Single nucleotide polymorphism-based analysis of the genetic structure of Liangshan pig population

Liu, Bin,Shen, Linyuan,Guo, Zhixian,Gan, Mailing,Chen, Ying,Yang, Runling,Niu, Lili,Jiang, Dongmei,Zhong, Zhijun,Li, Xuewei,Zhang, Shunhua,Zhu, Li Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: To conserve and utilize the genetic resources of a traditional Chinese indigenous pig breed, Liangshan pig, we assessed the genetic diversity, genetic structure, and genetic distance in this study. Methods: We used 50K single nucleotide polymorphism (SNP) chip for SNP detection of 139 individuals in the Liangshan Pig Conservation Farm. Results: The genetically closed conserved population consisted of five overlapping generations, and the total effective content of the population (Ne) was 15. The whole population was divided into five boar families and one non-boar family. Among them, the effective size of each generation subpopulation continuously decreased. However, the proportion of polymorphic markers (P_N) first decreased and then increased. The average genetic distance of these 139 Liangshan pigs was 0.2823±0.0259, and the average genetic distance of the 14 boars was 0.2723±0.0384. Thus, it can be deduced that the genetic distance changed from generation to generation. In the conserved population, 983 runs of homozygosity (ROH) were detected, and the majority of ROH (80%) were within 100 Mb. The inbreeding coefficient calculated based on ROH showed an average value of 0.026 for the whole population. In addition, the inbreeding coefficient of each generation subpopulation initially increased and then decreased. In the pedigree of the whole conserved population, the error rate of paternal information was more than 11.35% while the maternal information was more than 2.13%. Conclusion: This molecular study of the population genetic structure of Liangshan pig showed loss of genetic diversity during the closed cross-generation reproduction process. It is necessary to improve the mating plan or introduce new outside blood to ensure long-term preservation of Liangshan pig.

Viscosity evaluation of Fe–Ni–Co ternary alloy from the measured binary systems

Yanhui Liu,Xuewei Lv,Chenguang Bai,Pingsheng Lai,JinSheng Wang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.30 No.-

The iso-viscosity curves of liquid Fe–Ni–Co ternary alloys at 1600 8C were investigated with consideringthe excess viscosity of ternary alloy by the means of three models (Kohler, Toop and Chou) from themeasured sub-binary data in this work. Excess viscosities were used instead of excess thermodynamicproperties in geometrical models, and the excess viscosities of the sub-binary systems were fit by usingthe 3rd degree (n = 3) Redlich–Kister polynomial. The increase of Ni content results in the decrease of theviscosity of the ternary alloy, while Fe has opposite effect. When the molar content of Ni within the alloydoes not exceed 50%, the increase of Co lowers the viscosity. Once its content is above 50%, Co canpromote the increase of the viscosity of the ternary alloy. Similarity coefficients of the Fe–Ni, Fe–Co andNi–Co three binary systems mentioned in the Chou model have been calculated and their values are 0.82,0.20 and 0.47, respectively, showing that the Fe–Ni–Co ternary system is not exactly the ‘‘Kohler model’’or ‘‘Toop model’’, so both Kohler model and Toop model cannot obtain the accurate predicted values. Thepredicted iso-viscosity curves calculated by the Chou model should be recommended. Some activationenergies of the ternary alloy were calculated by the three models and compared with three sub-binarysystems, and the evaluated activation energies calculated by the Chou model should be recommended. Except the sample AE3 and AE6, other activation energy values predicted by three different models aresimilar by a narrow margin. The activation energies for AE3 and AE6 are the lowest and highest,respectively, because of the mix effect of the ternary alloy.

