RING Finger Proteins Are Involved in the Progression of Barrett Esophagus to Esophageal Adenocarcinoma: A Preliminary Study

( Xing Wei Wang ),( Wei Wei ),( Wei Qiang Wang ),( Xiao Yan Zhao ),( Hong Guo ),( Dian Chun Fang ) 대한소화기학회 2014 Gut and Liver Vol.8 No.5

Background/Aims: To investigate the differential expression of RING finger (RNF) proteins in Barrett esophagus (BE) and esophageal adenocarcinoma (EAC). Methods: The differential expression of RNFs in normal esophagus (NE), BE, and EAC was screened using microarray assay. Real-time quantitative polymerase chain reaction (PCR), tissue microarray assay, and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs. Results: The expression of nine RNFs in the BE or EAC was 2-fold higher than those in NE. Among these proteins, the RNF32 and RNF121 expression in BE was 20.3-fold and 16.4-fold higher, respectively, than that in NE, and the expression of RNF24, RNF130, RNF141, RNF139, RNF11, RNF14, and RNF159 was upregulated more than 2-fold compared with NE. The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE. The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE. Furthermore, the mRNA expression of all RNFs, except for RNF141 in EAC, was dramatically higher than those in the BE. Similar results were also obtained from the Western blot analysis. Conclusions: A total of nine RNFs play critical roles in the progression of BE to EAC. (Gut Liver 2014;8:487-494)

The Crystal Structure of One Natural Compound Cyclo-(1,10-Docandiamino-11,20-Docanedioic) Amide (1,12-Diazacyclodocosane-2,11-Dione)

Wei, Wan-Xing,Pan, Yuan-Jiang Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.11

1,12-diazacyclodocosane-2,11-dione was first isolated from a plant Phyllanthus niruri Linn. Its structure has been determined by means of spectroscopy methods and X-ray crystallography. Two peptide groups in the big ring (lactam) are the main factors influencing intermolecular contacts. The hydrogen-bond interaction of these hydrophilic groups is observed in the crystal structure. Meanwhile, C-H···O hydrogen bonds in molecules contribute to the formation of the whole crystal. These two kinds of hydrogen-bond form six- member rings among molecules. This compound crystallizes in the triclinic space group P-1 with a= 9.588(1) $\AA$, b= $9.850(1)\AA$, c = $11.810(1)\AA$, $\alpha=$ 68.18(1)$^{\circ}C$ , $\beta=$ 84.98(1), $\gamma$ = 86.03(1)$^{\circ}C$ , V = $1030.66(17)\AA3$ , Z = 2. A disorder of five-member carbon chain in the whole ring is observed in the title compound. The bond angle 105.8(4) is determined for a extreme configuration C(14)-C(15)-C(16), and 117.7(10) for another extreme configuration C(14')-C(15')-C(16'). In this crystal, two molecules are tied each other by short intermolecular hydrogen bonds, the oxygen atom being tied by hydrogen bond to nitrogen atom of another two molecules. The NMR and IR spectral data coincides to the structure of the compound.

Recovery of metals from chloride leach solutions of anode slimes by solvent extraction. Part I: Recovery of gold with Cyanex 272

Xing, Wei Dong,Lee, Man Seung,Senanayake, Gamini Elsevier 2018 Hydrometallurgy Vol.180 No.-

<P><B>Abstract</B></P> <P>A separation process was developed to recover Au(III) from the chloride leach solution of anode slime using selective extraction and stripping step. The anode slime contained Ag, Au, Cu, Ni, Sn and Zn. The extraction with Cyanex 272 in kerosene was conducted by varying the HCl concentration of the solution from 0.5 to 9 M. Most of Au(III) and Sn(II) were extracted into Cyanex 272/kerosene phase together with a small amount of Cu(II) and Zn(II). The Au(III) in the loaded Cyanex 272 was selectively stripped over Sn(II) by (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>. The McCabe-Thiele diagrams for the extraction and stripping were constructed. Extra pure Au(III) solution was recovered from the leach liquor by batch simulation experiments of two stage countercurrent extraction with Cyanex 272 and by one stage stripping with (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>. The developed process can be applied to recover gold from a hydrochloric acid solution containing Ag(I), Cu(II), Sn(II) and Zn(II).</P> <P><B>Highlights</B></P> <P> <UL> <LI> A process was developed to recover Au(III) from anode slime leaching solution. </LI> <LI> HCl concentration was varied from 0.5 to 9 M to investigate the separation behavior. </LI> <LI> Cyanex 272 selectively extracted Au(III) and Sn(II) over Cu(II), Zn(II), Ni(II) and Ag(I). </LI> <LI> Only Au(III) in the loaded Cyanex 272 was stripped by (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>. </LI> <LI> The purity of Au(III) in stripping solution was higher than 99.9%. </LI> </UL> </P>

Dynamic Profiles of Ubiquitination and Autophagy Associated with Paternal Mitochondria Degradation during Mouse Postfertilization Development

