Kimchi from norovirus must be considered. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Kim, D.S.,Kim, M.J.,Cha, S.H.,Kim, H.M.,Kim, J.H.,Kim, K.N.,Lee, J.S.,Choi, J.Y.,Castells, V.B.,Kim, H.S.,Bang, J.,Oster, P. Decker 2016 INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES Vol.45 No.-
<P>Objectives: To assess the safety and immunogenicity of a meningococcal polysaccharide diphtheria toxoid conjugate vaccine (MenACYW-D) in a Korean population. Methods: This was a phase III, blind-observer, controlled study in which participants aged 11-55 years were randomized (2: 1 ratio) to a single dose of MenACYW-D or tetanus/diphtheria/acellular pertussis (Tdap) vaccine. Outcomes included rates of seroconversion against all serogroups (>= 4-fold increase in antibody titer from pre-vaccination), geometric mean titers (GMTs) at days 0 and 28 based on a serum bactericidal assay using baby rabbit complement, rates of seroprotection (titer >= 1: 128) at day 28, and safety. Results: A total of 300 participants were enrolled in the study (200 MenACYW-D and 100 Tdap). Seroconversion rates for serogroups A, C, Y, and W-135 were 77.8%, 88.3%, 74.6%, and 92.4%, respectively, for the MenACYW-D group and 9.3%, 8.1%, 12.2%, and 8.2%, respectively, for the Tdap group. The proportions of participants with pre-vaccination titers >= 1: 128 were 57.3%, 12.6%, 51.5%, and 22.2% for serogroups A, C, Y, and W-135, respectively; post-vaccination rates were 98.5%, 89.4%, 96.0%, and 95.0% for the MenACYW-D group. A lower proportion of participants reported solicited reactions with MenACYW-D (46.2%) compared with Tdap (76.8%). Conclusion: A single dose of MenACYW-D was well tolerated and elicited a robust immune response in Korean adolescents and adults. (C) 2016 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).</P>
Foxp3 is a key downstream regulator of p53-mediated cellular senescence
Kim, J-E,Shin, J-S,Moon, J-H,Hong, S-W,Jung, D-J,Kim, J H,Hwang, I-Y,Shin, Y J,Gong, E-Y,Lee, D H,Kim, S-M,Lee, E Y,Kim, Y S,Kim, D,Hur, D,Kim, T W,Kim, K-p,Jin, D-H,Lee, W-J Macmillan Publishers Limited 2017 Oncogene Vol.36 No.2
<P>The downstream events and target genes of p53 in the process of senescence are not fully understood. Here, we report a novel function of the forkhead transcription factor Foxp3, which is a key player in mediating T-cell inhibitory functions, in p53-mediated cellular senescence. The overexpression of Foxp3 in mouse embryonic fibroblasts (MEFs) accelerates senescence, whereas Foxp3 knockdown leads to escape from p53-mediated senescence in p53-expressing MEFs. Consistent with these results, Foxp3 expression resulted in the induction of senescence in epithelial cancer cells, including MCF7 and HCT116 cells. Foxp3 overexpression also increased the intracellular levels of reactive oxygen species (ROS). The ROS inhibitor N-acetyl-L-cysteine rescued cells from Foxp3-expression-induced senescence. Furthermore, the elevated ROS levels that accompanied Foxp3 overexpression were paralleled by an increase in p21 expression. Knockdown of p21 in Foxp3-expressing MEFs abrogated the Foxp3-dependent increase in ROS levels, indicating that Foxp3 acts through the induction of p21 and the subsequent ROS elevation to trigger senescence. Collectively, these results suggest that Foxp3 is a downstream target of p53 that is sufficient to induce p21 expression, ROS production and p53-mediated senescence.</P>
Kim, K. W.,Adhikari, G.,Adhikari, P.,Choi, S.,Ha, C.,Hahn, I. S.,Jeon, E. J.,Joo, H. W.,Kang, W. G.,Kim, H. J.,Kim, N. Y.,Kim, S. K.,Kim, Y. D.,Kim, Y. H.,Lee, H. S.,Lee, M. H.,Leonard, D. S.,Oh, S. Y IEEE 2016 IEEE transactions on nuclear science Vol.63 No.2
<P>In order to investigate discrimination between nuclear recoil and electron recoil events for the KIMS-NaI dark matter search experiment, we measured the pulse shapes produced by neutrons and gamma rays in a NaI(Tl) crystal. Relatively good pulse shape discrimination (PSD) power due to high light output of recently developed crystals makes it possible to test whether the annual modulation signal observed by the DAMA/LIBRA experiment is caused by nuclear recoil events. We applied the PSD to underground data taken with a 9.15 kg low-background and high-light-output NaI(Tl) crystal for 134 days. Good agreement between underground data and electron recoil events was observed.</P>
200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구
김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-
고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.
