Study on the Reduction Properties of Thiourea Dioxide and Its Application in Discharge Printing of Polyester Fabrics

Xiaoyun Liu,Mengmeng Xie,Yanchao Li,Lan Zhou,Jianzhong Shao 한국섬유공학회 2018 Fibers and polymers Vol.19 No.6

The thiourea dioxide (TDO) was tentatively applied as a reductive discharging agent onto the discharge printing of polyester fabrics. The effect of aqueous solution temperature on the reducing capacity of TDO was thoroughly investigated by measuring oxidation-reduction potentials and hydrolysis kinetic curves. The effects of TDO on disperse dyes in aqueous solution and disperse dyes printed on the surfaces of glass substrates or polyester fabrics were investigated by analyzing the color changes before and after TDO discharge treatment. It was confirmed that TDO reduction became stronger with increasing aqueous solution temperature. TDO could destroy the chromogenic groups of the selected azo-dyes in aqueous solution and on the surfaces of glass substrates. Different from cotton and silk fabrics, the discharge effect of TDO directly depended on the compact structure of the polyester fibers. When a discharge accelerant was added into white discharge pastes with TDO, the compact structure of polyester fibers was opened up to achieve a good discharge effect. This study provides a new method for TDO development and a new strategy for the discharge printing of polyester fabrics.

Lightweight design of hinge beam based on Kriging agent model

Guizhong Xie,Shixin Zhang,Liangwen Wang,Xiaoyun Gong,Tao Wang,Shuguang Wang,Zhiqiang Chen,Zeheng Zhi 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

This paper presents an optimization method based on the Kriging and multiobjective genetic algorithm. First, taking the hinged beam structure of the cubic diamond press as the design object, the optimization design mathematical model was established with the mass as the objective function, the stress and displacement peak as the constraint conditions. Second, in combination with SolidWorks and ANSYS Workbench, parametric modeling analysis was conducted to obtain a large number of sample points sparing less time, and the agent model constructed by Kriging was trained and verified. Finally, taking advantage of global search of the multi-objective genetic algorithm, a lightweight design was realized and the mass of the hinge beam structure was effectively reduced, which would be a guiding significance for the lightweight design of other mechanical parts.

Co–Fe Bimetal Phosphate Composite Loaded on Reduced Graphene Oxide for Oxygen Evolution

Guoxing Zhu,Xulan Xie,Lisong Xiao,Xiaoyun Li,Xiaoping Shen,Yuanjun Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1

Development of high-performance nonprecious metal-based catalysts for oxygen evolution reaction (OER) is crucial to improve the efficiency of water electrolysis and photoelectrochemical water splitting for harvesting and storage of solar energy. Herein, Co–Fe phosphates and their composites with reduced graphene oxide (rGO) were prepared by a simple hydrothermal method, which then acted as oxygen evolution reaction catalysts. In 1.0 M KOH aqueous solution, the as-obtained optimal composite, Co–Fe phosphate/rGO, can catalyze oxygen evolution reaction with a very sharp onset potential and a small over-potential of 338 mV to achieve a current density of 10 mA cm -2. It was found that in these Co–Fe phosphates, the optimal Co:Fe ratio is 0.75:0.25. The excellent electrocatalytic performance of the Co–Fe phosphate/rGO composite would benefit from the synergistic effects between Fe and Co species, as well as rGO substrate providing conductive channels. The formed Co–Fe phosphate/rGO electrocatalysts can be the promising replacement of precious metal-based catalysts for more practical and cost-efficient water splitting.

Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues

Yuanming Shen,Xing Xie,Yang Li,Feng Ye,Fenfen Wang,Xiaoyun Wan,Weiguo Lu 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.6

Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However,few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA RT-qPCR studies. The aim of this study was to identify the most stable reference gene(s) for quantification of microRNA expression analysis in uterine cervical tissues. A microarray was performed on 6 pairs of uterine cervical tissues to identify the candidate reference genes. The stability of candidate reference genes was assessed by RT-qPCR in 23 pairs of uterine cervical tissues. The identified most stable reference genes were further validated in other cohort of 108 clinical uterine cervical samples:(HR-HPV- normal, n = 21; HR-HPV+ normal, n = 19; cervical intraepithelial neoplasia [CIN], n = 47; cancer, n = 21), and the effects of normalizers on the relative quantity of target miR-424 were assessed. In the array experiment, miR-26a, miR-23a, miR-200c, let-7a, and miR-1979 were identified as candidate reference genes for subsequent validation. MiR-23a was identified as the most reliable reference gene followed by miR-191. The use of miR-23a and miR-191 to normalize expression data enabled detection of a significant deregulation of miR-424 between normal, CIN and cancer tissue. Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer.

Exosomes from adipose-derived stem cells overexpressing Nrf2 accelerate cutaneous wound healing by promoting vascularization in a diabetic foot ulcer rat model

Xue-Li Zhou,Xiaoyun Xie,Weishuai Lian,Rongfeng Shi,Shilong Han,Haijun Zhang,Ligong Lu,Maoquan Li 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Diabetic foot ulcers (DFU) increase the risks of infection and amputation in patients with diabetes mellitus (DM). The impaired function and senescence of endothelial progenitor cells (EPCs) and high glucose-induced ROS likely exacerbate DFUs. We assessed EPCs in 60 patients with DM in a hospital or primary care setting. We also evaluated the therapeutic effects of exosomes secreted from adipose-derived stem cells (ADSCs) on stress-mediated senescence of EPCs induced by high glucose. Additionally, the effects of exosomes and Nrf2 overexpression in ADSCs were investigated in vitro and in vivo in a diabetic rat model. We found that ADSCs that secreted exosomes promoted proliferation and angiopoiesis in EPCs in a high glucose environment and that overexpression of Nrf2 increased this protective effect. Wounds in the feet of diabetic rats had a significantly reduced ulcerated area when treated with exosomes from ADSCs overexpressing Nrf2. Increased granulation tissue formation, angiogenesis, and levels of growth factor expression as well as reduced levels of inflammation and oxidative stress-related proteins were detected in wound beds. Our data suggest that exosomes from ADSCs can potentially promote wound healing, particularly when overexpressing Nrf2 and therefore that the transplantation of exosomes may be suitable for clinical application in the treatment of DFUs.