Xing-Wei Liang,Yong-Xun Jin,Ga-Young An,Seul-Ki Lee,Jung-Woo Kwon,Xiang-Shun Cui,Nam-Hyung Kim 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s

It is well established that mitochondrial genome is strictly maternally inherited in mammalian, despite the fact that paternal mitochondria enter into oocyte during fertilization. To date, although some mechanisms have been extrapolated to interpret the elimination of paternal mitochondria, the exact mechanism still is unclear. Recent studies suggest that autophagy process and the ubiquitin-mediated degradation pathway may be involved in elimination of paternal mitochondria. However, the dynamic profiles of autophagy and ubiquitination associated with paternal mitochondria degradation have not been determined in mouse model. Through immunostaining with specific antibody LC3 and Ubiquitin and confocal microscopy, we investigated the dynamic profiles of LC3 and Ubiquitin signals in mouse embryos during preimplantation development. In addition, embryos were stained with MitoTracker Red for tracking the degradation process of paternal mitochondria. Our results showed that paternal mitochondria gradually degraded during postfertilization development, and sporadic paternal mitochondria were found at least in 16 cell embryos. LC3 and Ubiquitin signals appeared in the midpiece of sperm at 3 h postfertilization, and they were strictly colocalizated with paternal mitochondria from zygote to 2 cell embryo. Nevertheless, the colocalization became loose at 4 cell embryos, and gradually disappeared beyond 4 cell embryos. Our results confirmed that autophagy process and the ubiquitin-mediated degradation pathway may take part in the postfertilization remove of paternal mitochondria.

Tumor Promoting Function of DUSP10 in Non-Small Cell Lung Cancer Is Associated With Tumor-Promoting Cytokines

Xing Wei,Chin Wen Png,Madhushanee Weerasooriya,Heng Li,Chenchen Zhu,Guiping Chen,Chuan Xu,Yongliang Zhang,Xiaohong Xu 대한면역학회 2023 Immune Network Vol.23 No.4

Lung cancer, particularly non-small cell lung cancer (NSCLC) which contributes more than 80% to totally lung cancer cases, remains the leading cause of cancer death and the 5-year survival is less than 20%. Continuous understanding on the mechanisms underlying the pathogenesis of this disease and identification of biomarkers for therapeutic application and response to treatment will help to improve patient survival. Here we found that a molecule known as DUSP10 (also known as MAPK phosphatase 5) is oncogenic in NSCLC. Overexpression of DUSP10 in NSCLC cells resulted in reduced activation of ERK and JNK, but increased activation of p38, which was associated with increased cellular growth and migration. When inoculated in immunodeficient mice, the DUSP10-overexpression NSCLC cells formed larger tumors compared to control cells. The increased growth of DUSP10- overexpression NSCLC cells was associated with increased expression of tumor-promoting cytokines including IL-6 and TGFβ. Importantly, higher DUSP10 expression was associated with poorer prognosis of NSCLC patients. Therefore, DUSP10 could severe as a biomarker for NSCLC prognosis and could be a target for development of therapeutic method for lung cancer treatment.

Recovery of metals from chloride leach solutions of anode slimes by solvent extraction. Part II: Recovery of silver and copper with LIX 63 and Alamine 336

Xing, Wei Dong,Lee, Man Seung,Senanayake, Gamini Elsevier 2018 Hydrometallurgy Vol.180 No.-

<P><B>Abstract</B></P> <P>In Part 1 of this paper, gold(III) and Sn(II) were recovered from the chloride leach solution of anode slimes by extraction with Cyanex 272 and stripping with (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB> and NaOH solutions for gold(III) and Sn(II), sequentially. In this work, solvent extraction experiments were conducted to recover Ag(I) and Cu(II) from the Au(III) free raffinate containing Ni(II), Sn(II) and Zn(II) using two systems. In the first system, Cu(II) and Ag(I) were sequentially separated using LIX 63 and Alamine 336, respectively. Dilute HCl and NH<SUB>4</SUB>SCN were used as the stripping agents for Cu(II) and Ag(I), respectively. In the second system, Cyanex 301 co-extracted Ag(I), Cu(II) and Sn(II) from the Au(III) free raffinate. Aqua regia at different dilutions stripped these three metal ions from the loaded Cyanex 301. First, Cu(II) was selectively stripped from the loaded Cyanex 301 by employing ten times diluted aqua regia. Second, most of Ag(I) was selectively stripped over Sn(II) by five times diluted aqua regia. A process flowsheet for the separation and recovery of noble and base metals from the chloride leach solution of anode slime using extraction and stripping has been proposed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Au(III), Ag(I) and Cu(II) were recovered from the leach liquor of anode slime. </LI> <LI> Gold(III) was first separated by selective extraction with Cyanex 272. </LI> <LI> Cu(II) was then recovered by extraction with by LIX 63 and stripping with HCl solution. </LI> <LI> Extraction with Alamine 336 followed by stripping with NH<SUB>4</SUB>SCN led to Ag(I) solution. </LI> <LI> Both Cu(II) and Ag(I) was simultaneously extracted by Cyanex 301. </LI> </UL> </P>