Kim, S.-Y.,Choi, H.-B.,Yoon, H.-Y.,Choi, E.-J.,Cho, B.,Kim, H.-K.,Kim, Y.-J.,Kim, H.-J.,Min, C.-K.,Kim, D.-W.,Lee, J.-W.,Min, W.-S.,Kim, C.-C.,Kim, T.-G. Blackwell Publishing Ltd 2007 Tissue antigens Vol.69 No.suppl1
<P>Abstract</P><P>Interactions between killer cell immunoglobulin-like receptors (KIRs) and human leukocyte antigen class I ligands influence the development of the natural killer cell repertoire and the responses to infection, cancer, and allogeneic tissue. In this study, the association of KIR genes with acute graft-<I>vs</I>-host disease (GVHD) was investigated in 44 pairs of leukemia patients and their unrelated donors for hematopoietic stem cell transplantation (HSCT). Donors with more than 12 KIR genes showed significantly decreased frequencies of severe acute GVHD compared with donors with less than 11 KIR genes (<I>P</I> < 0.05). The distribution of KIR genotypes was not different between severe and mild acute GVHD in patients and donors, respectively. These results suggest that the number of KIR genes in donors could influence the occurrence of acute GVHD after unrelated HSCT.</P>
Kim, J-S,Kim, M-A,Kim, T M,Lee, S-H,Kim, D-W,Im, S-A,Kim, T-Y,Kim, W H,Yang, H-K,Heo, D S,Bang, Y-J,Lee, K-U,Choe, K-J,Kim, N K Nature Publishing Group 2009 The British journal of cancer Vol.100 No.5
<P>The aim of this study was to analyse the impact of epidermal growth factor receptor (<I>EGFR</I>), thymidylate synthase (<I>TS</I>), dihydropyrimidine dehydrogenase (<I>DPD</I>), thymidine phosphorylase (<I>TP</I>), aurora kinase (ARK) A/B, and excision repair cross-complementing gene 1 (<I>ERCC1</I>) on the efficacy of adjuvant chemotherapy with 5-fluorouracil and cisplatin (FP) after curative gastric resection. Normal and cancer tissue were separately obtained from gastrectomy samples of 153 patients with AJCC stage III–IV (M0) who subsequently treated with adjuvant FP chemotherapy. <I>TS</I>, <I>DPD</I>, <I>TP</I>, <I>ERCC1</I>, and ARK proteins were measured by immunohistochemistry (IHC). <I>EGFR</I> expression was investigated using a standardized IHC with the <I>EGFR</I> PharmDx assay. Amplification of <I>EGFR</I> gene was analysed using fluorescent <I>in situ</I> hybridisation (FISH). In multivariate analysis, stage, ratio of positive to removed lymph nodes, and <I>EGFR</I> expression were significant prognostic factors for overall survival. Patients with higher <I>EGFR</I> expression had better overall survival than those with lower expression (relative risk: 0.475 (95% confidence interval, 0.282–0.791, <I>P</I>=0.005). Low <I>EGFR</I> expression might be a predictive marker for relapse in curative resected stage III–IV (M0) gastric cancer patients who received adjuvant FP chemotherapy.</P>
Angular Dependence of Exchange Bias and Coercive Field in CoFe/MnIr Epitaxial Bilayers
Kim, D.Y.,Kim, C.G.,Kim, C.-O.,Shibata, M.,Tsunoda, M.,Takahashi, M.,Kim, D.Y.,Kim, C.G.,Kim, C.-O.,Shibata, M.,Tsunoda, M.,Takahashi, M. IEEE 2005 IEEE transactions on magnetics Vol.41 No.10
We have measured the hysteresis loop and torque curves at various applied magnetic field angle in CoFe/MnIr epitaxial bilayers on single crystal MgO
DJ-1 regulates mast cell activation and IgE-mediated allergic responses
Kim, D.K.,Kim, H.S.,Kim, A.R.,Kim, J.H.,Kim, B.,Noh, G.,Kim, H.S.,Beaven, M.A.,Kim, Y.M.,Choi, W.S. Mosby 2013 The Journal of allergy and clinical immunology Vol.131 No.6
Background: DJ-1 is an antioxidant protein known to reduce levels of reactive oxygen species (ROS), but its presence or function in mast cells and allergic diseases is unknown. Objectives: We sought to determine the role and mechanism of DJ-1 in allergic responses in vitro and in vivo. Methods: ROS and DJ-1 levels in serum or culture medium were measured with ELISA kits. The role of DJ-1 was evaluated in mast cell cultures and passive cutaneous anaphylaxis in normal or DJ-1 knockout (KO) mice. The mechanism of DJ-1 action was examined by using immunoblotting, immunoprecipitation, RT-PCR, and other molecular biological approaches. Results: Patients with atopic dermatitis had increased levels of ROS and diminished levels of DJ-1. DJ-1 KO mice exhibited enhanced passive cutaneous anaphylaxis and augmented ROS levels in sera and bone marrow-derived mast cells (BMMCs). Furthermore, antigen-induced degranulation and production of TNF-α and IL-4 were significantly amplified in DJ-1 KO and anti-DJ-1 small interfering RNA-transfected BMMCs compared with that seen in wild-type (WT) BMMCs. Studies with these cells and BMMCs transfected with small interfering RNAs against the phosphatases Src homology domain 2-containing protein tyrosine phosphatase (SHP) 1 and SHP-2 revealed that the DJ-1 KO phenotype could be attributed to suppression of SHP-1 activity and enhancement of SHP-2 activity, leading to strengthened signaling through linker for activation of T cells, phospholipase Cγ, and mitogen-activated protein kinases. Conclusions: A deficiency or constitutive activation of DJ-1 can have implications in mast cell-driven allergic diseases, such as asthma and anaphylaxis.