The Therapeutic Effects of Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells on Scleroderma

Yu Yue,Shen Liangliang,Xie Xiaoyun,Zhao Jingjun,Jiang Miao 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.1

BACKGROUND: Scleroderma is a multisystem disease in which tissue fibrosis is caused by inflammation and vascular damage. The mortality of scleroderma has remained high due to a lack of effective treatments. However, exosomes derived from human umbilical cord mesenchymal stem cells (HUMSCs)-Ex have been regarded as potential treatments for various autoimmune diseases, and may also act as candidates for treating scleroderma. METHODS: Mice with scleroderma received a single 50 lg HUMSCs-Ex. HUMSCs-Ex was characterized using transmission electron microscopy, nanoparticle tracking analysis and nanoflow cytometry. The therapeutic efficacy was assessed using histopathology, immunohistochemistry, immunofluorescence, quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay and western blot. RESULTS: HUMSCs-Ex ameliorated the deposition of extracellular matrix and suppressed the epithelial-mesenchymal transition process, and the effects lasted at least three weeks. In addition, HUMSCs-Ex promoted M1 macrophage polarization and inhibited M2 macrophage polarization, leading to the restoration of the balance of M1/M2 macrophages. CONCLUSION: We investigated the potential antifibrotic and anti-inflammatory effects of HUMSCs-Ex in a bleomycininduced mouse model of scleroderma. So HUMSCs-Ex could be considered as a candidate therapy for scleroderma.

Momordicine I alleviates isoproterenol-induced cardiomyocyte hypertrophy through suppression of PLA2G6 and DGK-ζ

Hongming Li,Yumei Qiu,Mengdie Xie,Changsheng Ouyang,Xiaoyun Ding,Hao Zhang,Wei Dong,Yinhua Xiong,Xilan Tang 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1

This study aimed to observe the protective effect of momordicine I, a triterpenoid compound extracted from momordica charantia L., on isoproterenol (ISO)-induced hypertrophy in rat H9c2 cardiomyocytes and investigate its potential mechanism. Treatment with 10 μM ISO induced cardiomyocyte hypertrophy as evidenced by increased cell surface area and protein content as well as pronounced upregulation of fetal genes including atrial natriuretic peptide, β-myosin heavy chain, and α-skeletal actin; however, those responses were markedly attenuated by treatment with 12.5 μg/ml momordicine I. Transcriptome experiment results showed that there were 381 and 447 differentially expressed genes expressed in comparisons of model/control and momordicine I intervention/model, respectively. GO enrichment analysis suggested that the anti-cardiomyocyte hypertrophic effect of momordicine I may be mainly associated with the regulation of metabolic processes. Based on our transcriptome experiment results as well as literature reports, we selected glycerophospholipid metabolizing enzymes group VI phospholipase A2 (PLA2G6) and diacylglycerol kinase ζ (DGK-ζ) as targets to further explore the potential mechanism through which momordicine I inhibited ISO-induced cardiomyocyte hypertrophy. Our results demonstrated that momordicine I inhibited ISO-induced upregulations of mRNA levels and protein expressions of PLA2G6 and DGK-ζ. Collectively, momordicine I alleviated ISO-induced cardiomyocyte hypertrophy, which may be related to its inhibition of the expression of glycerophospholipid metabolizing enzymes PLA2G6 and DGK-ζ.

Identification of miR-23a as a novel microRNA normalizer for relative quantification in human uterine cervical tissues

Shen, Yuanming,Li, Yang,Ye, Feng,Wang, Fenfen,Wan, Xiaoyun,Lu, Weiguo,Xie, Xing Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.6

Quantitative real-time RT-PCR (RT-qPCR) is being widely used in microRNA expression research. However, few reports detailed a robust identification and validation strategy for suitable reference genes for normalisation in microRNA RT-qPCR studies. The aim of this study was to identify the most stable reference gene(s) for quantification of microRNA expression analysis in uterine cervical tissues. A microarray was performed on 6 pairs of uterine cervical tissues to identify the candidate reference genes. The stability of candidate reference genes was assessed by RT-qPCR in 23 pairs of uterine cervical tissues. The identified most stable reference genes were further validated in other cohort of 108 clinical uterine cervical samples: (HR-HPV- normal, n = 21; HR-HPV+ normal, n = 19; cervical intraepithelial neoplasia [CIN], n = 47; cancer, n = 21), and the effects of normalizers on the relative quantity of target miR-424 were assessed. In the array experiment, miR-26a, miR-23a, miR-200c, let-7a, and miR-1979 were identified as candidate reference genes for subsequent validation. MiR-23a was identified as the most reliable reference gene followed by miR-191. The use of miR-23a and miR-191 to normalize expression data enabled detection of a significant deregulation of miR-424 between normal, CIN and cancer tissue. Our results suggested that miR-23a and miR-191 are the optimal reference microRNAs that can be used for normalization in profiling studies of cervical tissues; miR-23a is a novel microRNA normalizer.