Separation of gold(III) from hydrochloric acid solution containing platinum(IV) and palladium(II) by solvent extraction with Cyanex 272 and LIX 63

Xing, Wei Dong,Lee, Man Seung,Kim, Young Hwan Elsevier 2018 Journal of industrial and engineering chemistry Vol.59 No.-

<P><B>Abstract</B></P> <P>Solvent extraction experiments were performed to separate Au(III), Pt(IV) and Pd(II) in the HCl concentration ranged from 0.5 to 9M. Cyanex 272 extracted only Au(III) among the three metals, while both Au(III) and Pd(II) was extracted by LIX 63 in the experimental ranges. The Au(III) in the loaded Cyanex 272 and LIX63 was stripped by either (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB> or (NH<SUB>2</SUB>)<SUB>2</SUB>CS. The concentration ratio of Au(III) to Pd(II) affected the extraction behavior of Au(III) and Pd(II) by LIX 63. The characteristic vibration bands of Cyanex 272 and LIX 63 were proposed by analyzing the FT-IR.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Solvent extraction experiments were done to separate Au(III), Pt(IV) and Pd(II). </LI> <LI> HCl concentration was varied from 0.5 to 9M to investigate the separation behavior. </LI> <LI> Cyanex 272 extracted only Au(III), leaving Pt(IV) and Pd(II) in the raffinate. </LI> <LI> LIX 63 extracted both Pd(II) and Au(III), leaving Pt(IV) in the raffinate. </LI> <LI> The Au(III) in the loaded Cyanex 272 and LIX63 was stripped by (NH<SUB>4</SUB>)<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB> and (NH<SUB>2</SUB>)<SUB>2</SUB>CS. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Effect of HCl concentration on the extraction percentage of metals. Experimental conditions: (a) [Cyanex 272]=0.2M, (b) [LIX 63]=0.2M; [Au]=[Pd]=[Pt]=100mgL<SUP>−1</SUP>, shaking time, 30min; O/A=1.</P> <P>[DISPLAY OMISSION]</P>

The K+/H+ antiporter AhNHX1 improved tobacco tolerance to NaCl stress by enhancing K+ retention

Wei-Wei Zhang,Jing-Jing Meng,Jin-Yi Xing,Sha Yang,Feng Guo,Xin-Guo Li,Shu-Bo Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.3

High salinity is the one of important factors limiting plant growth and crop production. Many NHX-type antiporters have been reported to catalyze K+/H+ exchange to mediate salt stress. This study shows that an NHX gene from Arachis hypogaea L. has an important role in K+ uptake and transport, which affects K+ accumulation and plant salt tolerance. When overexpressing AhNHX1, the growth of tobacco seedlings is improved with longer roots and a higher fresh weight than the wild type (WT) under NaCl treatment. Meanwhile, when exposed to NaCl stress, the transgenic seedlings had higher K+/H+ antiporter activity and their roots got more K+ uptake. NaCl stress could induce higher K+ accumulation in the roots, stems, and leaves of transgenic tobacco seedlings but not Na+ accumulation, thus, leading to a higher K+/Na+ ratio in the transgenic seedlings. Additionally, the AKT1, HAK1, SKOR, and KEA genes, which are involved in K+ uptake or transport, were induced by NaCl stress and kept higher expression levels in transgenic seedlings than in WT seedlings. The H+-ATPase and H+-PPase activities were also higher in transgenic seedlings than in the WT seedlings under NaCl stress. Simultaneously, overexpression of AhNHX1 increased the relative distribution of K+ in the aerial parts of the seedlings under NaCl stress. These results showed that AhNHX1 catalyzed the K+/H+ antiporter and enhanced tobacco tolerance to salt stress by increasing K+ uptake and transport.

Shear behavior of multi-hole perfobond connectors in steel-concrete structure

Xing Wei,Lin Xiao,Shiling Pei 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.56 No.6

This study focuses on the load carrying capacity and the force transfer mechanism of multi-hole perfobond shear connectors in steel-concrete composite structure. The behavior of multi-hole perfobond shear connector is more complicated than single-hole connector cases. 2 groups push-out tests were conducted. Based on the test results, behavior of the connection was analyzed and the failure mechanism was identified. Simplified iterative method and analytic solution were proposed based on force equilibrium for analyzing multi-hole perfobond shear connector performance. Finally, the sensitivity of design parameters of multi-hole perfobond shear connector was investigated. The results of this research showed that shear force distribution curve of multi-hole perfobond shear connector is near catenary. Shear forces distribution were determined by stiffness ratio of steel to concrete member, stiffness ratio of shear connector to steel member, and number of row. Efficiency coefficient was proposed to should be taking into account in different limit state.

New Lignan Isolated from Phyllanthus niruri Linn. Structure Elucidation by NMR Spectroscopy

Wei, Wan-Xing,Gong, Xing-guo,Ishrud, Omar,Pan, Yuan-Jiang Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.6