Study on the Influence of Dynamic Characteristics of Servo Valve on Coupling Vibration of Cold Rolling Mill

Yujie Liu,Shen Wang,Jiebin Qi,Xuewei Wang,Xiaoqiang Yan 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.8

The cold rolling mills are complex electro-hydro-mechanical systems in which vibrations are usually detected as issues during production process. In the present work, the vibrations with frequencies of 80Hz were found at top of the rolling mill and the pressure behind servo valve during the production process. In order to understand the vibration mechanism and to explore the measurements for vibration suppression, the dynamic models were proposed to describe valve spool, 8-DOF cold rolling mill, and hydraulic system based on the dynamic characteristics of servo-valve. The analytical solutions and numerical solutions were obtained for the vibration rules for the valve spool displacement and the output flow rate under different working conditions. Moreover, the influence of dynamic characteristics of servo-valve on the vibrations and optimization of the servo-valve were discussed. The results suggest that the dynamic characteristics of servo-valve have a significant influence on the hydro-mechanical coupled vibration of rolling mill and the vibrations can be dampened by improving the dynamic characteristics of rolling mill. This work is expected to provide a new perspective to suppress the hydro-mechanical coupled vibrations.

Long-chain alcohols derived from the microalga Monoraphidium

Yang, Xuewei,Dai, Xin,Zhang, Rui,Shao, Cong,Geng, Shu,Chen, Guangyi,Liu, Xianhua,Wang, Guangyi Techno-Press 2013 Advances in energy research Vol.1 No.2

This study was to investigate the composition and characteristics of long-chained alcohols extracted from the algal strain Monoraphidium 3s35. The production of biomass was optimized using different cultivation methods. Under the aerated growth condition, this strain yielded up to 37.26% extracts of dry weight and $576mgL^{-1}$ biomass. The major compounds of the extracts are mainly long-chained alcohols (89.24%), with carbon chain length ranging from 12 to 20. Interestingly, or the long-chained alcohols, 3-(2-Methoxyethyl)-1-nonanol, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol and oleyl alcohol accounted for 53.68%, 23.45%, and 12.11%, respectively. Because of their amphipathic nature, these long-chained alcohols have been widely used in bioenergy production and cosmetics industry. Furthermore, Monoraphidium 3s35 produced 9.73% of $C_{17}$ and $C_{20}$ alkanes, which can be used as an important supplement for the petrodiesel-like fuel.

Identifying gas hydrate occurrence state by seismic P-wave attenuation in northern South China Sea

Danqing Dai,Xuewei Liu,Chuanhui Li 한국지질과학협의회 2018 Geosciences Journal Vol.22 No.3

In the process of gas hydrates exploration, the gas hydrate occurrence state is the key factor to determine the exploiting method. In this paper, we find a way to identify gas hydrate occurrence states by seismic P-wave attenuation. We find that the P-wave attenuation characteristics are different between the pore-filling hydrate-bearing sediments and the fracture-filling hydratebearing sediments. The attenuation is prominent in fracture-filling hydrate-bearing sediments. However, the pore-filling hydratebearing sediments have week attenuation effect. We use this difference to identify the gas hydrate occurrences state and apply it in northern South China Sea. The results depict that the P-wave attenuation is an effective attribute to identify gas hydrate occurrence states, which lays a foundation for the identification of gas hydrate occurrence state by seismic P-wave attenuation.

Cotton GhKCH2, a Plant-specific Kinesin, is Low-affinitive and Nucleotide-independent as Binding to Microtubule

Xu, Tao,Sun, Xuewei,Jiang, Shiling,Ren, Dongtao,Liu, Guoqin Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Kinesin is an ATP-driven microtubule motor protein that plays important roles in control of microtubule dynamics, intracellular transport, cell division and signal transduction. The kinesin superfamily is composed of numerous members that are classified into 14 subfamilies. Animal kinesins have been well characterized. In contrast, plant kinesins have not yet to be characterized adequately. Here, a novel plant-specific kinesin gene, GhKCH2, has been cloned from cotton (Gossypium hirsutum) fibers and biochemically identified by prokaryotic expression, affinity purification, ATPase activity assay and microtubule-binding analysis. The putative motor domain of GhKCH2, $M_{396-734}$ corresponding to amino acids Q396-N734 was fused with 6$\times$His-tag, soluble-expressed in E. coli and affinity-purified in a large amount. The biochemical analysis demonstrated that the basal ATPase activity of $M_{396-734}$ is not activated by $Ca^{2+}$, but stimulated 30-fold max by microtubules. The enzymatic activation is microtubule-concentration-dependent, and the concentration of microtubules that corresponds to half-maximum activation was about 11 ${\mu}M$, much higher than that of other kinesins reported. The cosedimentation assay indicated that $M_{396-734}$ could bind to microtubules in vitro whenever the nucleotide AMP-PNP is present or absent. As a plant-specific microtubule-dependent kinesin with a lower microtubule-affinity and a nucleotide-independent microtubule-binding ability, cotton GhKCH2 might be involved in the function of microtubules during the deposition of cellulose microfibrils in fibers or the formation of cell wall.

Experimental study on deformation of concrete for shotcrete use in high geothermal tunnel environments

Shengai Cui,Pin Liu,Xuewei Wang,Yibin Cao,Yuezhong Ye 사단법인 한국계산역학회 2017 Computers and Concrete, An International Journal Vol.19 No.5

Taking high geothermal tunnels as background, the deformation of concrete for shotcrete use was studied by simulating hot-humid and hot-dry environments in a laboratory. The research is made up by two parts, one is the influence of two kinds of high geothermal environments on the deformation of shotcrete, and the other is the shrinkage inhibited effect of fiber materials (steel fibers, polypropylene fibers, and the mixture of both) on the concrete in hot-dry environments. The research results show that: (1) in hot and humid environments, wet expansion and thermal expansion happened on concrete, but the deformation is smooth throughout the whole curing age. (2) In hot and dry environments, the concrete suffers from shrinkage. The deformation obeys linear relationship with the natural logarithm of curing age in the first 28 days, and it becomes stable after the 28th day. (3) The shrinkage of concrete in a hot and dry environment can be inhibited by adding fiber materials especially steel fibers, and it also obeys linear relationship with the natural logarithm of curing age before it becomes stable. However, compared with no-fiber condition, it takes 14 days, half of 28 days, to make the shrinkage become stable, and the shrinkage ratio of concrete at 180-day age decreases by 63.2% as well. (4) According to submicroscopic and microscopic analysis, there is great bond strength at the interface between steel fiber and concrete. The fiber meshes are formed in concrete by disorderly distributed fibers, which not only can effectively restrain the shrinkage, but also prevent the micro and macro cracks from extending.

Genetic parameter estimation for reproductive traits in QingYu pigs and comparison of carcass and meat quality traits to Berkshire×QingYu crossbred pigs

Luo Jia,Yang Yiting,Liao Kun,Liu Bin,Chen Ying,Shen Linyuan,Chen Lei,Jiang An’an,Liu Yihui,Li Qiang,Wang Jinyong,Li Xuewei,Zhang Shunhua,Zhu Li 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.8

Objective: The QingYu pig is well known for its excellent meat quality attributes in Sichuan province, China. In order to improve its production efficiency, the determination of genetic factors contributing to quantifiable economic traits of livestock is important. Moreover, the cross-breeding of QingYu pigs with western breeds possessing strong growth attributes is an efficient way to improve the performance of this breed. Methods: Here, the genetic parameters of several important reproductive traits of QingYu pigs were estimated, include total number born (TNB), number born alive, litter birth weight, individual birth weight, number of piglets weaned, litter weaning weight, and individual weaning weight. The data was analyzed using the ASReml 3.0 software (NSW Inc., Sydney, Australia). Furthermore, the effects of crossing Berkshire with QingYu (BQ) pigs on carcass and meat quality traits, as well as the effects of slaughter weight on carcass and meat quality of BQ were characterized. Results: QingYu pigs exhibited superior reproductive traits. The TNB available to QingYu pigs was more than 8 per parity. The observed repeatability of the reproductive traits of the QingYu pigs was between 0.10 and 0.23. The significantly correlated genetic and phenotypic of reproduction traits were consistent. Interestingly, the BQ pigs exhibited improved carcass quality, with a significant increase in loin muscle area, lean percentage and reduction in sebum percentage. As a result, BQ had higher L45min, lower cooking scores, and lower drip loss. In addition, the loin muscle area, body length, and sebum percentage were significantly higher in 90 and 100 kg animals. Cooking loss showed a significant increase at 80 kg, and marbling increased significantly from 90 kg. Conclusion: The results of this study suggest that QingYu pigs exhibit excellent reproductive properties and heritability of these traits. Crossing with Berkshire is an efficient strategy to improve the carcass and meat quality of QingYu pigs for commercial operations. Furthermore, it appears as though the optimal slaughter weight of BQ pigs is at approximately 90 kg.

Genome-wide analysis and environmental response profiling of dirigent family genes in rice (Oryza sativa)

Rongjun Chen,Yongrong Liao,Shengbin Liu,Yunyun Jiang,Changqiong Hu,Xuewei Zhang,Xufeng Cao,Zhengjun Xu,Xiaoling Gao,Lihua Li,Jianqing Zhu 한국유전학회 2017 Genes & Genomics Vol.39 No.1

Dirigent (DIR) and DIR-like family genes were involved in lignification or in the response to pathogen infection and abiotic stress in plants. Little is known to us about how rice DIR genes respond to adverse conditions. In this study, we reported genome-wide analysis of 49 DIR or DIR-likes genes in rice. The 49 OsDIRs or OsDIR-likes were tandem arranged into ten clusters. The phylogenetic analysis indicated that the 49 rice DIR and DIR-like genes cluster into five distinct subfamilies, DIR-a and four DIRlike subfamilies (DIR-b/d, and DIR-g, DIR-c, DIR-e). Meta-analysis of microarray gene expression datas indicated that all the OsDIRs or OsDIR-likes were expressed almost at the same level but with different patterns: most OsDIRs or OsDIR-likes were expressed exclusively in stigma and ovary and were induced by IAA and BAP; several genes were induced by trans-zeatin (tZ) and DMSO; 23 OsDIRs or OsDIR-likes were responded to abiotic stress. Our analysis also showed that most of these genes could respond to abiotic stresses, which contained cis-regulatory elements. The present study will provide a useful reference for further functional analysis of the DIR genes in rice.

Anaerobic Ammonium-Oxidizing Bacteria in Cow Manure Composting

( Tingting Wang ),( Lijun Cheng ),( Wenhao Zhang ),( Xiuhong Xu ),( Qingxin Meng ),( Xuewei Sun ),( Huajing Liu ),( Hongtao Li ),( Yu Sun ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7

Composting is widely used to transform waste into valuable agricultural organic fertilizer. Anaerobic ammonium-oxidizing (anammox) bacteria play an important role in the global nitrogen cycle, but their role in composting remains poorly understood. In the present study, the community structure, diversity, and abundance of anammox bacteria were analyzed using cloning and sequencing methods by targeting the 16S rRNA gene and the hydrazine oxidase gene (hzo) in samples isolated from compost produced from cow manure and rice straw. A total of 25 operational taxonomic units were classified based on 16S rRNA gene clone libraries, and 14 operational taxonomic units were classified based on hzo gene clone libraries. The phylogenetic tree analysis of the 16S rRNA gene and deduced HZO protein sequences from the corresponding encoding genes indicated that the majority of the obtained clones were related to the known anammox bacteria Candidatus “Brocadia,” Candidatus “Kuenenia,” and Candidatus “Scalindua.” The abundances of anammox bacteria were determined by quantitative PCR, and between 2.13 × 10⁵ and 1.15 × 10⁶ 16S rRNA gene copies per gram of compost were found. This study provides the first demonstration of the existence of anammox bacteria with limited diversity in cow manure composting.